Abstract:
:In endothelial cells specifically, cPLA2alpha translocates from the cytoplasm to the Golgi complex in response to cell confluence. Considering the link between confluence and cell-cell junction formation, and the emerging role of cPLA2alpha in intracellular trafficking, we tested whether Golgi-associated cPLA2alpha is involved in the trafficking of junction proteins. Here, we show that the redistribution of cPLA2alpha from the cytoplasm to the Golgi correlates with adherens junction maturation and occurs before tight junction formation. Disruption of adherens junctions using a blocking anti-VE-cadherin antibody reverses the association of cPLA2alpha with the Golgi. Silencing of cPLA2alpha and inhibition of cPLA2alpha enzymatic activity using various inhibitors result in the diminished presence of the transmembrane junction proteins VE-cadherin, occludin, and claudin-5 at cell-cell contacts, and in their accumulation at the Golgi. Altogether, our data support the idea that VE-cadherin triggers the relocation of cPLA2alpha to the Golgi and that in turn, Golgi-associated cPLA2alpha regulates the transport of transmembrane junction proteins through or from the Golgi, thereby controlling the integrity of endothelial cell-cell junctions.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Regan-Klapisz E,Krouwer V,Langelaar-Makkinje M,Nallan L,Gelb M,Gerritsen H,Verkleij AJ,Post JAdoi
10.1091/mbc.e08-02-0210subject
Has Abstractpub_date
2009-10-01 00:00:00pages
4225-34issue
19eissn
1059-1524issn
1939-4586pii
E08-02-0210journal_volume
20pub_type
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