What makes the cell cycle tick? a celebration of the awesome power of biochemistry and the frog egg.

abstract：:ATP8A2 is a P4-ATPase that flips phosphatidylserine and phosphatidylethanolamine across cell membranes. This generates membrane phospholipid asymmetry, a property important in many cellular processes, including vesicle trafficking. ATP8A2 deficiency causes severe neurodegenerative diseases. We investigated the role of...

journal_title：Molecular biology of the cell

authors： Chalat M,Moleschi K,Molday RS

更新日期：2017-02-01 00:00:00

abstract：:Terminal regions of Drosophila embryos are patterned by signaling through ERK, which is genetically deregulated in multiple human diseases. Quantitative studies of terminal patterning have been recently used to investigate gain-of-function variants of human MEK1, encoding the MEK kinase that directly activates ERK by ...

journal_title：Molecular biology of the cell

authors： Marmion RA,Yang L,Goyal Y,Jindal GA,Wetzel JL,Singh M,Schüpbach T,Shvartsman SY

更新日期：2021-01-21 00:00:00

abstract：:Gpi8p and Gaa1p are essential components of the GPI transamidase that adds glycosylphosphatidylinositols (GPIs) to newly synthesized proteins. After solubilization in 1.5% digitonin and separation by blue native PAGE, Gpi8p is found in 430-650-kDa protein complexes. These complexes can be affinity purified and are sho...

journal_title：Molecular biology of the cell

authors： Fraering P,Imhof I,Meyer U,Strub JM,van Dorsselaer A,Vionnet C,Conzelmann A

更新日期：2001-10-01 00:00:00

abstract：:Endocytosis modulates EGFR function by compartmentalizing and attenuating or enhancing its ligand-induced signaling. Here we show that it can also control the cell surface versus intracellular distribution of empty/inactive EGFR. Our previous observation that PKA inhibitors induce EGFR internalization prompted us to t...

journal_title：Molecular biology of the cell

authors： Norambuena A,Metz C,Jung JE,Silva A,Otero C,Cancino J,Retamal C,Valenzuela JC,Soza A,González A

更新日期：2010-08-15 00:00:00

abstract：:During mating, Saccharomyces cerevisiae cells must degrade the intervening cell wall to allow fusion of the partners. Because improper timing or location of cell wall degradation would cause lysis, the initiation of cell fusion must be highly regulated. Here, we find that yeast cell fusion is negatively regulated by c...

journal_title：Molecular biology of the cell

authors： Hall AE,Rose MD

更新日期：2019-02-15 00:00:00

abstract：:Genes occupy preferred spatial positions within interphase cell nuclei. However, positioning patterns are not an innate feature of a locus, and genes can alter their localization in response to physiological and pathological changes. Here we screen the radial positioning patterns of 40 genes in normal, hyperplasic, an...

journal_title：Molecular biology of the cell

authors： Leshner M,Devine M,Roloff GW,True LD,Misteli T,Meaburn KJ

更新日期：2016-01-15 00:00:00

abstract：:The AP-2 adaptor complex plays a key role in cargo recognition and clathrin-coated vesicle formation at the plasma membrane. To investigate the functions of individual binding sites and domains of the AP-2 complex in vivo, we have stably transfected HeLa cells with wild-type and mutant small interfering RNA-resistant ...

journal_title：Molecular biology of the cell

authors： Motley AM,Berg N,Taylor MJ,Sahlender DA,Hirst J,Owen DJ,Robinson MS

更新日期：2006-12-01 00:00:00

abstract：:Proper chromosome segregation is essential for faithful cell division and if not maintained results in defective cell function caused by the abnormal distribution of genetic information. Polo-like kinase 1-interacting checkpoint helicase (PICH) is a DNA translocase essential for chromosome bridge resolution during mit...

journal_title：Molecular biology of the cell

authors： Hassebroek VA,Park H,Pandey N,Lerbakken BT,Aksenova V,Arnaoutov A,Dasso M,Azuma Y

更新日期：2020-11-01 00:00:00

abstract：:The importance of cholesterol for endocytosis has been investigated in HEp-2 and other cell lines by using methyl-beta-cyclodextrin (MbetaCD) to selectively extract cholesterol from the plasma membrane. MbetaCD treatment strongly inhibited endocytosis of transferrin and EGF, whereas endocytosis of ricin was less affec...

journal_title：Molecular biology of the cell

authors： Rodal SK,Skretting G,Garred O,Vilhardt F,van Deurs B,Sandvig K

更新日期：1999-04-01 00:00:00

abstract：:Many yeast actin cytoskeleton mutants accumulate large secretory vesicles and exhibit phenotypes consistent with defects in polarized growth. This, together with actin's polarized organization, has suggested a role for the actin cytoskeleton in the vectorial transport of late secretory vesicles to the plasma membrane....

journal_title：Molecular biology of the cell

authors： Mulholland J,Wesp A,Riezman H,Botstein D

更新日期：1997-08-01 00:00:00

abstract：:Metaphase spindles assemble to a steady state in length by mechanisms that involve microtubule dynamics and motor proteins, but they are incompletely understood. We found that Xenopus extract spindles recapitulate the length of egg meiosis II spindles, by using mechanisms intrinsic to the spindle. To probe these mecha...

journal_title：Molecular biology of the cell

authors： Mitchison TJ,Maddox P,Gaetz J,Groen A,Shirasu M,Desai A,Salmon ED,Kapoor TM

更新日期：2005-06-01 00:00:00

abstract：:The mechanisms and receptors involved in phagocytosis by nonhematopoietic cells are not well understood. The involvement of the alpha 3 beta 1 integrin in phagocytosis of the extracellular matrix by human breast cancer cells was studied. The possible role of this integrin was suggested since alpha 3 and beta 1 but not...

journal_title：Molecular biology of the cell

authors： Coopman PJ,Thomas DM,Gehlsen KR,Mueller SC

更新日期：1996-11-01 00:00:00

abstract：:To date, two major apoptotic pathways, the death receptor and the mitochondrial pathway, have been well documented in mammalian cells. However, the involvement of these two apoptotic pathways, particularly the death receptor pathway, in transforming growth factor-beta 1 (TGF-beta 1)-induced apoptosis is not well under...

journal_title：Molecular biology of the cell

authors： Kim SG,Jong HS,Kim TY,Lee JW,Kim NK,Hong SH,Bang YJ

更新日期：2004-02-01 00:00:00

abstract：:Rab proteins are thought to function in the processes by which transport vesicles identify and/or fuse with their respective target membranes. The bulk of these proteins are membrane associated, but a measurable fraction can be found in the cytosol. The cytosolic forms of rab3A, rab11, and Sec4 occur as equimolar comp...

journal_title：Molecular biology of the cell

authors： Soldati T,Riederer MA,Pfeffer SR

更新日期：1993-04-01 00:00:00

abstract：:Macrophage fusion resulting in the formation of multinucleated giant cells occurs in a variety of chronic inflammatory diseases, yet the mechanism responsible for initiating this process is unknown. Here, we used live cell imaging to show that actin-based protrusions at the leading edge initiate macrophage fusion. Pha...

journal_title：Molecular biology of the cell

authors： Faust JJ,Balabiyev A,Heddleston JM,Podolnikova NP,Baluch DP,Chew TL,Ugarova TP

更新日期：2019-08-01 00:00:00

abstract：:Polarized cell growth requires the coupling of a defined spatial site on the cell cortex to the apparatus that directs the establishment of cell polarity. In the budding yeast Saccharomyces cerevisiae, the Ras-family GTPase Rsr1p/Bud1p and its regulators select the proper site for bud emergence on the cell cortex. The...

journal_title：Molecular biology of the cell

authors： Kozminski KG,Beven L,Angerman E,Tong AH,Boone C,Park HO

更新日期：2003-12-01 00:00:00

abstract：:Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are...

journal_title：Molecular biology of the cell

authors： Gutiérrez-Escribano P,González-Novo A,Suárez MB,Li CR,Wang Y,de Aldana CR,Correa-Bordes J

更新日期：2011-07-15 00:00:00

abstract：:The E3 ubiquitin ligase Rad18 chaperones DNA polymerase η (Polη) to sites of UV-induced DNA damage and monoubiquitinates proliferating cell nuclear antigen (PCNA), facilitating engagement of Polη with stalled replication forks and promoting translesion synthesis (TLS). It is unclear how Rad18 activities are coordinate...

journal_title：Molecular biology of the cell

authors： Barkley LR,Palle K,Durando M,Day TA,Gurkar A,Kakusho N,Li J,Masai H,Vaziri C

更新日期：2012-05-01 00:00:00

abstract：:Microtubules (MTs) are essential for cell division, shape, intracellular transport, and polarity. MT stability is regulated by many factors, including MT-associated proteins and proteins controlling the amount of free tubulin heterodimers available for polymerization. Tubulin-binding cofactors are potential key regula...

journal_title：Molecular biology of the cell

authors： Baffet AD,Benoit B,Januschke J,Audo J,Gourhand V,Roth S,Guichet A

更新日期：2012-09-01 00:00:00

abstract：:In most eukaryotes, genes encoding ribosomal RNAs (rDNA) are clustered in long tandem head-to-tail repeats. Studies of Saccharomyces cerevisiae have indicated that rDNA copy number is maintained through recombination events associated with site-specific blockage of replication forks (RFs). Here, we describe two Schizo...

journal_title：Molecular biology of the cell

authors： Coulon S,Gaillard PHL,Chahwan C,McDonald WH,Yates JR 3rd,Russell P

更新日期：2004-01-01 00:00:00

abstract：:Fission yeast rad22(+), a homologue of budding yeast RAD52, encodes a double-strand break repair component, which is dispensable for proliferation. We, however, have recently obtained a cell division cycle mutant with a temperature-sensitive allele of rad22(+), designated rad22-H6, which resulted from a point mutation...

journal_title：Molecular biology of the cell

authors： Suto K,Nagata A,Murakami H,Okayama H

更新日期：1999-10-01 00:00:00

abstract：:In eukaryotic cells, organelle-specific protein quality control (PQC) is critical for maintaining cellular homeostasis. Despite the Golgi apparatus being the major protein processing and sorting site within the secretory pathway, how it contributes to PQC has remained largely unknown. Using different chemical biology-...

journal_title：Molecular biology of the cell

authors： Hellerschmied D,Serebrenik YV,Shao L,Burslem GM,Crews CM

更新日期：2019-08-01 00:00:00

abstract：:MicroRNAs (miRNAs), the tiny regulatory RNAs, form complexes with Argonaute (Ago) proteins and inhibit gene expression in metazoan cells. While studying parasite-invaded macrophages, we identify a unique mode of gene regulation in which the parasite Leishmania donovani (Ld) causes mitochondrial depolarization, reduces...

journal_title：Molecular biology of the cell

authors： Chakrabarty Y,Bhattacharyya SN

更新日期：2017-07-15 00:00:00

abstract：:In kinetochores, the Ndc80 complex couples the energy in a depolymerizing microtubule to perform the work of moving chromosomes. The complex directly binds microtubules using an unstructured, positively charged N-terminal tail located on Hec1/Ndc80. Hec1/Ndc80 also contains a calponin homology domain (CHD) that increa...

journal_title：Molecular biology of the cell

authors： Tooley JG,Miller SA,Stukenberg PT

更新日期：2011-04-15 00:00:00

abstract：:UNC-104 (KIF1A) is a kinesin motor that transports synaptic vesicles from the neuronal cell body to the terminal. Previous in vitro studies have shown that a Dictyostelium relative of UNC-104 transports liposomes containing acidic phospholipids, but whether this interaction is needed for the recognition and transport ...

journal_title：Molecular biology of the cell

authors： Klopfenstein DR,Vale RD

更新日期：2004-08-01 00:00:00

abstract：:Compositional analyses of nucleic acids and proteins have shed light on possible origins of living cells. In this work, rigorous compositional analyses of ∼5000 plasma membrane lipid constituents of 273 species in the three life domains (archaea, eubacteria, and eukaryotes) revealed a remarkable statistical paradox, i...

journal_title：Molecular biology of the cell

authors： Bansal S,Mittal A

更新日期：2015-04-01 00:00:00

abstract：:Transport of cellular and neuronal vesicles, organelles, and other particles along microtubules requires the molecular motor protein dynein (Mallik and Gross, 2004). Critical to dynein function is dynactin, a multiprotein complex commonly thought to be required for dynein attachment to membrane compartments (Karki and...

journal_title：Molecular biology of the cell

authors： Haghnia M,Cavalli V,Shah SB,Schimmelpfeng K,Brusch R,Yang G,Herrera C,Pilling A,Goldstein LS

更新日期：2007-06-01 00:00:00

abstract：:EB1 is a conserved microtubule plus end tracking protein considered to play crucial roles in microtubule organization and the interaction of microtubules with the cell cortex. Despite intense studies carried out in yeast and cultured cells, the role of EB1 in multicellular systems remains to be elucidated. Here, we de...

journal_title：Molecular biology of the cell

authors： Elliott SL,Cullen CF,Wrobel N,Kernan MJ,Ohkura H

更新日期：2005-02-01 00:00:00

abstract：:Triggering receptor expressed on myeloid cells 2 (TREM2) is a transmembrane protein expressed on microglia within the brain. Several rare mutations in TREM2 cause an early-onset form of neurodegeneration when inherited homozygously. Here we investigate how these mutations affect the intracellular transport of TREM2. W...

journal_title：Molecular biology of the cell

authors： Sirkis DW,Aparicio RE,Schekman R

更新日期：2017-10-01 00:00:00

abstract：:Survivin, a subunit of the chromosome passenger complex (CPC), binds the N-terminal tail of histone H3, which is phosphorylated on T3 by Haspin kinase, and localizes the complex to the inner centromeres. We used x-ray crystallography to determine the residues of Survivin that are important in binding phosphomodified h...

journal_title：Molecular biology of the cell

authors： Niedzialkowska E,Wang F,Porebski PJ,Minor W,Higgins JM,Stukenberg PT

更新日期：2012-04-01 00:00:00