The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo.

abstract：:The Ste5 scaffold activates an associated mitogen-activated protein kinase cascade by binding through its RING-H2 domain to a Gbetagamma dimer (Ste4/Ste18) at the plasma membrane in a recruitment event that requires prior nuclear shuttling of Ste5. Genetic evidence suggests that Ste5 must oligomerize to function, but ...

journal_title：Molecular biology of the cell

authors： Wang Y,Elion EA

更新日期：2003-06-01 00:00:00

abstract：:Microtubules (MTs) have been proposed to act mechanically as compressive struts that resist both actomyosin contractile forces and their own polymerization forces to mechanically stabilize cell shape. To identify the origin of MT bending, we directly observed MT bending and F-actin transport dynamics in the periphery ...

journal_title：Molecular biology of the cell

authors： Bicek AD,Tüzel E,Demtchouk A,Uppalapati M,Hancock WO,Kroll DM,Odde DJ

更新日期：2009-06-01 00:00:00

abstract：:Protein degradation during the cell cycle is controlled by the opposing activities of ubiquitin ligases and deubiquitinating enzymes (DUBs). Although the functions of ubiquitin ligases in the cell cycle have been studied extensively, the roles of DUBs in this process are less well understood. Here, we used an overexpr...

journal_title：Molecular biology of the cell

authors： Mapa CE,Arsenault HE,Conti MM,Poti KE,Benanti JA

更新日期：2018-11-15 00:00:00

abstract：:Functionally different subsets of actin filament arrays contribute to cellular organization and motility. We report the identification of a novel subset of loose actin filament arrays through regulated association with the widely expressed protein SWAP-70. These loose actin filament arrays were commonly located behind...

journal_title：Molecular biology of the cell

authors： Hilpelä P,Oberbanscheidt P,Hahne P,Hund M,Kalhammer G,Small JV,Bähler M

更新日期：2003-08-01 00:00:00

abstract：:The function currently attributed to tetraspanins is to organize molecular complexes in the plasma membrane by using multiple cis-interactions. Additionally, the tetraspanin CD9 may be a receptor that binds the soluble ligand PSG17, a member of the immunoglobulin superfamily (IgSF)/CEA subfamily. However, previous dat...

journal_title：Molecular biology of the cell

authors： Ellerman DA,Ha C,Primakoff P,Myles DG,Dveksler GS

更新日期：2003-12-01 00:00:00

abstract：:Differentiation-inducing factor-1 (DIF-1) is a polyketide that induces Dictyostelium amoebae to differentiate as prestalk cells. We performed a global quantitative screen for phosphorylation changes that occur within the first minutes after addition of DIF-1, using a triple-label SILAC approach. This revealed a new wo...

journal_title：Molecular biology of the cell

authors： Sugden C,Urbaniak MD,Araki T,Williams JG

更新日期：2015-02-15 00:00:00

abstract：:Tetraploidization, or genome doubling, is a prominent event in tumorigenesis, primarily because cell division in polyploid cells is error-prone and produces aneuploid cells. This study investigates changes in gene expression evoked in acute and adapted tetraploid cells and their effect on cell-cycle progression. Acute...

journal_title：Molecular biology of the cell

authors： Potapova TA,Seidel CW,Box AC,Rancati G,Li R

更新日期：2016-10-15 00:00:00

abstract：:A symposium was held at the University of California, San Diego, to honor the contributions of Nobel Laureate, George Palade, to cell biology. The speakers included Günter Blobel, on the structure and function of nuclear pore complexes; Peter Walter, on the unfolded protein response in health and disease; Randy Schekm...

journal_title：Molecular biology of the cell

authors： Schmid SL,Farquhar MG

更新日期：2010-07-15 00:00:00

abstract：:Heterotrimeric G proteins are components of principal signaling pathways in eukaryotes. In higher organisms, alpha subunits of G proteins have been divided into four families, Gi, Gs, Gq, and G12. We previously identified a G alpha i homologue gna-1 in the filamentous fungus Neurospora crassa. Now we report that delet...

journal_title：Molecular biology of the cell

authors： Ivey FD,Hodge PN,Turner GE,Borkovich KA

更新日期：1996-08-01 00:00:00

abstract：:The maturation of dendritic cells is accompanied by the redistribution of major histocompatibility complex (MHC) class II molecules from the lysosomal MHC class II compartment to the plasma membrane to mediate presentation of peptide antigens. Besides MHC molecules, dendritic cells also express CD1 molecules that medi...

journal_title：Molecular biology of the cell

authors： van der Wel NN,Sugita M,Fluitsma DM,Cao X,Schreibelt G,Brenner MB,Peters PJ

更新日期：2003-08-01 00:00:00

abstract：:Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate r...

journal_title：Molecular biology of the cell

authors： Holenstein CN,Horvath A,Schär B,Schoenenberger AD,Bollhalder M,Goedecke N,Bartalena G,Otto O,Herbig M,Guck J,Müller DA,Snedeker JG,Silvan U

更新日期：2019-03-21 00:00:00

abstract：:Nuclear migration is required for normal development in both higher and lower eukaryotes. In fungi this process is mediated by cytoplasmic dynein. It is believed that this motor protein is anchored to the cell membrane and moves nuclei by capturing and pulling on spindle pole body microtubules. To date, four genes hav...

journal_title：Molecular biology of the cell

authors： Chiu YH,Xiang X,Dawe AL,Morris NR

更新日期：1997-09-01 00:00:00

abstract：:Cholesterol transport is an essential process in all multicellular organisms. In this study we applied two recently developed approaches to investigate the distribution and molecular mechanisms of cholesterol transport in Caenorhabditis elegans. The distribution of cholesterol in living worms was studied by imaging it...

journal_title：Molecular biology of the cell

authors： Matyash V,Geier C,Henske A,Mukherjee S,Hirsh D,Thiele C,Grant B,Maxfield FR,Kurzchalia TV

更新日期：2001-06-01 00:00:00

abstract：:The Cvt pathway is a biosynthetic transport route for a distinct subset of resident yeast vacuolar hydrolases, whereas macroautophagy is a nonspecific degradative mechanism that allows cell survival during starvation. Yet, these two vacuolar trafficking pathways share a number of identical molecular components and are...

journal_title：Molecular biology of the cell

authors： Reggiori F,Wang CW,Nair U,Shintani T,Abeliovich H,Klionsky DJ

更新日期：2004-05-01 00:00:00

abstract：:Regulation of Rho GTPase signaling is critical for cell shape determination and polarity. Here, we investigated the role of LRG1, a novel member of the GTPase-activating proteins (GAPs) of Neurospora crassa. LRG1 is essential for apical tip extension and to restrict excessive branch formation in subapical regions of t...

journal_title：Molecular biology of the cell

authors： Vogt N,Seiler S

更新日期：2008-11-01 00:00:00

abstract：:Previous research has found that a gamma-tubulin mutation in Schizosaccharomyces pombe is synthetically lethal with a deletion of the C-terminal motor domain kinesin-like protein gene pkl1, but the lethality of the double mutant prevents a phenotypic analysis of the synthetic interaction. We have investigated interact...

journal_title：Molecular biology of the cell

authors： Prigozhina NL,Walker RA,Oakley CE,Oakley BR

更新日期：2001-10-01 00:00:00

abstract：:The mitochondrial cytochrome c oxidase assembles in the inner membrane from subunits of dual genetic origin. The assembly process of the enzyme is initiated by membrane insertion of the mitochondria-encoded Cox1 subunit. During complex maturation, transient assembly intermediates, consisting of structural subunits and...

journal_title：Molecular biology of the cell

authors： Bareth B,Nikolov M,Lorenzi I,Hildenbeutel M,Mick DU,Helbig C,Urlaub H,Ott M,Rehling P,Dennerlein S

更新日期：2016-05-15 00:00:00

abstract：:Breast epithelial cells cultured in three-dimensional (3D) collagen gels undergo ductal morphogenesis when the gel is compliant and they can achieve tensional homeostasis. We previously showed that this process requires down-regulation of Rho in compliant collagen gels, but the mechanism remains undefined. In this stu...

journal_title：Molecular biology of the cell

authors： Ponik SM,Trier SM,Wozniak MA,Eliceiri KW,Keely PJ

更新日期：2013-06-01 00:00:00

abstract：:The histone variant H2AX is rapidly phosphorylated at the sites of DNA double-strand breaks (DSBs). This phosphorylated H2AX (gamma-H2AX) is involved in the retention of repair and signaling factor complexes at sites of DNA damage. The dependency of this phosphorylation on the various PI3K-related protein kinases (in ...

journal_title：Molecular biology of the cell

authors： Friesner JD,Liu B,Culligan K,Britt AB

更新日期：2005-05-01 00:00:00

abstract：:The Drosophila melanogaster genes Hrb87F and Hrb98DE encode the fly proteins HRB87F and HRB98DE (also known as hrp36 and hrp38, respectively) that are most similar in sequence and function to mammalian A/B-type hnRNP proteins. Using overexpression and deletion mutants of Hrb87F, we have tested the hypothesis that the ...

journal_title：Molecular biology of the cell

authors： Zu K,Sikes ML,Haynes SR,Beyer AL

更新日期：1996-07-01 00:00:00

abstract：:The crystalline lens is an attractive system to study the biology of intercellular communication; however, the identity of the structural components of gap junctions in the lens has been controversial. We have cloned a novel member of the connexin family of gap junction proteins, Cx50, and have shown that it is likely...

journal_title：Molecular biology of the cell

authors： White TW,Bruzzone R,Goodenough DA,Paul DL

更新日期：1992-07-01 00:00:00

abstract：:Although evasion of apoptosis is thought to be required for the development of cancer, it is unclear which cell death pathways are evaded. We previously identified a novel epithelial cell death pathway that works in normal cells but is inactivated in tumor cells, implying that it may be targeted during tumor developme...

journal_title：Molecular biology of the cell

authors： Thorburn J,Moore F,Rao A,Barclay WW,Thomas LR,Grant KW,Cramer SD,Thorburn A

更新日期：2005-03-01 00:00:00

abstract：:Alternative pre-mRNA splicing patterns can change an extracellular stimulus, but the signaling pathways leading to these changes are still poorly characterized. Here, we describe a tyrosine-phosphorylated nuclear protein, YT521-B, and show that it interacts with the nuclear transcriptosomal component scaffold attachme...

journal_title：Molecular biology of the cell

authors： Hartmann AM,Nayler O,Schwaiger FW,Obermeier A,Stamm S

更新日期：1999-11-01 00:00:00

abstract：:Polypeptides targeted to the yeast endoplasmic reticulum (ER) posttranslationally are thought to be kept in the cytoplasm in an unfolded state by Hsp70 chaperones before translocation. We show here that Escherichia coli beta-lactamase associated with Hsp70, but adopted a native-like conformation before translocation i...

journal_title：Molecular biology of the cell

authors： Paunola E,Suntio T,Jämsä E,Makarow M

更新日期：1998-04-01 00:00:00

abstract：:The sorting of most integral membrane proteins into the lumenal vesicles of multivesicular bodies (MVBs) is dependent on the attachment of ubiquitin (Ub) to their cytosolic domains. However, Ub is not required for sorting of Sna3, an MVB vesicle cargo protein in yeast. We show that Sna3 circumvents Ub-mediated recogni...

journal_title：Molecular biology of the cell

authors： McNatt MW,McKittrick I,West M,Odorizzi G

更新日期：2007-02-01 00:00:00

abstract：:In response to stress conditions (such as nutrient limitation or accumulation of damaged organelles) and certain pathological situations, eukaryotic cells use autophagy as a survival mechanism. During nutrient stress the main purpose of autophagy is to degrade cytoplasmic materials within the lysosome/vacuole lumen an...

journal_title：Molecular biology of the cell

authors： Yang Z,Huang J,Geng J,Nair U,Klionsky DJ

更新日期：2006-12-01 00:00:00

abstract：:Three mitochondrial DNA-encoded proteins, Cox1, Cox2, and Cox3, comprise the core of the cytochrome c oxidase complex. Gene-specific translational activators ensure that these respiratory chain subunits are synthesized at the correct location and in stoichiometric ratios to prevent unassembled protein products from ge...

journal_title：Molecular biology of the cell

authors： Roloff GA,Henry MF

更新日期：2015-08-15 00:00:00

abstract：:Multisite phosphorylation of proteins is a powerful signal processing mechanism that plays crucial roles in cell division and differentiation as well as in disease. We recently demonstrated a novel phenomenon in cell cycle regulation by showing that cyclin-dependent kinase-dependent multisite phosphorylation of a cruc...

journal_title：Molecular biology of the cell

authors： Valk E,Venta R,Ord M,Faustova I,Kõivomägi M,Loog M

更新日期：2014-11-05 00:00:00

abstract：:SPOP, speckle-type POZ protein is a substrate adaptor protein of the Cullin-3/RING ubiquitin E3 complex. The spop gene is the most commonly point mutated in human primary prostate cancers, but the pathological contribution of the SPOP mutations remains unclear. In this study, we investigated several known factors that...

journal_title：Molecular biology of the cell

authors： Watanabe R,Maekawa M,Hieda M,Taguchi T,Miura N,Kikugawa T,Saika T,Higashiyama S

更新日期：2020-03-15 00:00:00

abstract：:During mating, Saccharomyces cerevisiae cells must degrade the intervening cell wall to allow fusion of the partners. Because improper timing or location of cell wall degradation would cause lysis, the initiation of cell fusion must be highly regulated. Here, we find that yeast cell fusion is negatively regulated by c...

journal_title：Molecular biology of the cell

authors： Hall AE,Rose MD

更新日期：2019-02-15 00:00:00