SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.

abstract：:We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Se...

journal_title：Molecular biology of the cell

authors： Denegri M,Moralli D,Rocchi M,Biggiogera M,Raimondi E,Cobianchi F,De Carli L,Riva S,Biamonti G

更新日期：2002-06-01 00:00:00

abstract：:I am extremely honored to be the recipient of the 2015 Women in Cell Biology Junior Award. When I reflect on my journey in science, many great people and memorable experiences come to mind. Some of these encounters were truly career-defining moments. Others provided priceless lessons. In this essay, I recount some of ...

journal_title：Molecular biology of the cell

authors： Serpe M

更新日期：2015-11-01 00:00:00

abstract：:Androgen receptor (AR) belongs to the nuclear receptor superfamily and mediates the biological actions of male sex steroids. In this work, we have characterized a novel 130-kDa Ser/Thr protein kinase ANPK that interacts with the zinc finger region of AR in vivo and in vitro. The catalytic kinase domain of ANPK shares ...

journal_title：Molecular biology of the cell

authors： Moilanen AM,Karvonen U,Poukka H,Jänne OA,Palvimo JJ

更新日期：1998-09-01 00:00:00

abstract：:Sec13p has been thought to be an essential component of the COPII coat, required for exit of proteins from the yeast endoplasmic reticulum (ER). We show herein that normal function of Sec13p was not required for ER exit of the Hsp150 glycoprotein. Hsp150 was secreted to the medium under restrictive conditions in a sec...

journal_title：Molecular biology of the cell

authors： Fatal N,Suntio T,Makarow M

更新日期：2002-12-01 00:00:00

abstract：:Members of the transforming acidic coiled coil (TACC) protein family are emerging as important mitotic spindle assembly proteins in a variety of organisms. The molecular details of how TACC proteins function are unknown, but TACC proteins have been proposed to recruit microtubule-stabilizing proteins of the tumor over...

journal_title：Molecular biology of the cell

authors： Albee AJ,Wiese C

更新日期：2008-08-01 00:00:00

abstract：:Transforming growth factor-β (TGF-β) plays critical roles in numerous physiological and pathological responses. Cholesterol, a major plasma membrane component, can have pronounced effects on signaling responses. Cells continually monitor cholesterol content and activate multilayered transcriptional and translational s...

journal_title：Molecular biology of the cell

authors： Shapira KE,Ehrlich M,Henis YI

更新日期：2018-10-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is caused by homozygous mutations in human SMN1 Expression of a duplicate gene (SMN2) primarily results in skipping of exon 7 and production of an unstable protein isoform, SMNΔ7. Although SMN2 exon skipping is the principal contributor to SMA severity, mechanisms governing stability of s...

journal_title：Molecular biology of the cell

authors： Gray KM,Kaifer KA,Baillat D,Wen Y,Bonacci TR,Ebert AD,Raimer AC,Spring AM,Have ST,Glascock JJ,Gupta K,Van Duyne GD,Emanuele MJ,Lamond AI,Wagner EJ,Lorson CL,Matera AG

更新日期：2018-01-15 00:00:00

abstract：:Breast epithelial cells cultured in three-dimensional (3D) collagen gels undergo ductal morphogenesis when the gel is compliant and they can achieve tensional homeostasis. We previously showed that this process requires down-regulation of Rho in compliant collagen gels, but the mechanism remains undefined. In this stu...

journal_title：Molecular biology of the cell

authors： Ponik SM,Trier SM,Wozniak MA,Eliceiri KW,Keely PJ

更新日期：2013-06-01 00:00:00

abstract：:The preprotein translocase of the outer mitochondrial membrane (TOM) consists of a central β-barrel channel, Tom40, and six proteins with α-helical transmembrane segments. The precursor of Tom40 is imported from the cytosol by a pre-existing TOM complex and inserted into the outer membrane by the sorting and assembly ...

journal_title：Molecular biology of the cell

authors： Becker T,Guiard B,Thornton N,Zufall N,Stroud DA,Wiedemann N,Pfanner N

更新日期：2010-09-15 00:00:00

abstract：:We have identified and characterized sorb-1, the only sorbin and SH3 domain-containing protein family member in Caenorhabditis elegans SORB-1 is strongly localized to integrin adhesion complexes in larvae and adults, including adhesion plaques and dense bodies (Z-disks) of striated muscles and attachment plaques of sm...

journal_title：Molecular biology of the cell

authors： Loveless T,Qadota H,Benian GM,Hardin J

更新日期：2017-12-01 00:00:00

abstract：:The control of muscle-specific expression is one of the principal mechanisms by which diversity is generated among muscle types. In an attempt to elucidate the regulatory mechanisms that control fiber diversity in any given muscle, we have focused our attention on the transcriptional regulation of the Drosophila Tropo...

journal_title：Molecular biology of the cell

authors： Mas JA,García-Zaragoza E,Cervera M

更新日期：2004-04-01 00:00:00

abstract：:Gp78 is an ERAD-associated E3 ubiquitin ligase that induces degradation of the mitofusin mitochondrial fusion proteins and mitochondrial fission. Gp78 is localized throughout the ER; however, the anti-Gp78 3F3A monoclonal antibody (mAb) recognizes Gp78 selectively in mitochondria-associated ER domains. Epitope mapping...

journal_title：Molecular biology of the cell

authors： Li L,Gao G,Shankar J,Joshi B,Foster LJ,Nabi IR

更新日期：2015-11-01 00:00:00

abstract：:Although p38 activity is reported to be required as cells enter mitosis for proper spindle assembly and checkpoint function, its role during the division process remains controversial in lieu of direct data. We therefore conducted live cell studies to determine the effect on mitosis of inhibiting or depleting p38. We ...

journal_title：Molecular biology of the cell

authors： Lee K,Kenny AE,Rieder CL

更新日期：2010-07-01 00:00:00

abstract：:The clinical interest in farnesyltransferase inhibitors (FTIs) makes it important to understand how these compounds affect cellular processes involving farnesylated proteins. Mitotic abnormalities observed after treatment with FTIs have so far been attributed to defects in the farnesylation of the outer kinetochore pr...

journal_title：Molecular biology of the cell

authors： Holland AJ,Reis RM,Niessen S,Pereira C,Andres DA,Spielmann HP,Cleveland DW,Desai A,Gassmann R

更新日期：2015-05-15 00:00:00

abstract：:Ubiquitination of proliferating cell nuclear antigen (PCNA) plays a crucial role in regulating replication past DNA damage in eukaryotes, but the detailed mechanisms appear to vary in different organisms. We have examined the modification of PCNA in Schizosaccharomyces pombe. We find that, in response to UV irradiatio...

journal_title：Molecular biology of the cell

authors： Frampton J,Irmisch A,Green CM,Neiss A,Trickey M,Ulrich HD,Furuya K,Watts FZ,Carr AM,Lehmann AR

更新日期：2006-07-01 00:00:00

abstract：:Soluble N-ethyl maleimide-sensitive fusion protein attachment protein receptors (SNAREs) are core machinery for membrane fusion during intracellular vesicular transport. Synaptosome-associated protein of 23 kDa (SNAP23) is a target SNARE previously identified at the plasma membrane, where it is involved in exocytotic ...

journal_title：Molecular biology of the cell

authors： Faigle W,Colucci-Guyon E,Louvard D,Amigorena S,Galli T

更新日期：2000-10-01 00:00:00

abstract：:During the course of a bacterial infection, cells are exposed simultaneously to a range of bacterial and host factors, which converge on the central transcription factor nuclear factor (NF)-κB. How do single cells integrate and process these converging stimuli? Here we tackle the question of how cells process combinat...

journal_title：Molecular biology of the cell

authors： Gutschow MV,Mason JC,Lane KM,Maayan I,Hughey JJ,Bajar BT,Amatya DN,Valle SD,Covert MW

更新日期：2019-01-15 00:00:00

abstract：:Bile ducts are hepatic tubular structures that are lined by cholangiocytes, a type of liver epithelial cell. Cholangiocytes first form a single layer of cells, termed the ductal plate, surrounding the portal vein, which eventually remodels into the branching tubular network of bile ducts. The process of bile duct morp...

journal_title：Molecular biology of the cell

authors： Tanimizu N,Miyajima A,Mostov KE

更新日期：2009-05-01 00:00:00

abstract：:Cell morphogenesis is a complex process that relies on a diverse array of proteins and pathways. We have identified a transglutaminase-like protein (Cyk3p) that functions in fission yeast morphogenesis. The phenotype of a cyk3 knockout strain indicates a primary role for Cyk3p in cytokinesis. Correspondingly, Cyk3p lo...

journal_title：Molecular biology of the cell

authors： Pollard LW,Onishi M,Pringle JR,Lord M

更新日期：2012-07-01 00:00:00

abstract：:Glycerophosphoinositol 4-phosphate (GroPIns-4P) is a biologically active, water-soluble phospholipase A metabolite derived from phosphatidylinositol 4-phosphate, whose cellular concentrations have been reported to increase in Ras-transformed cells. It is therefore important to understand its biological activities. Her...

journal_title：Molecular biology of the cell

authors： Mancini R,Piccolo E,Mariggio' S,Filippi BM,Iurisci C,Pertile P,Berrie CP,Corda D

更新日期：2003-02-01 00:00:00

abstract：:Yeast that naturally exhaust the glucose from their environment differentiate into three distinct cell types distinguishable by flow cytometry. Among these is a quiescent (Q) population, which is so named because of its uniform but readily reversed G1 arrest, its fortified cell walls, heat tolerance, and longevity. Da...

journal_title：Molecular biology of the cell

authors： Li L,Miles S,Melville Z,Prasad A,Bradley G,Breeden LL

更新日期：2013-12-01 00:00:00

abstract：:Two pore-domain potassium (K2P) channels mediate potassium background currents that stabilize the resting membrane potential and facilitate action potential repolarization. In the human heart, hK2P17.1 channels are predominantly expressed in the atria and Purkinje cells. Reduced atrial hK2P17.1 protein levels were des...

journal_title：Molecular biology of the cell

authors： Wiedmann F,Schlund D,Voigt N,Ratte A,Kraft M,Katus HA,Schmidt C

更新日期：2019-06-01 00:00:00

abstract：:Myosin II-dependent contraction of the contractile ring drives equatorial furrowing during cytokinesis in animal cells. Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces. Here, we show that in the presence of...

journal_title：Molecular biology of the cell

authors： Kanada M,Nagasaki A,Uyeda TQ

更新日期：2005-08-01 00:00:00

abstract：:Integrin alpha 3A cytoplasmic tail phosphorylation was mapped to amino acid S1042, as determined by mass spectrometry, and confirmed by mutagenesis. This residue occurs within a "QPSXXE" motif conserved in multiple alpha chains (alpha 3A, alpha 6A, alpha 7A), from multiple species. Phosphorylation of alpha 3A and alph...

journal_title：Molecular biology of the cell

authors： Zhang XA,Bontrager AL,Stipp CS,Kraeft SK,Bazzoni G,Chen LB,Hemler ME

更新日期：2001-02-01 00:00:00

abstract：:Insulin maintains whole body blood glucose homeostasis, in part, by regulating the amount of the GLUT4 glucose transporter on the cell surface of fat and muscle cells. Insulin induces the redistribution of GLUT4 from intracellular compartments to the plasma membrane, by stimulating a large increase in exocytosis and a...

journal_title：Molecular biology of the cell

authors： Zeigerer A,McBrayer MK,McGraw TE

更新日期：2004-10-01 00:00:00

abstract：:p38 alpha mitogen-activated protein (MAP) kinase is a broadly expressed signaling molecule that participates in the regulation of cellular responses to stress as well as in the control of proliferation and survival of many cell types. We have used cell lines derived from p38 alpha knockout mice to study the role of th...

journal_title：Molecular biology of the cell

authors： Porras A,Zuluaga S,Black E,Valladares A,Alvarez AM,Ambrosino C,Benito M,Nebreda AR

更新日期：2004-02-01 00:00:00

abstract：:Janus kinase 2 (Jak2) protein tyrosine kinase plays an important role in interleukin-3- or granulocyte-macrophage colony-stimulating factor-mediated signal transduction pathways leading to cell proliferation, activation of early response genes, and inhibition of apoptosis. However, it is unclear whether Jak2 can activ...

journal_title：Molecular biology of the cell

authors： Mohi MG,Arai Ki,Watanabe S

更新日期：1998-12-01 00:00:00

abstract：:The Rab11 effector Rab11-family interacting protein 2 (Rab11-FIP2) regulates transcytosis through its interactions with Rab11a and myosin Vb. Previous studies implicated Rab11-FIP2 in the establishment of polarity in Madin-Darby canine kidney (MDCK) cells through phosphorylation of Ser-227 by MARK2. Here we examine th...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Avant KM,Caldwell CM,Oztan A,Apodaca G,Knowles BC,Roland JT,Ducharme NA,Goldenring JR

更新日期：2012-06-01 00:00:00

abstract：:The polysomal ribonuclease 1 (PMR1) mRNA endonuclease forms a selective complex with its translating substrate mRNAs where it is activated to initiate mRNA decay. Previous work showed tyrosine phosphorylation is required for PMR1 targeting to this polysome-bound complex, and it identified c-Src as the responsible kina...

journal_title：Molecular biology of the cell

authors： Peng Y,Liu X,Schoenberg DR

更新日期：2008-02-01 00:00:00

abstract：:We report a new nuclear gene, designated ATP25 (reading frame YMR098C on chromosome XIII), required for expression of Atp9p (subunit 9) of the Saccharomyces cerevisiae mitochondrial proton translocating ATPase. Mutations in ATP25 elicit a deficit of ATP9 mRNA and of its translation product, thereby preventing assembly...

journal_title：Molecular biology of the cell

authors： Zeng X,Barros MH,Shulman T,Tzagoloff A

更新日期：2008-04-01 00:00:00