Activation and functional analysis of Janus kinase 2 in BA/F3 cells using the coumermycin/gyrase B system.

abstract：:In mammals, a finite population of oocytes is generated during embryogenesis, and proper oocyte meiotic divisions are crucial for fertility. Sperm-associated antigen 1 (SPAG-1) has been implicated in infertility and tumorigenesis; however, its relevance in cell cycle programs remains rudimentary. Here we explore a nov...

journal_title：Molecular biology of the cell

authors： Huang C,Wu D,Khan FA,Jiao X,Guan K,Huo L

更新日期：2016-06-01 00:00:00

abstract：:Numb has been implicated in cortical neurogenesis during nervous system development, as a result of its asymmetric partitioning and antagonizing Notch signaling. Recent studies have revealed that Numb functions in clathrin-dependent endocytosis by binding to the AP-2 complex. Numb is also expressed in postmitotic neur...

journal_title：Molecular biology of the cell

authors： Nishimura T,Yamaguchi T,Tokunaga A,Hara A,Hamaguchi T,Kato K,Iwamatsu A,Okano H,Kaibuchi K

更新日期：2006-03-01 00:00:00

abstract：:Cystic fibrosis (CF) is a fatal genetic disorder associated with defective hydration of lung airways due to the loss of chloride transport through the CF transmembrane conductance regulator protein (CFTR). CFTR contains two membrane-spanning domains (MSDs), two nucleotide-binding domains (NBDs), and a regulatory domai...

journal_title：Molecular biology of the cell

authors： Ren HY,Grove DE,De La Rosa O,Houck SA,Sopha P,Van Goor F,Hoffman BJ,Cyr DM

更新日期：2013-10-01 00:00:00

abstract：:Kinetochores are the proteinaceous complexes that assemble on centromeric DNA and direct eukaryotic chromosome segregation. The mechanisms by which higher eukaryotic cells define centromeres are poorly understood. Possible molecular contributors to centromere specification include the underlying DNA sequences and epig...

journal_title：Molecular biology of the cell

authors： Edwards NS,Murray AW

更新日期：2005-04-01 00:00:00

abstract：:Chironomus tentans-repressor splicing factor (Ct-RSF) represses the activation of splicing by SR proteins in vitro. Ct-RSF colocalizes with the Ser-Arg-rich (SR) protein hrp45 in interchromatin granule clusters and coimmunoprecipitates with hrp45 in nuclear extracts. Ct-RSF and hrp45 can also interact directly in vitr...

journal_title：Molecular biology of the cell

authors： Björk P,Wetterberg-Strandh I,Baurén G,Wieslander L

更新日期：2006-01-01 00:00:00

abstract：:ORP1L is a member of the human oxysterol-binding protein (OSBP) family. ORP1L localizes to late endosomes (LEs)/lysosomes, colocalizing with the GTPases Rab7 and Rab9 and lysosome-associated membrane protein-1. We demonstrate that ORP1L interacts physically with Rab7, preferentially with its GTP-bound form, and provid...

journal_title：Molecular biology of the cell

authors： Johansson M,Lehto M,Tanhuanpää K,Cover TL,Olkkonen VM

更新日期：2005-12-01 00:00:00

abstract：:Lamellipodia of crawling cells represent both the motor for cell advance and the primary building site for the actin cytoskeleton. The organization of actin in the lamellipodium reflects actin dynamics and is of critical importance for the mechanism of cell motility. In previous structural studies, the lamellipodial a...

journal_title：Molecular biology of the cell

authors： Verkhovsky AB,Chaga OY,Schaub S,Svitkina TM,Meister JJ,Borisy GG

更新日期：2003-11-01 00:00:00

abstract：:Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using po...

journal_title：Molecular biology of the cell

authors： Dutcher SK,Morrissette NS,Preble AM,Rackley C,Stanga J

更新日期：2002-11-01 00:00:00

abstract：:The stem-loop binding protein (SLBP1) binds the 3' stem-loop of histone pre-mRNA and is required for efficient processing of histone transcripts in the nucleus. We examined the localization of SLBP1 in the germinal vesicle of Xenopus laevis oocytes. In spread preparations of germinal vesicle contents, an anti-SLBP1 an...

journal_title：Molecular biology of the cell

authors： Abbott J,Marzluff WF,Gall JG

更新日期：1999-02-01 00:00:00

abstract：:The expression of FREQUENCY, a central component of the circadian clock in Neurospora crassa, shows daily cycles that are exquisitely sensitive to the environment. Two forms of FRQ that differ in length by 99 amino acids, LFRQ and SFRQ, are synthesized from alternative initiation codons and the change in their ratio a...

journal_title：Molecular biology of the cell

authors： Colot HV,Loros JJ,Dunlap JC

更新日期：2005-12-01 00:00:00

abstract：:The ubiquitin-like hPLIC proteins can associate with proteasomes, and hPLIC overexpression can specifically interfere with ubiquitin-mediated proteolysis (Kleijnen et al., 2000). Because the hPLIC proteins can also interact with certain E3 ubiquitin protein ligases, they may provide a link between the ubiquitination a...

journal_title：Molecular biology of the cell

authors： Kleijnen MF,Alarcon RM,Howley PM

更新日期：2003-09-01 00:00:00

abstract：:The neonatal receptor for immunoglobulin G (IgG; FcRn) prevents IgG degradation by efficiently sorting IgG into recycling endosomes and away from lysosomes. When bound to IgG-opsonized antigen complexes, however, FcRn traffics cargo into lysosomes, where antigen processing can occur. Here we address the mechanism of s...

journal_title：Molecular biology of the cell

authors： Weflen AW,Baier N,Tang QJ,Van den Hof M,Blumberg RS,Lencer WI,Massol RH

更新日期：2013-08-01 00:00:00

abstract：:Apoptosis causes characteristic morphological changes in cells, including membrane blebbing, cell detachment from the extracellular matrix, and loss of cell-cell contacts. We investigated the changes in focal adhesion proteins during etoposide-induced apoptosis in Rat-1 cells and found that during apoptosis, p130cas (...

journal_title：Molecular biology of the cell

authors： Kook S,Shim SR,Choi SJ,Ahnn J,Kim JI,Eom SH,Jung YK,Paik SG,Song WK

更新日期：2000-03-01 00:00:00

abstract：:Significant increase in JNK, c-Jun, and Cdk5 activities are reported in Alzheimer's disease (AD). Inhibition of c-Jun prevents neuronal cell death in in vivo AD models, highlighting it as a major JNK effector. Both JNK and Cdk5 promote neurodegeneration upon deregulation; however, Cdk5 has not been mechanistically lin...

journal_title：Molecular biology of the cell

authors： Sun KH,Lee HG,Smith MA,Shah K

更新日期：2009-11-01 00:00:00

abstract：:Biochemical data have shown that COPI-coated vesicles are tethered to Golgi membranes by a complex of at least three proteins: p115, giantin, and GM130. p115 binds to giantin on the vesicles and to GM130 on the membrane. We now examine the function of this tethering complex in vivo. Microinjection of an N-terminal pep...

journal_title：Molecular biology of the cell

authors： Seemann J,Jokitalo EJ,Warren G

更新日期：2000-02-01 00:00:00

abstract：:To test the "traction fiber" model for metaphase positioning of bivalents during meiosis, kinetochore fibers of maloriented bivalents, induced during recovery from cold arrest, were analyzed with a liquid crystal polarizing microscope. The measured birefringence retardation of kinetochore fibers is proportional to the...

journal_title：Molecular biology of the cell

authors： LaFountain JR Jr,Oldenbourg R

更新日期：2004-12-01 00:00:00

abstract：:Pituitary adenylate cyclase-activating polypeptide (PACAP) is a potent neuropeptide that possesses both neurotrophic and neurodevelopmental effects. Recently, the Rit GTPase was found to be activated by a novel Galpha/cAMP/exchange protein activated by cyclic AMP (Epac)-dependent signaling pathway and required for PAC...

journal_title：Molecular biology of the cell

authors： Shi GX,Jin L,Andres DA

更新日期：2010-05-01 00:00:00

abstract：:Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor-induced ...

journal_title：Molecular biology of the cell

authors： Cortesio CL,Perrin BJ,Bennin DA,Huttenlocher A

更新日期：2010-01-01 00:00:00

abstract：:LLCPK-1 cells were transfected with a green fluorescent protein (GFP)-alpha tubulin construct and a cell line permanently expressing GFP-alpha tubulin was established (LLCPK-1alpha). The mitotic index and doubling time for LLCPK-1alpha were not significantly different from parental cells. Quantitative immunoblotting s...

journal_title：Molecular biology of the cell

authors： Rusan NM,Fagerstrom CJ,Yvon AM,Wadsworth P

更新日期：2001-04-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef directs virus escape from immune surveillance by subverting host cell intracellular signaling and membrane traffic to down-regulate cell-surface major histocompatibility complex class I (MHC-I). The interaction of Nef with the sorting proteins PACS-...

journal_title：Molecular biology of the cell

authors： Dikeakos JD,Thomas L,Kwon G,Elferich J,Shinde U,Thomas G

更新日期：2012-06-01 00:00:00

abstract：:During oogenesis, two successive meiotic cell divisions occur without functional centrosomes because of the inactivation and subsequent elimination of maternal centrosomes during the diplotene stage of meiosis I. Despite being a conserved phenomenon in most metazoans, the means by which this centrosome behavior is con...

journal_title：Molecular biology of the cell

authors： Matsuura R,Ashikawa T,Nozaki Y,Kitagawa D

更新日期：2016-03-01 00:00:00

abstract：:The TAM receptor protein tyrosine kinases Tyro3, Axl, and Mer play important roles in macrophage function. We investigated the roles of the TAM receptors in mediating the induction of hepatocyte growth factor (HGF) during the interaction of macrophages with apoptotic cells. Mer-specific neutralizing antibody, small in...

journal_title：Molecular biology of the cell

authors： Park HJ,Baen JY,Lee YJ,Choi YH,Kang JL

更新日期：2012-08-01 00:00:00

abstract：:Tubulogenesis by epithelial cells regulates kidney, lung, and mammary development, whereas that by endothelial cells regulates vascular development. Although functionally dissimilar, the processes necessary for tubulation by epithelial and endothelial cells are very similar. We performed microarray analysis to further...

journal_title：Molecular biology of the cell

authors： Albig AR,Schiemann WP

更新日期：2005-02-01 00:00:00

abstract：:CWH41, a gene involved in the assembly of cell wall beta-1,6-glucan, has recently been shown to be the structural gene for Saccharomyces cerevisiae glucosidase I that is responsible for initiating the trimming of terminal alpha-1,2-glucose residue in the N-glycan processing pathway. To distinguish between a direct or ...

journal_title：Molecular biology of the cell

authors： Abeijon C,Chen LY

更新日期：1998-10-01 00:00:00

abstract：:The c-myb proto-oncogene product (c-Myb) regulates proliferation and differentiation of hematopoietic cells. Recently we have shown that c-Myb is degraded in response to Wnt-1 stimulation via a pathway involving TAK1 (TGF-beta-activated kinase), HIPK2 (homeodomain-interacting protein kinase 2), and NLK (Nemo-like kina...

journal_title：Molecular biology of the cell

authors： Kurahashi T,Nomura T,Kanei-Ishii C,Shinkai Y,Ishii S

更新日期：2005-10-01 00:00:00

abstract：:Two different human diseases, X-linked myotubular myopathy and Charcot-Marie-Tooth disease, result from mutant MTM1 or MTMR2 lipid phosphatases. Although events involved in endosomal PI(3)P and PI(3,5)P(2) synthesis are well established and pivotal in receptor signaling and degradation, enzymes involved in phosphoinos...

journal_title：Molecular biology of the cell

authors： Cao C,Backer JM,Laporte J,Bedrick EJ,Wandinger-Ness A

更新日期：2008-08-01 00:00:00

abstract：:Maturation-promoting factor, consisting of cdc2 protein kinase and a regulatory B-type cyclin, is a universal regulator of meiosis and mitosis in eukaryotes. In Xenopus, there are two subtypes of B-type cyclins, designated B1 and B2, both of which are phosphorylated. In this study, we have investigated the biological ...

journal_title：Molecular biology of the cell

authors： Li J,Meyer AN,Donoghue DJ

更新日期：1995-09-01 00:00:00

abstract：:Ubiquitinated integral membrane proteins are delivered to the interior of the lysosome/vacuole for degradation. This process relies on specific ubiquitination of potential cargo and recognition of that Ub-cargo by sorting receptors at multiple compartments. We show that the endosomal Hse1-Vps27 sorting receptor binds ...

journal_title：Molecular biology of the cell

authors： Ren J,Kee Y,Huibregtse JM,Piper RC

更新日期：2007-01-01 00:00:00

abstract：:Tolerance to desiccation in cultures of Saccharomyces cerevisiae is inducible; only one in a million cells from an exponential culture survive desiccation compared with one in five cells in stationary phase. Here we exploit the desiccation sensitivity of exponentially dividing cells to understand the stresses imposed ...

journal_title：Molecular biology of the cell

authors： Welch AZ,Gibney PA,Botstein D,Koshland DE

更新日期：2013-01-01 00:00:00

abstract：:The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail ...

journal_title：Molecular biology of the cell

authors： Pollard TD

更新日期：2010-12-01 00:00:00