Abstract:
:Soluble N-ethyl maleimide-sensitive fusion protein attachment protein receptors (SNAREs) are core machinery for membrane fusion during intracellular vesicular transport. Synaptosome-associated protein of 23 kDa (SNAP23) is a target SNARE previously identified at the plasma membrane, where it is involved in exocytotic membrane fusion. Here we show that SNAP23 associates with vimentin filaments in a Triton X-100 insoluble fraction in fibroblasts in primary culture and HeLa cells. Upon treatment of human fibroblasts with N-ethyl-maleimide, SNAP23 dissociates from vimentin filaments and forms a protein complex with syntaxin 4, a plasma membrane SNARE. The vimentin-associated pool of SNAP23 can therefore be a reservoir, which would supply the plasma membrane fusion machinery, in fibroblasts. Our observation points to a yet unexplored role of intermediate filaments.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Faigle W,Colucci-Guyon E,Louvard D,Amigorena S,Galli Tdoi
10.1091/mbc.11.10.3485keywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
3485-94issue
10eissn
1059-1524issn
1939-4586journal_volume
11pub_type
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