Selective protein exit from yeast endoplasmic reticulum in absence of functional COPII coat component Sec13p.

abstract：:The β4 isoform of the β-subunits of voltage-gated calcium channel regulates cell proliferation and cell cycle progression. Herein we show that coexpression of the β4-subunit with actors of the canonical Wnt/β-catenin signaling pathway in a hepatoma cell line inhibits Wnt-responsive gene transcription and decreases cel...

journal_title：Molecular biology of the cell

authors： Rima M,Daghsni M,Lopez A,Fajloun Z,Lefrancois L,Dunach M,Mori Y,Merle P,Brusés JL,De Waard M,Ronjat M

更新日期：2017-12-01 00:00:00

abstract：:In mammals, a finite population of oocytes is generated during embryogenesis, and proper oocyte meiotic divisions are crucial for fertility. Sperm-associated antigen 1 (SPAG-1) has been implicated in infertility and tumorigenesis; however, its relevance in cell cycle programs remains rudimentary. Here we explore a nov...

journal_title：Molecular biology of the cell

authors： Huang C,Wu D,Khan FA,Jiao X,Guan K,Huo L

更新日期：2016-06-01 00:00:00

abstract：:Damaged DNA-binding protein 2 (DDB2), a nuclear protein, participates in both nucleotide excision repair and mRNA transcription. The transcriptional regulatory function of DDB2 is significant in colon cancer, as it regulates metastasis. To characterize the mechanism by which DDB2 participates in transcription, we inve...

journal_title：Molecular biology of the cell

authors： Fantini D,Huang S,Asara JM,Bagchi S,Raychaudhuri P

更新日期：2017-01-01 00:00:00

abstract：:Integrins are major receptors used by cells to interact with extracellular matrices. In this paper, we identify the first ligands for the beta 8 family of integrins, presenting evidence that integrin heterodimers containing the beta 8 subunit mediate interactions of chick sensory neurons with laminin-1, collagen IV, a...

journal_title：Molecular biology of the cell

authors： Venstrom K,Reichardt L

更新日期：1995-04-01 00:00:00

abstract：:The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail ...

journal_title：Molecular biology of the cell

authors： Pollard TD

更新日期：2010-12-01 00:00:00

abstract：:Surface charges at the inner leaflet of the plasma membrane may contribute to regulate the surface recruitment of key signaling factors. Phosphatidylserine (PS) is an abundant charged lipid that may regulate charge distribution in different cell types. Here we characterize the subcellular distribution and function of ...

journal_title：Molecular biology of the cell

authors： Haupt A,Minc N

更新日期：2017-01-01 00:00:00

abstract：:Myc interacting zinc finger protein-1 (Miz1) is a transcription factor known to regulate cell cycle- and cell adhesion-related genes in cancer. Here we show that Miz1 also plays a critical role in neural crest development. In the chick, Miz1 is expressed throughout the neural plate and closing neural tube. Its morphol...

journal_title：Molecular biology of the cell

authors： Kerosuo L,Bronner ME

更新日期：2014-02-01 00:00:00

abstract：:During stem cell divisions, mitotic microtubules do more than just segregate the chromosomes. They also determine whether a cell divides virtually symmetrically or asymmetrically by establishing spindle orientation and the plane of cell division. This can be decisive for the fate of the stem cell progeny. Spindle defe...

journal_title：Molecular biology of the cell

authors： Mora-Bermúdez F,Huttner WB

更新日期：2015-12-01 00:00:00

abstract：:Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synaps...

journal_title：Molecular biology of the cell

authors： Mentlik AN,Sanborn KB,Holzbaur EL,Orange JS

更新日期：2010-07-01 00:00:00

abstract：:A morphogenesis checkpoint in budding yeast delays nuclear division (and subsequent cell cycle progression) in cells that have failed to make a bud. We show that the ability of this checkpoint to delay nuclear division requires the SWE1 gene, encoding a protein kinase that inhibits the master cell cycle regulatory kin...

journal_title：Molecular biology of the cell

authors： Sia RA,Herald HA,Lew DJ

更新日期：1996-11-01 00:00:00

abstract：:Microtubules (MTs) have been proposed to act mechanically as compressive struts that resist both actomyosin contractile forces and their own polymerization forces to mechanically stabilize cell shape. To identify the origin of MT bending, we directly observed MT bending and F-actin transport dynamics in the periphery ...

journal_title：Molecular biology of the cell

authors： Bicek AD,Tüzel E,Demtchouk A,Uppalapati M,Hancock WO,Kroll DM,Odde DJ

更新日期：2009-06-01 00:00:00

abstract：:Dopamine (DA) increases Na(+),K(+)-ATPase activity in lung alveolar epithelial cells. This effect is associated with an increase in Na(+),K(+)-ATPase molecules within the plasma membrane (). Analysis of Na(+),K(+)-ATPase motion was performed in real-time in alveolar cells stably expressing Na(+),K(+)-ATPase molecules ...

journal_title：Molecular biology of the cell

authors： Bertorello AM,Komarova Y,Smith K,Leibiger IB,Efendiev R,Pedemonte CH,Borisy G,Sznajder JI

更新日期：2003-03-01 00:00:00

abstract：:The conserved NDR-kinase Dbf2 plays a critical role in cytokinesis in budding yeast. Among its cytokinesis-related substrates is the F-BAR protein Hof1. Hof1 colocalizes at the cell division site with the septin complex and, as mitotic exit progresses, moves to the actomyosin ring (AMR). Neither the function of Hof1 a...

journal_title：Molecular biology of the cell

authors： Meitinger F,Palani S,Hub B,Pereira G

更新日期：2013-05-01 00:00:00

abstract：:Previous work has established that activation of Mos, Mek, and p42 mitogen-activated protein (MAP) kinase can trigger release from G2-phase arrest in Xenopus oocytes and oocyte extracts and can cause Xenopus embryos and extracts to arrest in mitosis. Herein we have found that activation of the MAP kinase cascade can a...

journal_title：Molecular biology of the cell

authors： Walter SA,Guadagno TM,Ferrell JE Jr

更新日期：1997-11-01 00:00:00

abstract：:Isu, the scaffold protein on which Fe-S clusters are built in the mitochondrial matrix, plays a central role in the biogenesis of Fe-S cluster proteins. We report that the reduction in the activity of several components of the cluster biogenesis system, including the specialized Hsp70 Ssq1, causes a 15-20-fold up-regu...

journal_title：Molecular biology of the cell

authors： Andrew AJ,Song JY,Schilke B,Craig EA

更新日期：2008-12-01 00:00:00

abstract：:The yeast DASH complex is a heterodecameric component of the kinetochore necessary for accurate chromosome segregation. DASH forms closed rings around microtubules with a large gap between the DASH ring and the microtubule cylinder. We characterized the microtubule-binding properties of limited proteolysis products an...

journal_title：Molecular biology of the cell

authors： Miranda JJ,King DS,Harrison SC

更新日期：2007-07-01 00:00:00

abstract：:We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the horm...

journal_title：Molecular biology of the cell

authors： McIsaac RS,Silverman SJ,McClean MN,Gibney PA,Macinskas J,Hickman MJ,Petti AA,Botstein D

更新日期：2011-11-01 00:00:00

abstract：:Tetraploidization, or genome doubling, is a prominent event in tumorigenesis, primarily because cell division in polyploid cells is error-prone and produces aneuploid cells. This study investigates changes in gene expression evoked in acute and adapted tetraploid cells and their effect on cell-cycle progression. Acute...

journal_title：Molecular biology of the cell

authors： Potapova TA,Seidel CW,Box AC,Rancati G,Li R

更新日期：2016-10-15 00:00:00

abstract：:Formation and discharge of dense-core secretory vesicles depend on controlled rearrangement of the core proteins during their assembly and dispersal. The ciliate Tetrahymena thermophila offers a simple system in which the mechanisms may be studied. Here we show that most of the core consists of a set of polypeptides d...

journal_title：Molecular biology of the cell

authors： Verbsky JW,Turkewitz AP

更新日期：1998-02-01 00:00:00

abstract：:Lipoprotein(a) (Lp(a)) is associated with cardiovascular disease risk. This may be attributable to the ability of Lp(a) to elicit endothelial dysfunction. We previously reported that apolipoprotein(a) (apo(a); the distinguishing kringle-containing component of Lp(a)) elicits cytoskeletal rearrangements in vascular end...

journal_title：Molecular biology of the cell

authors： Cho T,Romagnuolo R,Scipione C,Boffa MB,Koschinsky ML

更新日期：2013-02-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are extracellular proteases highly expressed at wound sites. However, the precise function of MMPs during reepithelialization in vivo has been elusive in mammalian models because of the high level of redundancy among the 24 mammalian MMPs. For this reason we used Drosophila melanogaste...

journal_title：Molecular biology of the cell

authors： Stevens LJ,Page-McCaw A

更新日期：2012-03-01 00:00:00

abstract：:Mammalian target of rapamycin (mTOR) forms two complexes, mTORC1 and mTORC2, that play central roles in cell growth and functions. Only mTORC1 is directly inhibited by the immunosuppressive drug rapamycin. Despite recent progress in identifying new components and functions of the mTOR pathway, relatively little is kno...

journal_title：Molecular biology of the cell

authors： Liu X,Zheng XF

更新日期：2007-03-01 00:00:00

abstract：:McKusick-Kaufman syndrome (MKKS) is a recessively inherited human genetic disease characterized by several developmental anomalies. Mutations in the MKKS gene also cause Bardet-Biedl syndrome (BBS), a genetically heterogeneous disorder with pleiotropic symptoms. However, little is known about how MKKS mutations lead t...

journal_title：Molecular biology of the cell

authors： Hirayama S,Yamazaki Y,Kitamura A,Oda Y,Morito D,Okawa K,Kimura H,Cyr DM,Kubota H,Nagata K

更新日期：2008-03-01 00:00:00

abstract：:Timely down-regulation of the evolutionarily conserved protein kinase Swe1 plays an important role in cell cycle control, as Swe1 can block nuclear division through inhibitory phosphorylation of the catalytic subunit of cyclin-dependent kinase. In particular, Swe1 degradation is important for budding yeast cell surviv...

journal_title：Molecular biology of the cell

authors： Raspelli E,Cassani C,Lucchini G,Fraschini R

更新日期：2011-07-01 00:00:00

abstract：:The gene encoding NFKB1 is autoregulated, responding to NF-kappa B/Rel activation through NF-kappa B binding sites in its promoter, which also contains putative sites for Ets proteins. One of the Ets sites, which we refer to as EBS4, is located next to an NF-kappa B/Rel binding site, kB3, which is absolutely required ...

journal_title：Molecular biology of the cell

authors： Lambert PF,Ludford-Menting MJ,Deacon NJ,Kola I,Doherty RR

更新日期：1997-02-01 00:00:00

abstract：:The TOR kinases are conserved negative regulators of autophagy in response to nutrient conditions, but the signaling mechanisms are poorly understood. Here we describe a complex containing the protein kinase Atg1 and the phosphoprotein Atg13 that functions as a critical component of this regulation in Drosophila. We s...

journal_title：Molecular biology of the cell

authors： Chang YY,Neufeld TP

更新日期：2009-04-01 00:00:00

abstract：:Schizosaccharomyces pombe Rho GTPases regulate actin cytoskeleton organization and cell integrity. We studied the fission yeast gene SPBC4F6.12 based on its ability to suppress the thermosensitivity of cdc42-1625 mutant strain. This gene, named pxl1(+), encodes a protein with three LIM domains that is similar to paxil...

journal_title：Molecular biology of the cell

authors： Pinar M,Coll PM,Rincón SA,Pérez P

更新日期：2008-04-01 00:00:00

abstract：:The Cdc42 guanosine triphosphatase (GTPase) plays a central role in polarity development in species ranging from yeast to humans. In budding yeast, a specific growth site is selected in the G1 phase. Rsr1, a Ras GTPase, interacts with Cdc42 and its associated proteins to promote polarized growth at the proper bud site...

journal_title：Molecular biology of the cell

authors： Miller KE,Lo WC,Chou CS,Park HO

更新日期：2019-09-15 00:00:00

abstract：:Toll-like receptor 4 interactor with leucine-rich repeats (Tril) functions as a coreceptor for Toll-like receptors (Tlrs) to mediate innate immune responses in adults. In embryos, Tril signals to promote degradation of the Bmp inhibitor, Smad7, to allow for blood formation. It is not known whether this function requir...

journal_title：Molecular biology of the cell

authors： Kim HS,McKnite A,Xie Y,Christian JL

更新日期：2018-03-01 00:00:00

abstract：:The PDZ domain-containing protein CAL mediates lysosomal trafficking and degradation of CFTR. Here we demonstrate the involvement of a CAL-binding SNARE protein syntaxin 6 (STX6) in this process. Overexpression of STX6, which colocalizes and coimmunoprecipitates with CAL, dramatically reduces the steady-state level an...

journal_title：Molecular biology of the cell

authors： Cheng J,Cebotaru V,Cebotaru L,Guggino WB

更新日期：2010-04-01 00:00:00