The GTPase SPAG-1 orchestrates meiotic program by dictating meiotic resumption and cytoskeleton architecture in mouse oocytes.

abstract：:Trends in increased tuberculosis infection and a fatality rate of approximately 23% have necessitated the search for alternative biomarkers using newly developed postgenomic approaches. Here we provide a systematic analysis of Mycobacterium tuberculosis (Mtb) by directly profiling its gene products. This analysis comb...

journal_title：Molecular biology of the cell

authors： Mawuenyega KG,Forst CV,Dobos KM,Belisle JT,Chen J,Bradbury EM,Bradbury AR,Chen X

更新日期：2005-01-01 00:00:00

abstract：:Despite the fact that the chromosomal passenger complex is well known to regulate kinetochore behavior in mitosis, no functional link has yet been established between the complex and kinetochore structure. In addition, remarkably little is known about how the complex targets to centromeres. Here, in a study of caspase...

journal_title：Molecular biology of the cell

authors： Faragher AJ,Sun XM,Butterworth M,Harper N,Mulheran M,Ruchaud S,Earnshaw WC,Cohen GM

更新日期：2007-04-01 00:00:00

abstract：:All vertebrates contain two nonmuscle myosin II heavy chains, A and B, which differ in tissue expression and subcellular distributions. To understand how these distinct distributions are controlled and what role they play in cell migration, myosin IIA and IIB were examined during wound healing by bovine aortic endothe...

journal_title：Molecular biology of the cell

authors： Kolega J

更新日期：2003-12-01 00:00:00

abstract：:Satellite cells (SCs), the resident adult stem cells of skeletal muscle, are required for tissue repair throughout life. While many signaling pathways are known to control SC self-renewal, less is known about the mechanisms underlying the spatiotemporal control of self-renewal during skeletal muscle repair. Here, we m...

journal_title：Molecular biology of the cell

authors： Moyle LA,Cheng RY,Liu H,Davoudi S,Ferreira SA,Nissar AA,Sun Y,Gentleman E,Simmons CA,Gilbert PM

更新日期：2020-07-21 00:00:00

abstract：:Normal epithelial cells undergo apoptosis when they are denied contact with the extracellular matrix, in a process termed "anoikis." Conversely, malignant epithelial cells typically acquire anchorage independence, i.e., the capacity to survive and grow in the absence of matrix interaction. Here we asked the question w...

journal_title：Molecular biology of the cell

authors： Jost M,Huggett TM,Kari C,Rodeck U

更新日期：2001-05-01 00:00:00

abstract：:MPM-2 antigens, a discrete set of phosphoproteins that contain similar phosphoepitopes recognized by the monoclonal antibody MPM-2, are phosphorylated during M-phase induction. Our previous studies suggested that certain MPM-2 antigens are involved in the appearance of maturation-promoting factor (MPF) activity. Becau...

journal_title：Molecular biology of the cell

authors： Kuang J,Ashorn CL,Gonzalez-Kuyvenhoven M,Penkala JE

更新日期：1994-02-01 00:00:00

abstract：:We have identified and characterized sorb-1, the only sorbin and SH3 domain-containing protein family member in Caenorhabditis elegans SORB-1 is strongly localized to integrin adhesion complexes in larvae and adults, including adhesion plaques and dense bodies (Z-disks) of striated muscles and attachment plaques of sm...

journal_title：Molecular biology of the cell

authors： Loveless T,Qadota H,Benian GM,Hardin J

更新日期：2017-12-01 00:00:00

abstract：:Desmin interacts with nebulin establishing a direct link between the intermediate filament network and sarcomeres at the Z-discs. Here, we examined a desmin mutation, E245D, that is located within the coil IB (nebulin-binding) region of desmin and that has been reported to cause human cardiomyopathy and skeletal muscl...

journal_title：Molecular biology of the cell

authors： Conover GM,Henderson SN,Gregorio CC

更新日期：2009-02-01 00:00:00

abstract：:The actin cytoskeleton is constantly assembling and disassembling. Cells harness the energy of these turnover dynamics to drive cell motility and organize cytoplasm. Although much is known about how cells control actin polymerization, we do not understand how actin filaments depolymerize inside cells. I briefly descri...

journal_title：Molecular biology of the cell

authors： Brieher W

更新日期：2013-08-01 00:00:00

abstract：:We have shown previously that interleukin-4 (IL-4) protects TS1alphabeta cells from apoptosis, but very little is known about the mechanism by which IL-4 exerts this effect. We found that Akt activity, which is dependent on phosphatidylinositol 3 kinase, is reduced in IL-4-deprived TS1alphabeta cells. Overexpression o...

journal_title：Molecular biology of the cell

authors： Cerezo A,Martínez-A C,Lanzarot D,Fischer S,Franke TF,Rebollo A

更新日期：1998-11-01 00:00:00

abstract：:The spindle assembly checkpoint (SAC) is an evolutionarily conserved surveillance mechanism that delays anaphase onset and mitotic exit in response to the lack of kinetochore attachment. The target of the SAC is the E3 ubiquitin ligase anaphase-promoting complex (APC) bound to its Cdc20 activator. The Cdc20/APC comple...

journal_title：Molecular biology of the cell

authors： Chiroli E,Rancati G,Catusi I,Lucchini G,Piatti S

更新日期：2009-05-01 00:00:00

abstract：:The mitotic exit network (MEN) is an essential GTPase signaling pathway that triggers exit from mitosis in budding yeast. We show here that during meiosis, the MEN is dispensable for exit from meiosis I but contributes to the timely exit from meiosis II. Consistent with a role for the MEN during meiosis II, we find th...

journal_title：Molecular biology of the cell

authors： Attner MA,Amon A

更新日期：2012-08-01 00:00:00

abstract：:Epithelial tissues are protective barriers that display a remarkable ability to repair wounds. Wound repair is often associated with an accumulation of actin and nonmuscle myosin II around the wound, forming a purse string. The role of actomyosin networks in generating mechanical force during wound repair is not well ...

journal_title：Molecular biology of the cell

authors： Fernandez-Gonzalez R,Zallen JA

更新日期：2013-10-01 00:00:00

abstract：:To determine how the human cdc25 gene product acts to regulate p34cdc2 at the G2 to M transition, we have overproduced the full-length protein (cdc25Hs) as well as several deletion mutants in bacteria as glutathione-S-transferase fusion proteins. The wild-type cdc25Hs gene product was synthesized as an 80-kDa fusion p...

journal_title：Molecular biology of the cell

authors： Lee MS,Ogg S,Xu M,Parker LL,Donoghue DJ,Maller JL,Piwnica-Worms H

更新日期：1992-01-01 00:00:00

abstract：:Podosomes are dynamic actin-rich structures composed of a dense F-actin core surrounded by a cloud of more diffuse F-actin. Src performs one or more unique functions in osteoclasts (OCLs), and podosome belts and bone resorption are impaired in the absence of Src. Using Src(-/-) OCLs, we investigated the specific funct...

journal_title：Molecular biology of the cell

authors： Destaing O,Sanjay A,Itzstein C,Horne WC,Toomre D,De Camilli P,Baron R

更新日期：2008-01-01 00:00:00

abstract：:Formins have conserved roles in cell polarity and cytokinesis and directly nucleate actin filament assembly through their FH2 domain. Here, we define the active region of the yeast formin Bni1 FH2 domain and show that it dimerizes. Mutations that disrupt dimerization abolish actin assembly activity, suggesting that di...

journal_title：Molecular biology of the cell

authors： Moseley JB,Sagot I,Manning AL,Xu Y,Eck MJ,Pellman D,Goode BL

更新日期：2004-02-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) is a key regulator of mitotic progression and cell division in eukaryotes. It is highly expressed in tumor cells and considered a potential target for cancer therapy. Here, we report the discovery and application of a novel potent small-molecule inhibitor of mammalian Plk1, ZK-Thiazolidinone ...

journal_title：Molecular biology of the cell

authors： Santamaria A,Neef R,Eberspächer U,Eis K,Husemann M,Mumberg D,Prechtl S,Schulze V,Siemeister G,Wortmann L,Barr FA,Nigg EA

更新日期：2007-10-01 00:00:00

abstract：:Meiotic recombination differs from mitotic recombination in that DSBs are repaired using homologous chromosomes, rather than sister chromatids. This change in partner choice is due in part to a barrier to sister chromatid repair (BSCR) created by the meiosis-specific kinase, Mek1, in a complex with two other meiosis-s...

journal_title：Molecular biology of the cell

authors： Niu H,Wan L,Baumgartner B,Schaefer D,Loidl J,Hollingsworth NM

更新日期：2005-12-01 00:00:00

abstract：:Cancer cell migration requires the regulation of actin networks at protrusions associated with invadopodia and other leading edges. Carcinomas become invasive after undergoing an epithelial-mesenchymal transition characterized by the appearance of vimentin filaments. While vimentin expression correlates with cell migr...

journal_title：Molecular biology of the cell

authors： Lanier MH,Kim T,Cooper JA

更新日期：2015-12-15 00:00:00

abstract：:During oogenesis, two successive meiotic cell divisions occur without functional centrosomes because of the inactivation and subsequent elimination of maternal centrosomes during the diplotene stage of meiosis I. Despite being a conserved phenomenon in most metazoans, the means by which this centrosome behavior is con...

journal_title：Molecular biology of the cell

authors： Matsuura R,Ashikawa T,Nozaki Y,Kitagawa D

更新日期：2016-03-01 00:00:00

abstract：:Macrophage fusion resulting in the formation of multinucleated giant cells occurs in a variety of chronic inflammatory diseases, yet the mechanism responsible for initiating this process is unknown. Here, we used live cell imaging to show that actin-based protrusions at the leading edge initiate macrophage fusion. Pha...

journal_title：Molecular biology of the cell

authors： Faust JJ,Balabiyev A,Heddleston JM,Podolnikova NP,Baluch DP,Chew TL,Ugarova TP

更新日期：2019-08-01 00:00:00

abstract：:Previously, we showed that spliceosomal U6 small nuclear RNA (snRNA) transiently passes through the nucleolus. Herein, we report that all individual snRNAs of the [U4/U6.U5] tri-snRNP localize to nucleoli, demonstrated by fluorescence microscopy of nucleolar preparations after injection of fluorescein-labeled snRNA in...

journal_title：Molecular biology of the cell

authors： Gerbi SA,Lange TS

更新日期：2002-09-01 00:00:00

abstract：:Since 2008, the autophagy community has periodically published a set of guidelines, currently titled "Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy." The newest version of the guidelines was published in 2016. There are many reasons for establishing a set of guidelines in a given researc...

journal_title：Molecular biology of the cell

authors： Klionsky DJ

更新日期：2016-03-01 00:00:00

abstract：:Mitochondrial function depends crucially on the maintenance of multiple mitochondrial DNA (mtDNA) copies. Surprisingly, the cellular mechanisms regulating mtDNA copy number remain poorly understood. Through a systematic high-throughput approach in Saccharomyces cerevisiae, we determined mtDNA-to-nuclear DNA ratios in ...

journal_title：Molecular biology of the cell

authors： Göke A,Schrott S,Mizrak A,Belyy V,Osman C,Walter P

更新日期：2020-03-19 00:00:00

abstract：:We have recently shown that the pancreatic bile salt-dependent lipase (BSDL) can be taken up by intestinal cells and transported to the blood circulation. This mechanism likely involves (specific) receptor(s) able to bind BSDL and located at the apical intestinal cell membrane. In this study, using Int407 human intest...

journal_title：Molecular biology of the cell

authors： Bruneau N,Richard S,Silvy F,Verine A,Lombardo D

更新日期：2003-07-01 00:00:00

abstract：:Sec13p has been thought to be an essential component of the COPII coat, required for exit of proteins from the yeast endoplasmic reticulum (ER). We show herein that normal function of Sec13p was not required for ER exit of the Hsp150 glycoprotein. Hsp150 was secreted to the medium under restrictive conditions in a sec...

journal_title：Molecular biology of the cell

authors： Fatal N,Suntio T,Makarow M

更新日期：2002-12-01 00:00:00

abstract：:During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the Gα12/13 pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understo...

journal_title：Molecular biology of the cell

authors： Xie S,Mason FM,Martin AC

更新日期：2016-11-07 00:00:00

abstract：:In autosomal dominant polycystic kidney disease (ADPKD), cysts accumulate and progressively impair renal function. Mutations in PKD1 and PKD2 genes are causally linked to ADPKD, but how these mutations drive cell behaviors that underlie ADPKD pathogenesis is unknown. Human ADPKD cysts frequently express cadherin-8 (ca...

journal_title：Molecular biology of the cell

authors： Belmonte JM,Clendenon SG,Oliveira GM,Swat MH,Greene EV,Jeyaraman S,Glazier JA,Bacallao RL

更新日期：2016-11-07 00:00:00

abstract：:The Ste5 scaffold activates an associated mitogen-activated protein kinase cascade by binding through its RING-H2 domain to a Gbetagamma dimer (Ste4/Ste18) at the plasma membrane in a recruitment event that requires prior nuclear shuttling of Ste5. Genetic evidence suggests that Ste5 must oligomerize to function, but ...

journal_title：Molecular biology of the cell

authors： Wang Y,Elion EA

更新日期：2003-06-01 00:00:00

abstract：:Chironomus tentans-repressor splicing factor (Ct-RSF) represses the activation of splicing by SR proteins in vitro. Ct-RSF colocalizes with the Ser-Arg-rich (SR) protein hrp45 in interchromatin granule clusters and coimmunoprecipitates with hrp45 in nuclear extracts. Ct-RSF and hrp45 can also interact directly in vitr...

journal_title：Molecular biology of the cell

authors： Björk P,Wetterberg-Strandh I,Baurén G,Wieslander L

更新日期：2006-01-01 00:00:00