Abstract:
:During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the Gα12/13 pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understood. Coupling live-cell imaging with a computational approach to identify contractile events, we discovered that differences in constriction behavior are biased by initial cell shape. Disrupting Gα12/13 exacerbates this relationship. Larger apical area is associated with delayed initiation of contractile pulses, lower apical E-cadherin and F-actin levels, and aberrantly mobile Rho-kinase structures. Our results suggest that loss of Gα12/13 disrupts apical actin cortex organization and pulse initiation in a size-dependent manner. We propose that Gα12/13 robustly organizes the apical cortex despite variation in apical area to ensure the timely initiation of contractile pulses in a tissue with heterogeneity in starting cell shape.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Xie S,Mason FM,Martin ACdoi
10.1091/mbc.E16-05-0305subject
Has Abstractpub_date
2016-11-07 00:00:00pages
3526-3536issue
22eissn
1059-1524issn
1939-4586pii
mbc.E16-05-0305journal_volume
27pub_type
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