The extracellular signal-regulated kinase-mitogen-activated protein kinase pathway phosphorylates and targets Cdc25A for SCF beta-TrCP-dependent degradation for cell cycle arrest.

abstract：:Nuclear migration is required for normal development in both higher and lower eukaryotes. In fungi this process is mediated by cytoplasmic dynein. It is believed that this motor protein is anchored to the cell membrane and moves nuclei by capturing and pulling on spindle pole body microtubules. To date, four genes hav...

journal_title：Molecular biology of the cell

authors： Chiu YH,Xiang X,Dawe AL,Morris NR

更新日期：1997-09-01 00:00:00

abstract：:Insulin and hypertonicity each increase the content of GLUT4 glucose transporters at the surface of muscle cells. Insulin enhances GLUT4 exocytosis without diminishing its endocytosis. The insulin but not the hypertonicity response is reduced by tetanus neurotoxin, which cleaves vesicle-associated membrane protein (VA...

journal_title：Molecular biology of the cell

authors： Randhawa VK,Thong FS,Lim DY,Li D,Garg RR,Rudge R,Galli T,Rudich A,Klip A

更新日期：2004-12-01 00:00:00

abstract：:The kinesin superfamily of microtubule motor proteins is important in many cellular processes, including mitosis and meiosis, vesicle transport, and the establishment and maintenance of cell polarity. We have characterized two related kinesins in fission yeast, klp5+ and klp6+,, that are amino-terminal motors of the K...

journal_title：Molecular biology of the cell

authors： West RR,Malmstrom T,Troxell CL,McIntosh JR

更新日期：2001-12-01 00:00:00

abstract：:Junctional integrity of endothelial monolayers is crucial to control movement of molecules and cells across the endothelium. Examining the structure and dynamics of cell junctions in endothelial monolayers, we discovered a role for septins. Contacts between adjacent endothelial cells were dynamic, with protrusions ext...

journal_title：Molecular biology of the cell

authors： Kim J,Cooper JA

更新日期：2018-07-15 00:00:00

abstract：:Endoplasmic reticulum-localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum-associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from the ER lumen under conditions of ER stress. To elucidate the function of ERd...

journal_title：Molecular biology of the cell

authors： Fritz JM,Dong M,Apsley KS,Martin EP,Na CL,Sitaraman S,Weaver TE

更新日期：2014-02-01 00:00:00

abstract：:The role of plasma membrane (PM) area as a critical factor during cell motility is poorly understood, mainly due to an inability to precisely follow PM area dynamics. To address this fundamental question, we developed static and dynamic assays to follow exocytosis, endocytosis, and PM area changes during fibroblast sp...

journal_title：Molecular biology of the cell

authors： Gauthier NC,Rossier OM,Mathur A,Hone JC,Sheetz MP

更新日期：2009-07-01 00:00:00

abstract：:Several membrane-associating signals, including covalently linked fatty acids, are found in various combinations at the N termini of signaling proteins. The function of these combinations was investigated by appending fatty acylated N-terminal sequences to green fluorescent protein (GFP). Myristoylated plus mono/dipal...

journal_title：Molecular biology of the cell

authors： McCabe JB,Berthiaume LG

更新日期：1999-11-01 00:00:00

abstract：:The formin mDia2 mediates the formation of lamellipodia and filopodia during cell locomotion. The subcellular localization of activated mDia2 depends on interactions with actin filaments and the plasma membrane. We investigated the poorly understood mechanism of plasma membrane targeting of mDia2 and found that the en...

journal_title：Molecular biology of the cell

authors： Gorelik R,Yang C,Kameswaran V,Dominguez R,Svitkina T

更新日期：2011-01-15 00:00:00

abstract：:Although p38 activity is reported to be required as cells enter mitosis for proper spindle assembly and checkpoint function, its role during the division process remains controversial in lieu of direct data. We therefore conducted live cell studies to determine the effect on mitosis of inhibiting or depleting p38. We ...

journal_title：Molecular biology of the cell

authors： Lee K,Kenny AE,Rieder CL

更新日期：2010-07-01 00:00:00

abstract：:Protein quality control is an essential function of the endoplasmic reticulum. Misfolded proteins unable to acquire their native conformation are retained in the endoplasmic reticulum, retro-translocated back into the cytosol, and degraded via the ubiquitin-proteasome system. We show that efficient degradation of solu...

journal_title：Molecular biology of the cell

authors： Taxis C,Vogel F,Wolf DH

更新日期：2002-06-01 00:00:00

abstract：:Autophagy is a conserved eukaryotic pathway critical for cellular adaptation to changes in nutrition levels and stress. The class III phosphatidylinositol (PI)3-kinase complexes I and II (PI3KC3-C1 and -C2) are essential for autophagosome initiation and maturation, respectively, from highly curved vesicles. We used a ...

journal_title：Molecular biology of the cell

authors： Brier LW,Ge L,Stjepanovic G,Thelen AM,Hurley JH,Schekman R

更新日期：2019-04-15 00:00:00

abstract：:Three mitochondrial DNA-encoded proteins, Cox1, Cox2, and Cox3, comprise the core of the cytochrome c oxidase complex. Gene-specific translational activators ensure that these respiratory chain subunits are synthesized at the correct location and in stoichiometric ratios to prevent unassembled protein products from ge...

journal_title：Molecular biology of the cell

authors： Roloff GA,Henry MF

更新日期：2015-08-15 00:00:00

abstract：:Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises from light excitation. An alternative is luciferase, an enzyme that ...

journal_title：Molecular biology of the cell

authors： Mazo-Vargas A,Park H,Aydin M,Buchler NE

更新日期：2014-11-05 00:00:00

abstract：:Differentiation and polarization of epithelial cells depends on the formation of the apical junctional complex (AJC), which is composed of the tight junction (TJ) and the adherens junction (AJ). In this study, we investigated mechanisms of actin reorganization that drive the establishment of AJC. Using a calcium switc...

journal_title：Molecular biology of the cell

authors： Ivanov AI,Hunt D,Utech M,Nusrat A,Parkos CA

更新日期：2005-06-01 00:00:00

abstract：:The budding yeast formins Bni1 and Bnr1 control the assembly of actin cables. These formins exhibit distinct patterns of localization and polymerize two different populations of cables: Bni1 in the bud and Bnr1 in the mother cell. We generated a functional Bni1-3GFP that improved the visualization of Bni1 in vivo at e...

journal_title：Molecular biology of the cell

authors： Buttery SM,Yoshida S,Pellman D

更新日期：2007-05-01 00:00:00

abstract：:In Schizosaccharomyces pombe, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring (CR). A single essential formin, Cdc12, localizes to the cell middle upon mitotic onset and nucleates the F-actin of the CR. Cdc12 medial recruitment is mediated in part by its direct binding to the...

journal_title：Molecular biology of the cell

authors： Willet AH,Bohnert KA,Gould KL

更新日期：2018-03-15 00:00:00

abstract：:Stimulation of the T-cell receptor (TCR) results in the activation of several transcription factors, including NF-kappaB, that are crucial for T-cell proliferation and gain of effector functions. On TCR engagement, several proteins within the TCR-directed NF-kappaB signaling pathway undergo dynamic spatial redistribut...

journal_title：Molecular biology of the cell

authors： Rossman JS,Stoicheva NG,Langel FD,Patterson GH,Lippincott-Schwartz J,Schaefer BC

更新日期：2006-05-01 00:00:00

abstract：:We have addressed the question of whether or not Golgi fragmentation, as exemplified by that occurring during drug-induced microtubule depolymerization, is accompanied by the separation of Golgi subcompartments one from another. Scattering kinetics of Golgi subcompartments during microtubule disassembly and reassembly...

journal_title：Molecular biology of the cell

authors： Yang W,Storrie B

更新日期：1998-01-01 00:00:00

abstract：:The TOM (translocase of the outer mitochondrial membrane) complex of the outer mitochondrial membrane is required for the import of proteins into the organelle. The core TOM complex contains five proteins, including three small components Tom7, Tom6, and Tom5. We have created single and double mutants of all combinati...

journal_title：Molecular biology of the cell

authors： Sherman EL,Go NE,Nargang FE

更新日期：2005-09-01 00:00:00

abstract：:The Schizosaccharomyces pombe sts1+ gene, identified by supersensitive mutations to a protein kinase inhibitor, staurosporine, was isolated by complementation by the use of a fission yeast genomic library. Nucleotide sequencing shows that the sts1+ gene encodes a 453 amino acid putative membrane-associated protein tha...

journal_title：Molecular biology of the cell

authors： Shimanuki M,Goebl M,Yanagida M,Toda T

更新日期：1992-03-01 00:00:00

abstract：:The gene encoding NFKB1 is autoregulated, responding to NF-kappa B/Rel activation through NF-kappa B binding sites in its promoter, which also contains putative sites for Ets proteins. One of the Ets sites, which we refer to as EBS4, is located next to an NF-kappa B/Rel binding site, kB3, which is absolutely required ...

journal_title：Molecular biology of the cell

authors： Lambert PF,Ludford-Menting MJ,Deacon NJ,Kola I,Doherty RR

更新日期：1997-02-01 00:00:00

abstract：:In cycling between the mammalian host and the tsetse fly vector, trypanosomes undergo major changes in energy metabolism and surface coat composition. Early procyclic (insect) forms in the tsetse fly midgut are coated by glycoproteins known as EP and GPEET procyclins. EP expression continues in late procyclic forms, w...

journal_title：Molecular biology of the cell

authors： Vassella E,Probst M,Schneider A,Studer E,Renggli CK,Roditi I

更新日期：2004-09-01 00:00:00

abstract：:The mechanisms and receptors involved in phagocytosis by nonhematopoietic cells are not well understood. The involvement of the alpha 3 beta 1 integrin in phagocytosis of the extracellular matrix by human breast cancer cells was studied. The possible role of this integrin was suggested since alpha 3 and beta 1 but not...

journal_title：Molecular biology of the cell

authors： Coopman PJ,Thomas DM,Gehlsen KR,Mueller SC

更新日期：1996-11-01 00:00:00

abstract：:Oncoprotein 18/stathmin (Op18) has been identified recently as a protein that destabilizes microtubules, but the mechanism of destabilization is currently controversial. Based on in vitro microtubule assembly assays, evidence has been presented supporting conflicting destabilization models of either tubulin sequestrat...

journal_title：Molecular biology of the cell

authors： Howell B,Larsson N,Gullberg M,Cassimeris L

更新日期：1999-01-01 00:00:00

abstract：:Ghrelin is an acylated peptidyl gastric hormone acting on the pituitary and hypothalamus to stimulate appetite, adiposity, and growth hormone release, through activation of growth hormone secretagogue receptor (GHSR)-1a receptor. Moreover, ghrelin features several activities such as inhibition of apoptosis, regulation...

journal_title：Molecular biology of the cell

authors： Filigheddu N,Gnocchi VF,Coscia M,Cappelli M,Porporato PE,Taulli R,Traini S,Baldanzi G,Chianale F,Cutrupi S,Arnoletti E,Ghè C,Fubini A,Surico N,Sinigaglia F,Ponzetto C,Muccioli G,Crepaldi T,Graziani A

更新日期：2007-03-01 00:00:00

abstract：:In this study, we investigated the role of phospholipase D (PLD) in mediating Arf6 function in cells. Expression of Arf6 mutants that are defective in activating PLD, Arf6N48R and Arf6N48I, inhibited membrane recycling to the plasma membrane (PM), resulting in an accumulation of tubular endosomal membranes. Additional...

journal_title：Molecular biology of the cell

authors： Jovanovic OA,Brown FD,Donaldson JG

更新日期：2006-01-01 00:00:00

abstract：:MAL, a compact hydrophobic, four-transmembrane-domain apical protein that copurifies with detergent-resistant membranes is obligatory for the machinery that sorts glycophosphatidylinositol (GPI)-anchored proteins and others to the apical membrane in epithelia. The mechanism of MAL function in lipid-raft-mediated apica...

journal_title：Molecular biology of the cell

authors： Magal LG,Yaffe Y,Shepshelovich J,Aranda JF,de Marco Mdel C,Gaus K,Alonso MA,Hirschberg K

更新日期：2009-08-01 00:00:00

abstract：:Sec13p has been thought to be an essential component of the COPII coat, required for exit of proteins from the yeast endoplasmic reticulum (ER). We show herein that normal function of Sec13p was not required for ER exit of the Hsp150 glycoprotein. Hsp150 was secreted to the medium under restrictive conditions in a sec...

journal_title：Molecular biology of the cell

authors： Fatal N,Suntio T,Makarow M

更新日期：2002-12-01 00:00:00

abstract：:We recently determined the nucleotide sequence of the gene encoding p28, a light chain of inner dynein arms of Chlamydomonas axonemes. Here, we show that p28 is the protein encoded by the IDA4 locus. p28, and the dynein heavy chains normally associated with it, are completely absent from the flagella and cell bodies o...

journal_title：Molecular biology of the cell

authors： LeDizet M,Piperno G

更新日期：1995-06-01 00:00:00

abstract：:The mannose 6-phosphate (Man-6-P) lysosomal targeting signal on acid hydrolases is synthesized by the sequential action of uridine 5'-diphosphate-N-acetylglucosamine: lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase) and GlcNAc-1-phosphodiester alpha-N-acetylglucosaminidase ("unco...

journal_title：Molecular biology of the cell

authors： Boonen M,Vogel P,Platt KA,Dahms N,Kornfeld S

更新日期：2009-10-01 00:00:00