Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy.

abstract：:Cdc25, the dual-specificity phosphatase that dephosphorylates the Cdc2-cyclin B complex at mitosis, is highly regulated during the cell cycle. In Xenopus egg extracts, Cdc25 is associated with two isoforms of the 14-3-3 protein. Cdc25 is complexed primarily with 14-3-3epsilon and to a lesser extent with 14-3-3zeta. Th...

journal_title：Molecular biology of the cell

authors： Kumagai A,Yakowec PS,Dunphy WG

更新日期：1998-02-01 00:00:00

abstract：:Timely down-regulation of the evolutionarily conserved protein kinase Swe1 plays an important role in cell cycle control, as Swe1 can block nuclear division through inhibitory phosphorylation of the catalytic subunit of cyclin-dependent kinase. In particular, Swe1 degradation is important for budding yeast cell surviv...

journal_title：Molecular biology of the cell

authors： Raspelli E,Cassani C,Lucchini G,Fraschini R

更新日期：2011-07-01 00:00:00

abstract：:Meiotic recombination differs from mitotic recombination in that DSBs are repaired using homologous chromosomes, rather than sister chromatids. This change in partner choice is due in part to a barrier to sister chromatid repair (BSCR) created by the meiosis-specific kinase, Mek1, in a complex with two other meiosis-s...

journal_title：Molecular biology of the cell

authors： Niu H,Wan L,Baumgartner B,Schaefer D,Loidl J,Hollingsworth NM

更新日期：2005-12-01 00:00:00

abstract：:DEAD box proteins are putative RNA helicases that function in all aspects of RNA metabolism, including translation, ribosome biogenesis, and pre-mRNA splicing. Because many processes involving RNA metabolism are spatially organized within the cell, we examined the subcellular distribution of a human DEAD box protein, ...

journal_title：Molecular biology of the cell

authors： Bléoo S,Sun X,Hendzel MJ,Rowe JM,Packer M,Godbout R

更新日期：2001-10-01 00:00:00

abstract：:Both sporadic and familial Alzheimer's disease (AD) patients exhibit increased chromosome aneuploidy, particularly trisomy 21, in neurons and other cells. Significantly, trisomy 21/Down syndrome patients develop early onset AD pathology. We investigated the mechanism underlying mosaic chromosome aneuploidy in AD and r...

journal_title：Molecular biology of the cell

authors： Granic A,Padmanabhan J,Norden M,Potter H

更新日期：2010-02-15 00:00:00

abstract：:We identified a direct interaction between the neuronal transmembrane protein calsyntenin-1 and the light chain of Kinesin-1 (KLC1). GST pulldowns demonstrated that two highly conserved segments in the cytoplasmic domain of calsyntenin-1 mediate binding to the tetratricopeptide repeats of KLC1. A complex containing ca...

journal_title：Molecular biology of the cell

authors： Konecna A,Frischknecht R,Kinter J,Ludwig A,Steuble M,Meskenaite V,Indermühle M,Engel M,Cen C,Mateos JM,Streit P,Sonderegger P

更新日期：2006-08-01 00:00:00

abstract：:For several decades, the trans-Golgi network (TGN) was considered the most distal stop and hence the ultimate protein-sorting station for distinct apical and basolateral transport carriers that reach their respective surface domains in the direct trafficking pathway. However, recent reports of apical and basolateral c...

journal_title：Molecular biology of the cell

authors： Treyer A,Pujato M,Pechuan X,Müsch A

更新日期：2016-07-15 00:00:00

abstract：:Regulation of Rho GTPase signaling is critical for cell shape determination and polarity. Here, we investigated the role of LRG1, a novel member of the GTPase-activating proteins (GAPs) of Neurospora crassa. LRG1 is essential for apical tip extension and to restrict excessive branch formation in subapical regions of t...

journal_title：Molecular biology of the cell

authors： Vogt N,Seiler S

更新日期：2008-11-01 00:00:00

abstract：:During a search for genes controlling conidial dormancy in Aspergillus fumigatus, two dehydrin-like genes, DprA and DprB, were identified. The deduced proteins had repeated stretches of 23 amino acids that contained a conserved dehydrin-like protein (DPR) motif. Disrupted DprAΔ mutants were hypersensitive to oxidative...

journal_title：Molecular biology of the cell

authors： Wong Sak Hoi J,Lamarre C,Beau R,Meneau I,Berepiki A,Barre A,Mellado E,Read ND,Latgé JP

更新日期：2011-06-01 00:00:00

abstract：:The distinct levels of Rac activity differentially regulate the pattern of intrinsic cell migration. However, it remains unknown how Rac activity is modulated and how the level of Rac activity controls cell migratory behavior. Here we show that Slit-Robo GAP 1 (srGAP1) is a modulator of Rac activity in locomotive cell...

journal_title：Molecular biology of the cell

authors： Yamazaki D,Itoh T,Miki H,Takenawa T

更新日期：2013-11-01 00:00:00

abstract：:The BNIP-2 and Cdc42GAP homology (BCH) domain is a novel regulator for Rho GTPases, but its impact on p50-Rho GTPase-activating protein (p50RhoGAP or Cdc42GAP) in cells remains elusive. Here we show that deletion of the BCH domain from p50RhoGAP enhanced its GAP activity and caused drastic cell rounding. Introducing c...

journal_title：Molecular biology of the cell

authors： Zhou YT,Chew LL,Lin SC,Low BC

更新日期：2010-09-15 00:00:00

abstract：:Regulator of G protein signaling (RGS) proteins inhibit G protein signaling by activating Galpha GTPase activity, but the mechanisms that regulate RGS activity are not well understood. The mammalian R7 binding protein (R7BP) can interact with all members of the R7 family of RGS proteins, and palmitoylation of R7BP can...

journal_title：Molecular biology of the cell

authors： Porter MY,Koelle MR

更新日期：2010-01-15 00:00:00

abstract：:Microtubules are long, slender polymers of αβ-tubulin found in all eukaryotic cells. Tubulins associate longitudinally to form protofilaments, and adjacent protofilaments associate laterally to form the microtubule. In the textbook view, microtubules are 1) composed of 13 protofilaments, 2) arranged in a radial array ...

journal_title：Molecular biology of the cell

authors： Chaaban S,Brouhard GJ

更新日期：2017-11-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef directs virus escape from immune surveillance by subverting host cell intracellular signaling and membrane traffic to down-regulate cell-surface major histocompatibility complex class I (MHC-I). The interaction of Nef with the sorting proteins PACS-...

journal_title：Molecular biology of the cell

authors： Dikeakos JD,Thomas L,Kwon G,Elferich J,Shinde U,Thomas G

更新日期：2012-06-01 00:00:00

abstract：:Rab2 immunolocalizes to pre-Golgi intermediates (vesicular-tubular clusters [VTCs]) that are the first site of segregation of anterograde- and retrograde-transported proteins and a major peripheral site for COPI recruitment. Our previous work showed that Rab2 Q65L (equivalent to Ras Q61L) inhibited endoplasmic reticul...

journal_title：Molecular biology of the cell

authors： Tisdale EJ

更新日期：1999-06-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) deletions are associated with various mitochondrial disorders. The deletions identified in humans are flanked by short, directly repeated mitochondrial DNA sequences; however, the mechanism of such DNA rearrangements has yet to be elucidated. In contrast to nuclear DNA (nDNA), mtDNA is more e...

journal_title：Molecular biology of the cell

authors： Tadi SK,Sebastian R,Dahal S,Babu RK,Choudhary B,Raghavan SC

更新日期：2016-01-15 00:00:00

abstract：:Hedgehog (Hh) is a secreted morphogen involved in both short- and long-range signaling necessary for tissue patterning during development. It is unclear how this dually lipidated protein is transported over a long range in the aqueous milieu of interstitial spaces. We previously showed that the long-range signaling of...

journal_title：Molecular biology of the cell

authors： Parchure A,Vyas N,Ferguson C,Parton RG,Mayor S

更新日期：2015-12-15 00:00:00

abstract：:Oncostatin M and cAMP signaling stimulate apical surface-directed membrane trafficking and apical lumen development in hepatocytes, both in a protein kinase A (PKA)-dependent manner. Here, we show that oncostatin M, but not cAMP, promotes the A-kinase anchoring protein (AKAP)-dependent anchoring of the PKA regulatory ...

journal_title：Molecular biology of the cell

authors： Wojtal KA,Hoekstra D,van Ijzendoorn SC

更新日期：2007-07-01 00:00:00

abstract：:Myc interacting zinc finger protein-1 (Miz1) is a transcription factor known to regulate cell cycle- and cell adhesion-related genes in cancer. Here we show that Miz1 also plays a critical role in neural crest development. In the chick, Miz1 is expressed throughout the neural plate and closing neural tube. Its morphol...

journal_title：Molecular biology of the cell

authors： Kerosuo L,Bronner ME

更新日期：2014-02-01 00:00:00

abstract：:Apoptosis causes characteristic morphological changes in cells, including membrane blebbing, cell detachment from the extracellular matrix, and loss of cell-cell contacts. We investigated the changes in focal adhesion proteins during etoposide-induced apoptosis in Rat-1 cells and found that during apoptosis, p130cas (...

journal_title：Molecular biology of the cell

authors： Kook S,Shim SR,Choi SJ,Ahnn J,Kim JI,Eom SH,Jung YK,Paik SG,Song WK

更新日期：2000-03-01 00:00:00

abstract：:In pancreatic β-cells, glutamate dehydrogenase (GDH) modulates insulin secretion, although its function regarding specific secretagogues is unclear. This study investigated the role of GDH using a β-cell-specific GDH knockout mouse model, called βGlud1(-/-). The absence of GDH in islets isolated from βGlud1(-/-) mice ...

journal_title：Molecular biology of the cell

authors： Vetterli L,Carobbio S,Pournourmohammadi S,Martin-Del-Rio R,Skytt DM,Waagepetersen HS,Tamarit-Rodriguez J,Maechler P

更新日期：2012-10-01 00:00:00

abstract：:Of the three structural maintenance of chromosomes (SMC) complexes, Smc5/6 remains the most poorly understood. Genetic studies have shown that Smc5/6 mutants are defective in homologous recombination (HR), and consistent with this, Smc5/6 is enriched at lesions. However, Smc5/6 is essential for viability, but HR is no...

journal_title：Molecular biology of the cell

authors： Tapia-Alveal C,O'Connell MJ

更新日期：2011-12-01 00:00:00

abstract：:Cysteine string protein (Csp) is essential for neurotransmitter release in Drosophila. It has been suggested that Csp functions by regulating the activity of presynaptic Ca2+ channels, thus controlling exocytosis. We have examined the effect of overexpressing Csp1 in PC12 cells, a neuroendocrine cell line. PC12 cell c...

journal_title：Molecular biology of the cell

authors： Chamberlain LH,Burgoyne RD

更新日期：1998-08-01 00:00:00

abstract：:Transport of cellular and neuronal vesicles, organelles, and other particles along microtubules requires the molecular motor protein dynein (Mallik and Gross, 2004). Critical to dynein function is dynactin, a multiprotein complex commonly thought to be required for dynein attachment to membrane compartments (Karki and...

journal_title：Molecular biology of the cell

authors： Haghnia M,Cavalli V,Shah SB,Schimmelpfeng K,Brusch R,Yang G,Herrera C,Pilling A,Goldstein LS

更新日期：2007-06-01 00:00:00

abstract：:Tubulogenesis by epithelial cells regulates kidney, lung, and mammary development, whereas that by endothelial cells regulates vascular development. Although functionally dissimilar, the processes necessary for tubulation by epithelial and endothelial cells are very similar. We performed microarray analysis to further...

journal_title：Molecular biology of the cell

authors： Albig AR,Schiemann WP

更新日期：2005-02-01 00:00:00

abstract：:Disassembly of the epithelial apical junctional complex (AJC), composed of the tight junction (TJ) and adherens junction (AJ), is important for normal tissue remodeling and pathogen-induced disruption of epithelial barriers. Using a calcium depletion model in T84 epithelial cells, we previously found that disassembly ...

journal_title：Molecular biology of the cell

authors： Ivanov AI,McCall IC,Parkos CA,Nusrat A

更新日期：2004-06-01 00:00:00

abstract：:Mutations in the cytolinker protein plectin lead to grossly distorted morphology of neuromuscular junctions (NMJs) in patients suffering from epidermolysis bullosa simplex (EBS)-muscular dystrophy (MS) with myasthenic syndrome (MyS). Here we investigated whether plectin contributes to the structural integrity of NMJs ...

journal_title：Molecular biology of the cell

authors： Mihailovska E,Raith M,Valencia RG,Fischer I,Al Banchaabouchi M,Herbst R,Wiche G

更新日期：2014-12-15 00:00:00

abstract：:In animal cells, positioning of the mitotic spindle is crucial for defining the plane of cytokinesis and the size ratio of daughter cells. We have characterized this phenomenon in a rat epithelial cell line using microscopy, micromanipulation, and microinjection. Unmanipulated cells position the mitotic spindle near t...

journal_title：Molecular biology of the cell

authors： O'Connell CB,Wang YL

更新日期：2000-05-01 00:00:00

abstract：:Differentiation-inducing factor-1 (DIF-1) is a polyketide that induces Dictyostelium amoebae to differentiate as prestalk cells. We performed a global quantitative screen for phosphorylation changes that occur within the first minutes after addition of DIF-1, using a triple-label SILAC approach. This revealed a new wo...

journal_title：Molecular biology of the cell

authors： Sugden C,Urbaniak MD,Araki T,Williams JG

更新日期：2015-02-15 00:00:00

abstract：:Vac8p is a vacuolar membrane protein that is required for efficient vacuole inheritance and fusion, cytosol-to-vacuole targeting, and sporulation. By analogy to other armadillo domain proteins, including beta-catenin and importin alpha, we hypothesize that Vac8p docks various factors at the vacuole membrane. Two-hybri...

journal_title：Molecular biology of the cell

authors： Pan X,Roberts P,Chen Y,Kvam E,Shulga N,Huang K,Lemmon S,Goldfarb DS

更新日期：2000-07-01 00:00:00