The BNIP-2 and Cdc42GAP homology (BCH) domain of p50RhoGAP/Cdc42GAP sequesters RhoA from inactivation by the adjacent GTPase-activating protein domain.

abstract：:Cadherin-mediated cell-cell adhesion is a dynamic process that is regulated during embryonic development, cell migration, and differentiation. Different cadherins are expressed in specific tissues consistent with their roles in cell type recognition. In this study, we examine the formation of N-cadherin-dependent cell...

journal_title：Molecular biology of the cell

authors： Mary S,Charrasse S,Meriane M,Comunale F,Travo P,Blangy A,Gauthier-Rouvière C

更新日期：2002-01-01 00:00:00

abstract：:Multisite phosphorylation of proteins is a powerful signal processing mechanism that plays crucial roles in cell division and differentiation as well as in disease. We recently demonstrated a novel phenomenon in cell cycle regulation by showing that cyclin-dependent kinase-dependent multisite phosphorylation of a cruc...

journal_title：Molecular biology of the cell

authors： Valk E,Venta R,Ord M,Faustova I,Kõivomägi M,Loog M

更新日期：2014-11-05 00:00:00

abstract：:In animal cells, positioning of the mitotic spindle is crucial for defining the plane of cytokinesis and the size ratio of daughter cells. We have characterized this phenomenon in a rat epithelial cell line using microscopy, micromanipulation, and microinjection. Unmanipulated cells position the mitotic spindle near t...

journal_title：Molecular biology of the cell

authors： O'Connell CB,Wang YL

更新日期：2000-05-01 00:00:00

abstract：:Trimming of N-linked oligosaccharides by endoplasmic reticulum (ER) glucosidase II is implicated in quality control of protein folding. An alternate glucosidase II-independent deglucosylation pathway exists, in which endo-alpha-mannosidase cleaves internally the glucose-substituted mannose residue of oligosaccharides....

journal_title：Molecular biology of the cell

authors： Zuber C,Spiro MJ,Guhl B,Spiro RG,Roth J

更新日期：2000-12-01 00:00:00

abstract：:Saccharomyces cerevisiae mating pheromones induce production of Afr1p, a protein that negatively regulates pheromone receptor signaling and is required for normal formation of the projection of cell growth that becomes the site of cell fusion during conjugation. Afr1p interacts with Cdc12p, which belongs to a family o...

journal_title：Molecular biology of the cell

authors： Giot L,Konopka JB

更新日期：1997-06-01 00:00:00

abstract：:Both sporadic and familial Alzheimer's disease (AD) patients exhibit increased chromosome aneuploidy, particularly trisomy 21, in neurons and other cells. Significantly, trisomy 21/Down syndrome patients develop early onset AD pathology. We investigated the mechanism underlying mosaic chromosome aneuploidy in AD and r...

journal_title：Molecular biology of the cell

authors： Granic A,Padmanabhan J,Norden M,Potter H

更新日期：2010-02-15 00:00:00

abstract：:The mitochondrial cytochrome c oxidase assembles in the inner membrane from subunits of dual genetic origin. The assembly process of the enzyme is initiated by membrane insertion of the mitochondria-encoded Cox1 subunit. During complex maturation, transient assembly intermediates, consisting of structural subunits and...

journal_title：Molecular biology of the cell

authors： Bareth B,Nikolov M,Lorenzi I,Hildenbeutel M,Mick DU,Helbig C,Urlaub H,Ott M,Rehling P,Dennerlein S

更新日期：2016-05-15 00:00:00

abstract：:The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disru...

journal_title：Molecular biology of the cell

authors： Ahat E,Xiang Y,Zhang X,Bekier ME 2nd,Wang Y

更新日期：2019-03-15 00:00:00

abstract：:Differentiation-inducing factor-1 (DIF-1) is a polyketide that induces Dictyostelium amoebae to differentiate as prestalk cells. We performed a global quantitative screen for phosphorylation changes that occur within the first minutes after addition of DIF-1, using a triple-label SILAC approach. This revealed a new wo...

journal_title：Molecular biology of the cell

authors： Sugden C,Urbaniak MD,Araki T,Williams JG

更新日期：2015-02-15 00:00:00

abstract：:Cytokinesis requires the coordination of many cellular complexes, particularly those involved in the constriction and reconstruction of the plasma membrane in the cleavage furrow. We have investigated the regulation and function of vesicle transport and fusion during cytokinesis in budding yeast. By using time-lapse c...

journal_title：Molecular biology of the cell

authors： VerPlank L,Li R

更新日期：2005-05-01 00:00:00

abstract：:Shiga-toxin-producing Escherichia coli remain a food-borne health threat. Shiga toxin is endocytosed by intestinal epithelial cells and transported retrogradely through the secretory pathway. It is ultimately translocated to the cytosol where it inhibits protein translation. We found that Shiga toxin transport through...

journal_title：Molecular biology of the cell

authors： Hehnly H,Longhini KM,Chen JL,Stamnes M

更新日期：2009-10-01 00:00:00

abstract：:Drosophila myosin IB (Myo1B) is one of two class I myosins in the Drosophila genome. In the larval and adult midgut enterocyte, Myo1B is present within the microvillus (MV) of the apical brush border (BB) where it forms lateral tethers between the MV membrane and underlying actin filament core. Expression of green flu...

journal_title：Molecular biology of the cell

authors： Hegan PS,Mermall V,Tilney LG,Mooseker MS

更新日期：2007-11-01 00:00:00

abstract：:Dog thyroid epithelial cells in primary culture constitute a physiologically relevant model of positive control of DNA synthesis initiation and G0-S prereplicative phase progression by cAMP as a second messenger for thyrotropin (thyroid-stimulating hormone [TSH]). As previously shown in this system, the cAMP-dependent...

journal_title：Molecular biology of the cell

authors： Depoortere F,Pirson I,Bartek J,Dumont JE,Roger PP

更新日期：2000-03-01 00:00:00

abstract：:We used transgenic analysis in Drosophila to compare the ability of two structurally similar alpha-tubulin isoforms to support microtubule assembly in vivo. Our data revealed that even closely related alpha-tubulin isoforms have different functional capacities. Thus, in multicellular organisms, even small changes in t...

journal_title：Molecular biology of the cell

authors： Hutchens JA,Hoyle HD,Turner FR,Raff EC

更新日期：1997-03-01 00:00:00

abstract：:A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of th...

journal_title：Molecular biology of the cell

authors： Midwood KS,Schwarzbauer JE

更新日期：2002-10-01 00:00:00

abstract：:The architecture of the cytoskeleton and its remodeling are tightly regulated by dynamic reorganization of keratin-rich intermediate filaments. Plakin family proteins associate with the network of intermediate filaments (IFs) and affect its reorganization during migration, differentiation, and response to stress. The ...

journal_title：Molecular biology of the cell

authors： Gujrati M,Mittal R,Ekal L,Mishra RK

更新日期：2019-02-01 00:00:00

abstract：:The proper positioning of mitotic spindle in the single-cell Caenorhabditis elegans embryo is achieved initially by the migration and rotation of the pronuclear complex (PNC) and its two associated astral microtubules (MTs). Pronuclear migration produces global cytoplasmic flows that couple the mechanics of all MTs, t...

journal_title：Molecular biology of the cell

authors： Nazockdast E,Rahimian A,Needleman D,Shelley M

更新日期：2017-11-07 00:00:00

abstract：:The type III transforming growth factor β (TGF-β) receptor (TβRIII), also known as betaglycan, is the most abundantly expressed TGF-β receptor. TβRIII suppresses breast cancer progression by inhibiting migration, invasion, metastasis, and angiogenesis. TβRIII binds TGF-β ligands, with membrane-bound TβRIII presenting ...

journal_title：Molecular biology of the cell

authors： Elderbroom JL,Huang JJ,Gatza CE,Chen J,How T,Starr M,Nixon AB,Blobe GC

更新日期：2014-08-15 00:00:00

abstract：:Previous research has found that a gamma-tubulin mutation in Schizosaccharomyces pombe is synthetically lethal with a deletion of the C-terminal motor domain kinesin-like protein gene pkl1, but the lethality of the double mutant prevents a phenotypic analysis of the synthetic interaction. We have investigated interact...

journal_title：Molecular biology of the cell

authors： Prigozhina NL,Walker RA,Oakley CE,Oakley BR

更新日期：2001-10-01 00:00:00

abstract：:Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the clathrin adaptor complex AP-1 is involved in retroviral release. The absence of AP-1mu obtained by genetic knock-out or b...

journal_title：Molecular biology of the cell

authors： Camus G,Segura-Morales C,Molle D,Lopez-Vergès S,Begon-Pescia C,Cazevieille C,Schu P,Bertrand E,Berlioz-Torrent C,Basyuk E

更新日期：2007-08-01 00:00:00

abstract：:In this essay I describe my personal journey from reductionist to systems cell biology and describe how this in turn led to a 3-year sea voyage to explore complex ocean communities. In describing this journey, I hope to convey some important principles that I gleaned along the way. I realized that cellular functions e...

journal_title：Molecular biology of the cell

authors： Karsenti E

更新日期：2012-07-01 00:00:00

abstract：:Fibroblast growth factor (FGF) receptors (FGFRs) signal to modulate diverse cellular functions, including epithelial cell morphogenesis. In epithelial cells, E-cadherin plays a key role in cell-cell adhesion, and its function can be regulated through endocytic trafficking. In this study, we investigated the location, ...

journal_title：Molecular biology of the cell

authors： Bryant DM,Wylie FG,Stow JL

更新日期：2005-01-01 00:00:00

abstract：:Ubiquitination is a reversible modification that influences a broad range of physiological processes. There are approximately 90 deubiquitinases (DUBs) encoded in the human genome, of which 79 are predicted to have catalytic activity. We tagged 66 DUBs with green fluorescent protein and systematically surveyed their s...

journal_title：Molecular biology of the cell

authors： Urbé S,Liu H,Hayes SD,Heride C,Rigden DJ,Clague MJ

更新日期：2012-03-01 00:00:00

abstract：:Chediak-Higashi syndrome is a genetic disorder caused by mutations in a gene encoding a protein named LYST in humans ("lysosomal trafficking regulator") or Beige in mice. A prominent feature of this disease is the accumulation of enlarged lysosome-related granules in a variety of cells. The genome of Dictyostelium dis...

journal_title：Molecular biology of the cell

authors： Harris E,Wang N,Wu Wl WL,Weatherford A,De Lozanne A,Cardelli J

更新日期：2002-02-01 00:00:00

abstract：:In kinetochores, the Ndc80 complex couples the energy in a depolymerizing microtubule to perform the work of moving chromosomes. The complex directly binds microtubules using an unstructured, positively charged N-terminal tail located on Hec1/Ndc80. Hec1/Ndc80 also contains a calponin homology domain (CHD) that increa...

journal_title：Molecular biology of the cell

authors： Tooley JG,Miller SA,Stukenberg PT

更新日期：2011-04-15 00:00:00

abstract：:The mechanisms and receptors involved in phagocytosis by nonhematopoietic cells are not well understood. The involvement of the alpha 3 beta 1 integrin in phagocytosis of the extracellular matrix by human breast cancer cells was studied. The possible role of this integrin was suggested since alpha 3 and beta 1 but not...

journal_title：Molecular biology of the cell

authors： Coopman PJ,Thomas DM,Gehlsen KR,Mueller SC

更新日期：1996-11-01 00:00:00

abstract：:Polarized cell growth requires the coupling of a defined spatial site on the cell cortex to the apparatus that directs the establishment of cell polarity. In the budding yeast Saccharomyces cerevisiae, the Ras-family GTPase Rsr1p/Bud1p and its regulators select the proper site for bud emergence on the cell cortex. The...

journal_title：Molecular biology of the cell

authors： Kozminski KG,Beven L,Angerman E,Tong AH,Boone C,Park HO

更新日期：2003-12-01 00:00:00

abstract：:The chromosomal passenger complex (CPC) regulates various events in cell division. This complex is composed of a catalytic subunit, Aurora B kinase, and three nonenzymatic subunits, INCENP, Survivin, and Borealin. Together, these four subunits interdependently regulate CPC function, and they are highly conserved among...

journal_title：Molecular biology of the cell

authors： Bohnert KA,Chen JS,Clifford DM,Vander Kooi CW,Gould KL

更新日期：2009-08-01 00:00:00

abstract：:The PDZ domain-containing protein CAL mediates lysosomal trafficking and degradation of CFTR. Here we demonstrate the involvement of a CAL-binding SNARE protein syntaxin 6 (STX6) in this process. Overexpression of STX6, which colocalizes and coimmunoprecipitates with CAL, dramatically reduces the steady-state level an...

journal_title：Molecular biology of the cell

authors： Cheng J,Cebotaru V,Cebotaru L,Guggino WB

更新日期：2010-04-01 00:00:00

abstract：:Cancer cell migration requires the regulation of actin networks at protrusions associated with invadopodia and other leading edges. Carcinomas become invasive after undergoing an epithelial-mesenchymal transition characterized by the appearance of vimentin filaments. While vimentin expression correlates with cell migr...

journal_title：Molecular biology of the cell

authors： Lanier MH,Kim T,Cooper JA

更新日期：2015-12-15 00:00:00