Retrograde Shiga toxin trafficking is regulated by ARHGAP21 and Cdc42.

abstract：:The orderly progression through the cell division cycle is of paramount importance to all organisms, as improper progression through the cycle could result in defects with grave consequences. Previously, our lab has shown that model eukaryotes such as Saccharomyces cerevisiae, Caenorhabditis elegans, and Danio rerio a...

journal_title：Molecular biology of the cell

authors： Chan K,Goldmark JP,Roth MB

更新日期：2010-07-01 00:00:00

abstract：:Glycerol and other polyols are used as osmoprotectants by many organisms. Several yeasts and other fungi can take up glycerol by proton symport. To identify genes involved in active glycerol uptake in Saccharomyces cerevisiae we screened a deletion mutant collection comprising 321 genes encoding proteins with 6 or mor...

journal_title：Molecular biology of the cell

authors： Ferreira C,van Voorst F,Martins A,Neves L,Oliveira R,Kielland-Brandt MC,Lucas C,Brandt A

更新日期：2005-04-01 00:00:00

abstract：:Cell morphogenesis depends on polarized exocytosis. One widely held model posits that long-range transport and exocyst-dependent tethering of exocytic vesicles at the plasma membrane sequentially drive this process. Here, we describe that disruption of either actin-based long-range transport and microtubules or the ex...

journal_title：Molecular biology of the cell

authors： Bendezú FO,Martin SG

更新日期：2011-01-01 00:00:00

abstract：:CLA4, encoding a protein kinase of the PAK type, and CDC11, encoding a septin, were isolated in a screen for synthetic lethality with CHS3, which encodes the chitin synthase III catalytic moiety. Although Ste20p shares some essential function with Cla4p, it did not show synthetic lethality with Chs3p. cla4 and cdc11 m...

journal_title：Molecular biology of the cell

authors： Schmidt M,Varma A,Drgon T,Bowers B,Cabib E

更新日期：2003-05-01 00:00:00

abstract：:The subcellular distribution of kinases and other signaling proteins is regulated in response to cellular cues; however, the extent of this regulation has not been investigated for any gene set in any organism. Here, we present a systematic analysis of protein kinases in the budding yeast, screening for differential l...

journal_title：Molecular biology of the cell

authors： Bharucha N,Ma J,Dobry CJ,Lawson SK,Yang Z,Kumar A

更新日期：2008-07-01 00:00:00

abstract：:Whereas lysosome-related organelles (LRO) of specialized cells display both exocytic and endocytic features, lysosomes in nonspecialized cells can also acquire the property to fuse with the plasma membrane upon an acute rise in cytosolic calcium. Here, we characterize this unconventional secretory pathway in fibroblas...

journal_title：Molecular biology of the cell

authors： Laulagnier K,Schieber NL,Maritzen T,Haucke V,Parton RG,Gruenberg J

更新日期：2011-06-15 00:00:00

abstract：:Lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) and their counterreceptors such as intercellular cell adhesion molecules (ICAM-1 and ICAM-2), junctional adhesion molecules (JAM-A, JAM-C), and receptors for advanced glycation end products (RAGE) are crucial for promoting polymorphonucl...

journal_title：Molecular biology of the cell

authors： Li N,Yang H,Wang M,Lü S,Zhang Y,Long M

更新日期：2018-02-15 00:00:00

abstract：:Basal bodies organize cilia that are responsible for both mechanical beating and sensation. Nascent basal body assembly follows a series of well characterized morphological events; however, the proteins and their assembly dynamics for new basal body formation and function are not well understood. High-resolution light...

journal_title：Molecular biology of the cell

authors： Pearson CG,Giddings TH Jr,Winey M

更新日期：2009-02-01 00:00:00

abstract：:The cation-dependent mannose 6-phosphate receptor (CD-MPR) mediates the transport of lysosomal enzymes from the trans-Golgi network to endosomes. Evasion of lysosomal degradation of the CD-MPR requires reversible palmitoylation of a cysteine residue in its cytoplasmic tail. Because palmitoylation is reversible and ess...

journal_title：Molecular biology of the cell

authors： Stöckli J,Rohrer J

更新日期：2004-06-01 00:00:00

abstract：:Clustered protocadherins (Pcdhs) are arranged in gene clusters (α, β, and γ) with variable and constant exons. Variable exons encode cadherin and transmembrane domains and ~90 cytoplasmic residues. The 14 Pcdh-αs and 22 Pcdh-γs are spliced to constant exons, which, for Pcdh-γs, encode ~120 residues of an identical cyt...

journal_title：Molecular biology of the cell

authors： O'Leary R,Reilly JE,Hanson HH,Kang S,Lou N,Phillips GR

更新日期：2011-11-01 00:00:00

abstract：:Select lipid-anchored proteins such as glycosylphosphatidylinositol (GPI)-anchored proteins and nonreceptor tyrosine kinases may preferentially partition into sphingomyelin-rich and cholesterol-rich plasmalemmal microdomains, thereby acquiring resistance to detergent extraction. Two such domains, caveolae and lipid ra...

journal_title：Molecular biology of the cell

authors： Oh P,Schnitzer JE

更新日期：2001-03-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:Discoidin I expression was used as a marker to screen for mutants affected in the growth-differentiation transition (GDT) of Dictyostelium. By REMI mutagenesis we have isolated mutant 2-9, an overexpressor of discoidin I. It displays normal morphogenesis but shows premature entry into the developmental cycle. The disr...

journal_title：Molecular biology of the cell

authors： Zeng C,Anjard C,Riemann K,Konzok A,Nellen W

更新日期：2000-05-01 00:00:00

abstract：:The Arp2/3 complex is the only known nucleator of branched F-actin filaments. Work in cultured cells has established a wide array of functions for this complex in controlling cell migration, shape, and adhesion. However, loss of Arp2/3 complex function in tissues has yielded cell type-specific phenotypes. Here we repo...

journal_title：Molecular biology of the cell

authors： Zhou K,Sumigray KD,Lechler T

更新日期：2015-06-01 00:00:00

abstract：:Rho GTPases share a common inhibitor, Rho guanine nucleotide dissociation inhibitor (RhoGDI), which regulates their expression levels, membrane localization, and activation state. The selective dissociation of individual Rho GTPases from RhoGDI ensures appropriate responses to cellular signals, but the underlying mech...

journal_title：Molecular biology of the cell

authors： Ard R,Mulatz K,Abramovici H,Maillet JC,Fottinger A,Foley T,Byham MR,Iqbal TA,Yoneda A,Couchman JR,Parks RJ,Gee SH

更新日期：2012-10-01 00:00:00

abstract：:Although the distribution of the cation-independent mannose 6-phosphate receptor (CI-MPR) has been well studied, its intracellular itinerary and trafficking kinetics remain uncertain. In this report, we describe the endocytic trafficking and steady-state localization of a chimeric form of the CI-MPR containing the ect...

journal_title：Molecular biology of the cell

authors： Lin SX,Mallet WG,Huang AY,Maxfield FR

更新日期：2004-02-01 00:00:00

abstract：:CD98, an early marker of T-cell activation, is an important regulator of integrin-mediated adhesion events. Previous studies suggest that CD98 is coupled to both cellular activation and transformation and is involved in the pathogenesis of viral infection, inflammatory disease, and cancer. Understanding of the molecul...

journal_title：Molecular biology of the cell

authors： Rintoul RC,Buttery RC,Mackinnon AC,Wong WS,Mosher D,Haslett C,Sethi T

更新日期：2002-08-01 00:00:00

abstract：:Studies on physiological modulation of intercellular communication mediated by protein kinases are often complicated by the fact that cells express multiple gap junction proteins (connexins; Cx). Changes in cell coupling can be masked by simultaneous opposite regulation of the gap junction channel types expressed. We ...

journal_title：Molecular biology of the cell

authors： Kwak BR,Hermans MM,De Jonge HR,Lohmann SM,Jongsma HJ,Chanson M

更新日期：1995-12-01 00:00:00

abstract：:The c-myb proto-oncogene product (c-Myb) regulates proliferation and differentiation of hematopoietic cells. Recently we have shown that c-Myb is degraded in response to Wnt-1 stimulation via a pathway involving TAK1 (TGF-beta-activated kinase), HIPK2 (homeodomain-interacting protein kinase 2), and NLK (Nemo-like kina...

journal_title：Molecular biology of the cell

authors： Kurahashi T,Nomura T,Kanei-Ishii C,Shinkai Y,Ishii S

更新日期：2005-10-01 00:00:00

abstract：:The mitochondrial Hsp70 chaperone Ssq1 plays a dedicated role in the maturation of iron-sulfur (Fe/S) proteins, an essential process of mitochondria. Similar to its bacterial orthologue HscA, Ssq1 binds to the scaffold protein Isu1, thereby facilitating dissociation of the newly synthesized Fe/S cluster on Isu1 and it...

journal_title：Molecular biology of the cell

authors： Uzarska MA,Dutkiewicz R,Freibert SA,Lill R,Mühlenhoff U

更新日期：2013-06-01 00:00:00

abstract：:During lactation, mammary epithelial cells secrete huge amounts of milk from their apical side. The current view is that caseins are secreted by exocytosis, whereas milk fat globules are released by budding, enwrapped by the plasma membrane. Owing to the number and large size of milk fat globules, the membrane surface...

journal_title：Molecular biology of the cell

authors： Honvo-Houéto E,Henry C,Chat S,Layani S,Truchet S

更新日期：2016-10-01 00:00:00

abstract：:Trafficking through the Golgi apparatus requires members of the Arf family of GTPases, whose activation is regulated by guanine nucleotide exchange factors (GEFs). Once activated, Arf-GTP recruits effectors such as coat complexes and lipid-modifying enzymes to specific membrane sites, creating a domain competent for c...

journal_title：Molecular biology of the cell

authors： Niu TK,Pfeifer AC,Lippincott-Schwartz J,Jackson CL

更新日期：2005-03-01 00:00:00

abstract：:In mammalian cells, the inheritance of the Golgi apparatus into the daughter cells during each cycle of cell division is mediated by a disassembly and reassembly process, and this process is precisely controlled by phosphorylation and ubiquitination. VCIP135 (valosin-containing protein p97/p47 complex-interacting prot...

journal_title：Molecular biology of the cell

authors： Zhang X,Wang Y

更新日期：2015-06-15 00:00:00

abstract：:Maturation of organelles in the endolysosomal pathway requires exchange of the early endosomal GTPase Rab5/Vps21 for the late endosomal Rab7/Ypt7. The Rab exchange depends on the guanine nucleotide exchange factor activity of the Mon1-Ccz1 heterodimer for Ypt7. Here we investigate vacuole binding and recycling of Mon1...

journal_title：Molecular biology of the cell

authors： Lawrence G,Brown CC,Flood BA,Karunakaran S,Cabrera M,Nordmann M,Ungermann C,Fratti RA

更新日期：2014-05-01 00:00:00

abstract：:Regulation of Rho GTPase signaling is critical for cell shape determination and polarity. Here, we investigated the role of LRG1, a novel member of the GTPase-activating proteins (GAPs) of Neurospora crassa. LRG1 is essential for apical tip extension and to restrict excessive branch formation in subapical regions of t...

journal_title：Molecular biology of the cell

authors： Vogt N,Seiler S

更新日期：2008-11-01 00:00:00

abstract：:Oncostatin M and cAMP signaling stimulate apical surface-directed membrane trafficking and apical lumen development in hepatocytes, both in a protein kinase A (PKA)-dependent manner. Here, we show that oncostatin M, but not cAMP, promotes the A-kinase anchoring protein (AKAP)-dependent anchoring of the PKA regulatory ...

journal_title：Molecular biology of the cell

authors： Wojtal KA,Hoekstra D,van Ijzendoorn SC

更新日期：2007-07-01 00:00:00

abstract：:Tumor initiation and growth is associated with significant changes in the surrounding tissue. During carcinoma progression, a global stiffening of the extracellular matrix is observed and is interpreted as a signature of aggressive invasive tumors. However, it is still unknown whether this increase in matrix rigidity ...

journal_title：Molecular biology of the cell

authors： Staneva R,Burla F,Koenderink GH,Descroix S,Vignjevic DM,Attieh Y,Verhulsel M

更新日期：2018-12-01 00:00:00

abstract：:Sustained directional migration of epithelial cells is essential for regeneration of injured epithelia. Front-rear polarity of migrating cells is determined by local activation of a signaling network involving Cdc42 and other factors in response to spatial cues from the environment, the nature of which are obscure. We...

journal_title：Molecular biology of the cell

authors： Greciano PG,Moyano JV,Buschmann MM,Tang J,Lu Y,Rudnicki J,Manninen A,Matlin KS

更新日期：2012-01-01 00:00:00

abstract：:Many biological systems respond to environmental changes by activating intracellular signaling cascades, resulting in an appropriate response. One such system is represented by the social amoeba Dictyostelium discoideum. When food sources become scarce, these unicellular cells can initiate a cAMP-driven multicellular ...

journal_title：Molecular biology of the cell

authors： Vinet AF,Fiedler T,Studer V,Froquet R,Dardel A,Cosson P,Pieters J

更新日期：2014-03-01 00:00:00

abstract：:The membrane-anchored atlastin GTPase couples nucleotide hydrolysis to the catalysis of homotypic membrane fusion to form a branched endoplasmic reticulum network. Trans dimerization between atlastins anchored in opposing membranes, accompanied by a cross-over conformational change, is thought to draw the membranes to...

journal_title：Molecular biology of the cell

authors： Saini SG,Liu C,Zhang P,Lee TH

更新日期：2014-12-01 00:00:00