Abstract:
:Sustained directional migration of epithelial cells is essential for regeneration of injured epithelia. Front-rear polarity of migrating cells is determined by local activation of a signaling network involving Cdc42 and other factors in response to spatial cues from the environment, the nature of which are obscure. We examined the roles of laminin (LM)-511 and LM-332, two structurally different laminin isoforms, in the migration of Madin-Darby canine kidney cells by suppressing expression of their α subunits using RNA interference. We determined that knockdown of LM-511 inhibits directional migration and destabilizes cell-cell contacts, in part by disturbing the localization and activity of the polarization machinery. Suppression of integrin α3, a laminin receptor subunit, in cells synthesizing normal amounts of both laminins has a similar effect as knockdown of LM-511. Surprisingly, simultaneous suppression of both laminin α5 and laminin α3 restores directional migration and cell-cell contact stability, suggesting that cells recognize a haptotactic gradient formed by a combination of laminins.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Greciano PG,Moyano JV,Buschmann MM,Tang J,Lu Y,Rudnicki J,Manninen A,Matlin KSdoi
10.1091/mbc.E11-08-0718subject
Has Abstractpub_date
2012-01-01 00:00:00pages
121-36issue
1eissn
1059-1524issn
1939-4586pii
mbc.E11-08-0718journal_volume
23pub_type
杂志文章abstract::Colony-stimulating factor-1 (CSF-1) induces expression of immediate early gene, such as c-myc and c-fos and delayed early genes such as D-type cyclins (D1 and D2), whose products play essential roles in the G1 to S phase transition of the cell cycle. Little is known, however, about the cytoplasmic signal transduction ...
journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.11.11.3835
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journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.e05-11-1019
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journal_title:Molecular biology of the cell
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更新日期:2012-06-01 00:00:00
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journal_title:Molecular biology of the cell
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journal_title:Molecular biology of the cell
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journal_title:Molecular biology of the cell
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doi:10.1091/mbc.E18-09-0579
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journal_title:Molecular biology of the cell
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doi:10.1091/mbc.10.11.3909
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1091/mbc.e10-01-0060
更新日期:2010-05-15 00:00:00
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journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.E10-08-0715
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journal_title:Molecular biology of the cell
pub_type: 杂志文章
doi:10.1091/mbc.E12-06-0471
更新日期:2013-03-01 00:00:00
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journal_title:Molecular biology of the cell
pub_type: 杂志文章
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journal_title:Molecular biology of the cell
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journal_title:Molecular biology of the cell
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pub_type: 杂志文章
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journal_title:Molecular biology of the cell
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular biology of the cell
pub_type: 杂志文章
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pub_type: 杂志文章
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