Abstract:
:The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail to measure the affinity of the reactants for each other. This essay describes simple methods to get the most out of valuable reagents in binding experiments.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Pollard TDdoi
10.1091/mbc.E10-08-0683subject
Has Abstractpub_date
2010-12-01 00:00:00pages
4061-7issue
23eissn
1059-1524issn
1939-4586pii
21/23/4061journal_volume
21pub_type
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