Abstract:
:Early endosomes in PC12 cells are an important site for the formation of synaptic-like microvesicles and constitutive recycling vesicles. By immunogold electron microscopy, the small GTPase rab4 was localized to early endosomes and numerous small vesicles in the cell periphery and Golgi area of PC12 cells. Overexpression of GTPase-deficient Q67Lrab4 increased the number of early endosome-associated and cytoplasmic vesicles, whereas expression of GDP-bound S22Nrab4 significantly increased the length of early endosomal tubules. In parallel, Q67Lrab4 induced a shift in rab4, VAMP2, and TfR label from early endosomes to peripheral vesicles, whereas S22Nrab4 increased early endosome labeling of all three proteins. These observations were corroborated by early endosome budding assays. Together, our data document a thus far unrecognized role for rab4 in the formation of synaptic-like microvesicles and add to our understanding of the formation of constitutive recycling vesicles from early endosomes.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
de Wit H,Lichtenstein Y,Kelly RB,Geuze HJ,Klumperman J,van der Sluijs Pdoi
10.1091/mbc.12.11.3703keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
3703-15issue
11eissn
1059-1524issn
1939-4586journal_volume
12pub_type
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