Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.

abstract：:In pancreatic β-cells, insulin granule membranes are enriched in cholesterol and are both recycled and newly generated. Cholesterol's role in supporting granule membrane formation and function is poorly understood. ATP binding cassette transporters ABCG1 and ABCA1 regulate intracellular cholesterol and are important f...

journal_title：Molecular biology of the cell

authors： Hussain SS,Harris MT,Kreutzberger AJB,Inouye CM,Doyle CA,Castle AM,Arvan P,Castle JD

更新日期：2018-05-15 00:00:00

abstract：:A heterozygous Caveolin-1 c.474delA mutation has been identified in a family with heritable pulmonary arterial hypertension (PAH). This frameshift mutation leads to caveolin-1 protein that contains all known functional domains but has a change only in the final 20 amino acids of the C terminus. Here we studied how thi...

journal_title：Molecular biology of the cell

authors： Marsboom G,Chen Z,Yuan Y,Zhang Y,Tiruppathi C,Loyd JE,Austin ED,Machado RF,Minshall RD,Rehman J,Malik AB

更新日期：2017-03-15 00:00:00

abstract：:In eukaryotic cells, organelle-specific protein quality control (PQC) is critical for maintaining cellular homeostasis. Despite the Golgi apparatus being the major protein processing and sorting site within the secretory pathway, how it contributes to PQC has remained largely unknown. Using different chemical biology-...

journal_title：Molecular biology of the cell

authors： Hellerschmied D,Serebrenik YV,Shao L,Burslem GM,Crews CM

更新日期：2019-08-01 00:00:00

abstract：:The [URE3] and [PSI(+)] prions are infectious amyloid forms of Ure2p and Sup35p. Several chaperones influence prion propagation: Hsp104p overproduction destabilizes [PSI(+)], whereas [URE3] is sensitive to excess of Ssa1p or Ydj1p. Here, we show that overproduction of the chaperone, Sse1p, can efficiently cure [URE3]....

journal_title：Molecular biology of the cell

authors： Kryndushkin D,Wickner RB

更新日期：2007-06-01 00:00:00

abstract：:Previous research has found that a gamma-tubulin mutation in Schizosaccharomyces pombe is synthetically lethal with a deletion of the C-terminal motor domain kinesin-like protein gene pkl1, but the lethality of the double mutant prevents a phenotypic analysis of the synthetic interaction. We have investigated interact...

journal_title：Molecular biology of the cell

authors： Prigozhina NL,Walker RA,Oakley CE,Oakley BR

更新日期：2001-10-01 00:00:00

abstract：:The export of mRNA from the nucleus to the cytoplasm involves interactions of proteins with mRNA and the nuclear pore complex. We isolated Crp79p, a novel mRNA export factor from the same synthetic lethal screen that led to the identification of spMex67p in Schizosaccharomyces pombe. Crp79p is a 710-amino-acid-long pr...

journal_title：Molecular biology of the cell

authors： Thakurta AG,Whalen WA,Yoon JH,Bharathi A,Kozak L,Whiteford C,Love DC,Hanover JA,Dhar R

更新日期：2002-08-01 00:00:00

abstract：:The cytoskeleton plays an important role in neuronal morphogenesis. We have identified and characterized a novel actin-binding protein, termed Mayven, predominantly expressed in brain. Mayven contains a BTB (broad complex, tramtrack, bric-a-brac)/POZ (poxvirus, zinc finger) domain-like structure in the predicted N ter...

journal_title：Molecular biology of the cell

authors： Soltysik-Espanola M,Rogers RA,Jiang S,Kim TA,Gaedigk R,White RA,Avraham H,Avraham S

更新日期：1999-07-01 00:00:00

abstract：:We recently determined the nucleotide sequence of the gene encoding p28, a light chain of inner dynein arms of Chlamydomonas axonemes. Here, we show that p28 is the protein encoded by the IDA4 locus. p28, and the dynein heavy chains normally associated with it, are completely absent from the flagella and cell bodies o...

journal_title：Molecular biology of the cell

authors： LeDizet M,Piperno G

更新日期：1995-06-01 00:00:00

abstract：:Polarized cell growth requires the coupling of a defined spatial site on the cell cortex to the apparatus that directs the establishment of cell polarity. In the budding yeast Saccharomyces cerevisiae, the Ras-family GTPase Rsr1p/Bud1p and its regulators select the proper site for bud emergence on the cell cortex. The...

journal_title：Molecular biology of the cell

authors： Kozminski KG,Beven L,Angerman E,Tong AH,Boone C,Park HO

更新日期：2003-12-01 00:00:00

abstract：:Select lipid-anchored proteins such as glycosylphosphatidylinositol (GPI)-anchored proteins and nonreceptor tyrosine kinases may preferentially partition into sphingomyelin-rich and cholesterol-rich plasmalemmal microdomains, thereby acquiring resistance to detergent extraction. Two such domains, caveolae and lipid ra...

journal_title：Molecular biology of the cell

authors： Oh P,Schnitzer JE

更新日期：2001-03-01 00:00:00

abstract：:The scaffold protein XB130 regulates cell growth, survival, and migration. Yeast two-hybrid screening suggests that XB130 interacts with another scaffold protein, Tks5. We hypothesized that XB130 and Tks5 form a macromolecular complex to mediate signal transduction cascades for the regulation of cell growth and surviv...

journal_title：Molecular biology of the cell

authors： Moodley S,Hui Bai X,Kapus A,Yang B,Liu M

更新日期：2015-12-01 00:00:00

abstract：:Cellular differentiation and lineage commitment are considered to be robust and irreversible processes during development. Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four transcription factors. We hypothesized that combinatorial expression of...

journal_title：Molecular biology of the cell

authors： Ishii R,Kami D,Toyoda M,Makino H,Gojo S,Ishii T,Umezawa A

更新日期：2012-09-01 00:00:00

abstract：:Cohesion establishment and maintenance are carried out by proteins that modify the activity of Cohesin, an essential complex that holds sister chromatids together. Constituents of the replication fork, such as the DNA polymerase alpha-binding protein Ctf4, contribute to cohesion in ways that are poorly understood. To ...

journal_title：Molecular biology of the cell

authors： Warren CD,Eckley DM,Lee MS,Hanna JS,Hughes A,Peyser B,Jie C,Irizarry R,Spencer FA

更新日期：2004-04-01 00:00:00

abstract：:Microtubules (MTs) have been proposed to act mechanically as compressive struts that resist both actomyosin contractile forces and their own polymerization forces to mechanically stabilize cell shape. To identify the origin of MT bending, we directly observed MT bending and F-actin transport dynamics in the periphery ...

journal_title：Molecular biology of the cell

authors： Bicek AD,Tüzel E,Demtchouk A,Uppalapati M,Hancock WO,Kroll DM,Odde DJ

更新日期：2009-06-01 00:00:00

abstract：:We show that a physiological role of the extensively studied cisternal Golgi rab protein, rab6, is modulation of Golgi apparatus response to stress. Taking exposure of cells to hypotonic media as the best-known example of mammalian Golgi stress response, we found that hypotonic-induced tubule extension from the Golgi ...

journal_title：Molecular biology of the cell

authors： Jiang S,Storrie B

更新日期：2005-05-01 00:00:00

abstract：:The Drosophila tumor suppressor protein lethal (2) giant larvae [l(2)gl] is involved in the establishment of epithelial cell polarity during development. Recently, a yeast homolog of the protein has been shown to interact with components of the post-Golgi exocytic machinery and to regulate a late step in protein secre...

journal_title：Molecular biology of the cell

authors： Müsch A,Cohen D,Yeaman C,Nelson WJ,Rodriguez-Boulan E,Brennwald PJ

更新日期：2002-01-01 00:00:00

abstract：:Methionine residues in proteins are susceptible to oxidation by reactive oxygen species, but can be repaired via reduction of the resulting methionine sulfoxides by methionine-S-sulfoxide reductase (MsrA) and methionine-R-sulfoxide reductase (MsrB). However, the identity of all methionine sulfoxide reductases involved...

journal_title：Molecular biology of the cell

authors： Kim HY,Gladyshev VN

更新日期：2004-03-01 00:00:00

abstract：:For several decades, the trans-Golgi network (TGN) was considered the most distal stop and hence the ultimate protein-sorting station for distinct apical and basolateral transport carriers that reach their respective surface domains in the direct trafficking pathway. However, recent reports of apical and basolateral c...

journal_title：Molecular biology of the cell

authors： Treyer A,Pujato M,Pechuan X,Müsch A

更新日期：2016-07-15 00:00:00

abstract：:Trends in increased tuberculosis infection and a fatality rate of approximately 23% have necessitated the search for alternative biomarkers using newly developed postgenomic approaches. Here we provide a systematic analysis of Mycobacterium tuberculosis (Mtb) by directly profiling its gene products. This analysis comb...

journal_title：Molecular biology of the cell

authors： Mawuenyega KG,Forst CV,Dobos KM,Belisle JT,Chen J,Bradbury EM,Bradbury AR,Chen X

更新日期：2005-01-01 00:00:00

abstract：:Metazoan cells rapidly exchange signals at tight cell-cell interfaces, including synapses and gap junctions. Advances in imaging recently exposed a third mode of intercellular cross-talk mediated by thin, actin-containing membrane extensions broadly known as "membrane" or "tunneling" nanotubes. An explosion of researc...

journal_title：Molecular biology of the cell

authors： Sherer NM

更新日期：2013-04-01 00:00:00

abstract：:The RNA component of the signal recognition particle (SRP) is universally required for cotranslational protein targeting. Biochemical studies have shown that SRP RNA participates in the central step of protein targeting by catalyzing the interaction of the SRP with the SRP receptor (SR). SRP RNA also accelerates GTP h...

journal_title：Molecular biology of the cell

authors： Bradshaw N,Walter P

更新日期：2007-07-01 00:00:00

abstract：:The spindle checkpoint prevents anaphase onset until all the chromosomes have successfully attached to the spindle microtubules. The mechanisms by which unattached kinetochores trigger and transmit a primary signal are poorly understood, although it seems to be dependent at least in part, on the kinetochore localizati...

journal_title：Molecular biology of the cell

authors： Vigneron S,Prieto S,Bernis C,Labbé JC,Castro A,Lorca T

更新日期：2004-10-01 00:00:00

abstract：:The gamma-tubulin small complex (gamma-TuSC) is an evolutionarily conserved heterotetramer essential for microtubule nucleation. We have determined the structure of the Saccharomyces cerevisiae gamma-TuSC at 25-A resolution by electron microscopy. gamma-TuSC is Y-shaped, with an elongated body connected to two arms. G...

journal_title：Molecular biology of the cell

authors： Kollman JM,Zelter A,Muller EG,Fox B,Rice LM,Davis TN,Agard DA

更新日期：2008-01-01 00:00:00

abstract：:How nuclear size is regulated is a fundamental cell-biological question with relevance to cancers, which often exhibit enlarged nuclei. We previously reported that conventional protein kinase C (cPKC) contributes to nuclear size reductions that occur during early Xenopus development. Here we report that PKC-mediated p...

journal_title：Molecular biology of the cell

authors： Edens LJ,Dilsaver MR,Levy DL

更新日期：2017-05-15 00:00:00

abstract：:The concept of critical concentration (CC) is central to understanding the behavior of microtubules (MTs) and other cytoskeletal polymers. Traditionally, these polymers are understood to have one CC, measured in multiple ways and assumed to be the subunit concentration necessary for polymer assembly. However, this fra...

journal_title：Molecular biology of the cell

authors： Jonasson EM,Mauro AJ,Li C,Labuz EC,Mahserejian SM,Scripture JP,Gregoretti IV,Alber M,Goodson HV

更新日期：2020-03-19 00:00:00

abstract：:We have studied telomere length in Schizosaccharomyces pombe strains carrying mutations affecting cell cycle checkpoints, DNA repair, and regulation of the Cdc2 protein kinase. Telomere shortening was found in rad1, rad3, rad17, and rad26 mutants. Telomere lengths in previously characterized rad1 mutants paralleled th...

journal_title：Molecular biology of the cell

authors： Dahlen M,Olsson T,Kanter-Smoler G,Ramne A,Sunnerhagen P

更新日期：1998-03-01 00:00:00

abstract：:An inducible fluorescent system based on GFP is presented that allows for the uncoupling of dendritic mRNA transport from subsequent protein synthesis at the single cell level. The iron-responsive element (IRE) derived from ferritin mRNA in the 5'-UTR of the GFP reporter mRNA renders translation of its mRNA dependent ...

journal_title：Molecular biology of the cell

authors： Macchi P,Hemraj I,Goetze B,Grunewald B,Mallardo M,Kiebler MA

更新日期：2003-04-01 00:00:00

abstract：:Specific changes in membrane lipid composition are implicated in actin cytoskeletal organization, vesicle formation, and control of cell polarity. Cdc50p, a membrane protein in the endosomal/trans-Golgi network compartments, is a noncatalytic subunit of Drs2p, which is implicated in translocation of phospholipids acro...

journal_title：Molecular biology of the cell

authors： Kishimoto T,Yamamoto T,Tanaka K

更新日期：2005-12-01 00:00:00

abstract：:This essay records a voyage of discovery from the "cradle of cell biology" to the present, focused on the biology of the oldest known cell organelle, the cilium. In the "romper room" of cilia and microtubule (MT) biology, the sliding MT hypothesis of ciliary motility was born. From the "summer of love," students and c...

journal_title：Molecular biology of the cell

authors： Satir P

更新日期：2014-11-01 00:00:00

abstract：:The function currently attributed to tetraspanins is to organize molecular complexes in the plasma membrane by using multiple cis-interactions. Additionally, the tetraspanin CD9 may be a receptor that binds the soluble ligand PSG17, a member of the immunoglobulin superfamily (IgSF)/CEA subfamily. However, previous dat...

journal_title：Molecular biology of the cell

authors： Ellerman DA,Ha C,Primakoff P,Myles DG,Dveksler GS

更新日期：2003-12-01 00:00:00