Interaction between a Ras and a Rho GTPase couples selection of a growth site to the development of cell polarity in yeast.

abstract：:The tomosyn/Sro7 family is thought to play an important role in cell surface trafficking both as an effector of Rab family GTPases and as a regulator of plasma-membrane SNARE function. Recent work has determined the binding site of GTP-bound Sec4 on Sro7. Here we examine the effect of mutations in Sro7 that block Sec4...

journal_title：Molecular biology of the cell

authors： Rossi G,Watson K,Kennedy W,Brennwald P

更新日期：2018-06-15 00:00:00

abstract：:Transforming growth factor-β (TGF-β) plays critical roles in numerous physiological and pathological responses. Cholesterol, a major plasma membrane component, can have pronounced effects on signaling responses. Cells continually monitor cholesterol content and activate multilayered transcriptional and translational s...

journal_title：Molecular biology of the cell

authors： Shapira KE,Ehrlich M,Henis YI

更新日期：2018-10-01 00:00:00

abstract：:Intestinal epithelial cells (IECs) exist in a metabolic state of low oxygen tension termed "physiologic hypoxia." An important factor in maintaining intestinal homeostasis is the transcription factor hypoxia-inducible factor (HIF), which is stabilized under hypoxic conditions and mediates IEC homeostatic responses to ...

journal_title：Molecular biology of the cell

authors： Dowdell AS,Cartwright IM,Goldberg MS,Kostelecky R,Ross T,Welch N,Glover LE,Colgan SP

更新日期：2020-09-15 00:00:00

abstract：:Yeast cells, when exposed to stress, can enter a protective state in which cell division, growth, and metabolism are down-regulated. They remain viable in this state until nutrients become available again. How cells enter this protective survival state and what happens at a cellular and subcellular level are largely u...

journal_title：Molecular biology of the cell

authors： Marini G,Nüske E,Leng W,Alberti S,Pigino G

更新日期：2020-06-01 00:00:00

abstract：:Unfertilized eggs and fertilized embryos from Drosophila mothers mutant for the plutonium (plu) gene contain giant polyploid nuclei resulting from unregulated S-phase. The PLU protein, a 19-kDa ankyrin repeat protein, is present in oocytes and early embryos but is not detectable after the completion of the initial rap...

journal_title：Molecular biology of the cell

authors： Elfring LK,Axton JM,Fenger DD,Page AW,Carminati JL,Orr-Weaver TL

更新日期：1997-04-01 00:00:00

abstract：:Junctional integrity of endothelial monolayers is crucial to control movement of molecules and cells across the endothelium. Examining the structure and dynamics of cell junctions in endothelial monolayers, we discovered a role for septins. Contacts between adjacent endothelial cells were dynamic, with protrusions ext...

journal_title：Molecular biology of the cell

authors： Kim J,Cooper JA

更新日期：2018-07-15 00:00:00

abstract：:In kinetochores, the Ndc80 complex couples the energy in a depolymerizing microtubule to perform the work of moving chromosomes. The complex directly binds microtubules using an unstructured, positively charged N-terminal tail located on Hec1/Ndc80. Hec1/Ndc80 also contains a calponin homology domain (CHD) that increa...

journal_title：Molecular biology of the cell

authors： Tooley JG,Miller SA,Stukenberg PT

更新日期：2011-04-15 00:00:00

abstract：:Clearance of misfolded proteins from the ER is central for maintenance of cellular homeostasis. This process requires coordinated recognition, ER-cytosol translocation, and finally ubiquitination-dependent proteasomal degradation. Here, we identify an ER resident seven-transmembrane protein (JAMP) that links ER chaper...

journal_title：Molecular biology of the cell

authors： Tcherpakov M,Broday L,Delaunay A,Kadoya T,Khurana A,Erdjument-Bromage H,Tempst P,Qiu XB,DeMartino GN,Ronai Z

更新日期：2008-11-01 00:00:00

abstract：:Cdc2 kinase activity is required for triggering entry into mitosis in all known eukaryotes. Elaborate mechanisms have evolved for regulating Cdc2 activity so that mitosis occurs in a timely manner, when preparations for its execution are complete. In Schizosaccharomyces pombe, Wee1 and a related Mik1 kinase are Cdc2-i...

journal_title：Molecular biology of the cell

authors： Campbell SD,Sprenger F,Edgar BA,O'Farrell PH

更新日期：1995-10-01 00:00:00

abstract：:In primary hepatocytes and HepG2 hepatoma cells, prolonged activation of the p42/44 mitogen-activated protein kinase (MAPK) pathway is associated with a reduction in DNA synthesis, mediated by increased expression of the cyclin-dependent kinase inhibitor protein p21 (Cip-1/WAF1/mda6) (p21). This study was performed to...

journal_title：Molecular biology of the cell

authors： Park JS,Qiao L,Gilfor D,Yang MY,Hylemon PB,Benz C,Darlington G,Firestone G,Fisher PB,Dent P

更新日期：2000-09-01 00:00:00

abstract：:Nuclear migration is required for normal development in both higher and lower eukaryotes. In fungi this process is mediated by cytoplasmic dynein. It is believed that this motor protein is anchored to the cell membrane and moves nuclei by capturing and pulling on spindle pole body microtubules. To date, four genes hav...

journal_title：Molecular biology of the cell

authors： Chiu YH,Xiang X,Dawe AL,Morris NR

更新日期：1997-09-01 00:00:00

abstract：:Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the clathrin adaptor complex AP-1 is involved in retroviral release. The absence of AP-1mu obtained by genetic knock-out or b...

journal_title：Molecular biology of the cell

authors： Camus G,Segura-Morales C,Molle D,Lopez-Vergès S,Begon-Pescia C,Cazevieille C,Schu P,Bertrand E,Berlioz-Torrent C,Basyuk E

更新日期：2007-08-01 00:00:00

abstract：:Saccharomyces cerevisiae mating pheromones induce production of Afr1p, a protein that negatively regulates pheromone receptor signaling and is required for normal formation of the projection of cell growth that becomes the site of cell fusion during conjugation. Afr1p interacts with Cdc12p, which belongs to a family o...

journal_title：Molecular biology of the cell

authors： Giot L,Konopka JB

更新日期：1997-06-01 00:00:00

abstract：:Motility, chemotaxis, and the acrosome reaction of animal sperm are all regulated by cyclic nucleotides and protein phosphorylation. One of the cyclic AMP-dependent protein kinase (PKA) substrates in sea urchin sperm is a member of the phosphodiesterase (PDE) family. The molecular identity and in vivo function of this...

journal_title：Molecular biology of the cell

authors： Su YH,Vacquier VD

更新日期：2006-01-01 00:00:00

abstract：:Biogenesis of respiratory chain complexes depends on the expression of mitochondrial-encoded subunits. Their synthesis occurs on membrane-associated ribosomes and is probably coupled to their membrane insertion. Defects in expression of mitochondrial translation products are among the major causes of mitochondrial dis...

journal_title：Molecular biology of the cell

authors： Bauerschmitt H,Mick DU,Deckers M,Vollmer C,Funes S,Kehrein K,Ott M,Rehling P,Herrmann JM

更新日期：2010-06-15 00:00:00

abstract：:Clathrin and caveolins are known for their involvement in the internalization of numerous receptors. Here we show that in polarized epithelial Madin-Darby canine kidney cells, both the clathrin machinery and caveolins are involved in the endocytosis and delivery to the plasma membrane (PM) of the M1 muscarinic acetylc...

journal_title：Molecular biology of the cell

authors： Shmuel M,Nodel-Berner E,Hyman T,Rouvinski A,Altschuler Y

更新日期：2007-05-01 00:00:00

abstract：:NuMA is an abundant long coiled-coil protein that plays a prominent role in spindle organization during mitosis. In interphase, NuMA is localized to the nucleus and hypothesized to control gene expression and chromatin organization. However, because of the prominent mitotic phenotype upon NuMA loss, its precise functi...

journal_title：Molecular biology of the cell

authors： Rajeevan A,Keshri R,Kapoor S,Kotak S

更新日期：2020-10-15 00:00:00

abstract：:Myosin Va is associated with discrete vesicle populations in a number of cell types, but little is known of the function of myosin Vb. Yeast two-hybrid screening of a rabbit parietal cell cDNA library with dominant active Rab11a (Rab11aS20V) identified myosin Vb as an interacting protein for Rab11a, a marker for plasm...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Kumar R,Hales CM,Navarre J,Bhartur SG,Burnette JO,Provance DW Jr,Mercer JA,Bähler M,Goldenring JR

更新日期：2001-06-01 00:00:00

abstract：:Many biological systems respond to environmental changes by activating intracellular signaling cascades, resulting in an appropriate response. One such system is represented by the social amoeba Dictyostelium discoideum. When food sources become scarce, these unicellular cells can initiate a cAMP-driven multicellular ...

journal_title：Molecular biology of the cell

authors： Vinet AF,Fiedler T,Studer V,Froquet R,Dardel A,Cosson P,Pieters J

更新日期：2014-03-01 00:00:00

abstract：:The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disru...

journal_title：Molecular biology of the cell

authors： Ahat E,Xiang Y,Zhang X,Bekier ME 2nd,Wang Y

更新日期：2019-03-15 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef directs virus escape from immune surveillance by subverting host cell intracellular signaling and membrane traffic to down-regulate cell-surface major histocompatibility complex class I (MHC-I). The interaction of Nef with the sorting proteins PACS-...

journal_title：Molecular biology of the cell

authors： Dikeakos JD,Thomas L,Kwon G,Elferich J,Shinde U,Thomas G

更新日期：2012-06-01 00:00:00

abstract：:Functionally different subsets of actin filament arrays contribute to cellular organization and motility. We report the identification of a novel subset of loose actin filament arrays through regulated association with the widely expressed protein SWAP-70. These loose actin filament arrays were commonly located behind...

journal_title：Molecular biology of the cell

authors： Hilpelä P,Oberbanscheidt P,Hahne P,Hund M,Kalhammer G,Small JV,Bähler M

更新日期：2003-08-01 00:00:00

abstract：:Due to extensive elaboration of the photoreceptor cilium to form the outer segment, axonemal transport (IFT) in photoreceptors is extraordinarily busy, and retinal degeneration is a component of many ciliopathies. Functional loss of heterotrimeric kinesin-2, a major anterograde IFT motor, causes mislocalized opsin, fo...

journal_title：Molecular biology of the cell

authors： Lopes VS,Jimeno D,Khanobdee K,Song X,Chen B,Nusinowitz S,Williams DS

更新日期：2010-12-01 00:00:00

abstract：:Cadherin-catenin interactions play an important role in cadherin-mediated adhesion. Here we present strong evidence that in the cadherin-catenin complex α-catenin contributes to the binding strength of another catenin, p120, to the same complex. Specifically, we found that a β-catenin-uncoupled cadherin mutant interac...

journal_title：Molecular biology of the cell

authors： Troyanovsky RB,Klingelhöfer J,Troyanovsky SM

更新日期：2011-11-01 00:00:00

abstract：:In addition to its central role in energy production, oxygen has pervasive regulatory actions. Hypoxia (oxygen limitation) triggers the shutdown of major cellular processes, including gene expression. We carried out a genome-wide RNA interference (RNAi) screen in Drosophila S2 cells for functions required to down-regu...

journal_title：Molecular biology of the cell

authors： Lee SJ,Feldman R,O'Farrell PH

更新日期：2008-10-01 00:00:00

abstract：:Candida albicans, the major human fungal pathogen, undergoes a reversible morphological transition from blastospores (round budding cells) to filaments (elongated cells attached end-to-end). This transition, which is induced upon exposure of C. albicans cells to a number of host conditions, including serum and body te...

journal_title：Molecular biology of the cell

authors： Kadosh D,Johnson AD

更新日期：2005-06-01 00:00:00

abstract：:Drosophila bristle cells are shaped during growth by longitudinal bundles of cross-linked actin filaments attached to the plasma membrane. We used confocal and electron microscopy to examine actin bundle structure and found that during bristle elongation, snarls of uncross-linked actin filaments and small internal bun...

journal_title：Molecular biology of the cell

authors： Tilney LG,Connelly PS,Ruggiero L,Vranich KA,Guild GM

更新日期：2003-10-01 00:00:00

abstract：:We examined the relative function of the two classes of guanine nucleotide exchange factors (GEFs) for ADP-ribosylation factors that regulate recruitment of coat proteins on the Golgi complex. Complementary overexpression and RNA-based knockdown approaches established that GBF1 regulates COPI recruitment on cis-Golgi ...

journal_title：Molecular biology of the cell

authors： Manolea F,Claude A,Chun J,Rosas J,Melançon P

更新日期：2008-02-01 00:00:00

abstract：:The kidney, together with bone and intestine, plays a crucial role in maintaining whole-body calcium (Ca(2+)) homoeostasis, which is primarily mediated by altering the reabsorption of Ca(2+) filtered by the glomerulus. The transient receptor potential-vanilloid-5 (TRPV5) channel protein forms a six- transmembrane Ca(2...

journal_title：Molecular biology of the cell

authors： Cai X,Srivastava S,Surindran S,Li Z,Skolnik EY

更新日期：2014-04-01 00:00:00

abstract：:The actin cytoskeleton is constantly assembling and disassembling. Cells harness the energy of these turnover dynamics to drive cell motility and organize cytoplasm. Although much is known about how cells control actin polymerization, we do not understand how actin filaments depolymerize inside cells. I briefly descri...

journal_title：Molecular biology of the cell

authors： Brieher W

更新日期：2013-08-01 00:00:00