XB130/Tks5 scaffold protein interaction regulates Src-mediated cell proliferation and survival.

abstract：:Disassembly of the epithelial apical junctional complex (AJC), composed of the tight junction (TJ) and adherens junction (AJ), is important for normal tissue remodeling and pathogen-induced disruption of epithelial barriers. Using a calcium depletion model in T84 epithelial cells, we previously found that disassembly ...

journal_title：Molecular biology of the cell

authors： Ivanov AI,McCall IC,Parkos CA,Nusrat A

更新日期：2004-06-01 00:00:00

abstract：:The tomosyn/Sro7 family is thought to play an important role in cell surface trafficking both as an effector of Rab family GTPases and as a regulator of plasma-membrane SNARE function. Recent work has determined the binding site of GTP-bound Sec4 on Sro7. Here we examine the effect of mutations in Sro7 that block Sec4...

journal_title：Molecular biology of the cell

authors： Rossi G,Watson K,Kennedy W,Brennwald P

更新日期：2018-06-15 00:00:00

abstract：:Dynamic regulation of cytoskeletal contractility through phosphorylation of the nonmuscle Myosin-II regulatory light chain (MRLC) provides an essential source of tension for shaping epithelial tissues. Rho GTPase and its effector kinase ROCK have been implicated in regulating MRLC phosphorylation in vivo, but evidence...

journal_title：Molecular biology of the cell

authors： Neubueser D,Hipfner DR

更新日期：2010-08-15 00:00:00

abstract：:The E3 ubiquitin ligase Rad18 chaperones DNA polymerase η (Polη) to sites of UV-induced DNA damage and monoubiquitinates proliferating cell nuclear antigen (PCNA), facilitating engagement of Polη with stalled replication forks and promoting translesion synthesis (TLS). It is unclear how Rad18 activities are coordinate...

journal_title：Molecular biology of the cell

authors： Barkley LR,Palle K,Durando M,Day TA,Gurkar A,Kakusho N,Li J,Masai H,Vaziri C

更新日期：2012-05-01 00:00:00

abstract：:The AP-1 transcription factor JunD is highly expressed in intestinal epithelial cells, but its exact role in maintaining the integrity of intestinal epithelial barrier remains unknown. The tight junction (TJ) protein zonula occludens (ZO)-1 links the intracellular domain of TJ-transmembrane proteins occludin, claudins...

journal_title：Molecular biology of the cell

authors： Chen J,Xiao L,Rao JN,Zou T,Liu L,Bellavance E,Gorospe M,Wang JY

更新日期：2008-09-01 00:00:00

abstract：:The organization of the endoplasmic reticulum (ER) in the cortex of Xenopus oocytes was investigated during maturation and activation using a green fluorescent protein chimera, immunofluorescence, and electron microscopy. Dense clusters of ER developed on the vegetal side (the side opposite the meiotic spindle) during...

journal_title：Molecular biology of the cell

authors： Terasaki M,Runft LL,Hand AR

更新日期：2001-04-01 00:00:00

abstract：:Isu, the scaffold protein on which Fe-S clusters are built in the mitochondrial matrix, plays a central role in the biogenesis of Fe-S cluster proteins. We report that the reduction in the activity of several components of the cluster biogenesis system, including the specialized Hsp70 Ssq1, causes a 15-20-fold up-regu...

journal_title：Molecular biology of the cell

authors： Andrew AJ,Song JY,Schilke B,Craig EA

更新日期：2008-12-01 00:00:00

abstract：:During a search for genes controlling conidial dormancy in Aspergillus fumigatus, two dehydrin-like genes, DprA and DprB, were identified. The deduced proteins had repeated stretches of 23 amino acids that contained a conserved dehydrin-like protein (DPR) motif. Disrupted DprAΔ mutants were hypersensitive to oxidative...

journal_title：Molecular biology of the cell

authors： Wong Sak Hoi J,Lamarre C,Beau R,Meneau I,Berepiki A,Barre A,Mellado E,Read ND,Latgé JP

更新日期：2011-06-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) is a key regulator of mitotic progression and cell division in eukaryotes. It is highly expressed in tumor cells and considered a potential target for cancer therapy. Here, we report the discovery and application of a novel potent small-molecule inhibitor of mammalian Plk1, ZK-Thiazolidinone ...

journal_title：Molecular biology of the cell

authors： Santamaria A,Neef R,Eberspächer U,Eis K,Husemann M,Mumberg D,Prechtl S,Schulze V,Siemeister G,Wortmann L,Barr FA,Nigg EA

更新日期：2007-10-01 00:00:00

abstract：:To test the "traction fiber" model for metaphase positioning of bivalents during meiosis, kinetochore fibers of maloriented bivalents, induced during recovery from cold arrest, were analyzed with a liquid crystal polarizing microscope. The measured birefringence retardation of kinetochore fibers is proportional to the...

journal_title：Molecular biology of the cell

authors： LaFountain JR Jr,Oldenbourg R

更新日期：2004-12-01 00:00:00

abstract：:ATP8A2 is a P4-ATPase that flips phosphatidylserine and phosphatidylethanolamine across cell membranes. This generates membrane phospholipid asymmetry, a property important in many cellular processes, including vesicle trafficking. ATP8A2 deficiency causes severe neurodegenerative diseases. We investigated the role of...

journal_title：Molecular biology of the cell

authors： Chalat M,Moleschi K,Molday RS

更新日期：2017-02-01 00:00:00

abstract：:Lumen establishment and maintenance are fundamental for tubular organs physiological functions. Most of the studies investigating the mechanisms regulating this process have been carried out in cell cultures or in smaller organisms, whereas little has been done in mammalian model systems in vivo. Here we used the sali...

journal_title：Molecular biology of the cell

authors： Shitara A,Malec L,Ebrahim S,Chen D,Bleck C,Hoffman MP,Weigert R

更新日期：2019-02-01 00:00:00

abstract：:The tumor suppressor protein p53 mediates stress-induced growth arrest or apoptosis and plays a major role in safeguarding genome integrity. In response to DNA damage, p53 can be modified at multiple sites by phosphorylation and acetylation. We report on the characterization of p53 C-terminal phosphorylation by CHK1 a...

journal_title：Molecular biology of the cell

authors： Ou YH,Chung PH,Sun TP,Shieh SY

更新日期：2005-04-01 00:00:00

abstract：:Protein degradation during the cell cycle is controlled by the opposing activities of ubiquitin ligases and deubiquitinating enzymes (DUBs). Although the functions of ubiquitin ligases in the cell cycle have been studied extensively, the roles of DUBs in this process are less well understood. Here, we used an overexpr...

journal_title：Molecular biology of the cell

authors： Mapa CE,Arsenault HE,Conti MM,Poti KE,Benanti JA

更新日期：2018-11-15 00:00:00

abstract：:The cdc25 tyrosine phosphatase is known to activate cdc2 kinase in the G2/M transition by dephosphorylation of tyrosine 15. To determine how entry into M-phase in eukaryotic cells is controlled, we have investigated the regulation of the cdc25 protein in Xenopus eggs and oocytes. Two closely related Xenopus cdc25 gene...

journal_title：Molecular biology of the cell

authors： Izumi T,Walker DH,Maller JL

更新日期：1992-08-01 00:00:00

abstract：:Biochemical data have shown that COPI-coated vesicles are tethered to Golgi membranes by a complex of at least three proteins: p115, giantin, and GM130. p115 binds to giantin on the vesicles and to GM130 on the membrane. We now examine the function of this tethering complex in vivo. Microinjection of an N-terminal pep...

journal_title：Molecular biology of the cell

authors： Seemann J,Jokitalo EJ,Warren G

更新日期：2000-02-01 00:00:00

abstract：:Cyclin D1 expression is jointly regulated by growth factors and cell adhesion to the extracellular matrix in many cell types. Growth factors are thought to regulate cyclin D1 expression because they stimulate sustained extracellular signal-regulated kinase (ERK) activity. However, we show here that growth factors indu...

journal_title：Molecular biology of the cell

authors： Roovers K,Davey G,Zhu X,Bottazzi ME,Assoian RK

更新日期：1999-10-01 00:00:00

abstract：:Regulator of G protein signaling (RGS) proteins inhibit G protein signaling by activating Galpha GTPase activity, but the mechanisms that regulate RGS activity are not well understood. The mammalian R7 binding protein (R7BP) can interact with all members of the R7 family of RGS proteins, and palmitoylation of R7BP can...

journal_title：Molecular biology of the cell

authors： Porter MY,Koelle MR

更新日期：2010-01-15 00:00:00

abstract：:Mathematical modeling has been instrumental in identifying common principles of cell polarity across diverse systems. These principles include positive feedback loops that are required to destabilize a spatially uniform state of the cell. The conserved small G-protein Cdc42 is a master regulator of eukaryotic cellular...

journal_title：Molecular biology of the cell

authors： Goryachev AB,Leda M

更新日期：2017-02-01 00:00:00

abstract：:Notch and neurotrophins control neuronal shape, but it is not known whether their signaling pathways intersect. Here we report results from hippocampal neuronal cultures that are in support of this possibility. We found that low cell density or blockade of Notch signaling by a soluble Delta-Fc ligand decreased the mRN...

journal_title：Molecular biology of the cell

authors： Salama-Cohen P,Arévalo MA,Meier J,Grantyn R,Rodríguez-Tébar A

更新日期：2005-01-01 00:00:00

abstract：:We recently established an in vitro assay that monitors the fusion between latex-bead phagosomes and endocytic organelles in the presence of J774 macrophage cytosol (). Here, we show that different reagents affecting the actin cytoskeleton can either inhibit or stimulate this fusion process. Because the membranes of p...

journal_title：Molecular biology of the cell

authors： Jahraus A,Egeberg M,Hinner B,Habermann A,Sackman E,Pralle A,Faulstich H,Rybin V,Defacque H,Griffiths G

更新日期：2001-01-01 00:00:00

abstract：:Alzheimer's β-amyloid precursor protein (APP) associates with kinesin-1 via JNK-interacting protein 1 (JIP1); however, the role of JIP1 in APP transport by kinesin-1 in neurons remains unclear. We performed a quantitative analysis to understand the role of JIP1 in APP axonal transport. In JIP1-deficient neurons, we fi...

journal_title：Molecular biology of the cell

authors： Chiba K,Araseki M,Nozawa K,Furukori K,Araki Y,Matsushima T,Nakaya T,Hata S,Saito Y,Uchida S,Okada Y,Nairn AC,Davis RJ,Yamamoto T,Kinjo M,Taru H,Suzuki T

更新日期：2014-11-05 00:00:00

abstract：:We report the identification of Cdc7/Dbf4 phosphorylation sites in human MCM2 and the determination of the role of Cdc7/Dbf4 phosphorylation of MCM2 in the initiation of DNA replication. Using immunoblotting, immunofluorescence, and high-speed automated cell-imaging analyses with antibodies specific against MCM2 and C...

journal_title：Molecular biology of the cell

authors： Tsuji T,Ficarro SB,Jiang W

更新日期：2006-10-01 00:00:00

abstract：:Retinol-binding protein (RBP) is secreted out of the cell in its ligand-bound holo-form. The apo-form of RBP is selectively retained within the endoplasmic reticulum (ER) by a mechanism that remains unknown. Using isolated microsomal system, we have recapitulated the biogenesis of RBP involving its oxidative folding a...

journal_title：Molecular biology of the cell

authors： Selvaraj SR,Bhatia V,Tatu U

更新日期：2008-12-01 00:00:00

abstract：:Understanding morphogenesis is the ultimate multidisciplinary (ad)venture. Three-dimensional tissues are generated from the actions of genes, biochemical pathways, and cells that form multicellular networks and interact with their biomechanical environment. A comprehensive explanation of morphogenetic processes must e...

journal_title：Molecular biology of the cell

authors： Zallen JA,Yap AS

更新日期：2010-03-15 00:00:00

abstract：:This essay records a voyage of discovery from the "cradle of cell biology" to the present, focused on the biology of the oldest known cell organelle, the cilium. In the "romper room" of cilia and microtubule (MT) biology, the sliding MT hypothesis of ciliary motility was born. From the "summer of love," students and c...

journal_title：Molecular biology of the cell

authors： Satir P

更新日期：2014-11-01 00:00:00

abstract：:A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of th...

journal_title：Molecular biology of the cell

authors： Midwood KS,Schwarzbauer JE

更新日期：2002-10-01 00:00:00

abstract：:Eukaryotic cells require IQGAP family multidomain adapter proteins for cytokinesis, but many questions remain about how IQGAPs contribute to the process. Here we show that fission yeast IQGAP Rng2p is required for both the normal process of contractile ring formation from precursor nodes and an alternative mechanism b...

journal_title：Molecular biology of the cell

authors： Tebbs IR,Pollard TD

更新日期：2013-06-01 00:00:00

abstract：:The Cdc42 guanosine triphosphatase (GTPase) plays a central role in polarity development in species ranging from yeast to humans. In budding yeast, a specific growth site is selected in the G1 phase. Rsr1, a Ras GTPase, interacts with Cdc42 and its associated proteins to promote polarized growth at the proper bud site...

journal_title：Molecular biology of the cell

authors： Miller KE,Lo WC,Chou CS,Park HO

更新日期：2019-09-15 00:00:00

abstract：:WDR92 is a highly conserved WD-repeat protein that has been proposed to be involved in apoptosis and also to be part of a prefoldin-like cochaperone complex. We found that WDR92 has a phylogenetic signature that is generally compatible with it playing a role in the assembly or function of specifically motile cilia. To...

journal_title：Molecular biology of the cell

authors： Patel-King RS,King SM

更新日期：2016-04-15 00:00:00