Abstract:
:The organization of the endoplasmic reticulum (ER) in the cortex of Xenopus oocytes was investigated during maturation and activation using a green fluorescent protein chimera, immunofluorescence, and electron microscopy. Dense clusters of ER developed on the vegetal side (the side opposite the meiotic spindle) during maturation. Small clusters appeared transiently at the time of nuclear envelope breakdown, disappeared at the time of first polar body formation, and then reappeared as larger clusters in mature eggs. The appearance of the large ER clusters was correlated with an increase in releasability of Ca(2+) by IP(3). The clusters dispersed during the Ca(2+) wave at activation. Possible relationships of ER structure and Ca(2+) regulation are discussed.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Terasaki M,Runft LL,Hand ARdoi
10.1091/mbc.12.4.1103keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
1103-16issue
4eissn
1059-1524issn
1939-4586journal_volume
12pub_type
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