Abstract:
:Rab GTPases are localized to various intracellular compartments and are known to play important regulatory roles in membrane trafficking. Here, we report the subcellular distribution and function of Rab14. By immunofluorescence and immunoelectron microscopy, both endogenous as well as overexpressed Rab14 were localized to biosynthetic (rough endoplasmic reticulum, Golgi, and trans-Golgi network) and endosomal compartments (early endosomal vacuoles and associated vesicles). Notably overexpression of Rab14Q70L shifted the distribution toward the early endosome associated vesicles, whereas the S25N and N124I mutants induced a shift toward the Golgi region. A similar, although less pronounced, redistribution of the transferrin receptor was also observed in cells overexpressing Rab14 mutants. Impairment of Rab14 function did not however affect transferrin uptake or recycling kinetics. Together, these findings suggest that Rab14 is involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Junutula JR,De Maziére AM,Peden AA,Ervin KE,Advani RJ,van Dijk SM,Klumperman J,Scheller RHdoi
10.1091/mbc.e03-10-0777keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
2218-29issue
5eissn
1059-1524issn
1939-4586pii
E03-10-0777journal_volume
15pub_type
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