Abstract:
:The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous and endogenous materials to lysosomes. However, the mechanisms that regulate these pathways are largely unknown. We previously reported that Rubicon, a Beclin 1-binding protein, negatively regulates both the autophagic and endocytic pathways by unidentified mechanisms. In this study, we performed database searches to identify potential Rubicon homologues that share the common C-terminal domain, termed the RH domain. One of them, PLEKHM1, the causative gene of osteopetrosis, also suppresses endocytic transport but not autophagosome maturation. Rubicon and PLEKHM1 specifically and directly interact with Rab7 via their RH domain, and this interaction is critical for their function. Furthermore, we show that Rubicon but not PLEKHM1 uniquely regulates membrane trafficking via simultaneously binding both Rab7 and PI3-kinase.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Tabata K,Matsunaga K,Sakane A,Sasaki T,Noda T,Yoshimori Tdoi
10.1091/mbc.E10-06-0495subject
Has Abstractpub_date
2010-12-01 00:00:00pages
4162-72issue
23eissn
1059-1524issn
1939-4586pii
E10-06-0495journal_volume
21pub_type
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