Intrinsic activity in cells and the brain.

abstract：:Motility, chemotaxis, and the acrosome reaction of animal sperm are all regulated by cyclic nucleotides and protein phosphorylation. One of the cyclic AMP-dependent protein kinase (PKA) substrates in sea urchin sperm is a member of the phosphodiesterase (PDE) family. The molecular identity and in vivo function of this...

journal_title：Molecular biology of the cell

authors： Su YH,Vacquier VD

更新日期：2006-01-01 00:00:00

abstract：:We have previously identified a novel family of proteins called the GGAs (Golgi-localized, gamma-ear-containing, ADP-ribosylation factor-binding proteins). These proteins consist of an NH(2)-terminal VHS domain, followed by a GAT domain, a variable domain, and a gamma-adaptin ear homology domain. Studies from our own ...

journal_title：Molecular biology of the cell

authors： Hirst J,Lindsay MR,Robinson MS

更新日期：2001-11-01 00:00:00

abstract：:The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disru...

journal_title：Molecular biology of the cell

authors： Ahat E,Xiang Y,Zhang X,Bekier ME 2nd,Wang Y

更新日期：2019-03-15 00:00:00

abstract：:The immunoglobulin (Ig) molecule is composed of two identical heavy chains and two identical light chains (H2L2). Transport of this heteromeric complex is dependent on the correct assembly of the component parts, which is controlled, in part, by the association of incompletely assembled Ig heavy chains with the endopl...

journal_title：Molecular biology of the cell

authors： Lee YK,Brewer JW,Hellman R,Hendershot LM

更新日期：1999-07-01 00:00:00

abstract：:GRIM-19 was found to copurify with complex I of mitochondrial respiratory chain and subsequently was demonstrated to be involved in complex I assembly and activity. To further understand its function in complex I, we dissected its functional domains by generating a number of deletion, truncation, and point mutants. Th...

journal_title：Molecular biology of the cell

authors： Lu H,Cao X

更新日期：2008-05-01 00:00:00

abstract：:We have recently shown that the pancreatic bile salt-dependent lipase (BSDL) can be taken up by intestinal cells and transported to the blood circulation. This mechanism likely involves (specific) receptor(s) able to bind BSDL and located at the apical intestinal cell membrane. In this study, using Int407 human intest...

journal_title：Molecular biology of the cell

authors： Bruneau N,Richard S,Silvy F,Verine A,Lombardo D

更新日期：2003-07-01 00:00:00

abstract：:We have shown previously that the transforming growth factor-beta (TGFbeta)-regulated Sma-Mad (Smad) protein 3 and Smad4 proteins transactivate the apolipoprotein C-III promoter in hepatic cells via a hormone response element that binds the nuclear receptor hepatocyte nuclear factor 4 (HNF-4). In the present study, we...

journal_title：Molecular biology of the cell

authors： Chou WC,Prokova V,Shiraishi K,Valcourt U,Moustakas A,Hadzopoulou-Cladaras M,Zannis VI,Kardassis D

更新日期：2003-03-01 00:00:00

abstract：:Starvation of Dictyostelium induces a developmental program in which cells form an aggregate that eventually differentiates into a multicellular structure. The aggregate formation is mediated by directional migration of individual cells that quickly transition to group migration in which cells align in a head-to-tail ...

journal_title：Molecular biology of the cell

authors： Garcia GL,Rericha EC,Heger CD,Goldsmith PK,Parent CA

更新日期：2009-07-01 00:00:00

abstract：:Actin is involved in endocytosis in organisms ranging from yeast to mammals. In activated Xenopus eggs, exocytosing cortical granules (CGs) are surrounded by actin "coats," which compress the exocytosing compartments, resulting in compensatory endocytosis. Here, we examined the roles of two myosins in actin coat compr...

journal_title：Molecular biology of the cell

authors： Yu HY,Bement WM

更新日期：2007-10-01 00:00:00

abstract：:Motor neuron (MN) diseases are progressive disorders resulting from degeneration of neuromuscular junctions (NMJs), which form the connection between MNs and muscle fibers. NMJ-in-a-dish models have been developed to examine human MN-associated dysfunction with disease; however such coculture models have randomly orie...

journal_title：Molecular biology of the cell

authors： Happe CL,Tenerelli KP,Gromova AK,Kolb F,Engler AJ

更新日期：2017-07-07 00:00:00

abstract：:In this study, we investigated the role of phospholipase D (PLD) in mediating Arf6 function in cells. Expression of Arf6 mutants that are defective in activating PLD, Arf6N48R and Arf6N48I, inhibited membrane recycling to the plasma membrane (PM), resulting in an accumulation of tubular endosomal membranes. Additional...

journal_title：Molecular biology of the cell

authors： Jovanovic OA,Brown FD,Donaldson JG

更新日期：2006-01-01 00:00:00

abstract：:We have used E1A probes to study the roles of the p34cdc2 kinase and the retinoblastoma tumor susceptibility gene product (pRB) in transforming growth factor beta 1 (TGF beta 1)-mediated growth suppression in mink lung epithelial (Mv1Lu) cells. In agreement with previous reports, we see a decline in p34cdc2 kinase act...

journal_title：Molecular biology of the cell

authors： Abraham SE,Carter MC,Moran E

更新日期：1992-06-01 00:00:00

abstract：:Epidermolysis bullosa simplex associated with late-onset muscular dystrophy (EBS-MD) is an autosomal recessive disorder resulting from mutations in the plectin gene. The majority of these mutations occur within the large exon 31 encoding the central rod domain and leave the production of a low-level rodless plectin sp...

journal_title：Molecular biology of the cell

authors： Ketema M,Secades P,Kreft M,Nahidiazar L,Janssen H,Jalink K,de Pereda JM,Sonnenberg A

更新日期：2015-07-01 00:00:00

abstract：:Cellular levels of the versatile second messenger cyclic (c)AMP are regulated by the antagonistic actions of the canonical G protein → adenylyl cyclase pathway that is initiated by G-protein-coupled receptors (GPCRs) and attenuated by phosphodiesterases (PDEs). Dysregulated cAMP signaling drives many diseases; for exa...

journal_title：Molecular biology of the cell

authors： Getz M,Swanson L,Sahoo D,Ghosh P,Rangamani P

更新日期：2019-06-15 00:00:00

abstract：:Drosophila myosin IB (Myo1B) is one of two class I myosins in the Drosophila genome. In the larval and adult midgut enterocyte, Myo1B is present within the microvillus (MV) of the apical brush border (BB) where it forms lateral tethers between the MV membrane and underlying actin filament core. Expression of green flu...

journal_title：Molecular biology of the cell

authors： Hegan PS,Mermall V,Tilney LG,Mooseker MS

更新日期：2007-11-01 00:00:00

abstract：:A disintegrin-like and metalloprotease with thrombospondin type I motif (ADAMTS9) is a member of the secreted metalloprotease family that is believed to digest extracellular matrix (ECM) proteins outside of cells. Its Caenorhabditis elegans orthologue, GON-1, is involved in ECM degradation and is required for gonad mo...

journal_title：Molecular biology of the cell

authors： Yoshina S,Sakaki K,Yonezumi-Hayashi A,Gengyo-Ando K,Inoue H,Iino Y,Mitani S

更新日期：2012-05-01 00:00:00

abstract：:The unfolded protein response (UPR) monitors and maintains protein homeostasis in the endoplasmic reticulum (ER). In budding yeast, the UPR is a transcriptional regulatory pathway that is quiescent under normal conditions. Under conditions of acute ER stress, activation of UPR targets is essential for cell viability. ...

journal_title：Molecular biology of the cell

authors： Hsu CL,Prasad R,Blackman C,Ng DT

更新日期：2012-02-01 00:00:00

abstract：:Mechanosensing describes the ability of a cell to sense mechanical cues of its microenvironment, including not only all components of force, stress, and strain but also substrate rigidity, topology, and adhesiveness. This ability is crucial for the cell to respond to the surrounding mechanical cues and adapt to the ch...

journal_title：Molecular biology of the cell

authors： Chen Y,Ju L,Rushdi M,Ge C,Zhu C

更新日期：2017-11-07 00:00:00

abstract：:The formation and disruption of synaptic connections during development are a fundamental step in neural circuit formation. Subneuronal structures such as neurites are known to be sensitive to the level of spontaneous neuronal activity, but the specifics of how neurotransmitter-induced calcium activity regulates neuri...

journal_title：Molecular biology of the cell

authors： Pearce KM,Bell M,Linthicum WH,Wen Q,Srinivasan J,Rangamani P,Scarlata S

更新日期：2020-03-19 00:00:00

abstract：:Cells have evolved an autoregulatory mechanism to dampen variations in the concentration of tubulin monomer that is available to polymerize into microtubules (MTs), a process that is known as tubulin autoregulation. However, thermodynamic analysis of MT polymerization predicts that the concentration of free tubulin mo...

journal_title：Molecular biology of the cell

authors： Mooney DJ,Hansen LK,Langer R,Vacanti JP,Ingber DE

更新日期：1994-12-01 00:00:00

abstract：:Two widely used hepatoma cell lines, mouse BW1J and human HepG2, express gene products characteristic of fetal hepatocytes, including serum albumin, whereas reporter genes driven by the albumin promoter are expressed at very low levels compared with highly differentiated hepatoma cells. We have investigated the low al...

journal_title：Molecular biology of the cell

authors： Rollier A,DiPersio CM,Cereghini S,Stevens K,Tronche F,Zaret K,Weiss MC

更新日期：1993-01-01 00:00:00

abstract：:Gaucher disease is a lysosomal storage disorder caused by a defect in the degradation of glucosylceramide catalyzed by the lysosomal enzyme β-glucocerebrosidase (GBA). GBA reaches lysosomes via association with its receptor, lysosomal integral membrane protein type 2 (LIMP-2). We found that distinct phosphatidylinosit...

journal_title：Molecular biology of the cell

authors： Jović M,Kean MJ,Szentpetery Z,Polevoy G,Gingras AC,Brill JA,Balla T

更新日期：2012-04-01 00:00:00

abstract：:We applied a combination of laser microsurgery and quantitative polarization microscopy to study kinetochore-independent forces that act on chromosome arms during meiosis in crane fly spermatocytes. When chromosome arms located within one of the half-spindles during prometa- or metaphase were cut with the laser, the a...

journal_title：Molecular biology of the cell

authors： LaFountain JR Jr,Oldenbourg R,Cole RW,Rieder CL

更新日期：2001-12-01 00:00:00

abstract：:CWH41, a gene involved in the assembly of cell wall beta-1,6-glucan, has recently been shown to be the structural gene for Saccharomyces cerevisiae glucosidase I that is responsible for initiating the trimming of terminal alpha-1,2-glucose residue in the N-glycan processing pathway. To distinguish between a direct or ...

journal_title：Molecular biology of the cell

authors： Abeijon C,Chen LY

更新日期：1998-10-01 00:00:00

abstract：:During organogenesis, different cell types need to work together to generate functional multicellular structures. To study this process, we made use of the genetically tractable fly retina, with a focus on the mechanisms that coordinate morphogenesis between the different epithelial cell types that make up the optical...

journal_title：Molecular biology of the cell

authors： Blackie L,Walther RF,Staddon MF,Banerjee S,Pichaud F

更新日期：2020-06-15 00:00:00

abstract：:The Rab11 effector Rab11-family interacting protein 2 (Rab11-FIP2) regulates transcytosis through its interactions with Rab11a and myosin Vb. Previous studies implicated Rab11-FIP2 in the establishment of polarity in Madin-Darby canine kidney (MDCK) cells through phosphorylation of Ser-227 by MARK2. Here we examine th...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Avant KM,Caldwell CM,Oztan A,Apodaca G,Knowles BC,Roland JT,Ducharme NA,Goldenring JR

更新日期：2012-06-01 00:00:00

abstract：:Cytoplasmic dynein is the major microtubule minus end-directed motor. Although studies have probed the mechanism of the C-terminal motor domain, if and how dynein's N-terminal tail and the accessory chains it binds regulate motor activity remain to be determined. Here, we investigate the structure and function of the ...

journal_title：Molecular biology of the cell

authors： Rao L,Romes EM,Nicholas MP,Brenner S,Tripathy A,Gennerich A,Slep KC

更新日期：2013-08-01 00:00:00

abstract：:Alternative pre-mRNA splicing patterns can change an extracellular stimulus, but the signaling pathways leading to these changes are still poorly characterized. Here, we describe a tyrosine-phosphorylated nuclear protein, YT521-B, and show that it interacts with the nuclear transcriptosomal component scaffold attachme...

journal_title：Molecular biology of the cell

authors： Hartmann AM,Nayler O,Schwaiger FW,Obermeier A,Stamm S

更新日期：1999-11-01 00:00:00

abstract：:Junctional integrity of endothelial monolayers is crucial to control movement of molecules and cells across the endothelium. Examining the structure and dynamics of cell junctions in endothelial monolayers, we discovered a role for septins. Contacts between adjacent endothelial cells were dynamic, with protrusions ext...

journal_title：Molecular biology of the cell

authors： Kim J,Cooper JA

更新日期：2018-07-15 00:00:00

abstract：:Type I interferons (IFN-alpha/beta) induce apoptosis in certain tumor cell lines but not others. Here we describe a mutation in STAT2 that confers an apoptotic effect in tumor cells in response to type I IFNs. This mutation was introduced in a conserved motif, PYTK, located in the STAT SH2 domain, which is shared by S...

journal_title：Molecular biology of the cell

authors： Scarzello AJ,Romero-Weaver AL,Maher SG,Veenstra TD,Zhou M,Qin A,Donnelly RP,Sheikh F,Gamero AM

更新日期：2007-07-01 00:00:00