Abstract:
:The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disrupts Golgi stack formation and impairs critical functions of the Golgi, such as accurate protein glycosylation and sorting. However, how Golgi destruction affects other cellular activities is so far unknown. Here, we report that depletion of GRASP proteins reduces cell attachment and migration. Interestingly, GRASP depletion reduces the protein level of α5β1 integrin, the major cell adhesion molecule at the surface of HeLa and MDA-MB-231 cells, due to decreased integrin protein synthesis. GRASP depletion also increases cell growth and total protein synthesis. These new findings enrich our understanding on the role of the Golgi in cell physiology and provide a potential target for treating protein-trafficking disorders.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Ahat E,Xiang Y,Zhang X,Bekier ME 2nd,Wang Ydoi
10.1091/mbc.E18-07-0462subject
Has Abstractpub_date
2019-03-15 00:00:00pages
766-777issue
6eissn
1059-1524issn
1939-4586journal_volume
30pub_type
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