Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa.

abstract：:Myosin II motors embedded within the actin cortex generate contractile forces to modulate cell shape in essential behaviors, including polarization, migration, and division. In sarcomeres, myosin II-mediated sliding of antiparallel F-actin is tightly coupled to myofibril contraction. By contrast, cortical F-actin is h...

journal_title：Molecular biology of the cell

authors： Murrell M,Gardel ML

更新日期：2014-06-15 00:00:00

abstract：:The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms in which 30-nm fibers have ...

journal_title：Molecular biology of the cell

authors： Cai S,Song Y,Chen C,Shi J,Gan L

更新日期：2018-07-01 00:00:00

abstract：:Triggering receptor expressed on myeloid cells 2 (TREM2) is a transmembrane protein expressed on microglia within the brain. Several rare mutations in TREM2 cause an early-onset form of neurodegeneration when inherited homozygously. Here we investigate how these mutations affect the intracellular transport of TREM2. W...

journal_title：Molecular biology of the cell

authors： Sirkis DW,Aparicio RE,Schekman R

更新日期：2017-10-01 00:00:00

abstract：:NSF and p97 are related AAA proteins implicated in membrane trafficking and organelle biogenesis. p97 is also involved in pathways that lead to ubiquitin-dependent proteolysis, including ER-associated degradation (ERAD). In this study, we have used dominant interfering ATP-hydrolysis deficient mutants (NSF(E329Q) and ...

journal_title：Molecular biology of the cell

authors： Dalal S,Rosser MF,Cyr DM,Hanson PI

更新日期：2004-02-01 00:00:00

abstract：:We have investigated the role of myosin in cytokinesis in Dictyostelium cells by examining cells under both adhesive and nonadhesive conditions. On an adhesive surface, both wild-type and myosin-null cells undergo the normal processes of mitotic rounding, cell elongation, polar ruffling, furrow ingression, and separat...

journal_title：Molecular biology of the cell

authors： Zang JH,Cavet G,Sabry JH,Wagner P,Moores SL,Spudich JA

更新日期：1997-12-01 00:00:00

abstract：:Fibroblasts, when plated on the extracellular matrix protein fibronectin (FN), rapidly spread and form an organized actin cytoskeleton. This process is known to involve both the central alpha5beta1 integrin-binding and the C-terminal heparin-binding regions of FN. We found that within the heparin-binding region, the i...

journal_title：Molecular biology of the cell

authors： Bloom L,Ingham KC,Hynes RO

更新日期：1999-05-01 00:00:00

abstract：:ER quality control (ERQC) prevents the exit of misfolded secretory and membrane proteins from the ER. A critical aspect of ERQC is a transcriptional response called the unfolded protein response (UPR), which up-regulates genes that enable cells to cope with misfolded, ER-retained proteins. In this study, we compare th...

journal_title：Molecular biology of the cell

authors： Metzger MB,Michaelis S

更新日期：2009-02-01 00:00:00

abstract：:Satellite cells (SCs), the resident adult stem cells of skeletal muscle, are required for tissue repair throughout life. While many signaling pathways are known to control SC self-renewal, less is known about the mechanisms underlying the spatiotemporal control of self-renewal during skeletal muscle repair. Here, we m...

journal_title：Molecular biology of the cell

authors： Moyle LA,Cheng RY,Liu H,Davoudi S,Ferreira SA,Nissar AA,Sun Y,Gentleman E,Simmons CA,Gilbert PM

更新日期：2020-07-21 00:00:00

abstract：:Ubiquitin-mediated proteolysis plays a key role in many pathways inside the cell and is particularly important in regulating cell cycle transitions. SCF (Skp1/Cul1/F-box protein) complexes are modular ubiquitin ligases whose specificity is determined by a substrate-binding F-box protein. Dia2 is a Saccharomyces cerevi...

journal_title：Molecular biology of the cell

authors： Koepp DM,Kile AC,Swaminathan S,Rodriguez-Rivera V

更新日期：2006-04-01 00:00:00

abstract：:The microphthalmia-associated transcription factor (MITF) is required for terminal osteoclast differentiation and is a target for signaling pathways engaged by colony stimulating factor (CSF)-1 and receptor-activator of nuclear factor-kappaB ligand (RANKL). Work presented here demonstrates that MITF can shuttle from c...

journal_title：Molecular biology of the cell

authors： Bronisz A,Sharma SM,Hu R,Godlewski J,Tzivion G,Mansky KC,Ostrowski MC

更新日期：2006-09-01 00:00:00

abstract：:Calcium (Ca(2+)) and calmodulin (CaM) are required for progression of mammalian cells from quiescence into S phase. In multiple cell types, cyclosporin A causes a G(1) cell cycle arrest, implicating the serine/threonine phosphatase calcineurin as one Ca(2+)/CaM-dependent enzyme required for G(1) transit. Here, we show...

journal_title：Molecular biology of the cell

authors： Kahl CR,Means AR

更新日期：2004-04-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, Cdc24p functions at least in part as a guanine-nucleotide-exchange factor for the Rho-family GTPase Cdc42p. A genetic screen designed to identify possible additional targets of Cdc24p instead identified two previously known genes, MSB1 and CLA4, and one novel gene, designated MSB...

journal_title：Molecular biology of the cell

authors： Bi E,Chiavetta JB,Chen H,Chen GC,Chan CS,Pringle JR

更新日期：2000-02-01 00:00:00

abstract：:Prostaglandins (PGs)--lipid signals produced downstream of cyclooxygenase (COX) enzymes--regulate actin dynamics in cell culture and platelets, but their roles during development are largely unknown. Here we define a new role for Pxt, the Drosophila COX-like enzyme, in regulating the actin cytoskeleton--temporal restr...

journal_title：Molecular biology of the cell

authors： Spracklen AJ,Kelpsch DJ,Chen X,Spracklen CN,Tootle TL

更新日期：2014-02-01 00:00:00

abstract：:Molecular chaperones play key roles during growth, development, and stress survival. The ability to induce chaperone expression enables cells to cope with the accumulation of nonnative proteins under stress and complete developmental processes with an increased requirement for chaperone assistance. Here we generate an...

journal_title：Molecular biology of the cell

authors： Rogon C,Ulbricht A,Hesse M,Alberti S,Vijayaraj P,Best D,Adams IR,Magin TM,Fleischmann BK,Höhfeld J

更新日期：2014-08-01 00:00:00

abstract：:Ghrelin is an acylated peptidyl gastric hormone acting on the pituitary and hypothalamus to stimulate appetite, adiposity, and growth hormone release, through activation of growth hormone secretagogue receptor (GHSR)-1a receptor. Moreover, ghrelin features several activities such as inhibition of apoptosis, regulation...

journal_title：Molecular biology of the cell

authors： Filigheddu N,Gnocchi VF,Coscia M,Cappelli M,Porporato PE,Taulli R,Traini S,Baldanzi G,Chianale F,Cutrupi S,Arnoletti E,Ghè C,Fubini A,Surico N,Sinigaglia F,Ponzetto C,Muccioli G,Crepaldi T,Graziani A

更新日期：2007-03-01 00:00:00

abstract：:Despite the importance of epithelial cell contacts in determining cell behavior, we still lack a detailed understanding of the assembly and disassembly of intercellular contacts. Here we examined the role of the catalytic activity of the Src family kinases at epithelial cell contacts in vitro. Like E- and P-cadherin, ...

journal_title：Molecular biology of the cell

authors： Owens DW,McLean GW,Wyke AW,Paraskeva C,Parkinson EK,Frame MC,Brunton VG

更新日期：2000-01-01 00:00:00

abstract：:Lactogenic hormones and extracellular matrix (ECM) act synergistically to regulate beta-casein expression in culture. We have developed a functional subpopulation of the mouse mammary epithelial cell strain COMMA-1D (designated CID 9), which expresses high level of beta-casein, forms alveolar-like structures when plat...

journal_title：Molecular biology of the cell

authors： Schmidhauser C,Casperson GF,Myers CA,Sanzo KT,Bolten S,Bissell MJ

更新日期：1992-06-01 00:00:00

abstract：:The efficient maintenance of genome integrity in the face of cellular stress is vital to protect against human diseases such as cancer. DNA replication, chromatin dynamics, cellular signaling, nuclear architecture, cell cycle checkpoints, and other cellular activities contribute to the delicate spatiotemporal control ...

journal_title：Molecular biology of the cell

authors： Jensen RB,Rothenberg E

更新日期：2020-04-15 00:00:00

abstract：:Dopamine (DA) increases Na(+),K(+)-ATPase activity in lung alveolar epithelial cells. This effect is associated with an increase in Na(+),K(+)-ATPase molecules within the plasma membrane (). Analysis of Na(+),K(+)-ATPase motion was performed in real-time in alveolar cells stably expressing Na(+),K(+)-ATPase molecules ...

journal_title：Molecular biology of the cell

authors： Bertorello AM,Komarova Y,Smith K,Leibiger IB,Efendiev R,Pedemonte CH,Borisy G,Sznajder JI

更新日期：2003-03-01 00:00:00

abstract：:Glycoprotein gp130, found on the plasma membrane of Dictyostelium discoideum amoebae, was postulated previously to play a role in phagocytosis. The gene for gp130 was cloned and when translated, yielded a 768 amino acid preproprotein of 85.3 kDa. It had nearly 40% similarity to the 138 kDa family of glycoproteins impl...

journal_title：Molecular biology of the cell

authors： Chia CP,Gomathinayagam S,Schmaltz RJ,Smoyer LK

更新日期：2005-06-01 00:00:00

abstract：:Although all microtubules within a single cell are polymerized from virtually identical subunits, different microtubule populations carry out specialized and diverse functions, including directional transport, force generation, and cellular morphogenesis. Functional differentiation requires specific targeting of assoc...

journal_title：Molecular biology of the cell

authors： Liu J,He Y,Benmerzouga I,Sullivan WJ Jr,Morrissette NS,Murray JM,Hu K

更新日期：2016-02-01 00:00:00

abstract：:Many integrin receptors localize to focal contact sites upon binding their ligand. However, unoccupied integrin receptors do not localize to focal contact sites. Because the integrin beta 1 cytoplasmic domain appears to have a focal contact localization signal, there must be a mechanism by which this domain is kept in...

journal_title：Molecular biology of the cell

authors： Briesewitz R,Kern A,Marcantonio EE

更新日期：1993-06-01 00:00:00

abstract：:Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). The anaphase-promoting complex or cyclosome (APC/C) comprises a multisubunit ubiquitin l...

journal_title：Molecular biology of the cell

authors： Leverson JD,Joazeiro CA,Page AM,Huang Hk,Hieter P,Hunter T

更新日期：2000-07-01 00:00:00

abstract：:Stimulation of the T-cell receptor (TCR) results in the activation of several transcription factors, including NF-kappaB, that are crucial for T-cell proliferation and gain of effector functions. On TCR engagement, several proteins within the TCR-directed NF-kappaB signaling pathway undergo dynamic spatial redistribut...

journal_title：Molecular biology of the cell

authors： Rossman JS,Stoicheva NG,Langel FD,Patterson GH,Lippincott-Schwartz J,Schaefer BC

更新日期：2006-05-01 00:00:00

abstract：:Drosophila myosin IB (Myo1B) is one of two class I myosins in the Drosophila genome. In the larval and adult midgut enterocyte, Myo1B is present within the microvillus (MV) of the apical brush border (BB) where it forms lateral tethers between the MV membrane and underlying actin filament core. Expression of green flu...

journal_title：Molecular biology of the cell

authors： Hegan PS,Mermall V,Tilney LG,Mooseker MS

更新日期：2007-11-01 00:00:00

abstract：:Yeast sulfur metabolism is transcriptionally regulated by the activator Met4. Met4 lacks DNA-binding ability and relies on interactions with Met31 and Met32, paralogous proteins that bind the same cis-regulatory element, to activate its targets. Although Met31 and Met32 are redundant for growth in the absence of methi...

journal_title：Molecular biology of the cell

authors： Carrillo E,Ben-Ari G,Wildenhain J,Tyers M,Grammentz D,Lee TA

更新日期：2012-05-01 00:00:00

abstract：:Mitogen-activated protein kinase phosphatase 2 (MKP2) is a member of the dual-specificity MKPs that regulate MAP kinase signaling. However, MKP2 functions are still largely unknown. In this study, we showed that MKP2 could regulate histone H3 phosphorylation under oxidative stress conditions. We found that MKP2 inhibi...

journal_title：Molecular biology of the cell

authors： Jeong MW,Kang TH,Kim W,Choi YH,Kim KT

更新日期：2013-02-01 00:00:00

abstract：:Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export. Here we show that Nab2 has additional roles during mRNA transcrip...

journal_title：Molecular biology of the cell

authors： González-Aguilera C,Tous C,Babiano R,de la Cruz J,Luna R,Aguilera A

更新日期：2011-08-01 00:00:00

abstract：:Biochemical data have shown that COPI-coated vesicles are tethered to Golgi membranes by a complex of at least three proteins: p115, giantin, and GM130. p115 binds to giantin on the vesicles and to GM130 on the membrane. We now examine the function of this tethering complex in vivo. Microinjection of an N-terminal pep...

journal_title：Molecular biology of the cell

authors： Seemann J,Jokitalo EJ,Warren G

更新日期：2000-02-01 00:00:00

abstract：:The Cvt pathway is a biosynthetic transport route for a distinct subset of resident yeast vacuolar hydrolases, whereas macroautophagy is a nonspecific degradative mechanism that allows cell survival during starvation. Yet, these two vacuolar trafficking pathways share a number of identical molecular components and are...

journal_title：Molecular biology of the cell

authors： Reggiori F,Wang CW,Nair U,Shintani T,Abeliovich H,Klionsky DJ

更新日期：2004-05-01 00:00:00