On the role of myosin-II in cytokinesis: division of Dictyostelium cells under adhesive and nonadhesive conditions.

abstract：:Repellents evoke growth cone turning by eliciting asymmetric, localized loss of actin cytoskeleton together with changes in substratum attachment. We have demonstrated that semaphorin-3A (Sema3A)-induced growth cone detachment and collapse require eicosanoid-mediated activation of protein kinase C epsilon (PKC epsilon...

journal_title：Molecular biology of the cell

authors： Gatlin JC,Estrada-Bernal A,Sanford SD,Pfenninger KH

更新日期：2006-12-01 00:00:00

abstract：:Methionine residues in proteins are susceptible to oxidation by reactive oxygen species, but can be repaired via reduction of the resulting methionine sulfoxides by methionine-S-sulfoxide reductase (MsrA) and methionine-R-sulfoxide reductase (MsrB). However, the identity of all methionine sulfoxide reductases involved...

journal_title：Molecular biology of the cell

authors： Kim HY,Gladyshev VN

更新日期：2004-03-01 00:00:00

abstract：:Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises from light excitation. An alternative is luciferase, an enzyme that ...

journal_title：Molecular biology of the cell

authors： Mazo-Vargas A,Park H,Aydin M,Buchler NE

更新日期：2014-11-05 00:00:00

abstract：:Clathrin and caveolins are known for their involvement in the internalization of numerous receptors. Here we show that in polarized epithelial Madin-Darby canine kidney cells, both the clathrin machinery and caveolins are involved in the endocytosis and delivery to the plasma membrane (PM) of the M1 muscarinic acetylc...

journal_title：Molecular biology of the cell

authors： Shmuel M,Nodel-Berner E,Hyman T,Rouvinski A,Altschuler Y

更新日期：2007-05-01 00:00:00

abstract：:Macrophages and dendritic cells exposed to lipopolysaccharide (LPS) convert their lysosomes from small, punctate organelles into a network of tubules. Tubular lysosomes have been implicated in phagosome maturation, retention of fluid phase, and antigen presentation. There is a growing appreciation that lysosomes act a...

journal_title：Molecular biology of the cell

authors： Saric A,Hipolito VE,Kay JG,Canton J,Antonescu CN,Botelho RJ

更新日期：2016-01-15 00:00:00

abstract：:In neurons certain mRNA transcripts are transported to synapses through mechanisms that are not fully understood. Here we report that the heterogeneous nuclear ribonucleoprotein CBF-A (CArG Box binding Factor A) facilitates dendritic transport and localization of activity-regulated cytoskeleton-associated protein (Arc...

journal_title：Molecular biology of the cell

authors： Raju CS,Fukuda N,López-Iglesias C,Göritz C,Visa N,Percipalle P

更新日期：2011-06-01 00:00:00

abstract：:Targeted endocytosis of plasma membrane (PM) proteins allows cells to adjust their complement of membrane proteins to changing extracellular conditions. For a wide variety of PM proteins, initiation of endocytosis is triggered by ubiquitination. In yeast, arrestin-related trafficking adaptors (ARTs) enable a single ub...

journal_title：Molecular biology of the cell

authors： Guiney EL,Klecker T,Emr SD

更新日期：2016-12-15 00:00:00

abstract：:Janus kinase 2 (Jak2) protein tyrosine kinase plays an important role in interleukin-3- or granulocyte-macrophage colony-stimulating factor-mediated signal transduction pathways leading to cell proliferation, activation of early response genes, and inhibition of apoptosis. However, it is unclear whether Jak2 can activ...

journal_title：Molecular biology of the cell

authors： Mohi MG,Arai Ki,Watanabe S

更新日期：1998-12-01 00:00:00

abstract：:Formins have conserved roles in cell polarity and cytokinesis and directly nucleate actin filament assembly through their FH2 domain. Here, we define the active region of the yeast formin Bni1 FH2 domain and show that it dimerizes. Mutations that disrupt dimerization abolish actin assembly activity, suggesting that di...

journal_title：Molecular biology of the cell

authors： Moseley JB,Sagot I,Manning AL,Xu Y,Eck MJ,Pellman D,Goode BL

更新日期：2004-02-01 00:00:00

abstract：:In addition to its central role in energy production, oxygen has pervasive regulatory actions. Hypoxia (oxygen limitation) triggers the shutdown of major cellular processes, including gene expression. We carried out a genome-wide RNA interference (RNAi) screen in Drosophila S2 cells for functions required to down-regu...

journal_title：Molecular biology of the cell

authors： Lee SJ,Feldman R,O'Farrell PH

更新日期：2008-10-01 00:00:00

abstract：:Transforming growth factor beta (TGF beta) inhibits cell proliferation by inducing a G1 cell-cycle arrest. Cyclin/CDK complexes have been implicated in this arrest, because TGF beta treatment leads to inhibition of cyclin/CDK activity. We have investigated the role of the retinoblastoma protein (pRb) in TGF beta-induc...

journal_title：Molecular biology of the cell

authors： Herrera RE,Mäkelä TP,Weinberg RA

更新日期：1996-09-01 00:00:00

abstract：:An inducible fluorescent system based on GFP is presented that allows for the uncoupling of dendritic mRNA transport from subsequent protein synthesis at the single cell level. The iron-responsive element (IRE) derived from ferritin mRNA in the 5'-UTR of the GFP reporter mRNA renders translation of its mRNA dependent ...

journal_title：Molecular biology of the cell

authors： Macchi P,Hemraj I,Goetze B,Grunewald B,Mallardo M,Kiebler MA

更新日期：2003-04-01 00:00:00

abstract：:During autophagy, double-membrane autophagosomes deliver sequestered cytoplasmic content to late endosomes and lysosomes for degradation. The molecular mechanism of autophagosome maturation is still poorly characterized. The small GTPase Rab11 regulates endosomal traffic and is thought to function at the level of recy...

journal_title：Molecular biology of the cell

authors： Szatmári Z,Kis V,Lippai M,Hegedus K,Faragó T,Lorincz P,Tanaka T,Juhász G,Sass M

更新日期：2014-02-01 00:00:00

abstract：:p38 alpha mitogen-activated protein (MAP) kinase is a broadly expressed signaling molecule that participates in the regulation of cellular responses to stress as well as in the control of proliferation and survival of many cell types. We have used cell lines derived from p38 alpha knockout mice to study the role of th...

journal_title：Molecular biology of the cell

authors： Porras A,Zuluaga S,Black E,Valladares A,Alvarez AM,Ambrosino C,Benito M,Nebreda AR

更新日期：2004-02-01 00:00:00

abstract：:Despite the fact that the chromosomal passenger complex is well known to regulate kinetochore behavior in mitosis, no functional link has yet been established between the complex and kinetochore structure. In addition, remarkably little is known about how the complex targets to centromeres. Here, in a study of caspase...

journal_title：Molecular biology of the cell

authors： Faragher AJ,Sun XM,Butterworth M,Harper N,Mulheran M,Ruchaud S,Earnshaw WC,Cohen GM

更新日期：2007-04-01 00:00:00

abstract：:The Rab GTPase Ypt11 is a Myo2-binding protein implicated in mother-to-bud transport of the cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during yeast division. However, its reported subcellular localization does not reflect all of these functions. Here we show that Ypt11 is normally a low-abundanc...

journal_title：Molecular biology of the cell

authors： Lewandowska A,Macfarlane J,Shaw JM

更新日期：2013-04-01 00:00:00

abstract：:We have identified and characterized sorb-1, the only sorbin and SH3 domain-containing protein family member in Caenorhabditis elegans SORB-1 is strongly localized to integrin adhesion complexes in larvae and adults, including adhesion plaques and dense bodies (Z-disks) of striated muscles and attachment plaques of sm...

journal_title：Molecular biology of the cell

authors： Loveless T,Qadota H,Benian GM,Hardin J

更新日期：2017-12-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the Apg12p-Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate with Apg5p in the apg7/cvt2 mutant. Apg7p shows a significant similarity to a ubiquitin-activating enzyme, Uba1p. In this a...

journal_title：Molecular biology of the cell

authors： Tanida I,Mizushima N,Kiyooka M,Ohsumi M,Ueno T,Ohsumi Y,Kominami E

更新日期：1999-05-01 00:00:00

abstract：:Due to extensive elaboration of the photoreceptor cilium to form the outer segment, axonemal transport (IFT) in photoreceptors is extraordinarily busy, and retinal degeneration is a component of many ciliopathies. Functional loss of heterotrimeric kinesin-2, a major anterograde IFT motor, causes mislocalized opsin, fo...

journal_title：Molecular biology of the cell

authors： Lopes VS,Jimeno D,Khanobdee K,Song X,Chen B,Nusinowitz S,Williams DS

更新日期：2010-12-01 00:00:00

abstract：:The trafficking of endocytosed receptors through phosphatidylinositol 3-phosphate [PtdIns(3)P]-containing endosomes is thought to attenuate their signaling. Here, we show that the PtdIns(3)P 5-kinase Fab1/PIKfyve controls trafficking but not silencing of endocytosed receptors. Drosophila fab1 mutants contain undetecta...

journal_title：Molecular biology of the cell

authors： Rusten TE,Rodahl LM,Pattni K,Englund C,Samakovlis C,Dove S,Brech A,Stenmark H

更新日期：2006-09-01 00:00:00

abstract：:Important aspects of cell cycle regulation are the checkpoints, which respond to a variety of cellular stresses to inhibit cell cycle progression and act as protective mechanisms to ensure genomic integrity. An increasing number of tumor suppressors are being demonstrated to have roles in checkpoint mechanisms, implyi...

journal_title：Molecular biology of the cell

authors： Qiu L,Burgess A,Fairlie DP,Leonard H,Parsons PG,Gabrielli BG

更新日期：2000-06-01 00:00:00

abstract：:ARL4D is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. Although the primary structure of ARL4D is very similar to that of other ARF/ARL molecules, its function remains unclear. Cytohesin-2/ARF nucleotide-binding-site opener (ARNO) is a guani...

journal_title：Molecular biology of the cell

authors： Li CC,Chiang TC,Wu TS,Pacheco-Rodriguez G,Moss J,Lee FJ

更新日期：2007-11-01 00:00:00

abstract：:Nuclear FGFR1 acts as a developmental gene regulator in cooperation with FGF-2, RSK1, and CREB-binding protein (CBP). FRAP analysis revealed three nuclear FGFR1 populations: i) a fast mobile, ii) a slower mobile population reflecting chromatin-bound FGFR1, and iii) an immobile FGFR1 population associated with the nucl...

journal_title：Molecular biology of the cell

authors： Dunham-Ems SM,Lee YW,Stachowiak EK,Pudavar H,Claus P,Prasad PN,Stachowiak MK

更新日期：2009-05-01 00:00:00

abstract：:Studies on physiological modulation of intercellular communication mediated by protein kinases are often complicated by the fact that cells express multiple gap junction proteins (connexins; Cx). Changes in cell coupling can be masked by simultaneous opposite regulation of the gap junction channel types expressed. We ...

journal_title：Molecular biology of the cell

authors： Kwak BR,Hermans MM,De Jonge HR,Lohmann SM,Jongsma HJ,Chanson M

更新日期：1995-12-01 00:00:00

abstract：:Guanine nucleotide exchange factors (GEFs) are the initiators of signaling by every regulatory GTPase, which in turn act to regulate a wide array of essential cellular processes. To date, each family of GTPases is activated by distinct families of GEFs. Bidirectional membrane trafficking is regulated by ADP-ribosylati...

journal_title：Molecular biology of the cell

authors： Pipaliya SV,Schlacht A,Klinger CM,Kahn RA,Dacks J

更新日期：2019-07-15 00:00:00

abstract：:Rho family proteins have been implicated in regulating various cellular processes, including actin cytoskeleton organization, endocytosis, cell cycle, and gene expression. In this study, we analyzed the function of a novel Dictyostelium discoideum Rho family protein (RacC). A cell line was generated that conditionally...

journal_title：Molecular biology of the cell

authors： Seastone DJ,Lee E,Bush J,Knecht D,Cardelli J

更新日期：1998-10-01 00:00:00

abstract：:Intracellular trafficking of tRNA was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. This view has recently been challenged, however, by the discovery that tRNA can move retrograde from the cytoplasm back to the nucleus in Saccharomyces cerev...

journal_title：Molecular biology of the cell

authors： Chafe SC,Pierce JB,Eswara MB,McGuire AT,Mangroo D

更新日期：2011-04-01 00:00:00

abstract：:Barrier to autointegration factor (BAF) is an essential component of the nuclear lamina that binds lamins, LEM-domain proteins, histones, and DNA. Under normal conditions, BAF protein is highly mobile when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching. We report that Cae...

journal_title：Molecular biology of the cell

authors： Bar DZ,Davidovich M,Lamm AT,Zer H,Wilson KL,Gruenbaum Y

更新日期：2014-04-01 00:00:00

abstract：:The ubiquitin-like hPLIC proteins can associate with proteasomes, and hPLIC overexpression can specifically interfere with ubiquitin-mediated proteolysis (Kleijnen et al., 2000). Because the hPLIC proteins can also interact with certain E3 ubiquitin protein ligases, they may provide a link between the ubiquitination a...

journal_title：Molecular biology of the cell

authors： Kleijnen MF,Alarcon RM,Howley PM

更新日期：2003-09-01 00:00:00

abstract：:Desmin interacts with nebulin establishing a direct link between the intermediate filament network and sarcomeres at the Z-discs. Here, we examined a desmin mutation, E245D, that is located within the coil IB (nebulin-binding) region of desmin and that has been reported to cause human cardiomyopathy and skeletal muscl...

journal_title：Molecular biology of the cell

authors： Conover GM,Henderson SN,Gregorio CC

更新日期：2009-02-01 00:00:00