Fab1 phosphatidylinositol 3-phosphate 5-kinase controls trafficking but not silencing of endocytosed receptors.

abstract：:Prostaglandins (PGs)--lipid signals produced downstream of cyclooxygenase (COX) enzymes--regulate actin dynamics in cell culture and platelets, but their roles during development are largely unknown. Here we define a new role for Pxt, the Drosophila COX-like enzyme, in regulating the actin cytoskeleton--temporal restr...

journal_title：Molecular biology of the cell

authors： Spracklen AJ,Kelpsch DJ,Chen X,Spracklen CN,Tootle TL

更新日期：2014-02-01 00:00:00

abstract：:The cell nucleus must continually resist and respond to intercellular and intracellular mechanical forces to transduce mechanical signals and maintain proper genome organization and expression. Altered nuclear mechanics is associated with many human diseases, including heart disease, progeria, and cancer. Chromatin an...

journal_title：Molecular biology of the cell

authors： Stephens AD,Banigan EJ,Adam SA,Goldman RD,Marko JF

更新日期：2017-07-07 00:00:00

abstract：:Focal adhesion kinase (FAK) is a cytoplasmic tyrosine kinase protein localized to regions called focal adhesions, which are contact points between cells and the extracellular matrix. FAK protein acts as a scaffold to transfer adhesion-dependent and growth factor signals into the cell. Increased FAK expression is linke...

journal_title：Molecular biology of the cell

authors： Fonar Y,Gutkovich YE,Root H,Malyarova A,Aamar E,Golubovskaya VM,Elias S,Elkouby YM,Frank D

更新日期：2011-07-01 00:00:00

abstract：:The proper positioning of mitotic spindle in the single-cell Caenorhabditis elegans embryo is achieved initially by the migration and rotation of the pronuclear complex (PNC) and its two associated astral microtubules (MTs). Pronuclear migration produces global cytoplasmic flows that couple the mechanics of all MTs, t...

journal_title：Molecular biology of the cell

authors： Nazockdast E,Rahimian A,Needleman D,Shelley M

更新日期：2017-11-07 00:00:00

abstract：:Sec1p/Munc18 (SM) family proteins regulate SNARE complex function in membrane fusion through their interactions with syntaxins. In addition to syntaxins, only a few SM protein interacting proteins are known and typically, their binding modes with SM proteins are poorly characterized. We previously identified Mso1p as ...

journal_title：Molecular biology of the cell

authors： Weber M,Chernov K,Turakainen H,Wohlfahrt G,Pajunen M,Savilahti H,Jäntti J

更新日期：2010-04-15 00:00:00

abstract：:Of the three structural maintenance of chromosomes (SMC) complexes, Smc5/6 remains the most poorly understood. Genetic studies have shown that Smc5/6 mutants are defective in homologous recombination (HR), and consistent with this, Smc5/6 is enriched at lesions. However, Smc5/6 is essential for viability, but HR is no...

journal_title：Molecular biology of the cell

authors： Tapia-Alveal C,O'Connell MJ

更新日期：2011-12-01 00:00:00

abstract：:Integrin alpha 3A cytoplasmic tail phosphorylation was mapped to amino acid S1042, as determined by mass spectrometry, and confirmed by mutagenesis. This residue occurs within a "QPSXXE" motif conserved in multiple alpha chains (alpha 3A, alpha 6A, alpha 7A), from multiple species. Phosphorylation of alpha 3A and alph...

journal_title：Molecular biology of the cell

authors： Zhang XA,Bontrager AL,Stipp CS,Kraeft SK,Bazzoni G,Chen LB,Hemler ME

更新日期：2001-02-01 00:00:00

abstract：:Regulation of Rho GTPase signaling is critical for cell shape determination and polarity. Here, we investigated the role of LRG1, a novel member of the GTPase-activating proteins (GAPs) of Neurospora crassa. LRG1 is essential for apical tip extension and to restrict excessive branch formation in subapical regions of t...

journal_title：Molecular biology of the cell

authors： Vogt N,Seiler S

更新日期：2008-11-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) is a key regulator of mitotic progression and cell division in eukaryotes. It is highly expressed in tumor cells and considered a potential target for cancer therapy. Here, we report the discovery and application of a novel potent small-molecule inhibitor of mammalian Plk1, ZK-Thiazolidinone ...

journal_title：Molecular biology of the cell

authors： Santamaria A,Neef R,Eberspächer U,Eis K,Husemann M,Mumberg D,Prechtl S,Schulze V,Siemeister G,Wortmann L,Barr FA,Nigg EA

更新日期：2007-10-01 00:00:00

abstract：:In addition to their well-known role in microtubule organization, centrosomes function as signaling platforms and regulate cell cycle events. An important example of such a function is the spindle position checkpoint (SPOC) of budding yeast. SPOC is a surveillance mechanism that ensures alignment of the mitotic spindl...

journal_title：Molecular biology of the cell

authors： Caydasi AK,Micoogullari Y,Kurtulmus B,Palani S,Pereira G

更新日期：2014-07-15 00:00:00

abstract：:Motility of cilia (also known as flagella in some eukaryotes) is based on axonemal doublet microtubule sliding that is driven by the dynein molecular motors. Dyneins are organized into intricately patterned inner and outer rows of arms, whose collective activity is to produce inter-microtubule movement. However, to ge...

journal_title：Molecular biology of the cell

authors： King SM,Sale WS

更新日期：2018-03-15 00:00:00

abstract：:EB1 is a conserved microtubule plus end tracking protein considered to play crucial roles in microtubule organization and the interaction of microtubules with the cell cortex. Despite intense studies carried out in yeast and cultured cells, the role of EB1 in multicellular systems remains to be elucidated. Here, we de...

journal_title：Molecular biology of the cell

authors： Elliott SL,Cullen CF,Wrobel N,Kernan MJ,Ohkura H

更新日期：2005-02-01 00:00:00

abstract：:During lactation, mammary epithelial cells secrete huge amounts of milk from their apical side. The current view is that caseins are secreted by exocytosis, whereas milk fat globules are released by budding, enwrapped by the plasma membrane. Owing to the number and large size of milk fat globules, the membrane surface...

journal_title：Molecular biology of the cell

authors： Honvo-Houéto E,Henry C,Chat S,Layani S,Truchet S

更新日期：2016-10-01 00:00:00

abstract：:The Rho GTPases RhoA and Rac1 function as master regulators of cytokinesis by controlling the actomyosin cytoskeleton. RhoA and Rac1 have to be respectively activated and inactivated at the division plane for cytokinesis to occur properly. The inactivation of Rac1 at the cleavage furrow is controlled by MgcRacGAP. How...

journal_title：Molecular biology of the cell

authors： Cannet A,Schmidt S,Delaval B,Debant A

更新日期：2014-12-15 00:00:00

abstract：:Triggering receptor expressed on myeloid cells 2 (TREM2) is a transmembrane protein expressed on microglia within the brain. Several rare mutations in TREM2 cause an early-onset form of neurodegeneration when inherited homozygously. Here we investigate how these mutations affect the intracellular transport of TREM2. W...

journal_title：Molecular biology of the cell

authors： Sirkis DW,Aparicio RE,Schekman R

更新日期：2017-10-01 00:00:00

abstract：:Desmin interacts with nebulin establishing a direct link between the intermediate filament network and sarcomeres at the Z-discs. Here, we examined a desmin mutation, E245D, that is located within the coil IB (nebulin-binding) region of desmin and that has been reported to cause human cardiomyopathy and skeletal muscl...

journal_title：Molecular biology of the cell

authors： Conover GM,Henderson SN,Gregorio CC

更新日期：2009-02-01 00:00:00

abstract：:Pre-rRNA synthesis and processing are key steps in ribosome biogenesis. Although recent evidence in yeast suggests that these two processes are coupled, the nature of their association is unclear. In this report, we analyze the coordination between rDNA transcription and pre-rRNA processing in mammalian cells. We foun...

journal_title：Molecular biology of the cell

authors： Kopp K,Gasiorowski JZ,Chen D,Gilmore R,Norton JT,Wang C,Leary DJ,Chan EK,Dean DA,Huang S

更新日期：2007-02-01 00:00:00

abstract：:The TAM receptor protein tyrosine kinases Tyro3, Axl, and Mer play important roles in macrophage function. We investigated the roles of the TAM receptors in mediating the induction of hepatocyte growth factor (HGF) during the interaction of macrophages with apoptotic cells. Mer-specific neutralizing antibody, small in...

journal_title：Molecular biology of the cell

authors： Park HJ,Baen JY,Lee YJ,Choi YH,Kang JL

更新日期：2012-08-01 00:00:00

abstract：:Hsk1, Saccharomyces cerevisiae Cdc7-related kinase in Shizosaccharomyces pombe, is required for G1/S transition and its kinase activity is controlled by the regulatory subunit Dfp1/Him1. Analyses of a newly isolated temperature-sensitive mutant, hsk1-89, reveal that Hsk1 plays crucial roles in DNA replication checkpoi...

journal_title：Molecular biology of the cell

authors： Takeda T,Ogino K,Tatebayashi K,Ikeda H,Arai Ki,Masai H

更新日期：2001-05-01 00:00:00

abstract：:Tolerance to desiccation in cultures of Saccharomyces cerevisiae is inducible; only one in a million cells from an exponential culture survive desiccation compared with one in five cells in stationary phase. Here we exploit the desiccation sensitivity of exponentially dividing cells to understand the stresses imposed ...

journal_title：Molecular biology of the cell

authors： Welch AZ,Gibney PA,Botstein D,Koshland DE

更新日期：2013-01-01 00:00:00

abstract：:Polyadenylation-induced translation is an important regulatory mechanism during metazoan development. During Xenopus oocyte meiotic progression, polyadenylation-induced translation is regulated by CPEB, which is activated by phosphorylation. XGef, a guanine exchange factor, is a CPEB-interacting protein involved in th...

journal_title：Molecular biology of the cell

authors： Martínez SE,Yuan L,Lacza C,Ransom H,Mahon GM,Whitehead IP,Hake LE

更新日期：2005-03-01 00:00:00

abstract：:Microtubules (MTs) in newt mitotic spindles grow faster than MTs in the interphase cytoplasmic microtubule complex (CMTC), yet spindle MTs do not have the long lengths or lifetimes of the CMTC microtubules. Because MTs undergo dynamic instability, it is likely that changes in the durations of growth or shortening are ...

journal_title：Molecular biology of the cell

authors： Gliksman NR,Skibbens RV,Salmon ED

更新日期：1993-10-01 00:00:00

abstract：:The late Golgi of the yeast Saccharomyces cerevisiae receives membrane traffic from the secretory pathway as well as retrograde traffic from post-Golgi compartments, but the machinery that regulates these vesicle-docking and fusion events has not been characterized. We have identified three components of a novel prote...

journal_title：Molecular biology of the cell

authors： Conibear E,Stevens TH

更新日期：2000-01-01 00:00:00

abstract：:Many mitochondrial outer membrane (MOM) proteins have a transmembrane domain near the C terminus and an N-terminal cytosolic moiety. It is not clear how these tail-anchored (TA) proteins posttranslationally select their target, but C-terminal charged residues play an important role. To investigate how discrimination b...

journal_title：Molecular biology of the cell

authors： Borgese N,Gazzoni I,Barberi M,Colombo S,Pedrazzini E

更新日期：2001-08-01 00:00:00

abstract：:The serotonin (5-hydroxytryptamine; 5-HT)(2C) receptor is a G protein-coupled receptor (GPCR) exclusively expressed in CNS that has been implicated in numerous brain disorders, including anxio-depressive states. Like many GPCRs, 5-HT(2C) receptors physically interact with a variety of intracellular proteins in additio...

journal_title：Molecular biology of the cell

authors： Labasque M,Reiter E,Becamel C,Bockaert J,Marin P

更新日期：2008-11-01 00:00:00

abstract：:Rab 11 GTPase is an important regulator of endocytic membrane traffic. Recently, we and others have identified a novel family of Rab11 binding proteins, known as Rab11-family interacting proteins (FIPs). One of the family members, Rab coupling protein (RCP), was identified as a protein binding to both Rab4 and Rab11 G...

journal_title：Molecular biology of the cell

authors： Peden AA,Schonteich E,Chun J,Junutula JR,Scheller RH,Prekeris R

更新日期：2004-08-01 00:00:00

abstract：:Disassembly of the epithelial apical junctional complex (AJC), composed of the tight junction (TJ) and adherens junction (AJ), is important for normal tissue remodeling and pathogen-induced disruption of epithelial barriers. Using a calcium depletion model in T84 epithelial cells, we previously found that disassembly ...

journal_title：Molecular biology of the cell

authors： Ivanov AI,McCall IC,Parkos CA,Nusrat A

更新日期：2004-06-01 00:00:00

abstract：:Mathematical modeling has been instrumental in identifying common principles of cell polarity across diverse systems. These principles include positive feedback loops that are required to destabilize a spatially uniform state of the cell. The conserved small G-protein Cdc42 is a master regulator of eukaryotic cellular...

journal_title：Molecular biology of the cell

authors： Goryachev AB,Leda M

更新日期：2017-02-01 00:00:00

abstract：:In animal cells, positioning of the mitotic spindle is crucial for defining the plane of cytokinesis and the size ratio of daughter cells. We have characterized this phenomenon in a rat epithelial cell line using microscopy, micromanipulation, and microinjection. Unmanipulated cells position the mitotic spindle near t...

journal_title：Molecular biology of the cell

authors： O'Connell CB,Wang YL

更新日期：2000-05-01 00:00:00

abstract：:The nuclear import receptors importin β and transportin play a different role in mitosis: both act phenotypically as spatial regulators to ensure that mitotic spindle, nuclear membrane, and nuclear pore assembly occur exclusively around chromatin. Importin β is known to act by repressing assembly factors in regions di...

journal_title：Molecular biology of the cell

authors： Bernis C,Swift-Taylor B,Nord M,Carmona S,Chook YM,Forbes DJ

更新日期：2014-04-01 00:00:00