Abstract:
:Barrier to autointegration factor (BAF) is an essential component of the nuclear lamina that binds lamins, LEM-domain proteins, histones, and DNA. Under normal conditions, BAF protein is highly mobile when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching. We report that Caenorhabditis elegans BAF-1 mobility is regulated by caloric restriction, food deprivation, and heat shock. This was not a general response of chromatin-associated proteins, as food deprivation did not affect the mobility of heterochromatin protein HPL-1 or HPL-2. Heat shock also increased the level of BAF-1 Ser-4 phosphorylation. By using missense mutations that affect BAF-1 binding to different partners we find that, overall, the ability of BAF-1 mutants to be immobilized by heat shock in intestinal cells correlated with normal or increased affinity for emerin in vitro. These results show BAF-1 localization and mobility at the nuclear lamina are regulated by stress and unexpectedly reveal BAF-1 immobilization as a specific response to caloric restriction in C. elegans intestinal cells.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Bar DZ,Davidovich M,Lamm AT,Zer H,Wilson KL,Gruenbaum Ydoi
10.1091/mbc.E13-08-0477subject
Has Abstractpub_date
2014-04-01 00:00:00pages
1127-36issue
7eissn
1059-1524issn
1939-4586pii
mbc.E13-08-0477journal_volume
25pub_type
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