Tight function zonula occludens-3 regulates cyclin D1-dependent cell proliferation.

abstract：:Clathrin-mediated endocytosis is a fundamental cellular process conserved from yeast to mammals and is an important endocytic route for the internalization of many specific cargos, including activated growth factor receptors. Here we examined changes in tyrosine phosphorylation, a representative output of growth facto...

journal_title：Molecular biology of the cell

authors： Shen H,Ferguson SM,Dephoure N,Park R,Yang Y,Volpicelli-Daley L,Gygi S,Schlessinger J,De Camilli P

更新日期：2011-02-15 00:00:00

abstract：:Human HT2-19 cells with a conditional cdk1 mutation stop dividing upon cdk1 inactivation and undergo multiple rounds of endoreplication. We show herein that major cell cycle events remain synchronized in these endoreplicating cells. DNA replication alternates with gap phases and cell cycle-specific cyclin E expression...

journal_title：Molecular biology of the cell

authors： Laronne A,Rotkopf S,Hellman A,Gruenbaum Y,Porter AC,Brandeis M

更新日期：2003-09-01 00:00:00

abstract：:Clathrin-mediated endocytosis and phagocytosis are both selective surface internalization processes but have little known mechanistic similarity or interdependence. Here we show that the phosphotyrosine-binding (PTB) domain protein Ced-6, a well-established phagocytosis component that operates as a transducer of so-ca...

journal_title：Molecular biology of the cell

authors： Jha A,Watkins SC,Traub LM

更新日期：2012-05-01 00:00:00

abstract：:Kinetochores are the proteinaceous complexes that assemble on centromeric DNA and direct eukaryotic chromosome segregation. The mechanisms by which higher eukaryotic cells define centromeres are poorly understood. Possible molecular contributors to centromere specification include the underlying DNA sequences and epig...

journal_title：Molecular biology of the cell

authors： Edwards NS,Murray AW

更新日期：2005-04-01 00:00:00

abstract：:Fibroblasts, when plated on the extracellular matrix protein fibronectin (FN), rapidly spread and form an organized actin cytoskeleton. This process is known to involve both the central alpha5beta1 integrin-binding and the C-terminal heparin-binding regions of FN. We found that within the heparin-binding region, the i...

journal_title：Molecular biology of the cell

authors： Bloom L,Ingham KC,Hynes RO

更新日期：1999-05-01 00:00:00

abstract：:Dog thyroid epithelial cells in primary culture constitute a physiologically relevant model of positive control of DNA synthesis initiation and G0-S prereplicative phase progression by cAMP as a second messenger for thyrotropin (thyroid-stimulating hormone [TSH]). As previously shown in this system, the cAMP-dependent...

journal_title：Molecular biology of the cell

authors： Depoortere F,Pirson I,Bartek J,Dumont JE,Roger PP

更新日期：2000-03-01 00:00:00

abstract：:Actin is involved in endocytosis in organisms ranging from yeast to mammals. In activated Xenopus eggs, exocytosing cortical granules (CGs) are surrounded by actin "coats," which compress the exocytosing compartments, resulting in compensatory endocytosis. Here, we examined the roles of two myosins in actin coat compr...

journal_title：Molecular biology of the cell

authors： Yu HY,Bement WM

更新日期：2007-10-01 00:00:00

abstract：:Rab GTPases are localized to various intracellular compartments and are known to play important regulatory roles in membrane trafficking. Here, we report the subcellular distribution and function of Rab14. By immunofluorescence and immunoelectron microscopy, both endogenous as well as overexpressed Rab14 were localize...

journal_title：Molecular biology of the cell

authors： Junutula JR,De Maziére AM,Peden AA,Ervin KE,Advani RJ,van Dijk SM,Klumperman J,Scheller RH

更新日期：2004-05-01 00:00:00

abstract：:Hedgehog (Hh) is a secreted morphogen involved in both short- and long-range signaling necessary for tissue patterning during development. It is unclear how this dually lipidated protein is transported over a long range in the aqueous milieu of interstitial spaces. We previously showed that the long-range signaling of...

journal_title：Molecular biology of the cell

authors： Parchure A,Vyas N,Ferguson C,Parton RG,Mayor S

更新日期：2015-12-15 00:00:00

abstract：:Telomere dysfunction results in fertility defects in a number of organisms. Although data from fission yeast and Caenorhabditis elegans suggests that telomere dysfunction manifests itself primarily as defects in proper meiotic chromosome segregation, it is unclear how mammalian telomere dysfunction results in germ cel...

journal_title：Molecular biology of the cell

authors： Hemann MT,Rudolph KL,Strong MA,DePinho RA,Chin L,Greider CW

更新日期：2001-07-01 00:00:00

abstract：:Substrates critical for transformation by pp60v-src remain unknown, as does the precise role of the src homology 2 (SH2) domain in this process. To continue exploring the role of the SH2 domain in pp60v-src-mediated transformation, site-directed mutagenesis was used to create mutant v-src alleles predicted to encode p...

journal_title：Molecular biology of the cell

authors： Verderame MF

更新日期：1997-05-01 00:00:00

abstract：:A role of Arp2/3 complex in lamellipodia is well established, whereas its roles in filopodia formation remain obscure. We addressed this question in neuronal cells, in which motility is heavily based on filopodia, and we found that Arp2/3 complex is involved in generation of both lamellipodia and filopodia in growth c...

journal_title：Molecular biology of the cell

authors： Korobova F,Svitkina T

更新日期：2008-04-01 00:00:00

abstract：:Satellite cells (SCs), the resident adult stem cells of skeletal muscle, are required for tissue repair throughout life. While many signaling pathways are known to control SC self-renewal, less is known about the mechanisms underlying the spatiotemporal control of self-renewal during skeletal muscle repair. Here, we m...

journal_title：Molecular biology of the cell

authors： Moyle LA,Cheng RY,Liu H,Davoudi S,Ferreira SA,Nissar AA,Sun Y,Gentleman E,Simmons CA,Gilbert PM

更新日期：2020-07-21 00:00:00

abstract：:We have shown previously that the transforming growth factor-beta (TGFbeta)-regulated Sma-Mad (Smad) protein 3 and Smad4 proteins transactivate the apolipoprotein C-III promoter in hepatic cells via a hormone response element that binds the nuclear receptor hepatocyte nuclear factor 4 (HNF-4). In the present study, we...

journal_title：Molecular biology of the cell

authors： Chou WC,Prokova V,Shiraishi K,Valcourt U,Moustakas A,Hadzopoulou-Cladaras M,Zannis VI,Kardassis D

更新日期：2003-03-01 00:00:00

abstract：:The split-ubiquitin technique was used to detect transient protein interactions in living cells. Nub, the N-terminal half of ubiquitin (Ub), was fused to Sec62p, a component of the protein translocation machinery in the endoplasmic reticulum of Saccharomyces cerevisiae. Cub, the C-terminal half of Ub, was fused to the...

journal_title：Molecular biology of the cell

authors： Dünnwald M,Varshavsky A,Johnsson N

更新日期：1999-02-01 00:00:00

abstract：:The intracellular trafficking of the epidermal growth factor receptor (EGFR) is regulated by a cross-talk between calmodulin (CaM) and protein kinase Cdelta (PKCdelta). On inhibition of CaM, PKCdelta promotes the formation of enlarged early endosomes and blocks EGFR recycling and degradation. Here, we show that PKCdel...

journal_title：Molecular biology of the cell

authors： Lladó A,Timpson P,Vilà de Muga S,Moretó J,Pol A,Grewal T,Daly RJ,Enrich C,Tebar F

更新日期：2008-01-01 00:00:00

abstract：:In vertebrates, zyxin is a LIM-domain protein belonging to a family composed of seven members. We show that the nematode Caenorhabditis elegans has a unique zyxin-like protein, ZYX-1, which is the orthologue of the vertebrate zyxin subfamily composed of zyxin, migfilin, TRIP6, and LPP. The ZYX-1 protein is expressed i...

journal_title：Molecular biology of the cell

authors： Lecroisey C,Brouilly N,Qadota H,Mariol MC,Rochette NC,Martin E,Benian GM,Ségalat L,Mounier N,Gieseler K

更新日期：2013-04-01 00:00:00

abstract：:S100A4, a member of the S100 family of Ca(2+)-binding proteins, is directly involved in tumor metastasis. In addition to its expression in tumor cells, S100A4 is expressed in normal cells and tissues, including fibroblasts and cells of the immune system. To examine the contribution of S100A4 to normal physiology, we e...

journal_title：Molecular biology of the cell

authors： Li ZH,Dulyaninova NG,House RP,Almo SC,Bresnick AR

更新日期：2010-08-01 00:00:00

abstract：:Triggering receptor expressed on myeloid cells 2 (TREM2) is a transmembrane protein expressed on microglia within the brain. Several rare mutations in TREM2 cause an early-onset form of neurodegeneration when inherited homozygously. Here we investigate how these mutations affect the intracellular transport of TREM2. W...

journal_title：Molecular biology of the cell

authors： Sirkis DW,Aparicio RE,Schekman R

更新日期：2017-10-01 00:00:00

abstract：:Previous work has established that activation of Mos, Mek, and p42 mitogen-activated protein (MAP) kinase can trigger release from G2-phase arrest in Xenopus oocytes and oocyte extracts and can cause Xenopus embryos and extracts to arrest in mitosis. Herein we have found that activation of the MAP kinase cascade can a...

journal_title：Molecular biology of the cell

authors： Walter SA,Guadagno TM,Ferrell JE Jr

更新日期：1997-11-01 00:00:00

abstract：:Cadherin-catenin interactions play an important role in cadherin-mediated adhesion. Here we present strong evidence that in the cadherin-catenin complex α-catenin contributes to the binding strength of another catenin, p120, to the same complex. Specifically, we found that a β-catenin-uncoupled cadherin mutant interac...

journal_title：Molecular biology of the cell

authors： Troyanovsky RB,Klingelhöfer J,Troyanovsky SM

更新日期：2011-11-01 00:00:00

abstract：:The Forkhead family of transcription factors participates in the induction of death-related genes. In NMuMG and 4T1 mammary epithelial cells, transforming growth factor beta (TGF beta) induced phosphorylation and cytoplasmic retention of the Forkhead factor FKHRL1, while reducing FHKRL1-dependent transcriptional activ...

journal_title：Molecular biology of the cell

authors： Shin I,Bakin AV,Rodeck U,Brunet A,Arteaga CL

更新日期：2001-11-01 00:00:00

abstract：:Topogenic determinants that direct protein topology at the endoplasmic reticulum membrane usually function with high fidelity to establish a uniform topological orientation for any given polypeptide. Here we show, however, that through the coupling of sequential translocation events, native topogenic determinants are ...

journal_title：Molecular biology of the cell

authors： Moss K,Helm A,Lu Y,Bragin A,Skach WR

更新日期：1998-09-01 00:00:00

abstract：:Proteolytic processing of the amyloid-β precursor protein (APP) and generation of amyloid-β peptide (Aβ) are key events in Alzheimer's disease (AD) pathogenesis. Cell biological and genetic evidence has implicated the low-density lipoprotein and sorting receptor LR11/SorLA in AD through mechanisms related to APP and A...

journal_title：Molecular biology of the cell

authors： Herskowitz JH,Offe K,Deshpande A,Kahn RA,Levey AI,Lah JJ

更新日期：2012-07-01 00:00:00

abstract：:Regulated exocytosis is thought to occur either by "full fusion," where the secretory vesicle fuses with the plasma membrane (PM) via a fusion pore that then dilates until the secretory vesicle collapses into the PM; or by "kiss-and-run," where the fusion pore does not dilate and instead rapidly reseals such that the ...

journal_title：Molecular biology of the cell

authors： Sokac AM,Bement WM

更新日期：2006-04-01 00:00:00

abstract：:We observed the dynamic recruitment of spindle checkpoint proteins Mad1 and Bub1 to detached kinetochores in budding yeast using real-time live-cell imaging and quantified recruitment in fixed cells. After induced de novo kinetochore assembly at one pair of sister centromeres, Mad1 appeared after the kinetochore prote...

journal_title：Molecular biology of the cell

authors： Krefman NI,Drubin DG,Barnes G

更新日期：2015-07-15 00:00:00

abstract：:Astrocytes in neuron-free cultures typically lack processes, although they are highly process-bearing in vivo. We show that basic fibroblast growth factor (bFGF) induces cultured astrocytes to grow processes and that Ras family GTPases mediate these morphological changes. Activated alleles of rac1 and rhoA blocked and...

journal_title：Molecular biology of the cell

authors： Kalman D,Gomperts SN,Hardy S,Kitamura M,Bishop JM

更新日期：1999-05-01 00:00:00

abstract：:Early endosomes in PC12 cells are an important site for the formation of synaptic-like microvesicles and constitutive recycling vesicles. By immunogold electron microscopy, the small GTPase rab4 was localized to early endosomes and numerous small vesicles in the cell periphery and Golgi area of PC12 cells. Overexpress...

journal_title：Molecular biology of the cell

authors： de Wit H,Lichtenstein Y,Kelly RB,Geuze HJ,Klumperman J,van der Sluijs P

更新日期：2001-11-01 00:00:00

abstract：:The Rab GTPase Ypt11 is a Myo2-binding protein implicated in mother-to-bud transport of the cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during yeast division. However, its reported subcellular localization does not reflect all of these functions. Here we show that Ypt11 is normally a low-abundanc...

journal_title：Molecular biology of the cell

authors： Lewandowska A,Macfarlane J,Shaw JM

更新日期：2013-04-01 00:00:00

abstract：:In Saccharomyces cerevisiae, many osmotically inducible genes are regulated by the Sko1p-Ssn6p-Tup1p complex. On osmotic shock, the MAP kinase Hog1p associates with this complex, phosphorylates Sko1p, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. We have found that phospholip...

journal_title：Molecular biology of the cell

authors： Guha N,Desai P,Vancura A

更新日期：2007-07-01 00:00:00