Abstract:
:Astrocytes in neuron-free cultures typically lack processes, although they are highly process-bearing in vivo. We show that basic fibroblast growth factor (bFGF) induces cultured astrocytes to grow processes and that Ras family GTPases mediate these morphological changes. Activated alleles of rac1 and rhoA blocked and reversed bFGF effects when introduced into astrocytes in dissociated culture and in brain slices using recombinant adenoviruses. By contrast, dominant negative (DN) alleles of both GTPases mimicked bFGF effects. A DN allele of Ha-ras blocked bFGF effects but not those of Rac1-DN or RhoA-DN. Our results show that bFGF acting through c-Ha-Ras inhibits endogenous Rac1 and RhoA GTPases thereby triggering astrocyte process growth, and they provide evidence for the regulation of this cascade in vivo by a yet undetermined neuron-derived factor.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Kalman D,Gomperts SN,Hardy S,Kitamura M,Bishop JMdoi
10.1091/mbc.10.5.1665keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
1665-83issue
5eissn
1059-1524issn
1939-4586journal_volume
10pub_type
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