Centrosomes split in the presence of impaired DNA integrity during mitosis.

abstract：:With the use of the patch clamp technique, the role of cytoskeleton in the regulation of ion channels in plasma membrane of leukemic K562 cells was examined. Single-channel measurements have indicated that disruption of actin filaments with cytochalasin D (CD) resulted in a considerable increase of the activity of non...

journal_title：Molecular biology of the cell

authors： Negulyaev YA,Vedernikova EA,Maximov AV

更新日期：1996-12-01 00:00:00

abstract：:GRIM-19 was found to copurify with complex I of mitochondrial respiratory chain and subsequently was demonstrated to be involved in complex I assembly and activity. To further understand its function in complex I, we dissected its functional domains by generating a number of deletion, truncation, and point mutants. Th...

journal_title：Molecular biology of the cell

authors： Lu H,Cao X

更新日期：2008-05-01 00:00:00

abstract：:Cell migration is a highly conserved process involving cytoskeletal reorganization and restructuring of the surrounding extracellular matrix. Although there are many studies describing mechanisms underlying cell motility, little has been reported about the contribution of alternative isoform use toward cell migration....

journal_title：Molecular biology of the cell

authors： Lee HN,Mitra M,Bosompra O,Corney DC,Johnson EL,Rashed N,Ho LD,Coller HA

更新日期：2018-08-01 00:00:00

abstract：:The formation and maintenance of the bipolar mitotic spindle apparatus require a complex and balanced interplay of several mechanisms, including the stabilization and separation of polar microtubules and the action of various microtubule motors. Nonmicrotubule elements are also present throughout the spindle apparatus...

journal_title：Molecular biology of the cell

authors： Yang CH,Snyder M

更新日期：1992-11-01 00:00:00

abstract：:Investigation of Caenorhabditis elegans act-5 gene function revealed that intestinal microvillus formation requires a specific actin isoform. ACT-5 is the most diverged of the five C. elegans actins, sharing only 93% identity with the other four. Green fluorescent protein reporter and immunofluorescence analysis indic...

journal_title：Molecular biology of the cell

authors： MacQueen AJ,Baggett JJ,Perumov N,Bauer RA,Januszewski T,Schriefer L,Waddle JA

更新日期：2005-07-01 00:00:00

abstract：:The potential to migrate is one of the most fundamental functions for various epithelial, mesenchymal, and immune cells. Image analysis of motile cell populations, both primary and cultured, typically reveals an intercellular variability in migration speeds. However, cell migration chromatography, the sorting of large...

journal_title：Molecular biology of the cell

authors： Arora A,Niño JLG,Myaing MZ,Chia S,Arasi B,Ravasio A,Huang RY,Dasgupta R,Biro M,Viasnoff V

更新日期：2020-12-01 00:00:00

abstract：:Previously published reports support the concept that, besides promoting homotypic intercellular adhesion, cadherins may transfer intracellular signals. However, the signaling pathways triggered by cadherin clustering and their biological significance are still poorly understood. We report herein that transfection of ...

journal_title：Molecular biology of the cell

authors： Lampugnani MG,Zanetti A,Breviario F,Balconi G,Orsenigo F,Corada M,Spagnuolo R,Betson M,Braga V,Dejana E

更新日期：2002-04-01 00:00:00

abstract：:Proper functioning of the protein-folding quality control network depends on the network's ability to discern diverse structural perturbations to the native states of its protein substrates. Despite the centrality of the detection of misfolded states to cell home-ostasis, very little is known about the exact sequence ...

journal_title：Molecular biology of the cell

authors： Furth N,Gertman O,Shiber A,Alfassy OS,Cohen I,Rosenberg MM,Doron NK,Friedler A,Ravid T

更新日期：2011-12-01 00:00:00

abstract：:An in vitro transport assay, established with a modified Shiga toxin B subunit (STxB) as a marker, has proved to be useful for the study of transport from the early/recycling endosome (EE/RE) to the trans-Golgi network (TGN). Here, we modified this assay to test antibodies to all known soluble N-ethylmaleimide-sensiti...

journal_title：Molecular biology of the cell

authors： Tai G,Lu L,Wang TL,Tang BL,Goud B,Johannes L,Hong W

更新日期：2004-09-01 00:00:00

abstract：:Previous research has found that a gamma-tubulin mutation in Schizosaccharomyces pombe is synthetically lethal with a deletion of the C-terminal motor domain kinesin-like protein gene pkl1, but the lethality of the double mutant prevents a phenotypic analysis of the synthetic interaction. We have investigated interact...

journal_title：Molecular biology of the cell

authors： Prigozhina NL,Walker RA,Oakley CE,Oakley BR

更新日期：2001-10-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is caused by homozygous mutations in human SMN1 Expression of a duplicate gene (SMN2) primarily results in skipping of exon 7 and production of an unstable protein isoform, SMNΔ7. Although SMN2 exon skipping is the principal contributor to SMA severity, mechanisms governing stability of s...

journal_title：Molecular biology of the cell

authors： Gray KM,Kaifer KA,Baillat D,Wen Y,Bonacci TR,Ebert AD,Raimer AC,Spring AM,Have ST,Glascock JJ,Gupta K,Van Duyne GD,Emanuele MJ,Lamond AI,Wagner EJ,Lorson CL,Matera AG

更新日期：2018-01-15 00:00:00

abstract：:In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key rol...

journal_title：Molecular biology of the cell

authors： Kumar S,Briguglio JS,Turkewitz AP

更新日期：2014-08-15 00:00:00

abstract：:Lumen establishment and maintenance are fundamental for tubular organs physiological functions. Most of the studies investigating the mechanisms regulating this process have been carried out in cell cultures or in smaller organisms, whereas little has been done in mammalian model systems in vivo. Here we used the sali...

journal_title：Molecular biology of the cell

authors： Shitara A,Malec L,Ebrahim S,Chen D,Bleck C,Hoffman MP,Weigert R

更新日期：2019-02-01 00:00:00

abstract：:GM3 synthase (SAT-I) is the primary glycosyltransferase responsible for the biosynthesis of ganglio-series gangliosides. In this study, we identify three isoforms of mouse SAT-I proteins, named M1-SAT-I, M2-SAT-I, and M3-SAT-I, which possess distinct lengths in their NH(2)-terminal cytoplasmic tails. These isoforms ar...

journal_title：Molecular biology of the cell

authors： Uemura S,Yoshida S,Shishido F,Inokuchi J

更新日期：2009-07-01 00:00:00

abstract：:Disruption of fast axonal transport (FAT) is an early pathological event in Alzheimer's disease (AD). Soluble amyloid-β oligomers (AβOs), increasingly recognized as proximal neurotoxins in AD, impair organelle transport in cultured neurons and transgenic mouse models. AβOs also stimulate hyperphosphorylation of the ax...

journal_title：Molecular biology of the cell

authors： Ramser EM,Gan KJ,Decker H,Fan EY,Suzuki MM,Ferreira ST,Silverman MA

更新日期：2013-08-01 00:00:00

abstract：:Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using po...

journal_title：Molecular biology of the cell

authors： Dutcher SK,Morrissette NS,Preble AM,Rackley C,Stanga J

更新日期：2002-11-01 00:00:00

abstract：:MicroRNAs (miRNAs), the tiny regulatory RNAs, form complexes with Argonaute (Ago) proteins and inhibit gene expression in metazoan cells. While studying parasite-invaded macrophages, we identify a unique mode of gene regulation in which the parasite Leishmania donovani (Ld) causes mitochondrial depolarization, reduces...

journal_title：Molecular biology of the cell

authors： Chakrabarty Y,Bhattacharyya SN

更新日期：2017-07-15 00:00:00

abstract：:The mitotic spindle is a microtubule (MT)-based molecular machine that serves for equal segregation of chromosomes during cell division. The formation of the mitotic spindle requires the activity of MT motors, including members of the kinesin-14 family. Although evidence suggests that kinesins-14 act by driving the sl...

journal_title：Molecular biology of the cell

authors： Oladipo A,Cowan A,Rodionov V

更新日期：2007-09-01 00:00:00

abstract：:Wee1 inactivates the Cdc2-cyclin B complex during interphase by phosphorylating Cdc2 on Tyr-15. The activity of Wee1 is highly regulated during the cell cycle. In frog egg extracts, it has been established previously that Xenopus Wee1 (Xwee1) is present in a hypophosphorylated, active form during interphase and underg...

journal_title：Molecular biology of the cell

authors： Lee J,Kumagai A,Dunphy WG

更新日期：2001-03-01 00:00:00

abstract：:A heterozygous Caveolin-1 c.474delA mutation has been identified in a family with heritable pulmonary arterial hypertension (PAH). This frameshift mutation leads to caveolin-1 protein that contains all known functional domains but has a change only in the final 20 amino acids of the C terminus. Here we studied how thi...

journal_title：Molecular biology of the cell

authors： Marsboom G,Chen Z,Yuan Y,Zhang Y,Tiruppathi C,Loyd JE,Austin ED,Machado RF,Minshall RD,Rehman J,Malik AB

更新日期：2017-03-15 00:00:00

abstract：:Biochemical data have shown that COPI-coated vesicles are tethered to Golgi membranes by a complex of at least three proteins: p115, giantin, and GM130. p115 binds to giantin on the vesicles and to GM130 on the membrane. We now examine the function of this tethering complex in vivo. Microinjection of an N-terminal pep...

journal_title：Molecular biology of the cell

authors： Seemann J,Jokitalo EJ,Warren G

更新日期：2000-02-01 00:00:00

abstract：:Mechanically stressed cells display increased levels of fos message and protein. Although the intracellular signaling pathways responsible for FOS induction have been extensively characterized, we still do not understand the nature of the primary cell mechanotransduction event responsible for converting an externally ...

journal_title：Molecular biology of the cell

authors： Grembowicz KP,Sprague D,McNeil PL

更新日期：1999-04-01 00:00:00

abstract：:Nucleosome positioning is important for the structural integrity of chromosomes. During metaphase the mitotic spindle exerts physical force on pericentromeric chromatin. The cell must adjust the pericentromeric chromatin to accommodate the changing tension resulting from microtubule dynamics to maintain a stable metap...

journal_title：Molecular biology of the cell

authors： Verdaasdonk JS,Gardner R,Stephens AD,Yeh E,Bloom K

更新日期：2012-07-01 00:00:00

abstract：:Alternative pre-mRNA splicing patterns can change an extracellular stimulus, but the signaling pathways leading to these changes are still poorly characterized. Here, we describe a tyrosine-phosphorylated nuclear protein, YT521-B, and show that it interacts with the nuclear transcriptosomal component scaffold attachme...

journal_title：Molecular biology of the cell

authors： Hartmann AM,Nayler O,Schwaiger FW,Obermeier A,Stamm S

更新日期：1999-11-01 00:00:00

abstract：:The mitochondrial cytochrome c oxidase assembles in the inner membrane from subunits of dual genetic origin. The assembly process of the enzyme is initiated by membrane insertion of the mitochondria-encoded Cox1 subunit. During complex maturation, transient assembly intermediates, consisting of structural subunits and...

journal_title：Molecular biology of the cell

authors： Bareth B,Nikolov M,Lorenzi I,Hildenbeutel M,Mick DU,Helbig C,Urlaub H,Ott M,Rehling P,Dennerlein S

更新日期：2016-05-15 00:00:00

abstract：:The PDZ domain-containing protein CAL mediates lysosomal trafficking and degradation of CFTR. Here we demonstrate the involvement of a CAL-binding SNARE protein syntaxin 6 (STX6) in this process. Overexpression of STX6, which colocalizes and coimmunoprecipitates with CAL, dramatically reduces the steady-state level an...

journal_title：Molecular biology of the cell

authors： Cheng J,Cebotaru V,Cebotaru L,Guggino WB

更新日期：2010-04-01 00:00:00

abstract：:DSL1 was identified through its genetic interaction with SLY1, which encodes a t-SNARE-interacting protein that functions in endoplasmic reticulum (ER)-to-Golgi traffic. Conditional dsl1 mutants exhibit a block in ER-to-Golgi traffic at the restrictive temperature. Here, we show that dsl1 mutants are defective for ret...

journal_title：Molecular biology of the cell

authors： Reilly BA,Kraynack BA,VanRheenen SM,Waters MG

更新日期：2001-12-01 00:00:00

abstract：:The polysomal ribonuclease 1 (PMR1) mRNA endonuclease forms a selective complex with its translating substrate mRNAs where it is activated to initiate mRNA decay. Previous work showed tyrosine phosphorylation is required for PMR1 targeting to this polysome-bound complex, and it identified c-Src as the responsible kina...

journal_title：Molecular biology of the cell

authors： Peng Y,Liu X,Schoenberg DR

更新日期：2008-02-01 00:00:00

abstract：:During the cell cycle, Cdc2-cyclin B kinase abruptly becomes active and triggers the entry into mitosis/meiosis. Recently, it was found that inactive Cdc2-cyclin B is present in aggregates in immature starfish oocytes and becomes disaggregated at the time of its activation during maturation. We discuss a possible scen...

journal_title：Molecular biology of the cell

authors： Slepchenko BM,Terasaki M

更新日期：2003-11-01 00:00:00

abstract：:Cell cycle-dependent morphogenesis of unicellular organisms depends on the spatiotemporal control of cell polarity. Rho GTPases are the major players that guide cells through structural reorganizations such as cytokinesis (Rho1 dependent) and polarity establishment (Cdc42 dependent). In budding yeast, the protein Gps1...

journal_title：Molecular biology of the cell

authors： Meitinger F,Pereira G

更新日期：2017-04-01 00:00:00