Abstract:
:Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using positional cloning, we have walked 720 kb and identified a 13.2-kb fragment that contains epsilon-tubulin and rescues the Bld2 defects. The bld2-1 allele has a premature stop codon and intragenic revertants replace the stop codon with glutamine, glutamate, or lysine. Polyclonal antibodies to epsilon-tubulin show peripheral labeling of full-length basal bodies and centrioles. Thus, epsilon-tubulin is encoded by the BLD2 allele and epsilon-tubulin plays a role in basal body/centriole morphogenesis.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Dutcher SK,Morrissette NS,Preble AM,Rackley C,Stanga Jdoi
10.1091/mbc.e02-04-0205keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
3859-69issue
11eissn
1059-1524issn
1939-4586journal_volume
13pub_type
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