Regulated phosphorylation of budding yeast's essential myosin V heavy chain, Myo2p.

abstract：:In mammals, a finite population of oocytes is generated during embryogenesis, and proper oocyte meiotic divisions are crucial for fertility. Sperm-associated antigen 1 (SPAG-1) has been implicated in infertility and tumorigenesis; however, its relevance in cell cycle programs remains rudimentary. Here we explore a nov...

journal_title：Molecular biology of the cell

authors： Huang C,Wu D,Khan FA,Jiao X,Guan K,Huo L

更新日期：2016-06-01 00:00:00

abstract：:Microtubules (MTs) often form a polarized array with minus-ends anchored at the centrosome and plus-ends extended towards the cell margins. Plus-ends display behavior known as dynamic instability, - transitions between rapid shortening and slow growth. It is known that dynamic instability is regulated locally to ensur...

journal_title：Molecular biology of the cell

authors： Burakov A,Vorobjev I,Semenova I,Cowan A,Carson J,Wu Y,Rodionov V

更新日期：2021-01-13 00:00:00

abstract：:Werner syndrome (WS) is a human progeroid syndrome characterized by the early onset of a large number of clinical features associated with the normal aging process. The complex molecular and cellular phenotypes of WS involve characteristic features of genomic instability and accelerated replicative senescence. The gen...

journal_title：Molecular biology of the cell

authors： Balajee AS,Machwe A,May A,Gray MD,Oshima J,Martin GM,Nehlin JO,Brosh R,Orren DK,Bohr VA

更新日期：1999-08-01 00:00:00

abstract：:We identify a new mechanism for the beta(1)-adrenergic receptor (beta(1)AR)-mediated regulation of human ether-a-go-go-related gene (HERG) potassium channel (Kv11.1). We find that the previously reported modulatory interaction between Kv11.1 channels and 14-3-3epsilon proteins is competed by wild type beta(1)AR by mea...

journal_title：Molecular biology of the cell

authors： Tutor AS,Delpón E,Caballero R,Gómez R,Núñez L,Vaquero M,Tamargo J,Mayor F Jr,Penela P

更新日期：2006-11-01 00:00:00

abstract：:Loss-of-function mutations in the nonconventional myosin Vb (Myo5b) result in microvillus inclusion disease (MVID) and massive secretory diarrhea that often begins at birth. Myo5b mutations disrupt the apical recycling endosome (ARE) and membrane traffic, resulting in reduced surface expression of apical membrane prot...

journal_title：Molecular biology of the cell

authors： Forteza R,Ahsan MK,Cartón-García F,Arango D,Ameen NA,Salas PJ

更新日期：2019-12-15 00:00:00

abstract：:The membrane-anchored atlastin GTPase couples nucleotide hydrolysis to the catalysis of homotypic membrane fusion to form a branched endoplasmic reticulum network. Trans dimerization between atlastins anchored in opposing membranes, accompanied by a cross-over conformational change, is thought to draw the membranes to...

journal_title：Molecular biology of the cell

authors： Saini SG,Liu C,Zhang P,Lee TH

更新日期：2014-12-01 00:00:00

abstract：:m-AAA proteases are ATP-dependent proteolytic machines in the inner membrane of mitochondria which are crucial for the maintenance of mitochondrial activities. Conserved nuclear-encoded subunits, termed paraplegin, Afg3l1, and Afg3l2, form various isoenzymes differing in their subunit composition in mammalian mitochon...

journal_title：Molecular biology of the cell

authors： Koppen M,Bonn F,Ehses S,Langer T

更新日期：2009-10-01 00:00:00

abstract：:During mating, Saccharomyces cerevisiae cells must degrade the intervening cell wall to allow fusion of the partners. Because improper timing or location of cell wall degradation would cause lysis, the initiation of cell fusion must be highly regulated. Here, we find that yeast cell fusion is negatively regulated by c...

journal_title：Molecular biology of the cell

authors： Hall AE,Rose MD

更新日期：2019-02-15 00:00:00

abstract：:We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the horm...

journal_title：Molecular biology of the cell

authors： McIsaac RS,Silverman SJ,McClean MN,Gibney PA,Macinskas J,Hickman MJ,Petti AA,Botstein D

更新日期：2011-11-01 00:00:00

abstract：:Transporting epithelial cells optimize their morphology for solute uptake by building an apical specialization: a dense array of microvilli that serves to increase membrane surface area. In the intestinal tract, individual cells build thousands of microvilli, which pack tightly to form the brush border. Recent studies...

journal_title：Molecular biology of the cell

authors： Pinette JA,Mao S,Millis BA,Krystofiak ES,Faust JJ,Tyska MJ

更新日期：2019-01-01 00:00:00

abstract：:Numb has been implicated in cortical neurogenesis during nervous system development, as a result of its asymmetric partitioning and antagonizing Notch signaling. Recent studies have revealed that Numb functions in clathrin-dependent endocytosis by binding to the AP-2 complex. Numb is also expressed in postmitotic neur...

journal_title：Molecular biology of the cell

authors： Nishimura T,Yamaguchi T,Tokunaga A,Hara A,Hamaguchi T,Kato K,Iwamatsu A,Okano H,Kaibuchi K

更新日期：2006-03-01 00:00:00

abstract：:Phospholipase D (PLD) has been suggested to mediate epidermal growth factor (EGF) signaling. However, the molecular mechanism of EGF-induced PLD activation has not yet been elucidated. We investigated the importance of the phosphorylation and compartmentalization of PLD1 in EGF signaling. EGF treatment of COS-7 cells ...

journal_title：Molecular biology of the cell

authors： Han JM,Kim Y,Lee JS,Lee CS,Lee BD,Ohba M,Kuroki T,Suh PG,Ryu SH

更新日期：2002-11-01 00:00:00

abstract：:Two different human diseases, X-linked myotubular myopathy and Charcot-Marie-Tooth disease, result from mutant MTM1 or MTMR2 lipid phosphatases. Although events involved in endosomal PI(3)P and PI(3,5)P(2) synthesis are well established and pivotal in receptor signaling and degradation, enzymes involved in phosphoinos...

journal_title：Molecular biology of the cell

authors： Cao C,Backer JM,Laporte J,Bedrick EJ,Wandinger-Ness A

更新日期：2008-08-01 00:00:00

abstract：:Intracellular free calcium ([Ca2+]i) has been proposed to play an important part in the regulation of the cell cycle. Although a number of studies have shown that stimulation of quiescent cells with growth factors causes an immediate rise in [Ca2+]i (Rabinovitch et al., 1986; Vincentini and Villereal, 1986; Hesketh et...

journal_title：Molecular biology of the cell

authors： Wahl M,Gruenstein E

更新日期：1993-03-01 00:00:00

abstract：:We identified a direct interaction between the neuronal transmembrane protein calsyntenin-1 and the light chain of Kinesin-1 (KLC1). GST pulldowns demonstrated that two highly conserved segments in the cytoplasmic domain of calsyntenin-1 mediate binding to the tetratricopeptide repeats of KLC1. A complex containing ca...

journal_title：Molecular biology of the cell

authors： Konecna A,Frischknecht R,Kinter J,Ludwig A,Steuble M,Meskenaite V,Indermühle M,Engel M,Cen C,Mateos JM,Streit P,Sonderegger P

更新日期：2006-08-01 00:00:00

abstract：:Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are...

journal_title：Molecular biology of the cell

authors： Gutiérrez-Escribano P,González-Novo A,Suárez MB,Li CR,Wang Y,de Aldana CR,Correa-Bordes J

更新日期：2011-07-15 00:00:00

abstract：:Disassembly of the epithelial apical junctional complex (AJC), composed of the tight junction (TJ) and adherens junction (AJ), is important for normal tissue remodeling and pathogen-induced disruption of epithelial barriers. Using a calcium depletion model in T84 epithelial cells, we previously found that disassembly ...

journal_title：Molecular biology of the cell

authors： Ivanov AI,McCall IC,Parkos CA,Nusrat A

更新日期：2004-06-01 00:00:00

abstract：:Regulator of G protein signaling (RGS) proteins inhibit G protein signaling by activating Galpha GTPase activity, but the mechanisms that regulate RGS activity are not well understood. The mammalian R7 binding protein (R7BP) can interact with all members of the R7 family of RGS proteins, and palmitoylation of R7BP can...

journal_title：Molecular biology of the cell

authors： Porter MY,Koelle MR

更新日期：2010-01-15 00:00:00

abstract：:In Schizosaccharomyces pombe, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring (CR). A single essential formin, Cdc12, localizes to the cell middle upon mitotic onset and nucleates the F-actin of the CR. Cdc12 medial recruitment is mediated in part by its direct binding to the...

journal_title：Molecular biology of the cell

authors： Willet AH,Bohnert KA,Gould KL

更新日期：2018-03-15 00:00:00

abstract：:The assembly of four soluble N-ethylmaleimide-sensitive factor attachment protein receptor domains into a complex is essential for membrane fusion. In most cases, the four SNARE-domains are encoded by separate membrane-targeted proteins. However, in the exocytotic pathway, two SNARE-domains are present in one protein,...

journal_title：Molecular biology of the cell

authors： Nagy G,Milosevic I,Mohrmann R,Wiederhold K,Walter AM,Sørensen JB

更新日期：2008-09-01 00:00:00

abstract：:Fusion of yeast vacuoles requires the Rab GTPase Ypt7p, four SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), the SNARE disassembly chaperones Sec17p/Sec18p, vacuolar lipids, and the Rab-effector complex HOPS (homotypic fusion and vacuole protein sorting). Two HOPS subunits have direct ...

journal_title：Molecular biology of the cell

authors： Orr A,Wickner W,Rusin SF,Kettenbach AN,Zick M

更新日期：2015-01-15 00:00:00

abstract：:To investigate the molecular interactions of synaptophysin I and vesicle-associated membrane protein 2 (VAMP2)/synaptobrevin II during exocytosis, we have used time-lapse videomicroscopy to measure fluorescence resonance energy transfer in live neurons. For this purpose, fluorescent protein variants fused to synaptoph...

journal_title：Molecular biology of the cell

authors： Pennuto M,Dunlap D,Contestabile A,Benfenati F,Valtorta F

更新日期：2002-08-01 00:00:00

abstract：:When Pichia pastoris adapts from methanol to glucose growth, peroxisomes are rapidly sequestered and degraded within the vacuole by micropexophagy. During micropexophagy, sequestering membranes arise from the vacuole to engulf the peroxisomes. Fusion of the sequestering membranes and incorporation of the peroxisomes i...

journal_title：Molecular biology of the cell

authors： Chang T,Schroder LA,Thomson JM,Klocman AS,Tomasini AJ,Strømhaug PE,Dunn WA Jr

更新日期：2005-10-01 00:00:00

abstract：:All vertebrates contain two nonmuscle myosin II heavy chains, A and B, which differ in tissue expression and subcellular distributions. To understand how these distinct distributions are controlled and what role they play in cell migration, myosin IIA and IIB were examined during wound healing by bovine aortic endothe...

journal_title：Molecular biology of the cell

authors： Kolega J

更新日期：2003-12-01 00:00:00

abstract：:Compositional analyses of nucleic acids and proteins have shed light on possible origins of living cells. In this work, rigorous compositional analyses of ∼5000 plasma membrane lipid constituents of 273 species in the three life domains (archaea, eubacteria, and eukaryotes) revealed a remarkable statistical paradox, i...

journal_title：Molecular biology of the cell

authors： Bansal S,Mittal A

更新日期：2015-04-01 00:00:00

abstract：:The insulin-stimulated trafficking of GLUT4 to the plasma membrane in muscle and fat tissue constitutes a central process in blood glucose homeostasis. The tethering, docking, and fusion of GLUT4 vesicles with the plasma membrane (PM) represent the most distal steps in this pathway and have been recently shown to be k...

journal_title：Molecular biology of the cell

authors： Lopez JA,Burchfield JG,Blair DH,Mele K,Ng Y,Vallotton P,James DE,Hughes WE

更新日期：2009-09-01 00:00:00

abstract：:Mutations in the cytolinker protein plectin lead to grossly distorted morphology of neuromuscular junctions (NMJs) in patients suffering from epidermolysis bullosa simplex (EBS)-muscular dystrophy (MS) with myasthenic syndrome (MyS). Here we investigated whether plectin contributes to the structural integrity of NMJs ...

journal_title：Molecular biology of the cell

authors： Mihailovska E,Raith M,Valencia RG,Fischer I,Al Banchaabouchi M,Herbst R,Wiche G

更新日期：2014-12-15 00:00:00

abstract：:The polysomal ribonuclease 1 (PMR1) mRNA endonuclease forms a selective complex with its translating substrate mRNAs where it is activated to initiate mRNA decay. Previous work showed tyrosine phosphorylation is required for PMR1 targeting to this polysome-bound complex, and it identified c-Src as the responsible kina...

journal_title：Molecular biology of the cell

authors： Peng Y,Liu X,Schoenberg DR

更新日期：2008-02-01 00:00:00

abstract：:Type I interferons (IFN-alpha/beta) induce apoptosis in certain tumor cell lines but not others. Here we describe a mutation in STAT2 that confers an apoptotic effect in tumor cells in response to type I IFNs. This mutation was introduced in a conserved motif, PYTK, located in the STAT SH2 domain, which is shared by S...

journal_title：Molecular biology of the cell

authors： Scarzello AJ,Romero-Weaver AL,Maher SG,Veenstra TD,Zhou M,Qin A,Donnelly RP,Sheikh F,Gamero AM

更新日期：2007-07-01 00:00:00

abstract：:The expression of FREQUENCY, a central component of the circadian clock in Neurospora crassa, shows daily cycles that are exquisitely sensitive to the environment. Two forms of FRQ that differ in length by 99 amino acids, LFRQ and SFRQ, are synthesized from alternative initiation codons and the change in their ratio a...

journal_title：Molecular biology of the cell

authors： Colot HV,Loros JJ,Dunlap JC

更新日期：2005-12-01 00:00:00