Abstract:
:Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are regulated. In Candida albicans, the cyclin-dependent kinase (CDK) Cdc28 and the NDR kinase Cbk1 are required for hyphal growth. Here we demonstrate that Mob2, the Cbk1 activator, undergoes a Cdc28-dependent differential phosphorylation on hyphal induction. Mutations in the four CDK consensus sites in Mob2 to Ala significantly impaired hyphal development. The mutant cells produced short hyphae with enlarged tips that displayed an illicit activation of cell separation. We also show that Cdc28 phosphorylation of Mob2 is essential for the maintenance of polarisome components at hyphal tips but not at bud tips during yeast growth. Thus we have found a novel signaling pathway by which Cdc28 controls Cbk1 through the regulatory phosphorylation of Mob2, which is crucial for normal hyphal development.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Gutiérrez-Escribano P,González-Novo A,Suárez MB,Li CR,Wang Y,de Aldana CR,Correa-Bordes Jdoi
10.1091/mbc.E11-03-0205subject
Has Abstractpub_date
2011-07-15 00:00:00pages
2458-69issue
14eissn
1059-1524issn
1939-4586pii
mbc.E11-03-0205journal_volume
22pub_type
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