Abstract:
:Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in cytosolic LD dynamics. We have found that TGH deficiency resulted in decreased size and increased number of LDs in hepatocytes. Using fluorescent fatty acid analogues to trace LD formation, we observed that TGH deficiency did not affect the formation of nascent LDs on the ER. However, the rate of lipid transfer into preformed LDs was significantly slower in the absence of TGH. Absence of TGH expression resulted in increased levels of membrane diacylglycerol and augmented phospholipid synthesis, which may be responsible for the delayed lipid transfer. Therefore, altered maturation (growth) rather than nascent formation (de novo synthesis) may be responsible for the observed morphological changes of LDs in TGH-deficient hepatocytes.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Wang H,Wei E,Quiroga AD,Sun X,Touret N,Lehner Rdoi
10.1091/mbc.e09-05-0364subject
Has Abstractpub_date
2010-06-15 00:00:00pages
1991-2000issue
12eissn
1059-1524issn
1939-4586pii
E09-05-0364journal_volume
21pub_type
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