The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation.

abstract：:The kidney, together with bone and intestine, plays a crucial role in maintaining whole-body calcium (Ca(2+)) homoeostasis, which is primarily mediated by altering the reabsorption of Ca(2+) filtered by the glomerulus. The transient receptor potential-vanilloid-5 (TRPV5) channel protein forms a six- transmembrane Ca(2...

journal_title：Molecular biology of the cell

authors： Cai X,Srivastava S,Surindran S,Li Z,Skolnik EY

更新日期：2014-04-01 00:00:00

abstract：:The zona pellucida (ZP) is a highly organized extracellular coat that surrounds all mammalian eggs. The mouse egg ZP is composed of three glycoproteins, called mZP1-3, that are synthesized, secreted, and assembled into a ZP exclusively by growing oocytes. Here, we microinjected epitope-tagged (Myc and Flag) cDNAs for ...

journal_title：Molecular biology of the cell

authors： Qi H,Williams Z,Wassarman PM

更新日期：2002-02-01 00:00:00

abstract：:In this study, we examined the subcellular distribution and functions of LIMK1 in developing neurons. Confocal microscopy, subcellular fractionation, and expression of several epitope-tagged LIMK1 constructs revealed that LIMK1 is enriched in the Golgi apparatus and growth cones, with the LIM domain required for Golgi...

journal_title：Molecular biology of the cell

authors： Rosso S,Bollati F,Bisbal M,Peretti D,Sumi T,Nakamura T,Quiroga S,Ferreira A,Cáceres A

更新日期：2004-07-01 00:00:00

abstract：:We have shown previously that the transforming growth factor-beta (TGFbeta)-regulated Sma-Mad (Smad) protein 3 and Smad4 proteins transactivate the apolipoprotein C-III promoter in hepatic cells via a hormone response element that binds the nuclear receptor hepatocyte nuclear factor 4 (HNF-4). In the present study, we...

journal_title：Molecular biology of the cell

authors： Chou WC,Prokova V,Shiraishi K,Valcourt U,Moustakas A,Hadzopoulou-Cladaras M,Zannis VI,Kardassis D

更新日期：2003-03-01 00:00:00

abstract：:Focal adhesion kinase (FAK) is a cytoplasmic tyrosine kinase protein localized to regions called focal adhesions, which are contact points between cells and the extracellular matrix. FAK protein acts as a scaffold to transfer adhesion-dependent and growth factor signals into the cell. Increased FAK expression is linke...

journal_title：Molecular biology of the cell

authors： Fonar Y,Gutkovich YE,Root H,Malyarova A,Aamar E,Golubovskaya VM,Elias S,Elkouby YM,Frank D

更新日期：2011-07-01 00:00:00

abstract：:Microtubules (MTs) in newt mitotic spindles grow faster than MTs in the interphase cytoplasmic microtubule complex (CMTC), yet spindle MTs do not have the long lengths or lifetimes of the CMTC microtubules. Because MTs undergo dynamic instability, it is likely that changes in the durations of growth or shortening are ...

journal_title：Molecular biology of the cell

authors： Gliksman NR,Skibbens RV,Salmon ED

更新日期：1993-10-01 00:00:00

abstract：:Mathematical modeling has been instrumental in identifying common principles of cell polarity across diverse systems. These principles include positive feedback loops that are required to destabilize a spatially uniform state of the cell. The conserved small G-protein Cdc42 is a master regulator of eukaryotic cellular...

journal_title：Molecular biology of the cell

authors： Goryachev AB,Leda M

更新日期：2017-02-01 00:00:00

abstract：:In cycling between the mammalian host and the tsetse fly vector, trypanosomes undergo major changes in energy metabolism and surface coat composition. Early procyclic (insect) forms in the tsetse fly midgut are coated by glycoproteins known as EP and GPEET procyclins. EP expression continues in late procyclic forms, w...

journal_title：Molecular biology of the cell

authors： Vassella E,Probst M,Schneider A,Studer E,Renggli CK,Roditi I

更新日期：2004-09-01 00:00:00

abstract：:Keratins are cytoplasmic intermediate filament proteins preferentially expressed by epithelial tissues in a site-specific and differentiation-dependent manner. The complex network of keratin filaments in stratified epithelia is tightly regulated during squamous cell differentiation. Keratin 14 (K14) is expressed in mi...

journal_title：Molecular biology of the cell

authors： Alam H,Sehgal L,Kundu ST,Dalal SN,Vaidya MM

更新日期：2011-11-01 00:00:00

abstract：:Chediak-Higashi syndrome is a genetic disorder caused by mutations in a gene encoding a protein named LYST in humans ("lysosomal trafficking regulator") or Beige in mice. A prominent feature of this disease is the accumulation of enlarged lysosome-related granules in a variety of cells. The genome of Dictyostelium dis...

journal_title：Molecular biology of the cell

authors： Harris E,Wang N,Wu Wl WL,Weatherford A,De Lozanne A,Cardelli J

更新日期：2002-02-01 00:00:00

abstract：:Accurate chromosome alignment at metaphase facilitates the equal segregation of sister chromatids to each of the nascent daughter cells. Lack of proper metaphase alignment is an indicator of defective chromosome congression and aberrant kinetochore-microtubule attachments which in turn promotes chromosome missegregati...

journal_title：Molecular biology of the cell

authors： Braga LG,Prifti DK,Garand C,Saini PK,Elowe S

更新日期：2020-10-21 00:00:00

abstract：:Yeast that naturally exhaust the glucose from their environment differentiate into three distinct cell types distinguishable by flow cytometry. Among these is a quiescent (Q) population, which is so named because of its uniform but readily reversed G1 arrest, its fortified cell walls, heat tolerance, and longevity. Da...

journal_title：Molecular biology of the cell

authors： Li L,Miles S,Melville Z,Prasad A,Bradley G,Breeden LL

更新日期：2013-12-01 00:00:00

abstract：:In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key rol...

journal_title：Molecular biology of the cell

authors： Kumar S,Briguglio JS,Turkewitz AP

更新日期：2014-08-15 00:00:00

abstract：:Starvation of Dictyostelium induces a developmental program in which cells form an aggregate that eventually differentiates into a multicellular structure. The aggregate formation is mediated by directional migration of individual cells that quickly transition to group migration in which cells align in a head-to-tail ...

journal_title：Molecular biology of the cell

authors： Garcia GL,Rericha EC,Heger CD,Goldsmith PK,Parent CA

更新日期：2009-07-01 00:00:00

abstract：:Lumen establishment and maintenance are fundamental for tubular organs physiological functions. Most of the studies investigating the mechanisms regulating this process have been carried out in cell cultures or in smaller organisms, whereas little has been done in mammalian model systems in vivo. Here we used the sali...

journal_title：Molecular biology of the cell

authors： Shitara A,Malec L,Ebrahim S,Chen D,Bleck C,Hoffman MP,Weigert R

更新日期：2019-02-01 00:00:00

abstract：:The demand for phenomics, a high-dimensional and high-throughput phenotyping method, has been increasing in many fields of biology. The budding yeast Saccharomyces cerevisiae, a unicellular model organism, provides an invaluable system for dissecting complex cellular processes using high-resolution phenotyping. Moreov...

journal_title：Molecular biology of the cell

authors： Ohya Y,Kimori Y,Okada H,Ohnuki S

更新日期：2015-11-05 00:00:00

abstract：:Differentiation-inducing factor-1 (DIF-1) is a polyketide that induces Dictyostelium amoebae to differentiate as prestalk cells. We performed a global quantitative screen for phosphorylation changes that occur within the first minutes after addition of DIF-1, using a triple-label SILAC approach. This revealed a new wo...

journal_title：Molecular biology of the cell

authors： Sugden C,Urbaniak MD,Araki T,Williams JG

更新日期：2015-02-15 00:00:00

abstract：:In response to stress conditions (such as nutrient limitation or accumulation of damaged organelles) and certain pathological situations, eukaryotic cells use autophagy as a survival mechanism. During nutrient stress the main purpose of autophagy is to degrade cytoplasmic materials within the lysosome/vacuole lumen an...

journal_title：Molecular biology of the cell

authors： Yang Z,Huang J,Geng J,Nair U,Klionsky DJ

更新日期：2006-12-01 00:00:00

abstract：:The proper positioning of mitotic spindle in the single-cell Caenorhabditis elegans embryo is achieved initially by the migration and rotation of the pronuclear complex (PNC) and its two associated astral microtubules (MTs). Pronuclear migration produces global cytoplasmic flows that couple the mechanics of all MTs, t...

journal_title：Molecular biology of the cell

authors： Nazockdast E,Rahimian A,Needleman D,Shelley M

更新日期：2017-11-07 00:00:00

abstract：:Intracellular free calcium ([Ca2+]i) has been proposed to play an important part in the regulation of the cell cycle. Although a number of studies have shown that stimulation of quiescent cells with growth factors causes an immediate rise in [Ca2+]i (Rabinovitch et al., 1986; Vincentini and Villereal, 1986; Hesketh et...

journal_title：Molecular biology of the cell

authors： Wahl M,Gruenstein E

更新日期：1993-03-01 00:00:00

abstract：:The MTG1 gene of Saccharomyces cerevisiae, corresponding to ORF YMR097c on chromosome XIII, codes for a mitochondrial protein essential for respiratory competence. A human homologue of Mtg1p capable of partially rescuing the respiratory deficiency of a yeast mtg1 mutant has also been localized in mitochondria. Mtg1p i...

journal_title：Molecular biology of the cell

authors： Barrientos A,Korr D,Barwell KJ,Sjulsen C,Gajewski CD,Manfredi G,Ackerman S,Tzagoloff A

更新日期：2003-06-01 00:00:00

abstract：:The septins are a family of homologous proteins that were originally identified in Saccharomyces cerevisiae, where they are associated with the "neck filaments" and are involved in cytokinesis and other aspects of the organization of the cell surface. We report here the identification of Sep1, a Drosophila melanogaste...

journal_title：Molecular biology of the cell

authors： Fares H,Peifer M,Pringle JR

更新日期：1995-12-01 00:00:00

abstract：:Stem cells are classically defined by their multipotent, long-term proliferation, and self-renewal capabilities. Here, we show that increased antioxidant capacity represents an additional functional characteristic of muscle-derived stem cells (MDSCs). Seeking to understand the superior regenerative capacity of MDSCs c...

journal_title：Molecular biology of the cell

authors： Urish KL,Vella JB,Okada M,Deasy BM,Tobita K,Keller BB,Cao B,Piganelli JD,Huard J

更新日期：2009-01-01 00:00:00

abstract：:ELMO2 belongs to a family of scaffold proteins involved in phagocytosis and cell motility. ELMO2 can simultaneously bind integrin-linked kinase (ILK) and RhoG, forming tripartite ERI complexes. These complexes are involved in promoting β1 integrin-dependent directional migration in undifferentiated epidermal keratinoc...

journal_title：Molecular biology of the cell

authors： Jackson BC,Ivanova IA,Dagnino L

更新日期：2015-07-15 00:00:00

abstract：:Endocytic adaptor proteins facilitate cargo recruitment and clathrin-coated pit nucleation. The prototypical clathrin adaptor AP2 mediates cargo recruitment, maturation, and scission of the pit by binding cargo, clathrin, and accessory proteins, including the Eps-homology (EH) domain proteins Eps15 and intersectin. Ho...

journal_title：Molecular biology of the cell

authors： Teckchandani A,Mulkearns EE,Randolph TW,Toida N,Cooper JA

更新日期：2012-08-01 00:00:00

abstract：:We observed the dynamic recruitment of spindle checkpoint proteins Mad1 and Bub1 to detached kinetochores in budding yeast using real-time live-cell imaging and quantified recruitment in fixed cells. After induced de novo kinetochore assembly at one pair of sister centromeres, Mad1 appeared after the kinetochore prote...

journal_title：Molecular biology of the cell

authors： Krefman NI,Drubin DG,Barnes G

更新日期：2015-07-15 00:00:00

abstract：:Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises from light excitation. An alternative is luciferase, an enzyme that ...

journal_title：Molecular biology of the cell

authors： Mazo-Vargas A,Park H,Aydin M,Buchler NE

更新日期：2014-11-05 00:00:00

abstract：:Cdc25, the dual-specificity phosphatase that dephosphorylates the Cdc2-cyclin B complex at mitosis, is highly regulated during the cell cycle. In Xenopus egg extracts, Cdc25 is associated with two isoforms of the 14-3-3 protein. Cdc25 is complexed primarily with 14-3-3epsilon and to a lesser extent with 14-3-3zeta. Th...

journal_title：Molecular biology of the cell

authors： Kumagai A,Yakowec PS,Dunphy WG

更新日期：1998-02-01 00:00:00

abstract：:The development and progression of cardiac hypertrophy often leads to heart failure and death, and important modulators of hypertrophy include the histone deacetylase proteins (HDACs). Estrogen inhibits cardiac hypertrophy and progression in animal models and humans. We therefore investigated the influence of 17-β-est...

journal_title：Molecular biology of the cell

authors： Pedram A,Razandi M,Narayanan R,Dalton JT,McKinsey TA,Levin ER

更新日期：2013-12-01 00:00:00

abstract：:McKusick-Kaufman syndrome (MKKS) is a recessively inherited human genetic disease characterized by several developmental anomalies. Mutations in the MKKS gene also cause Bardet-Biedl syndrome (BBS), a genetically heterogeneous disorder with pleiotropic symptoms. However, little is known about how MKKS mutations lead t...

journal_title：Molecular biology of the cell

authors： Hirayama S,Yamazaki Y,Kitamura A,Oda Y,Morito D,Okawa K,Kimura H,Cyr DM,Kubota H,Nagata K

更新日期：2008-03-01 00:00:00