Abstract:
:Accurate chromosome alignment at metaphase facilitates the equal segregation of sister chromatids to each of the nascent daughter cells. Lack of proper metaphase alignment is an indicator of defective chromosome congression and aberrant kinetochore-microtubule attachments which in turn promotes chromosome missegregation and aneuploidy, hallmarks of cancer. Tools to sensitively, accurately and quantitatively measure chromosome alignment at metaphase will facilitate understanding of the contribution of chromosome segregation errors to the development of aneuploidy. In this work, we have developed and validated a method based on analytical geometry to measure several indicators of chromosome misalignment. We generated semi-automated and flexible ImageJ2/Fiji pipelines to quantify kinetochore misalignment at metaphase plates as well as lagging chromosomes at anaphase. These tools will ultimately allow sensitive and systematic quantitation of these chromosome segregation defects in cells undergoing mitosis.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Braga LG,Prifti DK,Garand C,Saini PK,Elowe Sdoi
10.1091/mbc.E20-09-0585subject
Has Abstractpub_date
2020-10-21 00:00:00pages
mbcE20090585eissn
1059-1524issn
1939-4586pub_type
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