Cytoplasmic flows as signatures for the mechanics of mitotic positioning.

abstract：:Formation of multiple-protein macromolecular complexes at specialized subcellular microdomains increases the specificity and efficiency of signaling in cells. In this study, we demonstrate that phosphodiesterase type 3A (PDE3A) physically and functionally interacts with cystic fibrosis transmembrane conductance regula...

journal_title：Molecular biology of the cell

authors： Penmatsa H,Zhang W,Yarlagadda S,Li C,Conoley VG,Yue J,Bahouth SW,Buddington RK,Zhang G,Nelson DJ,Sonecha MD,Manganiello V,Wine JJ,Naren AP

更新日期：2010-03-15 00:00:00

abstract：:Shrew-1 was previously isolated from an endometriotic cell line in our search for invasion-associated genes. It proved to be a membrane protein that targets to the basolateral membrane of polarized epithelial cells, interacting with E-cadherin-catenin complexes of adherens junctions. Paradoxically, the existence of ad...

journal_title：Molecular biology of the cell

authors： Schreiner A,Ruonala M,Jakob V,Suthaus J,Boles E,Wouters F,Starzinski-Powitz A

更新日期：2007-04-01 00:00:00

abstract：:The anaphase-promoting complex in partnership with its activator, Cdh1, is an E3 ubiquitin ligase responsible for targeting cell cycle proteins during G1 phase. In the budding yeast Saccharomyces cerevisiae, Cdh1 associates with the deubiquitinating enzyme Ubp15, but the significance of this interaction is unclear. To...

journal_title：Molecular biology of the cell

authors： Ostapenko D,Burton JL,Solomon MJ

更新日期：2015-06-15 00:00:00

abstract：:Stimulation of the T-cell receptor (TCR) results in the activation of several transcription factors, including NF-kappaB, that are crucial for T-cell proliferation and gain of effector functions. On TCR engagement, several proteins within the TCR-directed NF-kappaB signaling pathway undergo dynamic spatial redistribut...

journal_title：Molecular biology of the cell

authors： Rossman JS,Stoicheva NG,Langel FD,Patterson GH,Lippincott-Schwartz J,Schaefer BC

更新日期：2006-05-01 00:00:00

abstract：:Drosophila bristle cells are shaped during growth by longitudinal bundles of cross-linked actin filaments attached to the plasma membrane. We used confocal and electron microscopy to examine actin bundle structure and found that during bristle elongation, snarls of uncross-linked actin filaments and small internal bun...

journal_title：Molecular biology of the cell

authors： Tilney LG,Connelly PS,Ruggiero L,Vranich KA,Guild GM

更新日期：2003-10-01 00:00:00

abstract：:Sm-like (Lsm) proteins function in a variety of RNA-processing events. In yeast, the Lsm2-Lsm8 complex binds and stabilizes the spliceosomal U6 snRNA, whereas the Lsm1-Lsm7 complex functions in mRNA decay. Here we report that a third Lsm complex, consisting of Lsm2-Lsm7 proteins, associates with snR5, a box H/ACA snoR...

journal_title：Molecular biology of the cell

authors： Fernandez CF,Pannone BK,Chen X,Fuchs G,Wolin SL

更新日期：2004-06-01 00:00:00

abstract：:Motor neuron (MN) diseases are progressive disorders resulting from degeneration of neuromuscular junctions (NMJs), which form the connection between MNs and muscle fibers. NMJ-in-a-dish models have been developed to examine human MN-associated dysfunction with disease; however such coculture models have randomly orie...

journal_title：Molecular biology of the cell

authors： Happe CL,Tenerelli KP,Gromova AK,Kolb F,Engler AJ

更新日期：2017-07-07 00:00:00

abstract：:We conducted a genetic screen for mutations in myospheroid, the gene encoding the Drosophila betaPS integrin subunit, and identified point mutants in all of the structural domains of the protein. Surprisingly, we find that mutations in very strongly conserved residues will often allow sufficient integrin function to s...

journal_title：Molecular biology of the cell

authors： Jannuzi AL,Bunch TA,West RF,Brower DL

更新日期：2004-08-01 00:00:00

abstract：:Little is known about the mechanisms that determine localization of proteins to the plasma membrane in Saccharomyces cerevisiae. The length of the transmembrane domains and association of proteins with lipid rafts have been proposed to play a role in sorting to the cell surface. Here, we report that Fus1p, an O-glycos...

journal_title：Molecular biology of the cell

authors： Proszynski TJ,Simons K,Bagnat M

更新日期：2004-04-01 00:00:00

abstract：:Growth cone responses to guidance cues provide the basis for neuronal pathfinding. Although many cues have been identified, less is known about how signals are translated into the cytoskeletal rearrangements that steer directional changes during pathfinding. Here we show that the response of dorsal root ganglion (DRG)...

journal_title：Molecular biology of the cell

authors： Brown JA,Wysolmerski RB,Bridgman PC

更新日期：2009-02-01 00:00:00

abstract：:TPA-inducible sequence 11b/butyrate response factor 1 (TIS11b/BRF1) belongs to the tristetraprolin (TTP) family of zinc-finger proteins, which bind to mRNAs containing AU-rich elements in their 3'-untranslated region and target them for degradation. Regulation of TTP family function through phosphorylation by p38 MAP ...

journal_title：Molecular biology of the cell

authors： Rataj F,Planel S,Desroches-Castan A,Le Douce J,Lamribet K,Denis J,Feige JJ,Cherradi N

更新日期：2016-12-01 00:00:00

abstract：:In the fission yeast Schizosaccharomyces pombe, centromeres remain clustered at the spindle-pole body (SPB) during mitotic interphase. In contrast, during meiotic prophase centromeres dissociate from the SPB. Here we examined the behavior of centromere proteins in living meiotic cells of S. pombe. We show that the Nuf...

journal_title：Molecular biology of the cell

authors： Asakawa H,Hayashi A,Haraguchi T,Hiraoka Y

更新日期：2005-05-01 00:00:00

abstract：:Microtubules (MTs) often form a polarized array with minus-ends anchored at the centrosome and plus-ends extended towards the cell margins. Plus-ends display behavior known as dynamic instability, - transitions between rapid shortening and slow growth. It is known that dynamic instability is regulated locally to ensur...

journal_title：Molecular biology of the cell

authors： Burakov A,Vorobjev I,Semenova I,Cowan A,Carson J,Wu Y,Rodionov V

更新日期：2021-01-13 00:00:00

abstract：:Activation of the inflammatory response is accompanied by a metabolic shift to aerobic glycolysis. Here we identify histone deacetylase 4 (HDAC4) as a new component of the immunometabolic program. We show that HDAC4 is required for efficient inflammatory cytokine production activated by lipopolysaccharide (LPS). Surpr...

journal_title：Molecular biology of the cell

authors： Wang B,Liu TY,Lai CH,Rao YH,Choi MC,Chi JT,Dai JW,Rathmell JC,Yao TP

更新日期：2014-11-01 00:00:00

abstract：:Vascular integrity and the maintenance of blood vessel continuity are fundamental features of the circulatory system maintained through endothelial cell-cell junctions. Defects in the endothelial barrier become an initiating factor in several pathologies, including ischemia/reperfusion, tumor angiogenesis, pulmonary e...

journal_title：Molecular biology of the cell

authors： Birukova AA,Singleton PA,Gawlak G,Tian X,Mirzapoiazova T,Mambetsariev B,Dubrovskyi O,Oskolkova OV,Bochkov VN,Birukov KG

更新日期：2014-07-01 00:00:00

abstract：:Polycystin (PC)1 and PC2 are membrane proteins implicated in autosomal dominant polycystic kidney disease. A physiologically relevant cleavage at PC1's G protein-coupled receptor proteolytic site (GPS) occurs early in the secretory pathway. Our results suggest that PC2 increases both PC1 GPS cleavage and PC1's appeara...

journal_title：Molecular biology of the cell

authors： Chapin HC,Rajendran V,Caplan MJ

更新日期：2010-12-01 00:00:00

abstract：:Candida albicans must undergo a switch from white to opaque to mate. Opaque cells then release mating type-specific pheromones that induce mating responses in opaque cells. Uniquely in C. albicans, the same pheromones induce mating-incompetent white cells to become cohesive, form an adhesive basal layer of cells on a ...

journal_title：Molecular biology of the cell

authors： Yi S,Sahni N,Daniels KJ,Pujol C,Srikantha T,Soll DR

更新日期：2008-03-01 00:00:00

abstract：:Cell invasion targets specific tissues in physiological placental implantation and pathological metastasis, which raises questions about how this process is controlled. We compare dermis and endometrium capacities to support trophoblast invasion, using matching sets of human primary fibroblasts in a coculture assay wi...

journal_title：Molecular biology of the cell

authors： Lopez-Mejia IC,De Toledo M,Della Seta F,Fafet P,Rebouissou C,Deleuze V,Blanchard JM,Jorgensen C,Tazi J,Vignais ML

更新日期：2013-10-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts wit...

journal_title：Molecular biology of the cell

authors： Newpher TM,Idrissi FZ,Geli MI,Lemmon SK

更新日期：2006-10-01 00:00:00

abstract：:Preventing polyspermy during animal fertilization relies on modifications to the egg's extracellular matrix. On fertilization in sea urchins, the contents of cortical granules are secreted and rapidly assemble into the egg's extracellular vitelline layer, forming the fertilization envelope, a proteinaceous structure t...

journal_title：Molecular biology of the cell

authors： Wong JL,Wessel GM

更新日期：2006-12-01 00:00:00

abstract：:The c-myb proto-oncogene product (c-Myb) regulates proliferation and differentiation of hematopoietic cells. Recently we have shown that c-Myb is degraded in response to Wnt-1 stimulation via a pathway involving TAK1 (TGF-beta-activated kinase), HIPK2 (homeodomain-interacting protein kinase 2), and NLK (Nemo-like kina...

journal_title：Molecular biology of the cell

authors： Kurahashi T,Nomura T,Kanei-Ishii C,Shinkai Y,Ishii S

更新日期：2005-10-01 00:00:00

abstract：:Based on its shedding and binding activities, the disintegrin and metalloprotease 12 (ADAM12) has been implicated in cell signaling. Here we investigate the intracellular protein interaction network of the transmembrane ADAM12L variant using an integrative approach. We identify the integrin-linked kinase (ILK) as a ne...

journal_title：Molecular biology of the cell

authors： Leyme A,Bourd-Boittin K,Bonnier D,Falconer A,Arlot-Bonnemains Y,Théret N

更新日期：2012-09-01 00:00:00

abstract：:Integrin alpha 3A cytoplasmic tail phosphorylation was mapped to amino acid S1042, as determined by mass spectrometry, and confirmed by mutagenesis. This residue occurs within a "QPSXXE" motif conserved in multiple alpha chains (alpha 3A, alpha 6A, alpha 7A), from multiple species. Phosphorylation of alpha 3A and alph...

journal_title：Molecular biology of the cell

authors： Zhang XA,Bontrager AL,Stipp CS,Kraeft SK,Bazzoni G,Chen LB,Hemler ME

更新日期：2001-02-01 00:00:00

abstract：:Podosomes are dynamic actin-rich structures composed of a dense F-actin core surrounded by a cloud of more diffuse F-actin. Src performs one or more unique functions in osteoclasts (OCLs), and podosome belts and bone resorption are impaired in the absence of Src. Using Src(-/-) OCLs, we investigated the specific funct...

journal_title：Molecular biology of the cell

authors： Destaing O,Sanjay A,Itzstein C,Horne WC,Toomre D,De Camilli P,Baron R

更新日期：2008-01-01 00:00:00

abstract：:To further elucidate the functions of actin in budding yeast and to relate actin structure to specific roles and interactions in vivo, we determined the phenotypes caused by 13 charged-to-alanine mutations isolated previously in the single Saccharomyces cerevisiae actin gene. Defects in actin organization, morphogenes...

journal_title：Molecular biology of the cell

authors： Drubin DG,Jones HD,Wertman KF

更新日期：1993-12-01 00:00:00

abstract：:Cilia serve as sensory devices in a diversity of organisms and their defects contribute to many human diseases. In primary cilia of kidney cells, the transient receptor potential polycystin (TRPP) channels polycystin-1 (PC-1) and polycystin-2 (PC-2) act as a mechanosensitive channel, with defects resulting in autosoma...

journal_title：Molecular biology of the cell

authors： Hu J,Bae YK,Knobel KM,Barr MM

更新日期：2006-05-01 00:00:00

abstract：:Calreticulin (CRT) is a high-capacity, low-affinity Ca2+-binding protein located in the lumen of the endoplasmic reticulum (ER) of all eukaryotic cells investigated so far. Its high level of conservation among different species suggests that it serves functions fundamental to cell survival. The role originally propose...

journal_title：Molecular biology of the cell

authors： Fasolato C,Pizzo P,Pozzan T

更新日期：1998-06-01 00:00:00

abstract：:The homotypic fusion of yeast vacuoles requires the Rab-family GTPase Ypt7p and its effector complex, homotypic fusion and vacuole protein sorting complex (HOPS). Although the vacuolar kinase Yck3p is required for the sensitivity of vacuole fusion to proteins that regulate the Rab GTPase cycle-Gdi1p (GDP-dissociation ...

journal_title：Molecular biology of the cell

authors： Zick M,Wickner W

更新日期：2012-09-01 00:00:00

abstract：:Long-range chromatin interactions between gene loci in the cell nucleus are important for many biological processes, including transcriptional regulation. Previously, we demonstrated that several genes specifically cluster with the astrocyte-specific gene for glial fibrillary acidic protein (Gfap) during astrocyte dif...

journal_title：Molecular biology of the cell

authors： Ito K,Noguchi A,Uosaki Y,Taga T,Arakawa H,Takizawa T

更新日期：2018-01-15 00:00:00

abstract：:Topogenic determinants that direct protein topology at the endoplasmic reticulum membrane usually function with high fidelity to establish a uniform topological orientation for any given polypeptide. Here we show, however, that through the coupling of sequential translocation events, native topogenic determinants are ...

journal_title：Molecular biology of the cell

authors： Moss K,Helm A,Lu Y,Bragin A,Skach WR

更新日期：1998-09-01 00:00:00