Phosphorylation of a conserved integrin alpha 3 QPSXXE motif regulates signaling, motility, and cytoskeletal engagement.

abstract：:Oncoprotein 18/stathmin (Op18) has been identified recently as a protein that destabilizes microtubules, but the mechanism of destabilization is currently controversial. Based on in vitro microtubule assembly assays, evidence has been presented supporting conflicting destabilization models of either tubulin sequestrat...

journal_title：Molecular biology of the cell

authors： Howell B,Larsson N,Gullberg M,Cassimeris L

更新日期：1999-01-01 00:00:00

abstract：:We have shown previously that interleukin-4 (IL-4) protects TS1alphabeta cells from apoptosis, but very little is known about the mechanism by which IL-4 exerts this effect. We found that Akt activity, which is dependent on phosphatidylinositol 3 kinase, is reduced in IL-4-deprived TS1alphabeta cells. Overexpression o...

journal_title：Molecular biology of the cell

authors： Cerezo A,Martínez-A C,Lanzarot D,Fischer S,Franke TF,Rebollo A

更新日期：1998-11-01 00:00:00

abstract：:Eukaryotic cells eliminate misfolded proteins from the endoplasmic reticulum (ER) via a conserved process termed ER-associated degradation (ERAD). Central regulators of the ERAD system are membrane-bound ubiquitin ligases, which are thought to channel misfolded proteins through the ER membrane during retrotranslocatio...

journal_title：Molecular biology of the cell

authors： Zhang T,Xu Y,Liu Y,Ye Y

更新日期：2015-12-01 00:00:00

abstract：:Although p38 activity is reported to be required as cells enter mitosis for proper spindle assembly and checkpoint function, its role during the division process remains controversial in lieu of direct data. We therefore conducted live cell studies to determine the effect on mitosis of inhibiting or depleting p38. We ...

journal_title：Molecular biology of the cell

authors： Lee K,Kenny AE,Rieder CL

更新日期：2010-07-01 00:00:00

abstract：:Telomere dysfunction results in fertility defects in a number of organisms. Although data from fission yeast and Caenorhabditis elegans suggests that telomere dysfunction manifests itself primarily as defects in proper meiotic chromosome segregation, it is unclear how mammalian telomere dysfunction results in germ cel...

journal_title：Molecular biology of the cell

authors： Hemann MT,Rudolph KL,Strong MA,DePinho RA,Chin L,Greider CW

更新日期：2001-07-01 00:00:00

abstract：:Androgen receptor (AR) signaling in Sertoli cells is known to be important for germ-cell progression through meiosis, but the extent to which androgens indirectly regulate specific meiotic stages is not known. Here, we combine synchronization of spermatogenesis, cytological analyses and single-cell RNAseq (scRNAseq) i...

journal_title：Molecular biology of the cell

authors： Larose H,Kent T,Ma Q,Shami AN,Harerimana N,Li JZ,Hammoud SS,Handel MA

更新日期：2020-12-01 00:00:00

abstract：:Mitochondrial dysfunction is associated with the development of many age-related human diseases. Therefore recognizing and correcting the early signs of malfunctioning mitochondria is of critical importance for cellular welfare and survival. We previously demonstrated that VCP/Cdc48-associated mitochondrial stress res...

journal_title：Molecular biology of the cell

authors： Heo JM,Nielson JR,Dephoure N,Gygi SP,Rutter J

更新日期：2013-05-01 00:00:00

abstract：:We observed the dynamic recruitment of spindle checkpoint proteins Mad1 and Bub1 to detached kinetochores in budding yeast using real-time live-cell imaging and quantified recruitment in fixed cells. After induced de novo kinetochore assembly at one pair of sister centromeres, Mad1 appeared after the kinetochore prote...

journal_title：Molecular biology of the cell

authors： Krefman NI,Drubin DG,Barnes G

更新日期：2015-07-15 00:00:00

abstract：:We have shown previously that the transforming growth factor-beta (TGFbeta)-regulated Sma-Mad (Smad) protein 3 and Smad4 proteins transactivate the apolipoprotein C-III promoter in hepatic cells via a hormone response element that binds the nuclear receptor hepatocyte nuclear factor 4 (HNF-4). In the present study, we...

journal_title：Molecular biology of the cell

authors： Chou WC,Prokova V,Shiraishi K,Valcourt U,Moustakas A,Hadzopoulou-Cladaras M,Zannis VI,Kardassis D

更新日期：2003-03-01 00:00:00

abstract：:Signaling via the Ras pathway involves sequential activation of Ras, Raf-1, mitogen-activated protein kinase kinase (MKK), and the extracellular signal-regulated (ERK) group of mitogen-activated protein (MAP) kinases. Expression from the c-Fos, atrial natriuretic factor (ANF), and myosin light chain-2 (MLC-2) promoter...

journal_title：Molecular biology of the cell

authors： Thorburn J,Carlson M,Mansour SJ,Chien KR,Ahn NG,Thorburn A

更新日期：1995-11-01 00:00:00

abstract：:A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of th...

journal_title：Molecular biology of the cell

authors： Midwood KS,Schwarzbauer JE

更新日期：2002-10-01 00:00:00

abstract：:The formin mDia2 mediates the formation of lamellipodia and filopodia during cell locomotion. The subcellular localization of activated mDia2 depends on interactions with actin filaments and the plasma membrane. We investigated the poorly understood mechanism of plasma membrane targeting of mDia2 and found that the en...

journal_title：Molecular biology of the cell

authors： Gorelik R,Yang C,Kameswaran V,Dominguez R,Svitkina T

更新日期：2011-01-15 00:00:00

abstract：:A central question in the area of signal transduction is why pathways utilize common components. In the budding yeast Saccharomyces cerevisiae, the HOG and filamentous growth (FG) MAPK pathways require overlapping components but are thought to be induced by different stimuli and specify distinct outputs. To better und...

journal_title：Molecular biology of the cell

authors： Pitoniak A,Birkaya B,Dionne HM,Vadaie N,Cullen PJ

更新日期：2009-07-01 00:00:00

abstract：:Clustered protocadherins (Pcdhs) are arranged in gene clusters (α, β, and γ) with variable and constant exons. Variable exons encode cadherin and transmembrane domains and ~90 cytoplasmic residues. The 14 Pcdh-αs and 22 Pcdh-γs are spliced to constant exons, which, for Pcdh-γs, encode ~120 residues of an identical cyt...

journal_title：Molecular biology of the cell

authors： O'Leary R,Reilly JE,Hanson HH,Kang S,Lou N,Phillips GR

更新日期：2011-11-01 00:00:00

abstract：:The Rho GTPases RhoA and Rac1 function as master regulators of cytokinesis by controlling the actomyosin cytoskeleton. RhoA and Rac1 have to be respectively activated and inactivated at the division plane for cytokinesis to occur properly. The inactivation of Rac1 at the cleavage furrow is controlled by MgcRacGAP. How...

journal_title：Molecular biology of the cell

authors： Cannet A,Schmidt S,Delaval B,Debant A

更新日期：2014-12-15 00:00:00

abstract：:Significant increase in JNK, c-Jun, and Cdk5 activities are reported in Alzheimer's disease (AD). Inhibition of c-Jun prevents neuronal cell death in in vivo AD models, highlighting it as a major JNK effector. Both JNK and Cdk5 promote neurodegeneration upon deregulation; however, Cdk5 has not been mechanistically lin...

journal_title：Molecular biology of the cell

authors： Sun KH,Lee HG,Smith MA,Shah K

更新日期：2009-11-01 00:00:00

abstract：:In many organisms nonsense mutations decrease the level of mRNA. In the case of mammalian cells, it is still controversial whether translation is required for this nonsense-mediated RNA decrease (NMD). Although previous analyzes have shown that conditions that impede translation termination at nonsense codons also pre...

journal_title：Molecular biology of the cell

authors： Buzina A,Shulman MJ

更新日期：1999-03-01 00:00:00

abstract：:During the cell cycle, Cdc2-cyclin B kinase abruptly becomes active and triggers the entry into mitosis/meiosis. Recently, it was found that inactive Cdc2-cyclin B is present in aggregates in immature starfish oocytes and becomes disaggregated at the time of its activation during maturation. We discuss a possible scen...

journal_title：Molecular biology of the cell

authors： Slepchenko BM,Terasaki M

更新日期：2003-11-01 00:00:00

abstract：:Rab GTPases are localized to various intracellular compartments and are known to play important regulatory roles in membrane trafficking. Here, we report the subcellular distribution and function of Rab14. By immunofluorescence and immunoelectron microscopy, both endogenous as well as overexpressed Rab14 were localize...

journal_title：Molecular biology of the cell

authors： Junutula JR,De Maziére AM,Peden AA,Ervin KE,Advani RJ,van Dijk SM,Klumperman J,Scheller RH

更新日期：2004-05-01 00:00:00

abstract：:Cell shape is regulated by cell adhesion and cytoskeletal and membrane dynamics. Cell shape, adhesion, and motility have a complex relationship and understanding them is important in understanding developmental patterning and embryogenesis. Here we show that the lipid kinase phosphatidylinositol 4-kinase III beta (PI4...

journal_title：Molecular biology of the cell

authors： Bilodeau P,Jacobsen D,Law-Vinh D,Lee JM

更新日期：2020-08-01 00:00:00

abstract：:Capture of newly synthesized proteins into endoplasmic reticulum (ER)-derived coat protomer type II (COPII) vesicles represents a critical juncture in the quality control of protein biogenesis within the secretory pathway. The yeast ATP-binding cassette transporter Yor1p is a pleiotropic drug pump that shows homology ...

journal_title：Molecular biology of the cell

authors： Pagant S,Kung L,Dorrington M,Lee MC,Miller EA

更新日期：2007-09-01 00:00:00

abstract：:Cellular differentiation and lineage commitment are considered to be robust and irreversible processes during development. Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four transcription factors. We hypothesized that combinatorial expression of...

journal_title：Molecular biology of the cell

authors： Ishii R,Kami D,Toyoda M,Makino H,Gojo S,Ishii T,Umezawa A

更新日期：2012-09-01 00:00:00

abstract：:Previous work has established that activation of Mos, Mek, and p42 mitogen-activated protein (MAP) kinase can trigger release from G2-phase arrest in Xenopus oocytes and oocyte extracts and can cause Xenopus embryos and extracts to arrest in mitosis. Herein we have found that activation of the MAP kinase cascade can a...

journal_title：Molecular biology of the cell

authors： Walter SA,Guadagno TM,Ferrell JE Jr

更新日期：1997-11-01 00:00:00

abstract：:Tumor cells use actin-rich protrusions called invadopodia to degrade extracellular matrix (ECM) and invade tissues; related structures, termed podosomes, are sites of dynamic ECM interaction. We show here that supervillin (SV), a peripheral membrane protein that binds F-actin and myosin II, reorganizes the actin cytos...

journal_title：Molecular biology of the cell

authors： Crowley JL,Smith TC,Fang Z,Takizawa N,Luna EJ

更新日期：2009-02-01 00:00:00

abstract：:In mammalian cells, the cortical endoplasmic reticulum (cER) is a network of tubules and cisterns that lie in close apposition to the plasma membrane (PM). We provide evidence that PM domains enriched in underlying cER function as trafficking hubs for insertion and removal of PM proteins in HEK 293 cells. By simultane...

journal_title：Molecular biology of the cell

authors： Fox PD,Haberkorn CJ,Weigel AV,Higgins JL,Akin EJ,Kennedy MJ,Krapf D,Tamkun MM

更新日期：2013-09-01 00:00:00

abstract：:The yeast SIR2 gene and many of its homologs have been identified as NAD(+)-dependent histone deacetylases. To get a broader view of the relationship between the histone deacetylase activity of Sir2p and its in vivo functions we have mutated eight highly conserved residues in the core domain of SIR2. These mutations h...

journal_title：Molecular biology of the cell

authors： Armstrong CM,Kaeberlein M,Imai SI,Guarente L

更新日期：2002-04-01 00:00:00

abstract：:Phosphatidylinositol 4, 5-bisphosphate (PIP(2)) at the inner leaflet of the plasma membrane has been proposed to locally regulate the actin cytoskeleton. Indeed, recent studies that use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent PIP(2) sensors suggest that this lipid is enriched in membrane microdo...

journal_title：Molecular biology of the cell

authors： van Rheenen J,Jalink K

更新日期：2002-09-01 00:00:00

abstract：:Caveolae are vesicular invaginations of the plasma membrane. Caveolin-3 is the principal structural component of caveolae in skeletal muscle cells in vivo. We have recently generated caveolin-3 transgenic mice and demonstrated that overexpression of wild-type caveolin-3 in skeletal muscle fibers is sufficient to induc...

journal_title：Molecular biology of the cell

authors： Volonte D,Peoples AJ,Galbiati F

更新日期：2003-10-01 00:00:00

abstract：:The Ste5 scaffold activates an associated mitogen-activated protein kinase cascade by binding through its RING-H2 domain to a Gbetagamma dimer (Ste4/Ste18) at the plasma membrane in a recruitment event that requires prior nuclear shuttling of Ste5. Genetic evidence suggests that Ste5 must oligomerize to function, but ...

journal_title：Molecular biology of the cell

authors： Wang Y,Elion EA

更新日期：2003-06-01 00:00:00

abstract：:To further elucidate the functions of actin in budding yeast and to relate actin structure to specific roles and interactions in vivo, we determined the phenotypes caused by 13 charged-to-alanine mutations isolated previously in the single Saccharomyces cerevisiae actin gene. Defects in actin organization, morphogenes...

journal_title：Molecular biology of the cell

authors： Drubin DG,Jones HD,Wertman KF

更新日期：1993-12-01 00:00:00