Abstract:
:In mammalian cells, the cortical endoplasmic reticulum (cER) is a network of tubules and cisterns that lie in close apposition to the plasma membrane (PM). We provide evidence that PM domains enriched in underlying cER function as trafficking hubs for insertion and removal of PM proteins in HEK 293 cells. By simultaneously visualizing cER and various transmembrane protein cargoes with total internal reflectance fluorescence microscopy, we demonstrate that the majority of exocytotic delivery events for a recycled membrane protein or for a membrane protein being delivered to the PM for the first time occur at regions enriched in cER. Likewise, we observed recurring clathrin clusters and functional endocytosis of PM proteins preferentially at the cER-enriched regions. Thus the cER network serves to organize the molecular machinery for both insertion and removal of cell surface proteins, highlighting a novel role for these unique cellular microdomains in membrane trafficking.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Fox PD,Haberkorn CJ,Weigel AV,Higgins JL,Akin EJ,Kennedy MJ,Krapf D,Tamkun MMdoi
10.1091/mbc.E12-12-0895subject
Has Abstractpub_date
2013-09-01 00:00:00pages
2703-13issue
17eissn
1059-1524issn
1939-4586pii
mbc.E12-12-0895journal_volume
24pub_type
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