Abstract:
:The yeast SIR2 gene and many of its homologs have been identified as NAD(+)-dependent histone deacetylases. To get a broader view of the relationship between the histone deacetylase activity of Sir2p and its in vivo functions we have mutated eight highly conserved residues in the core domain of SIR2. These mutations have a range of effects on the ability of Sir2p to deacetylate histones in vitro and to silence genes at the telomeres and HM loci. Interestingly, there is not a direct correlation between the in vitro and in vivo effects in some of these mutations. We also show that the histone deacetylase activity of Sir2p is necessary for the proper localiztion of the SIR complex to the telomeres.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Armstrong CM,Kaeberlein M,Imai SI,Guarente Ldoi
10.1091/mbc.01-10-0482keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
1427-38issue
4eissn
1059-1524issn
1939-4586journal_volume
13pub_type
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