Abstract:
:Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outstanding questions. Here we show, using proteomic, cytological, and functional analyses, that autophagosomes are spatially, physically, and functionally linked to endoplasmic reticulum exit sites (ERES), which are specialized regions of the endoplasmic reticulum where COPII transport vesicles are generated. Our data demonstrate that ERES are core autophagosomal biogenesis components whose function is required for the hierarchical assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex at phagophore assembly sites.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Graef M,Friedman JR,Graham C,Babu M,Nunnari Jdoi
10.1091/mbc.E13-07-0381subject
Has Abstractpub_date
2013-09-01 00:00:00pages
2918-31issue
18eissn
1059-1524issn
1939-4586pii
mbc.E13-07-0381journal_volume
24pub_type
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