The role of the Src homology domains in morphological transformation by v-src.


:The Src homology (SH2 and SH3) domains of v-Src are required for transformation of Rat-2 cells and for wild-type (morphr) transformation of chicken embryo fibroblasts (CEFs). We report herein that the N-terminal domains of v-Src, when expressed in trans, cannot complement the transformation defect of a deletion mutant lacking the "unique," SH3, and SH2 regions. However, the same regions of Src can promote transformation when translocated to the C terminus of v-Src, although the transformation of CEFs is somewhat slower. We conclude that the SH3 and SH2 domains must be present in cis to the catalytic domain to promote transformation but that transformation is not dependent on the precise intramolecular location of these domains. In CEFSs and in Rat-2 cells, the expression of wild-type v-Src results in tyrosine phosphorylation of proteins that bind to the v-Src SH3 and SH2 domains in vitro; mutations in the SH2 or SH3 and SH2 domains prevent the phosphorylation of these proteins. These findings are most consistent with models in which the SH3 and SH2 domains of v-Src directly or indirectly target the catalytic domain to substrates involved in transformation. However, the N-terminal domains of v-Src can promote tyrosine phosphorylation of certain proteins, in particular p130Cas, even when expressed in the absence of the catalytic domain, indicating that the N-terminal domains of v-Src have effects that are independent of the catalytic domain.


Mol Biol Cell


Tian M,Martin GS




Has Abstract


1997-07-01 00:00:00












  • Analysis of the roles of phosphatidylinositol-4,5-bisphosphate and individual subunits in assembly, localization, and function of Saccharomyces cerevisiae target of rapamycin complex 2.

    abstract::Eukaryotic cell survival requires maintenance of plasma membrane (PM) homeostasis in response to environmental insults and changes in lipid metabolism. In yeast, a key regulator of PM homeostasis is target of rapamycin (TOR) complex 2 (TORC2), a multiprotein complex containing the evolutionarily conserved TOR protein ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Martinez Marshall MN,Emmerstorfer-Augustin A,Leskoske KL,Zhang LH,Li B,Thorner J

    更新日期:2019-06-01 00:00:00

  • Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit.

    abstract::DSL1 was identified through its genetic interaction with SLY1, which encodes a t-SNARE-interacting protein that functions in endoplasmic reticulum (ER)-to-Golgi traffic. Conditional dsl1 mutants exhibit a block in ER-to-Golgi traffic at the restrictive temperature. Here, we show that dsl1 mutants are defective for ret...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Reilly BA,Kraynack BA,VanRheenen SM,Waters MG

    更新日期:2001-12-01 00:00:00

  • Chlamydomonas IFT70/CrDYF-1 is a core component of IFT particle complex B and is required for flagellar assembly.

    abstract::DYF-1 is a highly conserved protein essential for ciliogenesis in several model organisms. In Caenorhabditis elegans, DYF-1 serves as an essential activator for an anterograde motor OSM-3 of intraflagellar transport (IFT), the ciliogenesis-required motility that mediates the transport of flagellar precursors and remov...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Fan ZC,Behal RH,Geimer S,Wang Z,Williamson SM,Zhang H,Cole DG,Qin H

    更新日期:2010-08-01 00:00:00

  • Myo1c binds phosphoinositides through a putative pleckstrin homology domain.

    abstract::Myo1c is a member of the myosin superfamily that binds phosphatidylinositol-4,5-bisphosphate (PIP(2)), links the actin cytoskeleton to cellular membranes and plays roles in mechano-signal transduction and membrane trafficking. We located and characterized two distinct membrane binding sites within the regulatory and t...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Hokanson DE,Laakso JM,Lin T,Sept D,Ostap EM

    更新日期:2006-11-01 00:00:00

  • Mutant rab8 Impairs docking and fusion of rhodopsin-bearing post-Golgi membranes and causes cell death of transgenic Xenopus rods.

    abstract::Rab8 is a GTPase involved in membrane trafficking. In photoreceptor cells, rab8 is proposed to participate in the late stages of delivery of rhodopsin-containing post-Golgi membranes to the plasma membrane near the base of the connecting cilium. To test the function of rab8 in vivo, we generated transgenic Xenopus lae...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Moritz OL,Tam BM,Hurd LL,Peränen J,Deretic D,Papermaster DS

    更新日期:2001-08-01 00:00:00

  • Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bernis C,Swift-Taylor B,Nord M,Carmona S,Chook YM,Forbes DJ

    更新日期:2014-04-01 00:00:00

  • Autocrine transforming growth factor-{beta}1 activation mediated by integrin {alpha}V{beta}3 regulates transcriptional expression of laminin-332 in Madin-Darby canine kidney epithelial cells.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Moyano JV,Greciano PG,Buschmann MM,Koch M,Matlin KS

    更新日期:2010-11-01 00:00:00

  • Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Shih JL,Reck-Peterson SL,Newitt R,Mooseker MS,Aebersold R,Herskowitz I

    更新日期:2005-10-01 00:00:00

  • Cyclin A- and cyclin E-Cdk complexes shuttle between the nucleus and the cytoplasm.

    abstract::Cyclins A and E and their partner cyclin-dependent kinases (Cdks) are key regulators of DNA synthesis and of mitosis. Immunofluorescence studies have shown that both cyclins are nuclear and that a proportion of cyclin A is localized to sites of DNA replication. However, recently, both cyclin A and cyclin E have been i...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Jackman M,Kubota Y,den Elzen N,Hagting A,Pines J

    更新日期:2002-03-01 00:00:00

  • Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Conibear E,Stevens TH

    更新日期:2000-01-01 00:00:00

  • Energy metabolism regulates clathrin adaptors at the trans-Golgi network and endosomes.

    abstract::Glucose is a master regulator of cell behavior in the yeast Saccharomyces cerevisiae. It acts as both a metabolic substrate and a potent regulator of intracellular signaling cascades. Glucose starvation induces the transient delocalization and then partial relocalization of clathrin adaptors at the trans-Golgi network...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Aoh QL,Hung CW,Duncan MC

    更新日期:2013-03-01 00:00:00

  • Exposure of bipartite hydrophobic signal triggers nuclear quality control of Ndc10 at the endoplasmic reticulum/nuclear envelope.

    abstract::Proper functioning of the protein-folding quality control network depends on the network's ability to discern diverse structural perturbations to the native states of its protein substrates. Despite the centrality of the detection of misfolded states to cell home-ostasis, very little is known about the exact sequence ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Furth N,Gertman O,Shiber A,Alfassy OS,Cohen I,Rosenberg MM,Doron NK,Friedler A,Ravid T

    更新日期:2011-12-01 00:00:00

  • N-glycosylation-dependent regulation of hK2P17.1 currents.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Wiedmann F,Schlund D,Voigt N,Ratte A,Kraft M,Katus HA,Schmidt C

    更新日期:2019-06-01 00:00:00

  • Phosphorylation of the autoinhibitory domain of the Sso t-SNAREs promotes binding of the Vsm1 SNARE regulator in yeast.

    abstract::We have shown that protein kinase A phosphorylation of t-SNAREs inhibits SNARE assembly and suppresses endo- and exocytosis in yeast. Herein, we show that protein kinase A phosphorylation of the Sso exocytic t-SNAREs promotes the binding of Vsm1, a potential SNARE regulator identified previously in our laboratory. Pho...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Marash M,Gerst JE

    更新日期:2003-08-01 00:00:00

  • Connexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+]i responses.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Schalper KA,Palacios-Prado N,Retamal MA,Shoji KF,Martínez AD,Sáez JC

    更新日期:2008-08-01 00:00:00

  • Myo6 facilitates the translocation of endocytic vesicles from cell peripheries.

    abstract::Immunolocalization studies in epithelial cells revealed myo6 was associated with peripherally located vesicles that contained the transferrin receptor. Pulse-chase experiments after transferrin uptake showed that these vesicles were newly uncoated endocytic vesicles and that myo6 was recruited to these vesicles immedi...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Aschenbrenner L,Lee T,Hasson T

    更新日期:2003-07-01 00:00:00

  • Efficient interaction between two GTPases allows the chloroplast SRP pathway to bypass the requirement for an SRP RNA.

    abstract::Cotranslational protein targeting to membranes is regulated by two GTPases in the signal recognition particle (SRP) and the SRP receptor; association between the two GTPases is slow and is accelerated 400-fold by the SRP RNA. Intriguingly, the otherwise universally conserved SRP RNA is missing in a novel chloroplast S...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Jaru-Ampornpan P,Chandrasekar S,Shan SO

    更新日期:2007-07-01 00:00:00

  • The cytoplasmic chaperone hsp104 is required for conformational repair of heat-denatured proteins in the yeast endoplasmic reticulum.

    abstract::Severe heat stress causes protein denaturation in various cellular compartments. If Saccharomyces cerevisiae cells grown at 24 degrees C are preconditioned at 37 degrees C, proteins denatured by subsequent exposure to 48-50 degrees C can be renatured when the cells are allowed to recover at 24 degrees C. Conformationa...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Hänninen AL,Simola M,Saris N,Makarow M

    更新日期:1999-11-01 00:00:00

  • Translationally repressed mRNA transiently cycles through stress granules during stress.

    abstract::In mammals, repression of translation during stress is associated with the assembly of stress granules in the cytoplasm, which contain a fraction of arrested mRNA and have been proposed to play a role in their storage. Because physical contacts are seen with GW bodies, which contain the mRNA degradation machinery, str...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Mollet S,Cougot N,Wilczynska A,Dautry F,Kress M,Bertrand E,Weil D

    更新日期:2008-10-01 00:00:00

  • TOR1 and TOR2 are structurally and functionally similar but not identical phosphatidylinositol kinase homologues in yeast.

    abstract::The Saccharomyces cerevisiae genes TOR1 and TOR2 were originally identified by mutations that confer resistance to the immunosuppressant rapamycin. TOR2 was previously shown to encode an essential 282-kDa phosphatidylinositol kinase (PI kinase) homologue. The TOR1 gene product is also a large (281 kDa) PI kinase homol...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Helliwell SB,Wagner P,Kunz J,Deuter-Reinhard M,Henriquez R,Hall MN

    更新日期:1994-01-01 00:00:00

  • An aspartyl cathepsin, CTH3, is essential for proprotein processing during secretory granule maturation in Tetrahymena thermophila.

    abstract::In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key rol...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kumar S,Briguglio JS,Turkewitz AP

    更新日期:2014-08-15 00:00:00

  • Interallelic complementation provides functional evidence for cohesin-cohesin interactions on DNA.

    abstract::The cohesin complex (Mcd1p, Smc1p, Smc3p, and Scc3p) has multiple roles in chromosome architecture, such as promoting sister chromatid cohesion, chromosome condensation, DNA repair, and transcriptional regulation. The prevailing embrace model for sister chromatid cohesion posits that a single cohesin complex entraps b...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Eng T,Guacci V,Koshland D

    更新日期:2015-11-15 00:00:00

  • The septin-associated kinase Gin4 recruits Gps1 to the site of cell division.

    abstract::Cell cycle-dependent morphogenesis of unicellular organisms depends on the spatiotemporal control of cell polarity. Rho GTPases are the major players that guide cells through structural reorganizations such as cytokinesis (Rho1 dependent) and polarity establishment (Cdc42 dependent). In budding yeast, the protein Gps1...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Meitinger F,Pereira G

    更新日期:2017-04-01 00:00:00

  • Condensin suppresses recombination and regulates double-strand break processing at the repetitive ribosomal DNA array to ensure proper chromosome segregation during meiosis in budding yeast.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Li P,Jin H,Yu HG

    更新日期:2014-10-01 00:00:00

  • Locus-specific gene repositioning in prostate cancer.

    abstract::Genes occupy preferred spatial positions within interphase cell nuclei. However, positioning patterns are not an innate feature of a locus, and genes can alter their localization in response to physiological and pathological changes. Here we screen the radial positioning patterns of 40 genes in normal, hyperplasic, an...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Leshner M,Devine M,Roloff GW,True LD,Misteli T,Meaburn KJ

    更新日期:2016-01-15 00:00:00

  • Cell cycle-regulated trafficking of Chs2 controls actomyosin ring stability during cytokinesis.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: VerPlank L,Li R

    更新日期:2005-05-01 00:00:00

  • Autocatalytic processing of m-AAA protease subunits in mitochondria.

    abstract::m-AAA proteases are ATP-dependent proteolytic machines in the inner membrane of mitochondria which are crucial for the maintenance of mitochondrial activities. Conserved nuclear-encoded subunits, termed paraplegin, Afg3l1, and Afg3l2, form various isoenzymes differing in their subunit composition in mammalian mitochon...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Koppen M,Bonn F,Ehses S,Langer T

    更新日期:2009-10-01 00:00:00

  • What makes the cell cycle tick? a celebration of the awesome power of biochemistry and the frog egg.

    abstract::The cell cycle, a 19th century discovery of cytologists, only achieved a satisfactory biochemical explanation in the last 20 years of the 20th century. This personal retrospective focuses on how biochemical studies of the frog egg helped identify the cyclin-based mitotic oscillator and how this approach quickly merged...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kirschner M

    更新日期:2020-12-15 00:00:00

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Greco V

    更新日期:2016-11-01 00:00:00

  • Autoinhibition of Jak2 tyrosine kinase is dependent on specific regions in its pseudokinase domain.

    abstract::Jak tyrosine kinases have a unique domain structure containing a kinase domain (JH1) adjacent to a catalytically inactive pseudokinase domain (JH2). JH2 is crucial for inhibition of basal Jak activity, but the mechanism of this regulation has remained elusive. We show that JH2 negatively regulated Jak2 in bacterial ce...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Saharinen P,Vihinen M,Silvennoinen O

    更新日期:2003-04-01 00:00:00