The link between autophagy and secretion: a story of multitasking proteins.

abstract：:Basal bodies organize cilia that are responsible for both mechanical beating and sensation. Nascent basal body assembly follows a series of well characterized morphological events; however, the proteins and their assembly dynamics for new basal body formation and function are not well understood. High-resolution light...

journal_title：Molecular biology of the cell

authors： Pearson CG,Giddings TH Jr,Winey M

更新日期：2009-02-01 00:00:00

abstract：:The Aurora kinase complex, also called the chromosomal passenger complex (CPC), is essential for faithful chromosome segregation and completion of cell division. In Fungi and Animalia, this complex consists of the kinase Aurora B/AIR-2/Ipl1p, INCENP/ICP-1/Sli15p, and Survivin/BIR-1/Bir1p. A fourth subunit, Borealin/Da...

journal_title：Molecular biology of the cell

authors： Nakajima Y,Tyers RG,Wong CC,Yates JR 3rd,Drubin DG,Barnes G

更新日期：2009-03-01 00:00:00

abstract：:Since 2008, the autophagy community has periodically published a set of guidelines, currently titled "Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy." The newest version of the guidelines was published in 2016. There are many reasons for establishing a set of guidelines in a given researc...

journal_title：Molecular biology of the cell

authors： Klionsky DJ

更新日期：2016-03-01 00:00:00

abstract：:We report the identification of Cdc7/Dbf4 phosphorylation sites in human MCM2 and the determination of the role of Cdc7/Dbf4 phosphorylation of MCM2 in the initiation of DNA replication. Using immunoblotting, immunofluorescence, and high-speed automated cell-imaging analyses with antibodies specific against MCM2 and C...

journal_title：Molecular biology of the cell

authors： Tsuji T,Ficarro SB,Jiang W

更新日期：2006-10-01 00:00:00

abstract：:The yeast Smf1p Nramp manganese transporter is posttranslationally regulated by environmental manganese. Smf1p is stabilized at the cell surface with manganese starvation, but is largely degraded in the vacuole with physiological manganese through a mechanism involving the Rsp5p adaptor complex Bsd2p/Tre1p/Tre2p. We n...

journal_title：Molecular biology of the cell

authors： Jensen LT,Carroll MC,Hall MD,Harvey CJ,Beese SE,Culotta VC

更新日期：2009-06-01 00:00:00

abstract：:Subcellular targeting and the activity of facilitative glucose transporters are likely to be regulated by interactions with cellular proteins. This report describes the identification and characterization of a protein, GLUT1 C-terminal binding protein (GLUT1CBP), that binds via a PDZ domain to the C terminus of GLUT1....

journal_title：Molecular biology of the cell

authors： Bunn RC,Jensen MA,Reed BC

更新日期：1999-04-01 00:00:00

abstract：:Growth cone responses to guidance cues provide the basis for neuronal pathfinding. Although many cues have been identified, less is known about how signals are translated into the cytoskeletal rearrangements that steer directional changes during pathfinding. Here we show that the response of dorsal root ganglion (DRG)...

journal_title：Molecular biology of the cell

authors： Brown JA,Wysolmerski RB,Bridgman PC

更新日期：2009-02-01 00:00:00

abstract：:Manganese (Mn) protects cells against lethal doses of purified Shiga toxin by causing the degradation of the cycling transmembrane protein GPP130, which the toxin uses as a trafficking receptor. Mn-induced GPP130 down-regulation, in addition to being a potential therapeutic approach against Shiga toxicosis, is a model...

journal_title：Molecular biology of the cell

authors： Tewari R,Jarvela T,Linstedt AD

更新日期：2014-10-01 00:00:00

abstract：:Normal epithelial cells undergo apoptosis when they are denied contact with the extracellular matrix, in a process termed "anoikis." Conversely, malignant epithelial cells typically acquire anchorage independence, i.e., the capacity to survive and grow in the absence of matrix interaction. Here we asked the question w...

journal_title：Molecular biology of the cell

authors： Jost M,Huggett TM,Kari C,Rodeck U

更新日期：2001-05-01 00:00:00

abstract：:Conformational diseases are associated with the conversion of normal proteins into aggregation-prone toxic conformers with structures similar to that of β-amyloid. Spatial distribution of amyloid-like proteins into intracellular quality control centers can be beneficial, but cellular mechanisms for protective aggregat...

journal_title：Molecular biology of the cell

authors： Wolfe KJ,Ren HY,Trepte P,Cyr DM

更新日期：2013-12-01 00:00:00

abstract：:Insulin maintains whole body blood glucose homeostasis, in part, by regulating the amount of the GLUT4 glucose transporter on the cell surface of fat and muscle cells. Insulin induces the redistribution of GLUT4 from intracellular compartments to the plasma membrane, by stimulating a large increase in exocytosis and a...

journal_title：Molecular biology of the cell

authors： Zeigerer A,McBrayer MK,McGraw TE

更新日期：2004-10-01 00:00:00

abstract：:Integrins are major receptors used by cells to interact with extracellular matrices. In this paper, we identify the first ligands for the beta 8 family of integrins, presenting evidence that integrin heterodimers containing the beta 8 subunit mediate interactions of chick sensory neurons with laminin-1, collagen IV, a...

journal_title：Molecular biology of the cell

authors： Venstrom K,Reichardt L

更新日期：1995-04-01 00:00:00

abstract：:Colony-stimulating factor-1 (CSF-1) induces expression of immediate early gene, such as c-myc and c-fos and delayed early genes such as D-type cyclins (D1 and D2), whose products play essential roles in the G1 to S phase transition of the cell cycle. Little is known, however, about the cytoplasmic signal transduction ...

journal_title：Molecular biology of the cell

authors： Dey A,She H,Kim L,Boruch A,Guris DL,Carlberg K,Sebti SM,Woodley DT,Imamoto A,Li W

更新日期：2000-11-01 00:00:00

abstract：:Chironomus tentans-repressor splicing factor (Ct-RSF) represses the activation of splicing by SR proteins in vitro. Ct-RSF colocalizes with the Ser-Arg-rich (SR) protein hrp45 in interchromatin granule clusters and coimmunoprecipitates with hrp45 in nuclear extracts. Ct-RSF and hrp45 can also interact directly in vitr...

journal_title：Molecular biology of the cell

authors： Björk P,Wetterberg-Strandh I,Baurén G,Wieslander L

更新日期：2006-01-01 00:00:00

abstract：:The second step of dolichol-linked oligosaccharide synthesis in the N-linked glycosylation pathway at the endoplasmic reticulum (ER) membrane is catalyzed by an unusual hetero-oligomeric UDP-N-acetylglucosamine transferase that in most eukaryotes is comprised of at least two subunits, Alg13p and Alg14p. Alg13p is the ...

journal_title：Molecular biology of the cell

authors： Averbeck N,Gao XD,Nishimura S,Dean N

更新日期：2008-05-01 00:00:00

abstract：:ATP8A2 is a P4-ATPase that flips phosphatidylserine and phosphatidylethanolamine across cell membranes. This generates membrane phospholipid asymmetry, a property important in many cellular processes, including vesicle trafficking. ATP8A2 deficiency causes severe neurodegenerative diseases. We investigated the role of...

journal_title：Molecular biology of the cell

authors： Chalat M,Moleschi K,Molday RS

更新日期：2017-02-01 00:00:00

abstract：:Polycystin (PC)1 and PC2 are membrane proteins implicated in autosomal dominant polycystic kidney disease. A physiologically relevant cleavage at PC1's G protein-coupled receptor proteolytic site (GPS) occurs early in the secretory pathway. Our results suggest that PC2 increases both PC1 GPS cleavage and PC1's appeara...

journal_title：Molecular biology of the cell

authors： Chapin HC,Rajendran V,Caplan MJ

更新日期：2010-12-01 00:00:00

abstract：:The mmd1 mutation causes temperature-sensitive growth and defects in mitochondrial morphology and distribution in the fission yeast Schizosaccharomyces pombe. In mutant cells, mitochondria aggregate at the two cell ends, with increased aggregation at elevated temperatures. Microtubules, which mediate mitochondrial pos...

journal_title：Molecular biology of the cell

authors： Weir BA,Yaffe MP

更新日期：2004-04-01 00:00:00

abstract：:Discoidin I expression was used as a marker to screen for mutants affected in the growth-differentiation transition (GDT) of Dictyostelium. By REMI mutagenesis we have isolated mutant 2-9, an overexpressor of discoidin I. It displays normal morphogenesis but shows premature entry into the developmental cycle. The disr...

journal_title：Molecular biology of the cell

authors： Zeng C,Anjard C,Riemann K,Konzok A,Nellen W

更新日期：2000-05-01 00:00:00

abstract：:The Golgi-associated retrograde protein (GARP) complex mediates tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). In the yeast Saccharomyces cerevisiae, GARP comprises four subunits named Vps51p, Vps52p, Vps53p, and Vps54p. Orthologues of the GARP subunits, except for Vps51p...

journal_title：Molecular biology of the cell

authors： Pérez-Victoria FJ,Schindler C,Magadán JG,Mardones GA,Delevoye C,Romao M,Raposo G,Bonifacino JS

更新日期：2010-10-01 00:00:00

abstract：:The activation of the cyclin-dependent kinase Cdk1 at the transition from interphase to mitosis induces important changes in microtubule dynamics. Cdk1 phosphorylates a number of microtubule- or tubulin-binding proteins but, hitherto, tubulin itself has not been detected as a Cdk1 substrate. Here we show that Cdk1 pho...

journal_title：Molecular biology of the cell

authors： Fourest-Lieuvin A,Peris L,Gache V,Garcia-Saez I,Juillan-Binard C,Lantez V,Job D

更新日期：2006-03-01 00:00:00

abstract：:A heterozygous Caveolin-1 c.474delA mutation has been identified in a family with heritable pulmonary arterial hypertension (PAH). This frameshift mutation leads to caveolin-1 protein that contains all known functional domains but has a change only in the final 20 amino acids of the C terminus. Here we studied how thi...

journal_title：Molecular biology of the cell

authors： Marsboom G,Chen Z,Yuan Y,Zhang Y,Tiruppathi C,Loyd JE,Austin ED,Machado RF,Minshall RD,Rehman J,Malik AB

更新日期：2017-03-15 00:00:00

abstract：:Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for epithelial polarity. A fraction of Stx3 is localized to late endosomes/lysosomes, although how it traffics there and its function in these organelles is unknown. Here we report that Stx3 underg...

journal_title：Molecular biology of the cell

authors： Giovannone AJ,Reales E,Bhattaram P,Fraile-Ramos A,Weimbs T

更新日期：2017-10-15 00:00:00

abstract：:Target of rapamycin complex 1 (TORC1) is a central cellular signaling coordinator that allows eukaryotic cells to adapt to the environment. In the budding yeast, Saccharomyces cerevisiae, TORC1 senses nitrogen and various stressors and modulates proteosynthesis, nitrogen uptake and metabolism, stress responses, and au...

journal_title：Molecular biology of the cell

authors： Takeda E,Jin N,Itakura E,Kira S,Kamada Y,Weisman LS,Noda T,Matsuura A

更新日期：2018-02-15 00:00:00

abstract：:Select lipid-anchored proteins such as glycosylphosphatidylinositol (GPI)-anchored proteins and nonreceptor tyrosine kinases may preferentially partition into sphingomyelin-rich and cholesterol-rich plasmalemmal microdomains, thereby acquiring resistance to detergent extraction. Two such domains, caveolae and lipid ra...

journal_title：Molecular biology of the cell

authors： Oh P,Schnitzer JE

更新日期：2001-03-01 00:00:00

abstract：:Brd2 is a member of the bromodomain extra terminal (BET) protein family, which consists of four chromatin-interacting proteins that regulate gene expression. Each BET protein contains two N-terminal bromodomains, which recognize acetylated histones, and the C-terminal protein-protein interaction domain. Using a genome...

journal_title：Molecular biology of the cell

authors： Hnilicová J,Hozeifi S,Stejskalová E,Dušková E,Poser I,Humpolíčková J,Hof M,Staněk D

更新日期：2013-11-01 00:00:00

abstract：:Significant increase in JNK, c-Jun, and Cdk5 activities are reported in Alzheimer's disease (AD). Inhibition of c-Jun prevents neuronal cell death in in vivo AD models, highlighting it as a major JNK effector. Both JNK and Cdk5 promote neurodegeneration upon deregulation; however, Cdk5 has not been mechanistically lin...

journal_title：Molecular biology of the cell

authors： Sun KH,Lee HG,Smith MA,Shah K

更新日期：2009-11-01 00:00:00

abstract：:A morphogenesis checkpoint in budding yeast delays nuclear division (and subsequent cell cycle progression) in cells that have failed to make a bud. We show that the ability of this checkpoint to delay nuclear division requires the SWE1 gene, encoding a protein kinase that inhibits the master cell cycle regulatory kin...

journal_title：Molecular biology of the cell

authors： Sia RA,Herald HA,Lew DJ

更新日期：1996-11-01 00:00:00

abstract：:The yeast [PSI+] prion is an epigenetic modifier of translation termination fidelity that causes nonsense suppression. The prion [PSI+] forms when the translation termination factor Sup35p adopts a self-propagating conformation. The presence of the [PSI+] prion modulates survivability in a variety of growth conditions...

journal_title：Molecular biology of the cell

authors： Strawn LA,Lin CA,Tank EM,Osman MM,Simpson SA,True HL

更新日期：2009-04-01 00:00:00

abstract：:EB1 proteins are ubiquitous microtubule-associated proteins involved in microtubule search and capture, regulation of microtubule dynamics, cell polarity, and chromosome stability. We have cloned a complete cDNA of Dictyostelium EB1 (DdEB1), the largest known EB1 homolog (57 kDa). Immunofluorescence analysis and expre...

journal_title：Molecular biology of the cell

authors： Rehberg M,Gräf R

更新日期：2002-07-01 00:00:00