Significance of 14-3-3 self-dimerization for phosphorylation-dependent target binding.

abstract：:Ion transport in various tissues can be regulated by the cortical actin cytoskeleton. Specifically, involvement of actin dynamics in the regulation of nonvoltage-gated sodium channels has been shown. Herein, inside-out patch clamp experiments were performed to study the effect of the heterodimeric actin capping protei...

journal_title：Molecular biology of the cell

authors： Shumilina EV,Negulyaev YA,Morachevskaya EA,Hinssen H,Khaitlina SY

更新日期：2003-04-01 00:00:00

abstract：:Rab 11 GTPase is an important regulator of endocytic membrane traffic. Recently, we and others have identified a novel family of Rab11 binding proteins, known as Rab11-family interacting proteins (FIPs). One of the family members, Rab coupling protein (RCP), was identified as a protein binding to both Rab4 and Rab11 G...

journal_title：Molecular biology of the cell

authors： Peden AA,Schonteich E,Chun J,Junutula JR,Scheller RH,Prekeris R

更新日期：2004-08-01 00:00:00

abstract：:Although evasion of apoptosis is thought to be required for the development of cancer, it is unclear which cell death pathways are evaded. We previously identified a novel epithelial cell death pathway that works in normal cells but is inactivated in tumor cells, implying that it may be targeted during tumor developme...

journal_title：Molecular biology of the cell

authors： Thorburn J,Moore F,Rao A,Barclay WW,Thomas LR,Grant KW,Cramer SD,Thorburn A

更新日期：2005-03-01 00:00:00

abstract：:The guanosine trisphosphatase Rap1 serves as a critical player in signal transduction, somatic cell proliferation and differentiation, and cell-cell adhesion by acting through distinct mechanisms. During mouse spermiogenesis, Rap1 is activated and forms a signaling complex with its effector, the serine-threonine kinas...

journal_title：Molecular biology of the cell

authors： Aivatiadou E,Mattei E,Ceriani M,Tilia L,Berruti G

更新日期：2007-04-01 00:00:00

abstract：:ARVCF, an armadillo-repeat protein of the p120(ctn) family, associates with classical cadherins and is present in adherens junctions, but its function is poorly understood. Here, we show that ARVCF interacts via a C-terminal PDZ-binding motif with zonula occludens (ZO)-1 and ZO-2. ARVCF and ZO-1 partially colocalize i...

journal_title：Molecular biology of the cell

authors： Kausalya PJ,Phua DC,Hunziker W

更新日期：2004-12-01 00:00:00

abstract：:The Rho GTPases RhoA and Rac1 function as master regulators of cytokinesis by controlling the actomyosin cytoskeleton. RhoA and Rac1 have to be respectively activated and inactivated at the division plane for cytokinesis to occur properly. The inactivation of Rac1 at the cleavage furrow is controlled by MgcRacGAP. How...

journal_title：Molecular biology of the cell

authors： Cannet A,Schmidt S,Delaval B,Debant A

更新日期：2014-12-15 00:00:00

abstract：:Phospholipase D (PLD) has been suggested to mediate epidermal growth factor (EGF) signaling. However, the molecular mechanism of EGF-induced PLD activation has not yet been elucidated. We investigated the importance of the phosphorylation and compartmentalization of PLD1 in EGF signaling. EGF treatment of COS-7 cells ...

journal_title：Molecular biology of the cell

authors： Han JM,Kim Y,Lee JS,Lee CS,Lee BD,Ohba M,Kuroki T,Suh PG,Ryu SH

更新日期：2002-11-01 00:00:00

abstract：:In the fission yeast Schizosaccharomyces pombe, centromeres remain clustered at the spindle-pole body (SPB) during mitotic interphase. In contrast, during meiotic prophase centromeres dissociate from the SPB. Here we examined the behavior of centromere proteins in living meiotic cells of S. pombe. We show that the Nuf...

journal_title：Molecular biology of the cell

authors： Asakawa H,Hayashi A,Haraguchi T,Hiraoka Y

更新日期：2005-05-01 00:00:00

abstract：:In this study, we examined the subcellular distribution and functions of LIMK1 in developing neurons. Confocal microscopy, subcellular fractionation, and expression of several epitope-tagged LIMK1 constructs revealed that LIMK1 is enriched in the Golgi apparatus and growth cones, with the LIM domain required for Golgi...

journal_title：Molecular biology of the cell

authors： Rosso S,Bollati F,Bisbal M,Peretti D,Sumi T,Nakamura T,Quiroga S,Ferreira A,Cáceres A

更新日期：2004-07-01 00:00:00

abstract：:Polalpha is the principal DNA polymerase for initiation of DNA replication and also functions in postinitiation DNA synthesis. In this study, we investigated the cell cycle responses induced by mutations in polalpha+. Germinating spores carrying either a deletion of polalpha+ (polalphaDelta) or a structurally intact b...

journal_title：Molecular biology of the cell

authors： Bhaumik D,Wang TS

更新日期：1998-08-01 00:00:00

abstract：:CD98, an early marker of T-cell activation, is an important regulator of integrin-mediated adhesion events. Previous studies suggest that CD98 is coupled to both cellular activation and transformation and is involved in the pathogenesis of viral infection, inflammatory disease, and cancer. Understanding of the molecul...

journal_title：Molecular biology of the cell

authors： Rintoul RC,Buttery RC,Mackinnon AC,Wong WS,Mosher D,Haslett C,Sethi T

更新日期：2002-08-01 00:00:00

abstract：:We have shown previously that interleukin-4 (IL-4) protects TS1alphabeta cells from apoptosis, but very little is known about the mechanism by which IL-4 exerts this effect. We found that Akt activity, which is dependent on phosphatidylinositol 3 kinase, is reduced in IL-4-deprived TS1alphabeta cells. Overexpression o...

journal_title：Molecular biology of the cell

authors： Cerezo A,Martínez-A C,Lanzarot D,Fischer S,Franke TF,Rebollo A

更新日期：1998-11-01 00:00:00

abstract：:Directed cell migration is mediated by cycles of protrusion, adhesion, traction generation on the extracellular matrix and retraction. However, how the events after protrusion are timed, and what dictates their temporal order is completely unknown. We used acute epidermal growth factor (EGF) stimulation of epidermal k...

journal_title：Molecular biology of the cell

authors： Schneider IC,Hays CK,Waterman CM

更新日期：2009-07-01 00:00:00

abstract：:Alternative pre-mRNA splicing patterns can change an extracellular stimulus, but the signaling pathways leading to these changes are still poorly characterized. Here, we describe a tyrosine-phosphorylated nuclear protein, YT521-B, and show that it interacts with the nuclear transcriptosomal component scaffold attachme...

journal_title：Molecular biology of the cell

authors： Hartmann AM,Nayler O,Schwaiger FW,Obermeier A,Stamm S

更新日期：1999-11-01 00:00:00

abstract：:Survivin, a subunit of the chromosome passenger complex (CPC), binds the N-terminal tail of histone H3, which is phosphorylated on T3 by Haspin kinase, and localizes the complex to the inner centromeres. We used x-ray crystallography to determine the residues of Survivin that are important in binding phosphomodified h...

journal_title：Molecular biology of the cell

authors： Niedzialkowska E,Wang F,Porebski PJ,Minor W,Higgins JM,Stukenberg PT

更新日期：2012-04-01 00:00:00

abstract：:Cystic fibrosis (CF) is a fatal genetic disorder associated with defective hydration of lung airways due to the loss of chloride transport through the CF transmembrane conductance regulator protein (CFTR). CFTR contains two membrane-spanning domains (MSDs), two nucleotide-binding domains (NBDs), and a regulatory domai...

journal_title：Molecular biology of the cell

authors： Ren HY,Grove DE,De La Rosa O,Houck SA,Sopha P,Van Goor F,Hoffman BJ,Cyr DM

更新日期：2013-10-01 00:00:00

abstract：:MCAK belongs to the Kinesin-13 family, whose members depolymerize microtubules rather than translocate along them. We defined the minimal functional unit of MCAK as the catalytic domain plus the class specific neck (MD-MCAK), which is consistent with previous reports. We used steady-state ATPase kinetics, microtubule ...

journal_title：Molecular biology of the cell

authors： Hertzer KM,Ems-McClung SC,Kline-Smith SL,Lipkin TG,Gilbert SP,Walczak CE

更新日期：2006-02-01 00:00:00

abstract：:Human neutrophils generally function adherent to an extracellular matrix. We have previously reported that upon adhesion to laminin- or fibronectin-coated, but not uncoated, plastic there is a depolymerization of actin in neutrophils. This phenomenon was not affected by inhibitors of the more well-studied components o...

journal_title：Molecular biology of the cell

authors： Wang JS,Coburn JP,Tauber AI,Zaner KS

更新日期：1997-01-01 00:00:00

abstract：:Microtubules (MTs) are often organized by a nucleus-associated MT organizing center (MTOC). In addition, in neurons and epithelial cells, motor-based transport of assembled MTs determines the polarity of the MT array. Here, we show that MT motility participates in MT organization in the fungus Ustilago maydis. In budd...

journal_title：Molecular biology of the cell

authors： Fink G,Steinberg G

更新日期：2006-07-01 00:00:00

abstract：:A major regulatory function has been evidenced here for HSF1, the key transcription factor of the heat-shock response, in a large-scale remodeling of the cell epigenome. Indeed, upon heat shock, HSF1, in addition to its well-known transactivating activities, mediates a genome-wide and massive histone deacetylation. In...

journal_title：Molecular biology of the cell

authors： Fritah S,Col E,Boyault C,Govin J,Sadoul K,Chiocca S,Christians E,Khochbin S,Jolly C,Vourc'h C

更新日期：2009-12-01 00:00:00

abstract：:The organization of the endoplasmic reticulum (ER) in the cortex of Xenopus oocytes was investigated during maturation and activation using a green fluorescent protein chimera, immunofluorescence, and electron microscopy. Dense clusters of ER developed on the vegetal side (the side opposite the meiotic spindle) during...

journal_title：Molecular biology of the cell

authors： Terasaki M,Runft LL,Hand AR

更新日期：2001-04-01 00:00:00

abstract：:We demonstrated that myosin IIA and IIB are essential for the formation of transverse arcs and ventral stress fibers, respectively. Furthermore, we illustrated the roles of both isoforms in lamellar flattening and also raised the possibility that actin filaments in ventral stress fibers are in a stretched conformation...

journal_title：Molecular biology of the cell

authors： Kuragano M,Uyeda TQP,Kamijo K,Murakami Y,Takahashi M

更新日期：2018-04-15 00:00:00

abstract：:Cancer cell migration requires the regulation of actin networks at protrusions associated with invadopodia and other leading edges. Carcinomas become invasive after undergoing an epithelial-mesenchymal transition characterized by the appearance of vimentin filaments. While vimentin expression correlates with cell migr...

journal_title：Molecular biology of the cell

authors： Lanier MH,Kim T,Cooper JA

更新日期：2015-12-15 00:00:00

abstract：:Human HT2-19 cells with a conditional cdk1 mutation stop dividing upon cdk1 inactivation and undergo multiple rounds of endoreplication. We show herein that major cell cycle events remain synchronized in these endoreplicating cells. DNA replication alternates with gap phases and cell cycle-specific cyclin E expression...

journal_title：Molecular biology of the cell

authors： Laronne A,Rotkopf S,Hellman A,Gruenbaum Y,Porter AC,Brandeis M

更新日期：2003-09-01 00:00:00

abstract：:Endoplasmic reticulum-localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum-associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from the ER lumen under conditions of ER stress. To elucidate the function of ERd...

journal_title：Molecular biology of the cell

authors： Fritz JM,Dong M,Apsley KS,Martin EP,Na CL,Sitaraman S,Weaver TE

更新日期：2014-02-01 00:00:00

abstract：:The Golgi-associated retrograde protein (GARP) complex mediates tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). In the yeast Saccharomyces cerevisiae, GARP comprises four subunits named Vps51p, Vps52p, Vps53p, and Vps54p. Orthologues of the GARP subunits, except for Vps51p...

journal_title：Molecular biology of the cell

authors： Pérez-Victoria FJ,Schindler C,Magadán JG,Mardones GA,Delevoye C,Romao M,Raposo G,Bonifacino JS

更新日期：2010-10-01 00:00:00

abstract：:E-cadherin plays an essential role in cell polarity and cell-cell adhesion; however, the pathway for delivery of E-cadherin to the basolateral membrane of epithelial cells has not been fully characterized. We first traced the post-Golgi, exocytic transport of GFP-tagged E-cadherin (Ecad-GFP) in unpolarized cells. In l...

journal_title：Molecular biology of the cell

authors： Lock JG,Stow JL

更新日期：2005-04-01 00:00:00

abstract：:GM3 synthase (SAT-I) is the primary glycosyltransferase responsible for the biosynthesis of ganglio-series gangliosides. In this study, we identify three isoforms of mouse SAT-I proteins, named M1-SAT-I, M2-SAT-I, and M3-SAT-I, which possess distinct lengths in their NH(2)-terminal cytoplasmic tails. These isoforms ar...

journal_title：Molecular biology of the cell

authors： Uemura S,Yoshida S,Shishido F,Inokuchi J

更新日期：2009-07-01 00:00:00

abstract：:In the phagocytic cell, NADPH oxidase (Nox2) system, cytoplasmic regulators (p47(phox), p67(phox), p40(phox), and Rac) translocate and associate with the membrane-spanning flavocytochrome b(558), leading to activation of superoxide production. We examined membrane targeting of phox proteins and explored conformational...

journal_title：Molecular biology of the cell

authors： Ueyama T,Tatsuno T,Kawasaki T,Tsujibe S,Shirai Y,Sumimoto H,Leto TL,Saito N

更新日期：2007-02-01 00:00:00

abstract：:Rab8 is a GTPase involved in membrane trafficking. In photoreceptor cells, rab8 is proposed to participate in the late stages of delivery of rhodopsin-containing post-Golgi membranes to the plasma membrane near the base of the connecting cilium. To test the function of rab8 in vivo, we generated transgenic Xenopus lae...

journal_title：Molecular biology of the cell

authors： Moritz OL,Tam BM,Hurd LL,Peränen J,Deretic D,Papermaster DS

更新日期：2001-08-01 00:00:00