Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components.

abstract：:DNA synthesis-coupled proteolysis of the prereplicative complex component Cdt1 by the CRL4(Cdt2) E3 ubiquitin ligase is thought to help prevent rereplication of the genome during S phase. To directly test whether CRL4(Cdt2)-triggered destruction of Cdt1 is required for normal cell cycle progression in vivo, we express...

journal_title：Molecular biology of the cell

authors： Lee HO,Zacharek SJ,Xiong Y,Duronio RJ

更新日期：2010-11-01 00:00:00

abstract：:The gene encoding NFKB1 is autoregulated, responding to NF-kappa B/Rel activation through NF-kappa B binding sites in its promoter, which also contains putative sites for Ets proteins. One of the Ets sites, which we refer to as EBS4, is located next to an NF-kappa B/Rel binding site, kB3, which is absolutely required ...

journal_title：Molecular biology of the cell

authors： Lambert PF,Ludford-Menting MJ,Deacon NJ,Kola I,Doherty RR

更新日期：1997-02-01 00:00:00

abstract：:ELMO2 belongs to a family of scaffold proteins involved in phagocytosis and cell motility. ELMO2 can simultaneously bind integrin-linked kinase (ILK) and RhoG, forming tripartite ERI complexes. These complexes are involved in promoting β1 integrin-dependent directional migration in undifferentiated epidermal keratinoc...

journal_title：Molecular biology of the cell

authors： Jackson BC,Ivanova IA,Dagnino L

更新日期：2015-07-15 00:00:00

abstract：:Cell-cell fusion is an intriguing differentiation process, essential for placental development and maturation. A proteomic approach identified a cytoplasmic protein, calponin 3 (CNN3), related to the fusion of BeWo choriocarcinoma cells. CNN3 was expressed in cytotrophoblasts in human placenta. CNN3 gene knockdown pro...

journal_title：Molecular biology of the cell

authors： Shibukawa Y,Yamazaki N,Kumasawa K,Daimon E,Tajiri M,Okada Y,Ikawa M,Wada Y

更新日期：2010-11-15 00:00:00

abstract：:We report a new nuclear gene, designated ATP25 (reading frame YMR098C on chromosome XIII), required for expression of Atp9p (subunit 9) of the Saccharomyces cerevisiae mitochondrial proton translocating ATPase. Mutations in ATP25 elicit a deficit of ATP9 mRNA and of its translation product, thereby preventing assembly...

journal_title：Molecular biology of the cell

authors： Zeng X,Barros MH,Shulman T,Tzagoloff A

更新日期：2008-04-01 00:00:00

abstract：:Yeast that naturally exhaust the glucose from their environment differentiate into three distinct cell types distinguishable by flow cytometry. Among these is a quiescent (Q) population, which is so named because of its uniform but readily reversed G1 arrest, its fortified cell walls, heat tolerance, and longevity. Da...

journal_title：Molecular biology of the cell

authors： Li L,Miles S,Melville Z,Prasad A,Bradley G,Breeden LL

更新日期：2013-12-01 00:00:00

abstract：:A well-established function of centrosomes is their role in accomplishing a successful mitosis that gives rise to a pair of identical daughter cells. We recently showed that DNA replication defects and DNA damage in Drosophila embryos trigger centrosomal changes, but it remained unclear whether comparable centrosomal ...

journal_title：Molecular biology of the cell

authors： Hut HM,Lemstra W,Blaauw EH,Van Cappellen GW,Kampinga HH,Sibon OC

更新日期：2003-05-01 00:00:00

abstract：:Intracellular trafficking of tRNA was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. This view has recently been challenged, however, by the discovery that tRNA can move retrograde from the cytoplasm back to the nucleus in Saccharomyces cerev...

journal_title：Molecular biology of the cell

authors： Chafe SC,Pierce JB,Eswara MB,McGuire AT,Mangroo D

更新日期：2011-04-01 00:00:00

abstract：:The guanosine trisphosphatase Rap1 serves as a critical player in signal transduction, somatic cell proliferation and differentiation, and cell-cell adhesion by acting through distinct mechanisms. During mouse spermiogenesis, Rap1 is activated and forms a signaling complex with its effector, the serine-threonine kinas...

journal_title：Molecular biology of the cell

authors： Aivatiadou E,Mattei E,Ceriani M,Tilia L,Berruti G

更新日期：2007-04-01 00:00:00

abstract：:The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms in which 30-nm fibers have ...

journal_title：Molecular biology of the cell

authors： Cai S,Song Y,Chen C,Shi J,Gan L

更新日期：2018-07-01 00:00:00

abstract：:"Lipid raft" is the name given to the tiny, dynamic, and ordered domains of cholesterol and sphingolipids that are hypothesized to exist in the plasma membranes of eukaryotic cells. According to the lipid raft hypothesis, these cholesterol- and sphingolipid-enriched domains modulate the protein-protein interactions th...

journal_title：Molecular biology of the cell

authors： Kraft ML

更新日期：2013-09-01 00:00:00

abstract：:Rho family proteins have been implicated in regulating various cellular processes, including actin cytoskeleton organization, endocytosis, cell cycle, and gene expression. In this study, we analyzed the function of a novel Dictyostelium discoideum Rho family protein (RacC). A cell line was generated that conditionally...

journal_title：Molecular biology of the cell

authors： Seastone DJ,Lee E,Bush J,Knecht D,Cardelli J

更新日期：1998-10-01 00:00:00

abstract：:Sec13p has been thought to be an essential component of the COPII coat, required for exit of proteins from the yeast endoplasmic reticulum (ER). We show herein that normal function of Sec13p was not required for ER exit of the Hsp150 glycoprotein. Hsp150 was secreted to the medium under restrictive conditions in a sec...

journal_title：Molecular biology of the cell

authors： Fatal N,Suntio T,Makarow M

更新日期：2002-12-01 00:00:00

abstract：:Membrane tubules of uniform diameter (60-80 nm) and variable lengths have been seen to extend from the main bodies of the Golgi complex, trans Golgi network (TGN), and endosomes. In the case of endosomes, these tubules appear to mediate membrane and receptor recycling events. Brefeldin A (BFA) is a potent drug that co...

journal_title：Molecular biology of the cell

authors： de Figueiredo P,Brown WJ

更新日期：1995-07-01 00:00:00

abstract：:Nuclear FGFR1 acts as a developmental gene regulator in cooperation with FGF-2, RSK1, and CREB-binding protein (CBP). FRAP analysis revealed three nuclear FGFR1 populations: i) a fast mobile, ii) a slower mobile population reflecting chromatin-bound FGFR1, and iii) an immobile FGFR1 population associated with the nucl...

journal_title：Molecular biology of the cell

authors： Dunham-Ems SM,Lee YW,Stachowiak EK,Pudavar H,Claus P,Prasad PN,Stachowiak MK

更新日期：2009-05-01 00:00:00

abstract：:Yeast TGN resident proteins that frequently cycle between the TGN and endosomes are much more slowly transported to the prevacuolar/late endosomal compartment (PVC) than other proteins. However, TGN protein transport to the PVC is accelerated in mutants lacking function of Inp53p. Inp53p contains a SacI polyphosphoino...

journal_title：Molecular biology of the cell

authors： Ha SA,Torabinejad J,DeWald DB,Wenk MR,Lucast L,De Camilli P,Newitt RA,Aebersold R,Nothwehr SF

更新日期：2003-04-01 00:00:00

abstract：:Differentiation and polarization of epithelial cells depends on the formation of the apical junctional complex (AJC), which is composed of the tight junction (TJ) and the adherens junction (AJ). In this study, we investigated mechanisms of actin reorganization that drive the establishment of AJC. Using a calcium switc...

journal_title：Molecular biology of the cell

authors： Ivanov AI,Hunt D,Utech M,Nusrat A,Parkos CA

更新日期：2005-06-01 00:00:00

abstract：:Quite a few regulatory proteins, including transcription factors, are normally maintained in a dormant state to be activated after internal or environmental cues. Recently, a novel strategy, requiring proteolytic cleavage, was described for the mobilization of dormant transcription factors. These transcription factors...

journal_title：Molecular biology of the cell

authors： Becker-Herman S,Arie G,Medvedovsky H,Kerem A,Shachar I

更新日期：2005-11-01 00:00:00

abstract：:B-type cyclins are rapidly degraded at the transition between metaphase and anaphase and their ubiquitin-mediated proteolysis is required for cells to exit mitosis. We used a novel enrichment to isolate new budding mutants that arrest the cell cycle in mitosis. Most of these mutants lie in the CDC16, CDC23, and CDC27 ...

journal_title：Molecular biology of the cell

authors： Hwang LH,Murray AW

更新日期：1997-10-01 00:00:00

abstract：:The Cdc42 guanosine triphosphatase (GTPase) plays a central role in polarity development in species ranging from yeast to humans. In budding yeast, a specific growth site is selected in the G1 phase. Rsr1, a Ras GTPase, interacts with Cdc42 and its associated proteins to promote polarized growth at the proper bud site...

journal_title：Molecular biology of the cell

authors： Miller KE,Lo WC,Chou CS,Park HO

更新日期：2019-09-15 00:00:00

abstract：:Functionally different subsets of actin filament arrays contribute to cellular organization and motility. We report the identification of a novel subset of loose actin filament arrays through regulated association with the widely expressed protein SWAP-70. These loose actin filament arrays were commonly located behind...

journal_title：Molecular biology of the cell

authors： Hilpelä P,Oberbanscheidt P,Hahne P,Hund M,Kalhammer G,Small JV,Bähler M

更新日期：2003-08-01 00:00:00

abstract：:Targeting of peroxisomal membrane proteins (PMPs) is a multistep process that requires not only recognition of PMPs in the cytosol but also their insertion into the peroxisomal membrane. As a consequence, targeting signals of PMPs (mPTS) are rather complex. A candidate protein for the PMP recognition event is Pex19p, ...

journal_title：Molecular biology of the cell

authors： Rottensteiner H,Kramer A,Lorenzen S,Stein K,Landgraf C,Volkmer-Engert R,Erdmann R

更新日期：2004-07-01 00:00:00

abstract：:The budding yeast formins Bni1 and Bnr1 control the assembly of actin cables. These formins exhibit distinct patterns of localization and polymerize two different populations of cables: Bni1 in the bud and Bnr1 in the mother cell. We generated a functional Bni1-3GFP that improved the visualization of Bni1 in vivo at e...

journal_title：Molecular biology of the cell

authors： Buttery SM,Yoshida S,Pellman D

更新日期：2007-05-01 00:00:00

abstract：:Chromatin and nuclear pore complexes (NPCs) undergo dramatic changes during mitosis, which in vertebrates and Aspergillus nidulans involves movement of Nup2 from NPCs to the chromatin region to fulfill unknown functions. This transition is shown to require the Cdk1 mitotic kinase and be promoted prematurely by ectopic...

journal_title：Molecular biology of the cell

authors： Markossian S,Suresh S,Osmani AH,Osmani SA

更新日期：2015-02-15 00:00:00

abstract：:Cilia and flagella are structurally and functionally conserved organelles present in basal as well as higher eukaryotes. The assembly of cilia requires a microtubule based scaffold called a basal body. The ninefold symmetry characteristic of basal bodies and the structurally similar centriole is organized around a hub...

journal_title：Molecular biology of the cell

authors： Culver BP,Meehl JB,Giddings TH Jr,Winey M

更新日期：2009-03-01 00:00:00

abstract：:Although the distribution of the cation-independent mannose 6-phosphate receptor (CI-MPR) has been well studied, its intracellular itinerary and trafficking kinetics remain uncertain. In this report, we describe the endocytic trafficking and steady-state localization of a chimeric form of the CI-MPR containing the ect...

journal_title：Molecular biology of the cell

authors： Lin SX,Mallet WG,Huang AY,Maxfield FR

更新日期：2004-02-01 00:00:00

abstract：:Lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) and their counterreceptors such as intercellular cell adhesion molecules (ICAM-1 and ICAM-2), junctional adhesion molecules (JAM-A, JAM-C), and receptors for advanced glycation end products (RAGE) are crucial for promoting polymorphonucl...

journal_title：Molecular biology of the cell

authors： Li N,Yang H,Wang M,Lü S,Zhang Y,Long M

更新日期：2018-02-15 00:00:00

abstract：:Breast epithelial cells cultured in three-dimensional (3D) collagen gels undergo ductal morphogenesis when the gel is compliant and they can achieve tensional homeostasis. We previously showed that this process requires down-regulation of Rho in compliant collagen gels, but the mechanism remains undefined. In this stu...

journal_title：Molecular biology of the cell

authors： Ponik SM,Trier SM,Wozniak MA,Eliceiri KW,Keely PJ

更新日期：2013-06-01 00:00:00

abstract：:Signaling via the Ras pathway involves sequential activation of Ras, Raf-1, mitogen-activated protein kinase kinase (MKK), and the extracellular signal-regulated (ERK) group of mitogen-activated protein (MAP) kinases. Expression from the c-Fos, atrial natriuretic factor (ANF), and myosin light chain-2 (MLC-2) promoter...

journal_title：Molecular biology of the cell

authors： Thorburn J,Carlson M,Mansour SJ,Chien KR,Ahn NG,Thorburn A

更新日期：1995-11-01 00:00:00

abstract：:The reovirus fusion-associated small transmembrane (FAST) proteins comprise a unique family of viral membrane fusion proteins dedicated to inducing cell-cell fusion. We recently reported that a polybasic motif (PBM) in the cytosolic tail of reptilian reovirus p14 FAST protein functions as a novel tribasic Golgi export...

journal_title：Molecular biology of the cell

authors： Parmar HB,Duncan R

更新日期：2016-04-15 00:00:00