Overexpression of a novel rho family GTPase, RacC, induces unusual actin-based structures and positively affects phagocytosis in Dictyostelium discoideum.

abstract：:The role of plasma membrane (PM) area as a critical factor during cell motility is poorly understood, mainly due to an inability to precisely follow PM area dynamics. To address this fundamental question, we developed static and dynamic assays to follow exocytosis, endocytosis, and PM area changes during fibroblast sp...

journal_title：Molecular biology of the cell

authors： Gauthier NC,Rossier OM,Mathur A,Hone JC,Sheetz MP

更新日期：2009-07-01 00:00:00

abstract：:Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin-8s on microtubule dynamics, it remains unclear whether the kinesin-8 mitotic phenotypes are consequence...

journal_title：Molecular biology of the cell

authors： Gergely ZR,Crapo A,Hough LE,McIntosh JR,Betterton MD

更新日期：2016-11-07 00:00:00

abstract：:Eukaryotic cell survival requires maintenance of plasma membrane (PM) homeostasis in response to environmental insults and changes in lipid metabolism. In yeast, a key regulator of PM homeostasis is target of rapamycin (TOR) complex 2 (TORC2), a multiprotein complex containing the evolutionarily conserved TOR protein ...

journal_title：Molecular biology of the cell

authors： Martinez Marshall MN,Emmerstorfer-Augustin A,Leskoske KL,Zhang LH,Li B,Thorner J

更新日期：2019-06-01 00:00:00

abstract：:The role of platelet endothelial cell adhesion molecule-1 (PECAM-1) in endothelial cell-cell interactions and its contribution to cadherin-mediated cell adhesion are poorly understood. Such studies have been difficult because all known endothelial cells express PECAM-1. We have used Madin-Darby canine kidney (MDCK) ce...

journal_title：Molecular biology of the cell

authors： Sheibani N,Sorenson CM,Frazier WA

更新日期：2000-08-01 00:00:00

abstract：:The orderly progression through the cell division cycle is of paramount importance to all organisms, as improper progression through the cycle could result in defects with grave consequences. Previously, our lab has shown that model eukaryotes such as Saccharomyces cerevisiae, Caenorhabditis elegans, and Danio rerio a...

journal_title：Molecular biology of the cell

authors： Chan K,Goldmark JP,Roth MB

更新日期：2010-07-01 00:00:00

abstract：:Transforming growth factor-beta1 (TGF-beta) can be tumor suppressive, but it can also enhance tumor progression by stimulating the complex process of epithelial-to-mesenchymal transdifferentiaion (EMT). The signaling pathway(s) that regulate EMT in response to TGF-beta are not well understood. We demonstrate the acqui...

journal_title：Molecular biology of the cell

authors： Bhowmick NA,Ghiassi M,Bakin A,Aakre M,Lundquist CA,Engel ME,Arteaga CL,Moses HL

更新日期：2001-01-01 00:00:00

abstract：:Mechanical cues regulate the function of a broad range of stem cells in culture and in tissue. For example, soft substrates promote the neuronal differentiation of neural stem cells (NSCs) by suppressing cytoskeletal contractility. However, the mechanisms that link cytoskeletal signaling to the transcriptional regulat...

journal_title：Molecular biology of the cell

authors： Kang PH,Schaffer DV,Kumar S

更新日期：2020-03-01 00:00:00

abstract：:SNAP-23 is a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) involved in Fc receptor (FcR)-mediated phagocytosis. However, the regulatory mechanism underlying its function remains elusive. Using phosphorylation-specific antibodies, SNAP-23 was found to be phosph...

journal_title：Molecular biology of the cell

authors： Sakurai C,Itakura M,Kinoshita D,Arai S,Hashimoto H,Wada I,Hatsuzawa K

更新日期：2018-07-15 00:00:00

abstract：:Previously, we showed that spliceosomal U6 small nuclear RNA (snRNA) transiently passes through the nucleolus. Herein, we report that all individual snRNAs of the [U4/U6.U5] tri-snRNP localize to nucleoli, demonstrated by fluorescence microscopy of nucleolar preparations after injection of fluorescein-labeled snRNA in...

journal_title：Molecular biology of the cell

authors： Gerbi SA,Lange TS

更新日期：2002-09-01 00:00:00

abstract：:Desmin interacts with nebulin establishing a direct link between the intermediate filament network and sarcomeres at the Z-discs. Here, we examined a desmin mutation, E245D, that is located within the coil IB (nebulin-binding) region of desmin and that has been reported to cause human cardiomyopathy and skeletal muscl...

journal_title：Molecular biology of the cell

authors： Conover GM,Henderson SN,Gregorio CC

更新日期：2009-02-01 00:00:00

abstract：:Tumor necrosis factor α (TNF-α) receptor-associated factor 2 (TRAF2) regulates activation of the c-Jun N-terminal kinase (JNK)/c-Jun and the inhibitor of κB kinase (IKK)/nuclear factor κB (NF-κB) signaling cascades in response to TNF-α stimulation. Gene knockout studies have revealed that TRAF2 inhibits TNF-α-induced ...

journal_title：Molecular biology of the cell

authors： Zhang L,Blackwell K,Altaeva A,Shi Z,Habelhah H

更新日期：2011-01-01 00:00:00

abstract：:Insulin regulates glucose uptake into fat and muscle by modulating the distribution of the GLUT4 glucose transporter between the surface and interior of cells. The GLUT4 trafficking pathway overlaps with the general endocytic recycling pathway, but the degree and functional significance of the overlap are not known. I...

journal_title：Molecular biology of the cell

authors： Zeigerer A,Lampson MA,Karylowski O,Sabatini DD,Adesnik M,Ren M,McGraw TE

更新日期：2002-07-01 00:00:00

abstract：:Cholesterol is an essential constituent of membranes in mammalian cells. The plasma membrane and the endocytic recycling compartment (ERC) are both highly enriched in cholesterol. The abundance and distribution of cholesterol among organelles are tightly controlled by a combination of mechanisms involving vesicular an...

journal_title：Molecular biology of the cell

authors： Iaea DB,Mao S,Lund FW,Maxfield FR

更新日期：2017-04-15 00:00:00

abstract：:Cholesterol regulates plasma membrane (PM) association and functioning of syntaxin-4 and soluble N-ethylmaleimide-sensitive fusion protein 23 (SNAP23) in the secretory pathway. However, the molecular mechanism and cellular cholesterol pools that determine the localization and assembly of these target membrane SNAP rec...

journal_title：Molecular biology of the cell

authors： Reverter M,Rentero C,de Muga SV,Alvarez-Guaita A,Mulay V,Cairns R,Wood P,Monastyrskaya K,Pol A,Tebar F,Blasi J,Grewal T,Enrich C

更新日期：2011-11-01 00:00:00

abstract：:CRM1 (Exportin1/XPO1) exports hundreds of broadly functioning protein cargoes out of the cell nucleus by binding to their classical nuclear export signals (NESs). The 8- to 15-amino-acid-long NESs contain four to five hydrophobic residues and are highly diverse in both sequence and CRM1-bound structure. Here we examin...

journal_title：Molecular biology of the cell

authors： Fu SC,Fung HYJ,Cağatay T,Baumhardt J,Chook YM

更新日期：2018-08-15 00:00:00

abstract：:Activation of the c-myc proto-oncogene by chromosomal translocation or proviral insertion frequently results in the separation of the c-myc coding region from its normal regulatory elements. Such rearrangements are often accompanied by loss or mutation of c-myc exon 1 sequences. These genetic alterations do not affect...

journal_title：Molecular biology of the cell

authors： Blackwood EM,Lugo TG,Kretzner L,King MW,Street AJ,Witte ON,Eisenman RN

更新日期：1994-05-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the class C vacuole protein sorting (Vps) proteins, together with Vam2p/Vps41p and Vam6p/Vps39p, form a complex that interacts with soluble N-ethylmaleimide-sensitive factor attachment protein receptor and Rab proteins to "tether" vacuolar membranes before fusion. To determine a role for t...

journal_title：Molecular biology of the cell

authors： Richardson SC,Winistorfer SC,Poupon V,Luzio JP,Piper RC

更新日期：2004-03-01 00:00:00

abstract：:Abundant evidence has shown that the GTPase dynamin is required for receptor-mediated endocytosis, but its exact role in endocytic clathrin-coated vesicle formation remains to be established. Whereas dynamin GTPase domain mutants that are defective in GTP binding and hydrolysis are potent dominant-negative inhibitors ...

journal_title：Molecular biology of the cell

authors： Damke H,Binns DD,Ueda H,Schmid SL,Baba T

更新日期：2001-09-01 00:00:00

abstract：:Dynamic regulation of cytoskeletal contractility through phosphorylation of the nonmuscle Myosin-II regulatory light chain (MRLC) provides an essential source of tension for shaping epithelial tissues. Rho GTPase and its effector kinase ROCK have been implicated in regulating MRLC phosphorylation in vivo, but evidence...

journal_title：Molecular biology of the cell

authors： Neubueser D,Hipfner DR

更新日期：2010-08-15 00:00:00

abstract：:In mammals, repression of translation during stress is associated with the assembly of stress granules in the cytoplasm, which contain a fraction of arrested mRNA and have been proposed to play a role in their storage. Because physical contacts are seen with GW bodies, which contain the mRNA degradation machinery, str...

journal_title：Molecular biology of the cell

authors： Mollet S,Cougot N,Wilczynska A,Dautry F,Kress M,Bertrand E,Weil D

更新日期：2008-10-01 00:00:00

abstract：:Several membrane-associating signals, including covalently linked fatty acids, are found in various combinations at the N termini of signaling proteins. The function of these combinations was investigated by appending fatty acylated N-terminal sequences to green fluorescent protein (GFP). Myristoylated plus mono/dipal...

journal_title：Molecular biology of the cell

authors： McCabe JB,Berthiaume LG

更新日期：1999-11-01 00:00:00

abstract：:When demembranated sperm nuclei are placed in a Xenopus egg extract, they become surrounded by a nuclear envelope and then swell to form morphologically typical pronuclei. Granules ranging from < 1.0 to approximately 3.0 microns in diameter appear within such nuclei. Bell et al. identified four nucleolar proteins in t...

journal_title：Molecular biology of the cell

authors： Bauer DW,Murphy C,Wu Z,Wu CH,Gall JG

更新日期：1994-06-01 00:00:00

abstract：:Cytokinesis requires the coordination of many cellular complexes, particularly those involved in the constriction and reconstruction of the plasma membrane in the cleavage furrow. We have investigated the regulation and function of vesicle transport and fusion during cytokinesis in budding yeast. By using time-lapse c...

journal_title：Molecular biology of the cell

authors： VerPlank L,Li R

更新日期：2005-05-01 00:00:00

abstract：:The gamma-tubulin ring complex (gammaTuRC), consisting of multiple protein subunits, can nucleate microtubule assembly. Although many subunits of the gammaTuRC have been identified, a complete set remains to be defined in any organism. In addition, how the subunits interact with each other to assemble into gammaTuRC r...

journal_title：Molecular biology of the cell

authors： Gunawardane RN,Martin OC,Zheng Y

更新日期：2003-03-01 00:00:00

abstract：:The scaffolding protein anillin is required for completion of cytokinesis. Anillin binds filamentous (F) actin, nonmuscle myosin II, and septins and in cell culture models has been shown to restrict actomyosin contractility to the cleavage furrow. Whether anillin also serves this function during the incomplete cytokin...

journal_title：Molecular biology of the cell

authors： Goldbach P,Wong R,Beise N,Sarpal R,Trimble WS,Brill JA

更新日期：2010-05-01 00:00:00

abstract：:Large cargoes require multiple receptors for efficient transport through the nuclear pore complex. The 60S ribosomal subunit is one of the bulkiest transport cargoes, and in yeast three different receptors, Crm1, Mex67/Mtr2, and Arx1, collaborate in its export. However, only Crm1, recruited by the adapter Nmd3, appear...

journal_title：Molecular biology of the cell

authors： Lo KY,Johnson AW

更新日期：2009-03-01 00:00:00

abstract：:During stem cell divisions, mitotic microtubules do more than just segregate the chromosomes. They also determine whether a cell divides virtually symmetrically or asymmetrically by establishing spindle orientation and the plane of cell division. This can be decisive for the fate of the stem cell progeny. Spindle defe...

journal_title：Molecular biology of the cell

authors： Mora-Bermúdez F,Huttner WB

更新日期：2015-12-01 00:00:00

abstract：:Two pore-domain potassium (K2P) channels mediate potassium background currents that stabilize the resting membrane potential and facilitate action potential repolarization. In the human heart, hK2P17.1 channels are predominantly expressed in the atria and Purkinje cells. Reduced atrial hK2P17.1 protein levels were des...

journal_title：Molecular biology of the cell

authors： Wiedmann F,Schlund D,Voigt N,Ratte A,Kraft M,Katus HA,Schmidt C

更新日期：2019-06-01 00:00:00

abstract：:Two different human diseases, X-linked myotubular myopathy and Charcot-Marie-Tooth disease, result from mutant MTM1 or MTMR2 lipid phosphatases. Although events involved in endosomal PI(3)P and PI(3,5)P(2) synthesis are well established and pivotal in receptor signaling and degradation, enzymes involved in phosphoinos...

journal_title：Molecular biology of the cell

authors： Cao C,Backer JM,Laporte J,Bedrick EJ,Wandinger-Ness A

更新日期：2008-08-01 00:00:00

abstract：:The yeast [PSI+] prion is an epigenetic modifier of translation termination fidelity that causes nonsense suppression. The prion [PSI+] forms when the translation termination factor Sup35p adopts a self-propagating conformation. The presence of the [PSI+] prion modulates survivability in a variety of growth conditions...

journal_title：Molecular biology of the cell

authors： Strawn LA,Lin CA,Tank EM,Osman MM,Simpson SA,True HL

更新日期：2009-04-01 00:00:00