Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling.

abstract：:Endoplasmic reticulum-localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum-associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from the ER lumen under conditions of ER stress. To elucidate the function of ERd...

journal_title：Molecular biology of the cell

authors： Fritz JM,Dong M,Apsley KS,Martin EP,Na CL,Sitaraman S,Weaver TE

更新日期：2014-02-01 00:00:00

abstract：:Activation of the high-osmolarity glycerol (HOG) pathway for osmoregulation in the yeast Saccharomyces cerevisiae involves interaction of the adaptor Ste50p with the cytoplasmic tail of single-transmembrane protein Opy2p. We have determined the solution structure of the Ste50p-RA (Ras association) domain, and it shows...

journal_title：Molecular biology of the cell

authors： Ekiel I,Sulea T,Jansen G,Kowalik M,Minailiuc O,Cheng J,Harcus D,Cygler M,Whiteway M,Wu C

更新日期：2009-12-01 00:00:00

abstract：:We conducted a genetic screen for mutations in myospheroid, the gene encoding the Drosophila betaPS integrin subunit, and identified point mutants in all of the structural domains of the protein. Surprisingly, we find that mutations in very strongly conserved residues will often allow sufficient integrin function to s...

journal_title：Molecular biology of the cell

authors： Jannuzi AL,Bunch TA,West RF,Brower DL

更新日期：2004-08-01 00:00:00

abstract：:We have isolated mutants in the fission yeast Schizosaccharomyces pombe that are defective in protein glycosylation. A collection of osmotically sensitive mutants was prepared and screened for glycosylation defects using lectin staining as an assay. Mutants singly defective in four glycoprotein synthesis genes (gps1-4...

journal_title：Molecular biology of the cell

authors： Huang KM,Snider MD

更新日期：1995-05-01 00:00:00

abstract：:Cytokinesis in metazoan cells requires a set of antiparallel microtubules that become bundled upon anaphase onset to form a structure known as the central spindle. Bundling of these microtubules requires a protein complex, centralspindlin, that consists of the CYK-4/MgcRacGAP Rho-family GTPase-activating protein and t...

journal_title：Molecular biology of the cell

authors： Pavicic-Kaltenbrunner V,Mishima M,Glotzer M

更新日期：2007-12-01 00:00:00

abstract：:Mechanically stressed cells display increased levels of fos message and protein. Although the intracellular signaling pathways responsible for FOS induction have been extensively characterized, we still do not understand the nature of the primary cell mechanotransduction event responsible for converting an externally ...

journal_title：Molecular biology of the cell

authors： Grembowicz KP,Sprague D,McNeil PL

更新日期：1999-04-01 00:00:00

abstract：:Synaptic vesicles (SV) are generated by two different mechanisms, one AP-2 dependent and one AP-3 dependent. It has been uncertain, however, whether these mechanisms generate SV that differ in molecular composition. We explored this hypothesis by analyzing the targeting of ZnT3 and synaptophysin both to PC12 synaptic-...

journal_title：Molecular biology of the cell

authors： Salazar G,Love R,Werner E,Doucette MM,Cheng S,Levey A,Faundez V

更新日期：2004-02-01 00:00:00

abstract：:Dopamine (DA) increases Na(+),K(+)-ATPase activity in lung alveolar epithelial cells. This effect is associated with an increase in Na(+),K(+)-ATPase molecules within the plasma membrane (). Analysis of Na(+),K(+)-ATPase motion was performed in real-time in alveolar cells stably expressing Na(+),K(+)-ATPase molecules ...

journal_title：Molecular biology of the cell

authors： Bertorello AM,Komarova Y,Smith K,Leibiger IB,Efendiev R,Pedemonte CH,Borisy G,Sznajder JI

更新日期：2003-03-01 00:00:00

abstract：:Trimming of N-linked oligosaccharides by endoplasmic reticulum (ER) glucosidase II is implicated in quality control of protein folding. An alternate glucosidase II-independent deglucosylation pathway exists, in which endo-alpha-mannosidase cleaves internally the glucose-substituted mannose residue of oligosaccharides....

journal_title：Molecular biology of the cell

authors： Zuber C,Spiro MJ,Guhl B,Spiro RG,Roth J

更新日期：2000-12-01 00:00:00

abstract：:Polypeptides targeted to the yeast endoplasmic reticulum (ER) posttranslationally are thought to be kept in the cytoplasm in an unfolded state by Hsp70 chaperones before translocation. We show here that Escherichia coli beta-lactamase associated with Hsp70, but adopted a native-like conformation before translocation i...

journal_title：Molecular biology of the cell

authors： Paunola E,Suntio T,Jämsä E,Makarow M

更新日期：1998-04-01 00:00:00

abstract：:Tubulogenesis by epithelial cells regulates kidney, lung, and mammary development, whereas that by endothelial cells regulates vascular development. Although functionally dissimilar, the processes necessary for tubulation by epithelial and endothelial cells are very similar. We performed microarray analysis to further...

journal_title：Molecular biology of the cell

authors： Albig AR,Schiemann WP

更新日期：2005-02-01 00:00:00

abstract：:A cell surface proteoglycan, syndecan-1, has been shown to participate in the maintenance of the epithelial cell morphology. A point mutated activated c-Ha-ras gene under the control of the glucocorticoid inducible MMTV-LTR promoter was transfected into the mouse mammary epithelial cell line, NOG-8. The NOG-8 ras cell...

journal_title：Molecular biology of the cell

authors： Kirjavainen J,Leppä S,Hynes NE,Jalkanen M

更新日期：1993-08-01 00:00:00

abstract：:Microtubule-associated protein 1B (MAP1B) is essential for neural development. Besides the abundant expression in neurons, MAP1B recently was found in myelinating oligodendroglia. Moreover, MAP1B deficiency causes delayed myelin development, suggesting the functional importance of MAP1B in oligodendroglia. However, mo...

journal_title：Molecular biology of the cell

authors： Zhao L,Ku L,Chen Y,Xia M,LoPresti P,Feng Y

更新日期：2006-10-01 00:00:00

abstract：:A heterozygous Caveolin-1 c.474delA mutation has been identified in a family with heritable pulmonary arterial hypertension (PAH). This frameshift mutation leads to caveolin-1 protein that contains all known functional domains but has a change only in the final 20 amino acids of the C terminus. Here we studied how thi...

journal_title：Molecular biology of the cell

authors： Marsboom G,Chen Z,Yuan Y,Zhang Y,Tiruppathi C,Loyd JE,Austin ED,Machado RF,Minshall RD,Rehman J,Malik AB

更新日期：2017-03-15 00:00:00

abstract：:The mannose 6-phosphate (Man-6-P) lysosomal targeting signal on acid hydrolases is synthesized by the sequential action of uridine 5'-diphosphate-N-acetylglucosamine: lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase) and GlcNAc-1-phosphodiester alpha-N-acetylglucosaminidase ("unco...

journal_title：Molecular biology of the cell

authors： Boonen M,Vogel P,Platt KA,Dahms N,Kornfeld S

更新日期：2009-10-01 00:00:00

abstract：:Cell-to-extracellular matrix adhesion is regulated by a multitude of pathways initiated distally to the core cell-matrix adhesion machinery, such as via growth factor signaling. In contrast to these extrinsically sourced pathways, we now identify a regulatory pathway that is intrinsic to the core adhesion machinery, p...

journal_title：Molecular biology of the cell

authors： Li Z,Lock JG,Olofsson H,Kowalewski JM,Teller S,Liu Y,Zhang H,Strömblad S

更新日期：2010-10-01 00:00:00

abstract：:Ghrelin is an acylated peptidyl gastric hormone acting on the pituitary and hypothalamus to stimulate appetite, adiposity, and growth hormone release, through activation of growth hormone secretagogue receptor (GHSR)-1a receptor. Moreover, ghrelin features several activities such as inhibition of apoptosis, regulation...

journal_title：Molecular biology of the cell

authors： Filigheddu N,Gnocchi VF,Coscia M,Cappelli M,Porporato PE,Taulli R,Traini S,Baldanzi G,Chianale F,Cutrupi S,Arnoletti E,Ghè C,Fubini A,Surico N,Sinigaglia F,Ponzetto C,Muccioli G,Crepaldi T,Graziani A

更新日期：2007-03-01 00:00:00

abstract：:ARVCF, an armadillo-repeat protein of the p120(ctn) family, associates with classical cadherins and is present in adherens junctions, but its function is poorly understood. Here, we show that ARVCF interacts via a C-terminal PDZ-binding motif with zonula occludens (ZO)-1 and ZO-2. ARVCF and ZO-1 partially colocalize i...

journal_title：Molecular biology of the cell

authors： Kausalya PJ,Phua DC,Hunziker W

更新日期：2004-12-01 00:00:00

abstract：:Timely down-regulation of the evolutionarily conserved protein kinase Swe1 plays an important role in cell cycle control, as Swe1 can block nuclear division through inhibitory phosphorylation of the catalytic subunit of cyclin-dependent kinase. In particular, Swe1 degradation is important for budding yeast cell surviv...

journal_title：Molecular biology of the cell

authors： Raspelli E,Cassani C,Lucchini G,Fraschini R

更新日期：2011-07-01 00:00:00

abstract：:Tetraploidization, or genome doubling, is a prominent event in tumorigenesis, primarily because cell division in polyploid cells is error-prone and produces aneuploid cells. This study investigates changes in gene expression evoked in acute and adapted tetraploid cells and their effect on cell-cycle progression. Acute...

journal_title：Molecular biology of the cell

authors： Potapova TA,Seidel CW,Box AC,Rancati G,Li R

更新日期：2016-10-15 00:00:00

abstract：:The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule lo...

journal_title：Molecular biology of the cell

authors： Hermann GJ,Schroeder LK,Hieb CA,Kershner AM,Rabbitts BM,Fonarev P,Grant BD,Priess JR

更新日期：2005-07-01 00:00:00

abstract：:Nuclear migration is required for normal development in both higher and lower eukaryotes. In fungi this process is mediated by cytoplasmic dynein. It is believed that this motor protein is anchored to the cell membrane and moves nuclei by capturing and pulling on spindle pole body microtubules. To date, four genes hav...

journal_title：Molecular biology of the cell

authors： Chiu YH,Xiang X,Dawe AL,Morris NR

更新日期：1997-09-01 00:00:00

abstract：:The preprotein translocase of the outer mitochondrial membrane (TOM) consists of a central β-barrel channel, Tom40, and six proteins with α-helical transmembrane segments. The precursor of Tom40 is imported from the cytosol by a pre-existing TOM complex and inserted into the outer membrane by the sorting and assembly ...

journal_title：Molecular biology of the cell

authors： Becker T,Guiard B,Thornton N,Zufall N,Stroud DA,Wiedemann N,Pfanner N

更新日期：2010-09-15 00:00:00

abstract：:Ubiquitinated integral membrane proteins are delivered to the interior of the lysosome/vacuole for degradation. This process relies on specific ubiquitination of potential cargo and recognition of that Ub-cargo by sorting receptors at multiple compartments. We show that the endosomal Hse1-Vps27 sorting receptor binds ...

journal_title：Molecular biology of the cell

authors： Ren J,Kee Y,Huibregtse JM,Piper RC

更新日期：2007-01-01 00:00:00

abstract：:The c-myb proto-oncogene product (c-Myb) regulates proliferation and differentiation of hematopoietic cells. Recently we have shown that c-Myb is degraded in response to Wnt-1 stimulation via a pathway involving TAK1 (TGF-beta-activated kinase), HIPK2 (homeodomain-interacting protein kinase 2), and NLK (Nemo-like kina...

journal_title：Molecular biology of the cell

authors： Kurahashi T,Nomura T,Kanei-Ishii C,Shinkai Y,Ishii S

更新日期：2005-10-01 00:00:00

abstract：:Topogenic determinants that direct protein topology at the endoplasmic reticulum membrane usually function with high fidelity to establish a uniform topological orientation for any given polypeptide. Here we show, however, that through the coupling of sequential translocation events, native topogenic determinants are ...

journal_title：Molecular biology of the cell

authors： Moss K,Helm A,Lu Y,Bragin A,Skach WR

更新日期：1998-09-01 00:00:00

abstract：:Clearance of misfolded proteins from the ER is central for maintenance of cellular homeostasis. This process requires coordinated recognition, ER-cytosol translocation, and finally ubiquitination-dependent proteasomal degradation. Here, we identify an ER resident seven-transmembrane protein (JAMP) that links ER chaper...

journal_title：Molecular biology of the cell

authors： Tcherpakov M,Broday L,Delaunay A,Kadoya T,Khurana A,Erdjument-Bromage H,Tempst P,Qiu XB,DeMartino GN,Ronai Z

更新日期：2008-11-01 00:00:00

abstract：:The kinesin superfamily of microtubule motor proteins is important in many cellular processes, including mitosis and meiosis, vesicle transport, and the establishment and maintenance of cell polarity. We have characterized two related kinesins in fission yeast, klp5+ and klp6+,, that are amino-terminal motors of the K...

journal_title：Molecular biology of the cell

authors： West RR,Malmstrom T,Troxell CL,McIntosh JR

更新日期：2001-12-01 00:00:00

abstract：:The plant toxin ricin is transported retrogradely from the cell surface to the endoplasmic reticulum (ER) from where the enzymatically active part is retrotranslocated to the cytosol, presumably by the same mechanism as used by misfolded proteins. The ER degradation enhancing alpha-mannosidase I-like protein, EDEM, is...

journal_title：Molecular biology of the cell

authors： Slominska-Wojewodzka M,Gregers TF,Wälchli S,Sandvig K

更新日期：2006-04-01 00:00:00

abstract：:Silica inhalation leads to the development of the chronic lung disease silicosis. Macrophages are killed by uptake of nonopsonized silica particles, and this is believed to play a critical role in the etiology of silicosis. However, the mechanism of nonopsonized-particle uptake is not well understood. We compared the ...

journal_title：Molecular biology of the cell

authors： Gilberti RM,Knecht DA

更新日期：2015-02-01 00:00:00