Association of ARVCF with zonula occludens (ZO)-1 and ZO-2: binding to PDZ-domain proteins and cell-cell adhesion regulate plasma membrane and nuclear localization of ARVCF.

abstract：:The Rab11 effector Rab11-family interacting protein 2 (Rab11-FIP2) regulates transcytosis through its interactions with Rab11a and myosin Vb. Previous studies implicated Rab11-FIP2 in the establishment of polarity in Madin-Darby canine kidney (MDCK) cells through phosphorylation of Ser-227 by MARK2. Here we examine th...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Avant KM,Caldwell CM,Oztan A,Apodaca G,Knowles BC,Roland JT,Ducharme NA,Goldenring JR

更新日期：2012-06-01 00:00:00

abstract：:Zonula occludens (ZO)-1/2/3 are the members of the TJ-MAGUK family of membrane-associated guanylate kinases associated with tight junctions. To investigate the role of ZO-1 (encoded by Tjp1) in vivo, ZO-1 knockout (Tjp1(-/-)) mice were generated by gene targeting. Although heterozygous mice showed normal development a...

journal_title：Molecular biology of the cell

authors： Katsuno T,Umeda K,Matsui T,Hata M,Tamura A,Itoh M,Takeuchi K,Fujimori T,Nabeshima Y,Noda T,Tsukita S,Tsukita S

更新日期：2008-06-01 00:00:00

abstract：:Tumor necrosis factor α (TNF-α) receptor-associated factor 2 (TRAF2) regulates activation of the c-Jun N-terminal kinase (JNK)/c-Jun and the inhibitor of κB kinase (IKK)/nuclear factor κB (NF-κB) signaling cascades in response to TNF-α stimulation. Gene knockout studies have revealed that TRAF2 inhibits TNF-α-induced ...

journal_title：Molecular biology of the cell

authors： Zhang L,Blackwell K,Altaeva A,Shi Z,Habelhah H

更新日期：2011-01-01 00:00:00

abstract：:Activation of the c-myc proto-oncogene by chromosomal translocation or proviral insertion frequently results in the separation of the c-myc coding region from its normal regulatory elements. Such rearrangements are often accompanied by loss or mutation of c-myc exon 1 sequences. These genetic alterations do not affect...

journal_title：Molecular biology of the cell

authors： Blackwood EM,Lugo TG,Kretzner L,King MW,Street AJ,Witte ON,Eisenman RN

更新日期：1994-05-01 00:00:00

abstract：:In the present study we have identified a new metalloprotease encoded by the nuclear ATP23 gene of Saccharomyces cerevisiae that is essential for expression of mitochondrial ATPase (F(1)-F(O) complex). Mutations in ATP23 cause the accumulation of the precursor form of subunit 6 and prevent assembly of F(O). Atp23p is ...

journal_title：Molecular biology of the cell

authors： Zeng X,Neupert W,Tzagoloff A

更新日期：2007-02-01 00:00:00

abstract：:Cytokinesis in many eukaryotes is dependent on a contractile actomyosin ring (AMR), composed of F-actin, myosin II, and other actin and myosin II regulators. Through fluorescence recovery after photobleaching experiments, many components of the AMR have been shown to be mobile and to undergo constant exchange with the...

journal_title：Molecular biology of the cell

authors： Kamnev A,Palani S,Zambon P,Cheffings T,Burroughs N,Balasubramanian MK

更新日期：2021-02-01 00:00:00

abstract：:DEAD box proteins are putative RNA helicases that function in all aspects of RNA metabolism, including translation, ribosome biogenesis, and pre-mRNA splicing. Because many processes involving RNA metabolism are spatially organized within the cell, we examined the subcellular distribution of a human DEAD box protein, ...

journal_title：Molecular biology of the cell

authors： Bléoo S,Sun X,Hendzel MJ,Rowe JM,Packer M,Godbout R

更新日期：2001-10-01 00:00:00

abstract：:Phospholipase D (PLD) has been suggested to mediate epidermal growth factor (EGF) signaling. However, the molecular mechanism of EGF-induced PLD activation has not yet been elucidated. We investigated the importance of the phosphorylation and compartmentalization of PLD1 in EGF signaling. EGF treatment of COS-7 cells ...

journal_title：Molecular biology of the cell

authors： Han JM,Kim Y,Lee JS,Lee CS,Lee BD,Ohba M,Kuroki T,Suh PG,Ryu SH

更新日期：2002-11-01 00:00:00

abstract：:Various human diseases are associated with mitochondrial DNA (mtDNA) mutations, but heteroplasmy—the coexistence of mutant and wild-type mtDNA—complicates their study. We previously isolated a temperature-lethal mtDNA mutation in Drosophila, mt:CoI(T300I), which affects the cytochrome c oxidase subunit I (CoI) locus. ...

journal_title：Molecular biology of the cell

authors： Chen Z,Qi Y,French S,Zhang G,Covian Garcia R,Balaban R,Xu H

更新日期：2015-02-15 00:00:00

abstract：:Two cDNAs encoding casein kinase-1 have been isolated from a yeast cDNA library and termed CKI1 and CKI2. Each clone encodes a protein of approximately 62,000 Da containing a highly conserved protein kinase domain surrounded by variable amino- and carboxy-terminal domains. The proteins also contain two conserved carbo...

journal_title：Molecular biology of the cell

authors： Wang PC,Vancura A,Mitcheson TG,Kuret J

更新日期：1992-03-01 00:00:00

abstract：:Rab proteins are thought to function in the processes by which transport vesicles identify and/or fuse with their respective target membranes. The bulk of these proteins are membrane associated, but a measurable fraction can be found in the cytosol. The cytosolic forms of rab3A, rab11, and Sec4 occur as equimolar comp...

journal_title：Molecular biology of the cell

authors： Soldati T,Riederer MA,Pfeffer SR

更新日期：1993-04-01 00:00:00

abstract：:Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobo...

journal_title：Molecular biology of the cell

authors： Sao K,Jones TM,Doyle AD,Maity D,Schevzov G,Chen Y,Gunning PW,Petrie RJ

更新日期：2019-05-01 00:00:00

abstract：:Mitochondrial function depends crucially on the maintenance of multiple mitochondrial DNA (mtDNA) copies. Surprisingly, the cellular mechanisms regulating mtDNA copy number remain poorly understood. Through a systematic high-throughput approach in Saccharomyces cerevisiae, we determined mtDNA-to-nuclear DNA ratios in ...

journal_title：Molecular biology of the cell

authors： Göke A,Schrott S,Mizrak A,Belyy V,Osman C,Walter P

更新日期：2020-03-19 00:00:00

abstract：:The CCAAT/enhancer binding protein delta (C/EBPdelta, CRP3, CELF, NF-IL6beta) regulates gene expression and plays functional roles in many tissues, such as in acute phase response to inflammatory stimuli, adipocyte differentiation, and mammary epithelial cell growth control. In this study, we examined the expression o...

journal_title：Molecular biology of the cell

authors： Wang JM,Tseng JT,Chang WC

更新日期：2005-07-01 00:00:00

abstract：:Cyclin D1 expression is jointly regulated by growth factors and cell adhesion to the extracellular matrix in many cell types. Growth factors are thought to regulate cyclin D1 expression because they stimulate sustained extracellular signal-regulated kinase (ERK) activity. However, we show here that growth factors indu...

journal_title：Molecular biology of the cell

authors： Roovers K,Davey G,Zhu X,Bottazzi ME,Assoian RK

更新日期：1999-10-01 00:00:00

abstract：:The early Caenorhabditis elegans embryo contains abundant transcripts for two alpha- and two beta-tubulins, raising the question of whether each isoform performs specialized functions or simply contributes to total tubulin levels. Our identification of two recessive, complementing alleles of a beta-tubulin that disrup...

journal_title：Molecular biology of the cell

authors： Wright AJ,Hunter CP

更新日期：2003-11-01 00:00:00

abstract：:Cyclin-dependent kinase (Cdk)2/cyclin E is imported into nuclei assembled in Xenopus egg extracts by a pathway that requires importin-alpha and -beta. Here, we identify a basic nuclear localization sequence (NLS) in the N-terminus of Xenopus cyclin E. Mutation of the NLS eliminated nuclear accumulation of both cyclin ...

journal_title：Molecular biology of the cell

authors： Moore JD,Kornbluth S,Hunt T

更新日期：2002-12-01 00:00:00

abstract：:The majority of mitochondrial phosphatidylethanolamine (PtdEtn), a phospholipid essential for aerobic growth of yeast cells, is synthesized by phosphatidylserine decarboxylase 1 (Psd1p) in the inner mitochondrial membrane (IMM). To identify components that become essential when the level of mitochondrial PtdEtn is dec...

journal_title：Molecular biology of the cell

authors： Birner R,Nebauer R,Schneiter R,Daum G

更新日期：2003-02-01 00:00:00

abstract：:Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). The anaphase-promoting complex or cyclosome (APC/C) comprises a multisubunit ubiquitin l...

journal_title：Molecular biology of the cell

authors： Leverson JD,Joazeiro CA,Page AM,Huang Hk,Hieter P,Hunter T

更新日期：2000-07-01 00:00:00

abstract：:Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the clathrin adaptor complex AP-1 is involved in retroviral release. The absence of AP-1mu obtained by genetic knock-out or b...

journal_title：Molecular biology of the cell

authors： Camus G,Segura-Morales C,Molle D,Lopez-Vergès S,Begon-Pescia C,Cazevieille C,Schu P,Bertrand E,Berlioz-Torrent C,Basyuk E

更新日期：2007-08-01 00:00:00

abstract：:I am extremely honored to be the recipient of the 2015 Women in Cell Biology Junior Award. When I reflect on my journey in science, many great people and memorable experiences come to mind. Some of these encounters were truly career-defining moments. Others provided priceless lessons. In this essay, I recount some of ...

journal_title：Molecular biology of the cell

authors： Serpe M

更新日期：2015-11-01 00:00:00

abstract：:The putative role of sorting early endosomes (EEs) in synaptic-like microvesicle (SLMV) formation in the neuroendocrine PC12 cell line was investigated by quantitative immunoelectron microscopy. By BSA-gold internalization kinetics, four distinct endosomal subcompartments were distinguished: primary endocytic vesicles...

journal_title：Molecular biology of the cell

authors： de Wit H,Lichtenstein Y,Geuze HJ,Kelly RB,van der Sluijs P,Klumperman J

更新日期：1999-12-01 00:00:00

abstract：:Barrier to autointegration factor (BAF) is an essential component of the nuclear lamina that binds lamins, LEM-domain proteins, histones, and DNA. Under normal conditions, BAF protein is highly mobile when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching. We report that Cae...

journal_title：Molecular biology of the cell

authors： Bar DZ,Davidovich M,Lamm AT,Zer H,Wilson KL,Gruenbaum Y

更新日期：2014-04-01 00:00:00

abstract：:Cholesterol is an essential constituent of membranes in mammalian cells. The plasma membrane and the endocytic recycling compartment (ERC) are both highly enriched in cholesterol. The abundance and distribution of cholesterol among organelles are tightly controlled by a combination of mechanisms involving vesicular an...

journal_title：Molecular biology of the cell

authors： Iaea DB,Mao S,Lund FW,Maxfield FR

更新日期：2017-04-15 00:00:00

abstract：:To further elucidate the functions of actin in budding yeast and to relate actin structure to specific roles and interactions in vivo, we determined the phenotypes caused by 13 charged-to-alanine mutations isolated previously in the single Saccharomyces cerevisiae actin gene. Defects in actin organization, morphogenes...

journal_title：Molecular biology of the cell

authors： Drubin DG,Jones HD,Wertman KF

更新日期：1993-12-01 00:00:00

abstract：:Although evasion of apoptosis is thought to be required for the development of cancer, it is unclear which cell death pathways are evaded. We previously identified a novel epithelial cell death pathway that works in normal cells but is inactivated in tumor cells, implying that it may be targeted during tumor developme...

journal_title：Molecular biology of the cell

authors： Thorburn J,Moore F,Rao A,Barclay WW,Thomas LR,Grant KW,Cramer SD,Thorburn A

更新日期：2005-03-01 00:00:00

abstract：:Binding of echovirus 1 (EV1, a nonenveloped RNA virus) to the alpha2beta1 integrin on the cell surface is followed by endocytic internalization of the virus together with the receptor. Here, video-enhanced live microscopy revealed the rapid uptake of fluorescently labeled EV1 into mobile, intracellular structures, pos...

journal_title：Molecular biology of the cell

authors： Pietiäinen V,Marjomäki V,Upla P,Pelkmans L,Helenius A,Hyypiä T

更新日期：2004-11-01 00:00:00

abstract：:The yeast [PSI+] prion is an epigenetic modifier of translation termination fidelity that causes nonsense suppression. The prion [PSI+] forms when the translation termination factor Sup35p adopts a self-propagating conformation. The presence of the [PSI+] prion modulates survivability in a variety of growth conditions...

journal_title：Molecular biology of the cell

authors： Strawn LA,Lin CA,Tank EM,Osman MM,Simpson SA,True HL

更新日期：2009-04-01 00:00:00

abstract：:Topogenic determinants that direct protein topology at the endoplasmic reticulum membrane usually function with high fidelity to establish a uniform topological orientation for any given polypeptide. Here we show, however, that through the coupling of sequential translocation events, native topogenic determinants are ...

journal_title：Molecular biology of the cell

authors： Moss K,Helm A,Lu Y,Bragin A,Skach WR

更新日期：1998-09-01 00:00:00

abstract：:B cell antigen receptor (BCR) association with lipid rafts, the actin cytoskeleton, and clathrin-coated pits influences B cell signaling and antigen presentation. Although all three cellular structures have been separately implicated in BCR internalization, the relationship between them has not been clearly defined. I...

journal_title：Molecular biology of the cell

authors： Stoddart A,Jackson AP,Brodsky FM

更新日期：2005-05-01 00:00:00