JAMP optimizes ERAD to protect cells from unfolded proteins.

abstract：:S100A4, a member of the S100 family of Ca(2+)-binding proteins, is directly involved in tumor metastasis. In addition to its expression in tumor cells, S100A4 is expressed in normal cells and tissues, including fibroblasts and cells of the immune system. To examine the contribution of S100A4 to normal physiology, we e...

journal_title：Molecular biology of the cell

authors： Li ZH,Dulyaninova NG,House RP,Almo SC,Bresnick AR

更新日期：2010-08-01 00:00:00

abstract：:The daily phagocytosis of shed photoreceptor outer segments by pigment epithelial cells is critical for the maintenance of the retina. In a subtractive polymerase chain reaction analysis, we found that functional differentiation of human ARPE19 retinal pigment epithelial (RPE) cells is accompanied by up-regulation of ...

journal_title：Molecular biology of the cell

authors： Law AL,Ling Q,Hajjar KA,Futter CE,Greenwood J,Adamson P,Wavre-Shapton ST,Moss SE,Hayes MJ

更新日期：2009-09-01 00:00:00

abstract：:The nuclear lamina is a key structural element of the metazoan nucleus. However, the structural organization of the major proteins composing the lamina is poorly defined. Using three-dimensional structured illumination microscopy and computational image analysis, we characterized the supramolecular structures of lamin...

journal_title：Molecular biology of the cell

authors： Shimi T,Kittisopikul M,Tran J,Goldman AE,Adam SA,Zheng Y,Jaqaman K,Goldman RD

更新日期：2015-11-05 00:00:00

abstract：:Trimming of N-linked oligosaccharides by endoplasmic reticulum (ER) glucosidase II is implicated in quality control of protein folding. An alternate glucosidase II-independent deglucosylation pathway exists, in which endo-alpha-mannosidase cleaves internally the glucose-substituted mannose residue of oligosaccharides....

journal_title：Molecular biology of the cell

authors： Zuber C,Spiro MJ,Guhl B,Spiro RG,Roth J

更新日期：2000-12-01 00:00:00

abstract：:We previously characterized major components of mitotic chromosomes assembled in Xenopus laevis egg extracts and collectively referred to them as Xenopus chromosome-associated polypeptides (XCAPs). They included five subunits of the condensin complex essential for chromosome condensation. In an effort to identify nove...

journal_title：Molecular biology of the cell

authors： MacCallum DE,Losada A,Kobayashi R,Hirano T

更新日期：2002-01-01 00:00:00

abstract：:Coordinated regulation of cell proliferation is vital for epithelial tissue homeostasis, and uncontrolled proliferation is a hallmark of carcinogenesis. A growing body of evidence indicates that epithelial tight junctions (TJs) play a role in these processes, although the mechanisms involved are poorly understood. In ...

journal_title：Molecular biology of the cell

authors： Capaldo CT,Koch S,Kwon M,Laur O,Parkos CA,Nusrat A

更新日期：2011-05-15 00:00:00

abstract：:The Rab11 effector Rab11-family interacting protein 2 (Rab11-FIP2) regulates transcytosis through its interactions with Rab11a and myosin Vb. Previous studies implicated Rab11-FIP2 in the establishment of polarity in Madin-Darby canine kidney (MDCK) cells through phosphorylation of Ser-227 by MARK2. Here we examine th...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Avant KM,Caldwell CM,Oztan A,Apodaca G,Knowles BC,Roland JT,Ducharme NA,Goldenring JR

更新日期：2012-06-01 00:00:00

abstract：:We used immunofluorescence to study the distribution and targeting of RNA polymerase (pol) III subunits and pol III transcription factors in the Xenopus laevis oocyte nucleus. Antibodies against several of these proteins stained Cajal bodies and approximately 90 specific sites on the lampbrush chromosomes. Some of the...

journal_title：Molecular biology of the cell

authors： Murphy C,Wang Z,Roeder RG,Gall JG

更新日期：2002-10-01 00:00:00

abstract：:Treacher Collins syndrome (TCS) is an autosomal dominant disorder of craniofacial development caused by mutations in the gene TCOF1. Its gene product, treacle, consists mainly of a central repeat domain, which shows it to be structurally related to the nucleolar phosphoprotein Nopp140. Treacle remains mostly uncharact...

journal_title：Molecular biology of the cell

authors： Isaac C,Marsh KL,Paznekas WA,Dixon J,Dixon MJ,Jabs EW,Meier UT

更新日期：2000-09-01 00:00:00

abstract：:The formation and maintenance of the bipolar mitotic spindle apparatus require a complex and balanced interplay of several mechanisms, including the stabilization and separation of polar microtubules and the action of various microtubule motors. Nonmicrotubule elements are also present throughout the spindle apparatus...

journal_title：Molecular biology of the cell

authors： Yang CH,Snyder M

更新日期：1992-11-01 00:00:00

abstract：:In biogenesis of membrane proteins on the endoplasmic reticulum, a protein-conducting channel called the translocon functions in both the membrane translocation of lumenal domains and the integration of transmembrane segments. Here we analyzed the environments of polypeptide chains during the processes by water-depend...

journal_title：Molecular biology of the cell

authors： Kida Y,Kume C,Hirano M,Sakaguchi M

更新日期：2010-02-01 00:00:00

abstract：:Since 2008, the autophagy community has periodically published a set of guidelines, currently titled "Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy." The newest version of the guidelines was published in 2016. There are many reasons for establishing a set of guidelines in a given researc...

journal_title：Molecular biology of the cell

authors： Klionsky DJ

更新日期：2016-03-01 00:00:00

abstract：:Five or more orthophosphates bound together by high-energy phosphoanhydride bonds are highly ubiquitous inorganic molecules called polyphosphate. Polyphosphate acts as a signaling molecule eliciting a number of responses in eukaryotic cells, but the mechanisms mediating these effects are poorly understood. Proliferati...

journal_title：Molecular biology of the cell

authors： Suess PM,Tang Y,Gomer RH

更新日期：2019-04-15 00:00:00

abstract：:Overexpression of the inhibitory Smad, Smad7, is used frequently to implicate the Smad pathway in cellular responses to transforming growth factor beta (TGF-beta) signaling; however, Smad7 regulates several other proteins, including Cdc42, p38MAPK, and beta-catenin. We report an alternative approach for more specifica...

journal_title：Molecular biology of the cell

authors： Zhao BM,Hoffmann FM

更新日期：2006-09-01 00:00:00

abstract：:The key molecular regulatory mechanisms that govern and coordinate the molecular alterations that underpin the process of human labor remain incompletely understood although enhanced intrauterine prostaglandin production is known to be requisite. Studies from cancer tissues have indicated that at least one key enzyme ...

journal_title：Molecular biology of the cell

authors： Mitchell MD

更新日期：2006-01-01 00:00:00

abstract：:What started as a game of discovery for a child with a chemistry set has resulted in a lifetime of fulfillment and dedication to science. It is deeply rewarding to continuously let my curiosity ask questions for which there are no known answers. Equally rewarding has been the opportunity to mentor young students and p...

journal_title：Molecular biology of the cell

authors： Garcia JV

更新日期：2013-11-01 00:00:00

abstract：:The majority of mitochondrial phosphatidylethanolamine (PtdEtn), a phospholipid essential for aerobic growth of yeast cells, is synthesized by phosphatidylserine decarboxylase 1 (Psd1p) in the inner mitochondrial membrane (IMM). To identify components that become essential when the level of mitochondrial PtdEtn is dec...

journal_title：Molecular biology of the cell

authors： Birner R,Nebauer R,Schneiter R,Daum G

更新日期：2003-02-01 00:00:00

abstract：:The Saccharomyces cerevisiae a-factor receptor (Ste3p) requires its C-terminal cytoplasmic tail for endocytosis. Wild-type receptor is delivered to the cell surface via the secretory pathway but remains there only briefly before being internalized and delivered to the vacuole for degradation. Receptors lacking all or ...

journal_title：Molecular biology of the cell

authors： Givan SA,Sprague GF Jr

更新日期：1997-07-01 00:00:00

abstract：:Alternative pre-mRNA splicing patterns can change an extracellular stimulus, but the signaling pathways leading to these changes are still poorly characterized. Here, we describe a tyrosine-phosphorylated nuclear protein, YT521-B, and show that it interacts with the nuclear transcriptosomal component scaffold attachme...

journal_title：Molecular biology of the cell

authors： Hartmann AM,Nayler O,Schwaiger FW,Obermeier A,Stamm S

更新日期：1999-11-01 00:00:00

abstract：:Podosomes are dynamic actin-rich structures composed of a dense F-actin core surrounded by a cloud of more diffuse F-actin. Src performs one or more unique functions in osteoclasts (OCLs), and podosome belts and bone resorption are impaired in the absence of Src. Using Src(-/-) OCLs, we investigated the specific funct...

journal_title：Molecular biology of the cell

authors： Destaing O,Sanjay A,Itzstein C,Horne WC,Toomre D,De Camilli P,Baron R

更新日期：2008-01-01 00:00:00

abstract：:Previous research has found that a gamma-tubulin mutation in Schizosaccharomyces pombe is synthetically lethal with a deletion of the C-terminal motor domain kinesin-like protein gene pkl1, but the lethality of the double mutant prevents a phenotypic analysis of the synthetic interaction. We have investigated interact...

journal_title：Molecular biology of the cell

authors： Prigozhina NL,Walker RA,Oakley CE,Oakley BR

更新日期：2001-10-01 00:00:00

abstract：:MPM-2 antigens, a discrete set of phosphoproteins that contain similar phosphoepitopes recognized by the monoclonal antibody MPM-2, are phosphorylated during M-phase induction. Our previous studies suggested that certain MPM-2 antigens are involved in the appearance of maturation-promoting factor (MPF) activity. Becau...

journal_title：Molecular biology of the cell

authors： Kuang J,Ashorn CL,Gonzalez-Kuyvenhoven M,Penkala JE

更新日期：1994-02-01 00:00:00

abstract：:Loss-of-function mutations in the nonconventional myosin Vb (Myo5b) result in microvillus inclusion disease (MVID) and massive secretory diarrhea that often begins at birth. Myo5b mutations disrupt the apical recycling endosome (ARE) and membrane traffic, resulting in reduced surface expression of apical membrane prot...

journal_title：Molecular biology of the cell

authors： Forteza R,Ahsan MK,Cartón-García F,Arango D,Ameen NA,Salas PJ

更新日期：2019-12-15 00:00:00

abstract：:Previously, we showed that spliceosomal U6 small nuclear RNA (snRNA) transiently passes through the nucleolus. Herein, we report that all individual snRNAs of the [U4/U6.U5] tri-snRNP localize to nucleoli, demonstrated by fluorescence microscopy of nucleolar preparations after injection of fluorescein-labeled snRNA in...

journal_title：Molecular biology of the cell

authors： Gerbi SA,Lange TS

更新日期：2002-09-01 00:00:00

abstract：:The engagement of integrin alpha7 in E63 skeletal muscle cells by laminin or anti-alpha7 antibodies triggered transient elevations in the intracellular free Ca(2+) concentration that resulted from both inositol triphosphate-evoked Ca(2+) release from intracellular stores and extracellular Ca(2+) influx through voltage...

journal_title：Molecular biology of the cell

authors： Kwon MS,Park CS,Choi K,Ahnn J,Kim JI,Eom SH,Kaufman SJ,Song WK

更新日期：2000-04-01 00:00:00

abstract：:The transition between the closed and open conformations of the Sec61 complex permits nascent protein insertion into the translocation channel. A critical event in this structural transition is the opening of the lateral translocon gate that is formed by four transmembrane (TM) spans (TM2, TM3, TM7, and TM8 in Sec61p)...

journal_title：Molecular biology of the cell

authors： Trueman SF,Mandon EC,Gilmore R

更新日期：2011-09-01 00:00:00

abstract：:GRIM-19 was found to copurify with complex I of mitochondrial respiratory chain and subsequently was demonstrated to be involved in complex I assembly and activity. To further understand its function in complex I, we dissected its functional domains by generating a number of deletion, truncation, and point mutants. Th...

journal_title：Molecular biology of the cell

authors： Lu H,Cao X

更新日期：2008-05-01 00:00:00

abstract：:Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate r...

journal_title：Molecular biology of the cell

authors： Holenstein CN,Horvath A,Schär B,Schoenenberger AD,Bollhalder M,Goedecke N,Bartalena G,Otto O,Herbig M,Guck J,Müller DA,Snedeker JG,Silvan U

更新日期：2019-03-21 00:00:00

abstract：:The Sec61 translocon mediates the translocation of proteins across the endoplasmic reticulum membrane and the lateral integration of transmembrane segments into the lipid bilayer. The structure of the idle translocon is closed by a lumenal plug domain and a hydrophobic constriction ring. To test the function of the ap...

journal_title：Molecular biology of the cell

authors： Junne T,Kocik L,Spiess M

更新日期：2010-05-15 00:00:00

abstract：:Yeast TGN resident proteins that frequently cycle between the TGN and endosomes are much more slowly transported to the prevacuolar/late endosomal compartment (PVC) than other proteins. However, TGN protein transport to the PVC is accelerated in mutants lacking function of Inp53p. Inp53p contains a SacI polyphosphoino...

journal_title：Molecular biology of the cell

authors： Ha SA,Torabinejad J,DeWald DB,Wenk MR,Lucast L,De Camilli P,Newitt RA,Aebersold R,Nothwehr SF

更新日期：2003-04-01 00:00:00